Stecher B 801 Horn M
Stecher B 801 Daims H
Stecher B 801 Berry D
Stecher B 801 Loy A
Stecher B 801 Wagner M
Stecher B 801 Eichorst SA
Stecher B 801 Mussmann M
Stecher B 801 Wasmund K
Stecher B 801 Herbold CW
Stecher B 801 Sedlacek CJ
Clavel T 728 Horn M
Clavel T 728 Daims H
Clavel T 728 Berry D
Clavel T 728 Loy A
Clavel T 728 Wagner M
Clavel T 728 Eichorst SA
Clavel T 728 Mussmann M
Clavel T 728 Wasmund K
Clavel T 728 Herbold CW
Clavel T 728 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Naughton LM 1914 Horn M
Naughton LM 1914 Daims H
Naughton LM 1914 Berry D
Naughton LM 1914 Loy A
Naughton LM 1914 Wagner M
Naughton LM 1914 Eichorst SA
Naughton LM 1914 Mussmann M
Naughton LM 1914 Wasmund K
Naughton LM 1914 Herbold CW
Naughton LM 1914 Sedlacek CJ
Romano S 1910 Horn M
Romano S 1910 Daims H
Romano S 1910 Berry D
Romano S 1910 Loy A
Romano S 1910 Wagner M
Romano S 1910 Eichorst SA
Romano S 1910 Mussmann M
Romano S 1910 Wasmund K
Romano S 1910 Herbold CW
Romano S 1910 Sedlacek CJ
O'Gara F 1469 Horn M
O'Gara F 1469 Daims H
O'Gara F 1469 Berry D
O'Gara F 1469 Loy A
O'Gara F 1469 Wagner M
O'Gara F 1469 Eichorst SA
O'Gara F 1469 Mussmann M
O'Gara F 1469 Wasmund K
O'Gara F 1469 Herbold CW
O'Gara F 1469 Sedlacek CJ
Dobson ADW 1915 Horn M
Dobson ADW 1915 Daims H
Dobson ADW 1915 Berry D
Dobson ADW 1915 Loy A
Dobson ADW 1915 Wagner M
Dobson ADW 1915 Eichorst SA
Dobson ADW 1915 Mussmann M
Dobson ADW 1915 Wasmund K
Dobson ADW 1915 Herbold CW
Dobson ADW 1915 Sedlacek CJ
Böck D 1905 Horn M
Böck D 1905 Daims H
Böck D 1905 Berry D
Böck D 1905 Loy A
Böck D 1905 Wagner M
Böck D 1905 Eichorst SA
Böck D 1905 Mussmann M
Böck D 1905 Wasmund K
Böck D 1905 Herbold CW
Böck D 1905 Sedlacek CJ
Medeiros JM 1906 Horn M
Medeiros JM 1906 Daims H
Medeiros JM 1906 Berry D
Medeiros JM 1906 Loy A
Medeiros JM 1906 Wagner M
Medeiros JM 1906 Eichorst SA
Medeiros JM 1906 Mussmann M
Medeiros JM 1906 Wasmund K
Medeiros JM 1906 Herbold CW
Medeiros JM 1906 Sedlacek CJ
Tsao HF 1704 Horn M
Tsao HF 1704 Daims H
Tsao HF 1704 Berry D
Tsao HF 1704 Loy A
Tsao HF 1704 Wagner M
Tsao HF 1704 Eichorst SA
Tsao HF 1704 Mussmann M
Tsao HF 1704 Wasmund K
Tsao HF 1704 Herbold CW
Tsao HF 1704 Sedlacek CJ
Penz T 779 Horn M
Penz T 779 Daims H
Penz T 779 Berry D
Penz T 779 Loy A
Penz T 779 Wagner M
Penz T 779 Eichorst SA
Penz T 779 Mussmann M
Penz T 779 Wasmund K
Penz T 779 Herbold CW
Penz T 779 Sedlacek CJ
Weiss GL 1907 Horn M
Weiss GL 1907 Daims H
Weiss GL 1907 Berry D
Weiss GL 1907 Loy A
Weiss GL 1907 Wagner M
Weiss GL 1907 Eichorst SA
Weiss GL 1907 Mussmann M
Weiss GL 1907 Wasmund K
Weiss GL 1907 Herbold CW
Weiss GL 1907 Sedlacek CJ
Aistleitner K 258 Horn M
Aistleitner K 258 Daims H
Aistleitner K 258 Berry D
Aistleitner K 258 Loy A
Aistleitner K 258 Wagner M
Aistleitner K 258 Eichorst SA
Aistleitner K 258 Mussmann M
Aistleitner K 258 Wasmund K
Aistleitner K 258 Herbold CW
Aistleitner K 258 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Pilhofer M 540 Horn M
Pilhofer M 540 Daims H
Pilhofer M 540 Berry D
Pilhofer M 540 Loy A
Pilhofer M 540 Wagner M
Pilhofer M 540 Eichorst SA
Pilhofer M 540 Mussmann M
Pilhofer M 540 Wasmund K
Pilhofer M 540 Herbold CW
Pilhofer M 540 Sedlacek CJ
Collingro A 293 Horn M
Collingro A 293 Daims H
Collingro A 293 Berry D
Collingro A 293 Loy A
Collingro A 293 Wagner M
Collingro A 293 Eichorst SA
Collingro A 293 Mussmann M
Collingro A 293 Wasmund K
Collingro A 293 Herbold CW
Collingro A 293 Sedlacek CJ
Köstlbacher S 1893 Horn M
Köstlbacher S 1893 Daims H
Köstlbacher S 1893 Berry D
Köstlbacher S 1893 Loy A
Köstlbacher S 1893 Wagner M
Köstlbacher S 1893 Eichorst SA
Köstlbacher S 1893 Mussmann M
Köstlbacher S 1893 Wasmund K
Köstlbacher S 1893 Herbold CW
Köstlbacher S 1893 Sedlacek CJ
Mussmann M 896 Horn M
Mussmann M 896 Daims H
Mussmann M 896 Berry D
Mussmann M 896 Loy A
Mussmann M 896 Wagner M
Mussmann M 896 Eichorst SA
Mussmann M 896 Mussmann M
Mussmann M 896 Wasmund K
Mussmann M 896 Herbold CW
Mussmann M 896 Sedlacek CJ
Stepanauskas R 342 Horn M
Stepanauskas R 342 Daims H
Stepanauskas R 342 Berry D
Stepanauskas R 342 Loy A
Stepanauskas R 342 Wagner M
Stepanauskas R 342 Eichorst SA
Stepanauskas R 342 Mussmann M
Stepanauskas R 342 Wasmund K
Stepanauskas R 342 Herbold CW
Stepanauskas R 342 Sedlacek CJ
Hallam SJ 1894 Horn M
Hallam SJ 1894 Daims H
Hallam SJ 1894 Berry D
Hallam SJ 1894 Loy A
Hallam SJ 1894 Wagner M
Hallam SJ 1894 Eichorst SA
Hallam SJ 1894 Mussmann M
Hallam SJ 1894 Wasmund K
Hallam SJ 1894 Herbold CW
Hallam SJ 1894 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Tsao HF 1704 Horn M
Tsao HF 1704 Daims H
Tsao HF 1704 Berry D
Tsao HF 1704 Loy A
Tsao HF 1704 Wagner M
Tsao HF 1704 Eichorst SA
Tsao HF 1704 Mussmann M
Tsao HF 1704 Wasmund K
Tsao HF 1704 Herbold CW
Tsao HF 1704 Sedlacek CJ
Scheikl U 1703 Horn M
Scheikl U 1703 Daims H
Scheikl U 1703 Berry D
Scheikl U 1703 Loy A
Scheikl U 1703 Wagner M
Scheikl U 1703 Eichorst SA
Scheikl U 1703 Mussmann M
Scheikl U 1703 Wasmund K
Scheikl U 1703 Herbold CW
Scheikl U 1703 Sedlacek CJ
Volland JM 661 Horn M
Volland JM 661 Daims H
Volland JM 661 Berry D
Volland JM 661 Loy A
Volland JM 661 Wagner M
Volland JM 661 Eichorst SA
Volland JM 661 Mussmann M
Volland JM 661 Wasmund K
Volland JM 661 Herbold CW
Volland JM 661 Sedlacek CJ
Köhsler M 1892 Horn M
Köhsler M 1892 Daims H
Köhsler M 1892 Berry D
Köhsler M 1892 Loy A
Köhsler M 1892 Wagner M
Köhsler M 1892 Eichorst SA
Köhsler M 1892 Mussmann M
Köhsler M 1892 Wasmund K
Köhsler M 1892 Herbold CW
Köhsler M 1892 Sedlacek CJ
Bright M 262 Horn M
Bright M 262 Daims H
Bright M 262 Berry D
Bright M 262 Loy A
Bright M 262 Wagner M
Bright M 262 Eichorst SA
Bright M 262 Mussmann M
Bright M 262 Wasmund K
Bright M 262 Herbold CW
Bright M 262 Sedlacek CJ
Walochnik J 427 Horn M
Walochnik J 427 Daims H
Walochnik J 427 Berry D
Walochnik J 427 Loy A
Walochnik J 427 Wagner M
Walochnik J 427 Eichorst SA
Walochnik J 427 Mussmann M
Walochnik J 427 Wasmund K
Walochnik J 427 Herbold CW
Walochnik J 427 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
König L 542 Horn M
König L 542 Daims H
König L 542 Berry D
König L 542 Loy A
König L 542 Wagner M
König L 542 Eichorst SA
König L 542 Mussmann M
König L 542 Wasmund K
König L 542 Herbold CW
König L 542 Sedlacek CJ
Siegl A 871 Horn M
Siegl A 871 Daims H
Siegl A 871 Berry D
Siegl A 871 Loy A
Siegl A 871 Wagner M
Siegl A 871 Eichorst SA
Siegl A 871 Mussmann M
Siegl A 871 Wasmund K
Siegl A 871 Herbold CW
Siegl A 871 Sedlacek CJ
Penz T 779 Horn M
Penz T 779 Daims H
Penz T 779 Berry D
Penz T 779 Loy A
Penz T 779 Wagner M
Penz T 779 Eichorst SA
Penz T 779 Mussmann M
Penz T 779 Wasmund K
Penz T 779 Herbold CW
Penz T 779 Sedlacek CJ
Haider S 958 Horn M
Haider S 958 Daims H
Haider S 958 Berry D
Haider S 958 Loy A
Haider S 958 Wagner M
Haider S 958 Eichorst SA
Haider S 958 Mussmann M
Haider S 958 Wasmund K
Haider S 958 Herbold CW
Haider S 958 Sedlacek CJ
Wentrup C 1125 Horn M
Wentrup C 1125 Daims H
Wentrup C 1125 Berry D
Wentrup C 1125 Loy A
Wentrup C 1125 Wagner M
Wentrup C 1125 Eichorst SA
Wentrup C 1125 Mussmann M
Wentrup C 1125 Wasmund K
Wentrup C 1125 Herbold CW
Wentrup C 1125 Sedlacek CJ
Polzin J 1889 Horn M
Polzin J 1889 Daims H
Polzin J 1889 Berry D
Polzin J 1889 Loy A
Polzin J 1889 Wagner M
Polzin J 1889 Eichorst SA
Polzin J 1889 Mussmann M
Polzin J 1889 Wasmund K
Polzin J 1889 Herbold CW
Polzin J 1889 Sedlacek CJ
Mann E 1890 Horn M
Mann E 1890 Daims H
Mann E 1890 Berry D
Mann E 1890 Loy A
Mann E 1890 Wagner M
Mann E 1890 Eichorst SA
Mann E 1890 Mussmann M
Mann E 1890 Wasmund K
Mann E 1890 Herbold CW
Mann E 1890 Sedlacek CJ
Schmitz-Esser S 593 Horn M
Schmitz-Esser S 593 Daims H
Schmitz-Esser S 593 Berry D
Schmitz-Esser S 593 Loy A
Schmitz-Esser S 593 Wagner M
Schmitz-Esser S 593 Eichorst SA
Schmitz-Esser S 593 Mussmann M
Schmitz-Esser S 593 Wasmund K
Schmitz-Esser S 593 Herbold CW
Schmitz-Esser S 593 Sedlacek CJ
Domman D 299 Horn M
Domman D 299 Daims H
Domman D 299 Berry D
Domman D 299 Loy A
Domman D 299 Wagner M
Domman D 299 Eichorst SA
Domman D 299 Mussmann M
Domman D 299 Wasmund K
Domman D 299 Herbold CW
Domman D 299 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Lang M 1874 Horn M
Lang M 1874 Daims H
Lang M 1874 Berry D
Lang M 1874 Loy A
Lang M 1874 Wagner M
Lang M 1874 Eichorst SA
Lang M 1874 Mussmann M
Lang M 1874 Wasmund K
Lang M 1874 Herbold CW
Lang M 1874 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Passecker K 1875 Horn M
Passecker K 1875 Daims H
Passecker K 1875 Berry D
Passecker K 1875 Loy A
Passecker K 1875 Wagner M
Passecker K 1875 Eichorst SA
Passecker K 1875 Mussmann M
Passecker K 1875 Wasmund K
Passecker K 1875 Herbold CW
Passecker K 1875 Sedlacek CJ
Mesteri I 1876 Horn M
Mesteri I 1876 Daims H
Mesteri I 1876 Berry D
Mesteri I 1876 Loy A
Mesteri I 1876 Wagner M
Mesteri I 1876 Eichorst SA
Mesteri I 1876 Mussmann M
Mesteri I 1876 Wasmund K
Mesteri I 1876 Herbold CW
Mesteri I 1876 Sedlacek CJ
Bhuju S 1877 Horn M
Bhuju S 1877 Daims H
Bhuju S 1877 Berry D
Bhuju S 1877 Loy A
Bhuju S 1877 Wagner M
Bhuju S 1877 Eichorst SA
Bhuju S 1877 Mussmann M
Bhuju S 1877 Wasmund K
Bhuju S 1877 Herbold CW
Bhuju S 1877 Sedlacek CJ
Ebner F 1878 Horn M
Ebner F 1878 Daims H
Ebner F 1878 Berry D
Ebner F 1878 Loy A
Ebner F 1878 Wagner M
Ebner F 1878 Eichorst SA
Ebner F 1878 Mussmann M
Ebner F 1878 Wasmund K
Ebner F 1878 Herbold CW
Ebner F 1878 Sedlacek CJ
Sedlyarov V 1879 Horn M
Sedlyarov V 1879 Daims H
Sedlyarov V 1879 Berry D
Sedlyarov V 1879 Loy A
Sedlyarov V 1879 Wagner M
Sedlyarov V 1879 Eichorst SA
Sedlyarov V 1879 Mussmann M
Sedlyarov V 1879 Wasmund K
Sedlyarov V 1879 Herbold CW
Sedlyarov V 1879 Sedlacek CJ
Evstatiev R 1880 Horn M
Evstatiev R 1880 Daims H
Evstatiev R 1880 Berry D
Evstatiev R 1880 Loy A
Evstatiev R 1880 Wagner M
Evstatiev R 1880 Eichorst SA
Evstatiev R 1880 Mussmann M
Evstatiev R 1880 Wasmund K
Evstatiev R 1880 Herbold CW
Evstatiev R 1880 Sedlacek CJ
Dammann K 1881 Horn M
Dammann K 1881 Daims H
Dammann K 1881 Berry D
Dammann K 1881 Loy A
Dammann K 1881 Wagner M
Dammann K 1881 Eichorst SA
Dammann K 1881 Mussmann M
Dammann K 1881 Wasmund K
Dammann K 1881 Herbold CW
Dammann K 1881 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Kuzyk O 323 Horn M
Kuzyk O 323 Daims H
Kuzyk O 323 Berry D
Kuzyk O 323 Loy A
Kuzyk O 323 Wagner M
Kuzyk O 323 Eichorst SA
Kuzyk O 323 Mussmann M
Kuzyk O 323 Wasmund K
Kuzyk O 323 Herbold CW
Kuzyk O 323 Sedlacek CJ
Kovarik P 1882 Horn M
Kovarik P 1882 Daims H
Kovarik P 1882 Berry D
Kovarik P 1882 Loy A
Kovarik P 1882 Wagner M
Kovarik P 1882 Eichorst SA
Kovarik P 1882 Mussmann M
Kovarik P 1882 Wasmund K
Kovarik P 1882 Herbold CW
Kovarik P 1882 Sedlacek CJ
Khare V 1883 Horn M
Khare V 1883 Daims H
Khare V 1883 Berry D
Khare V 1883 Loy A
Khare V 1883 Wagner M
Khare V 1883 Eichorst SA
Khare V 1883 Mussmann M
Khare V 1883 Wasmund K
Khare V 1883 Herbold CW
Khare V 1883 Sedlacek CJ
Beibel M 1884 Horn M
Beibel M 1884 Daims H
Beibel M 1884 Berry D
Beibel M 1884 Loy A
Beibel M 1884 Wagner M
Beibel M 1884 Eichorst SA
Beibel M 1884 Mussmann M
Beibel M 1884 Wasmund K
Beibel M 1884 Herbold CW
Beibel M 1884 Sedlacek CJ
Roma G 1885 Horn M
Roma G 1885 Daims H
Roma G 1885 Berry D
Roma G 1885 Loy A
Roma G 1885 Wagner M
Roma G 1885 Eichorst SA
Roma G 1885 Mussmann M
Roma G 1885 Wasmund K
Roma G 1885 Herbold CW
Roma G 1885 Sedlacek CJ
Meisner-Kober N 1886 Horn M
Meisner-Kober N 1886 Daims H
Meisner-Kober N 1886 Berry D
Meisner-Kober N 1886 Loy A
Meisner-Kober N 1886 Wagner M
Meisner-Kober N 1886 Eichorst SA
Meisner-Kober N 1886 Mussmann M
Meisner-Kober N 1886 Wasmund K
Meisner-Kober N 1886 Herbold CW
Meisner-Kober N 1886 Sedlacek CJ
Gasche C 1888 Horn M
Gasche C 1888 Daims H
Gasche C 1888 Berry D
Gasche C 1888 Loy A
Gasche C 1888 Wagner M
Gasche C 1888 Eichorst SA
Gasche C 1888 Mussmann M
Gasche C 1888 Wasmund K
Gasche C 1888 Herbold CW
Gasche C 1888 Sedlacek CJ
Schulz F 296 Horn M
Schulz F 296 Daims H
Schulz F 296 Berry D
Schulz F 296 Loy A
Schulz F 296 Wagner M
Schulz F 296 Eichorst SA
Schulz F 296 Mussmann M
Schulz F 296 Wasmund K
Schulz F 296 Herbold CW
Schulz F 296 Sedlacek CJ
Yutin N 1873 Horn M
Yutin N 1873 Daims H
Yutin N 1873 Berry D
Yutin N 1873 Loy A
Yutin N 1873 Wagner M
Yutin N 1873 Eichorst SA
Yutin N 1873 Mussmann M
Yutin N 1873 Wasmund K
Yutin N 1873 Herbold CW
Yutin N 1873 Sedlacek CJ
Ivanova NN 1870 Horn M
Ivanova NN 1870 Daims H
Ivanova NN 1870 Berry D
Ivanova NN 1870 Loy A
Ivanova NN 1870 Wagner M
Ivanova NN 1870 Eichorst SA
Ivanova NN 1870 Mussmann M
Ivanova NN 1870 Wasmund K
Ivanova NN 1870 Herbold CW
Ivanova NN 1870 Sedlacek CJ
Ortega DR 1871 Horn M
Ortega DR 1871 Daims H
Ortega DR 1871 Berry D
Ortega DR 1871 Loy A
Ortega DR 1871 Wagner M
Ortega DR 1871 Eichorst SA
Ortega DR 1871 Mussmann M
Ortega DR 1871 Wasmund K
Ortega DR 1871 Herbold CW
Ortega DR 1871 Sedlacek CJ
Lee TK 277 Horn M
Lee TK 277 Daims H
Lee TK 277 Berry D
Lee TK 277 Loy A
Lee TK 277 Wagner M
Lee TK 277 Eichorst SA
Lee TK 277 Mussmann M
Lee TK 277 Wasmund K
Lee TK 277 Herbold CW
Lee TK 277 Sedlacek CJ
Vierheilig J 1298 Horn M
Vierheilig J 1298 Daims H
Vierheilig J 1298 Berry D
Vierheilig J 1298 Loy A
Vierheilig J 1298 Wagner M
Vierheilig J 1298 Eichorst SA
Vierheilig J 1298 Mussmann M
Vierheilig J 1298 Wasmund K
Vierheilig J 1298 Herbold CW
Vierheilig J 1298 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Jensen GJ 543 Horn M
Jensen GJ 543 Daims H
Jensen GJ 543 Berry D
Jensen GJ 543 Loy A
Jensen GJ 543 Wagner M
Jensen GJ 543 Eichorst SA
Jensen GJ 543 Mussmann M
Jensen GJ 543 Wasmund K
Jensen GJ 543 Herbold CW
Jensen GJ 543 Sedlacek CJ
Kyrpides NC 613 Horn M
Kyrpides NC 613 Daims H
Kyrpides NC 613 Berry D
Kyrpides NC 613 Loy A
Kyrpides NC 613 Wagner M
Kyrpides NC 613 Eichorst SA
Kyrpides NC 613 Mussmann M
Kyrpides NC 613 Wasmund K
Kyrpides NC 613 Herbold CW
Kyrpides NC 613 Sedlacek CJ
Koonin EV 1872 Horn M
Koonin EV 1872 Daims H
Koonin EV 1872 Berry D
Koonin EV 1872 Loy A
Koonin EV 1872 Wagner M
Koonin EV 1872 Eichorst SA
Koonin EV 1872 Mussmann M
Koonin EV 1872 Wasmund K
Koonin EV 1872 Herbold CW
Koonin EV 1872 Sedlacek CJ
Woyke T 616 Horn M
Woyke T 616 Daims H
Woyke T 616 Berry D
Woyke T 616 Loy A
Woyke T 616 Wagner M
Woyke T 616 Eichorst SA
Woyke T 616 Mussmann M
Woyke T 616 Wasmund K
Woyke T 616 Herbold CW
Woyke T 616 Sedlacek CJ
Zojer M 1860 Horn M
Zojer M 1860 Daims H
Zojer M 1860 Berry D
Zojer M 1860 Loy A
Zojer M 1860 Wagner M
Zojer M 1860 Eichorst SA
Zojer M 1860 Mussmann M
Zojer M 1860 Wasmund K
Zojer M 1860 Herbold CW
Zojer M 1860 Sedlacek CJ
Schuster LN 1861 Horn M
Schuster LN 1861 Daims H
Schuster LN 1861 Berry D
Schuster LN 1861 Loy A
Schuster LN 1861 Wagner M
Schuster LN 1861 Eichorst SA
Schuster LN 1861 Mussmann M
Schuster LN 1861 Wasmund K
Schuster LN 1861 Herbold CW
Schuster LN 1861 Sedlacek CJ
Schulz F 296 Horn M
Schulz F 296 Daims H
Schulz F 296 Berry D
Schulz F 296 Loy A
Schulz F 296 Wagner M
Schulz F 296 Eichorst SA
Schulz F 296 Mussmann M
Schulz F 296 Wasmund K
Schulz F 296 Herbold CW
Schulz F 296 Sedlacek CJ
Pfundner A 1862 Horn M
Pfundner A 1862 Daims H
Pfundner A 1862 Berry D
Pfundner A 1862 Loy A
Pfundner A 1862 Wagner M
Pfundner A 1862 Eichorst SA
Pfundner A 1862 Mussmann M
Pfundner A 1862 Wasmund K
Pfundner A 1862 Herbold CW
Pfundner A 1862 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Rattei T 307 Horn M
Rattei T 307 Daims H
Rattei T 307 Berry D
Rattei T 307 Loy A
Rattei T 307 Wagner M
Rattei T 307 Eichorst SA
Rattei T 307 Mussmann M
Rattei T 307 Wasmund K
Rattei T 307 Herbold CW
Rattei T 307 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Pfann C 1727 Horn M
Pfann C 1727 Daims H
Pfann C 1727 Berry D
Pfann C 1727 Loy A
Pfann C 1727 Wagner M
Pfann C 1727 Eichorst SA
Pfann C 1727 Mussmann M
Pfann C 1727 Wasmund K
Pfann C 1727 Herbold CW
Pfann C 1727 Sedlacek CJ
Steinberger M 1837 Horn M
Steinberger M 1837 Daims H
Steinberger M 1837 Berry D
Steinberger M 1837 Loy A
Steinberger M 1837 Wagner M
Steinberger M 1837 Eichorst SA
Steinberger M 1837 Mussmann M
Steinberger M 1837 Wasmund K
Steinberger M 1837 Herbold CW
Steinberger M 1837 Sedlacek CJ
Hanson B 1425 Horn M
Hanson B 1425 Daims H
Hanson B 1425 Berry D
Hanson B 1425 Loy A
Hanson B 1425 Wagner M
Hanson B 1425 Eichorst SA
Hanson B 1425 Mussmann M
Hanson B 1425 Wasmund K
Hanson B 1425 Herbold CW
Hanson B 1425 Sedlacek CJ
Herp S 1732 Horn M
Herp S 1732 Daims H
Herp S 1732 Berry D
Herp S 1732 Loy A
Herp S 1732 Wagner M
Herp S 1732 Eichorst SA
Herp S 1732 Mussmann M
Herp S 1732 Wasmund K
Herp S 1732 Herbold CW
Herp S 1732 Sedlacek CJ
Brugiroux S 803 Horn M
Brugiroux S 803 Daims H
Brugiroux S 803 Berry D
Brugiroux S 803 Loy A
Brugiroux S 803 Wagner M
Brugiroux S 803 Eichorst SA
Brugiroux S 803 Mussmann M
Brugiroux S 803 Wasmund K
Brugiroux S 803 Herbold CW
Brugiroux S 803 Sedlacek CJ
Gomes Neto JC 1838 Horn M
Gomes Neto JC 1838 Daims H
Gomes Neto JC 1838 Berry D
Gomes Neto JC 1838 Loy A
Gomes Neto JC 1838 Wagner M
Gomes Neto JC 1838 Eichorst SA
Gomes Neto JC 1838 Mussmann M
Gomes Neto JC 1838 Wasmund K
Gomes Neto JC 1838 Herbold CW
Gomes Neto JC 1838 Sedlacek CJ
Boekschoten MV 1839 Horn M
Boekschoten MV 1839 Daims H
Boekschoten MV 1839 Berry D
Boekschoten MV 1839 Loy A
Boekschoten MV 1839 Wagner M
Boekschoten MV 1839 Eichorst SA
Boekschoten MV 1839 Mussmann M
Boekschoten MV 1839 Wasmund K
Boekschoten MV 1839 Herbold CW
Boekschoten MV 1839 Sedlacek CJ
Schwab C 513 Horn M
Schwab C 513 Daims H
Schwab C 513 Berry D
Schwab C 513 Loy A
Schwab C 513 Wagner M
Schwab C 513 Eichorst SA
Schwab C 513 Mussmann M
Schwab C 513 Wasmund K
Schwab C 513 Herbold CW
Schwab C 513 Sedlacek CJ
Urich T 515 Horn M
Urich T 515 Daims H
Urich T 515 Berry D
Urich T 515 Loy A
Urich T 515 Wagner M
Urich T 515 Eichorst SA
Urich T 515 Mussmann M
Urich T 515 Wasmund K
Urich T 515 Herbold CW
Urich T 515 Sedlacek CJ
Ramer-Tait AE 1840 Horn M
Ramer-Tait AE 1840 Daims H
Ramer-Tait AE 1840 Berry D
Ramer-Tait AE 1840 Loy A
Ramer-Tait AE 1840 Wagner M
Ramer-Tait AE 1840 Eichorst SA
Ramer-Tait AE 1840 Mussmann M
Ramer-Tait AE 1840 Wasmund K
Ramer-Tait AE 1840 Herbold CW
Ramer-Tait AE 1840 Sedlacek CJ
Rattei T 307 Horn M
Rattei T 307 Daims H
Rattei T 307 Berry D
Rattei T 307 Loy A
Rattei T 307 Wagner M
Rattei T 307 Eichorst SA
Rattei T 307 Mussmann M
Rattei T 307 Wasmund K
Rattei T 307 Herbold CW
Rattei T 307 Sedlacek CJ
Stecher B 801 Horn M
Stecher B 801 Daims H
Stecher B 801 Berry D
Stecher B 801 Loy A
Stecher B 801 Wagner M
Stecher B 801 Eichorst SA
Stecher B 801 Mussmann M
Stecher B 801 Wasmund K
Stecher B 801 Herbold CW
Stecher B 801 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Szabó G 700 Horn M
Szabó G 700 Daims H
Szabó G 700 Berry D
Szabó G 700 Loy A
Szabó G 700 Wagner M
Szabó G 700 Eichorst SA
Szabó G 700 Mussmann M
Szabó G 700 Wasmund K
Szabó G 700 Herbold CW
Szabó G 700 Sedlacek CJ
Schulz F 296 Horn M
Schulz F 296 Daims H
Schulz F 296 Berry D
Schulz F 296 Loy A
Schulz F 296 Wagner M
Schulz F 296 Eichorst SA
Schulz F 296 Mussmann M
Schulz F 296 Wasmund K
Schulz F 296 Herbold CW
Schulz F 296 Sedlacek CJ
Toenshoff ER 295 Horn M
Toenshoff ER 295 Daims H
Toenshoff ER 295 Berry D
Toenshoff ER 295 Loy A
Toenshoff ER 295 Wagner M
Toenshoff ER 295 Eichorst SA
Toenshoff ER 295 Mussmann M
Toenshoff ER 295 Wasmund K
Toenshoff ER 295 Herbold CW
Toenshoff ER 295 Sedlacek CJ
Volland JM 661 Horn M
Volland JM 661 Daims H
Volland JM 661 Berry D
Volland JM 661 Loy A
Volland JM 661 Wagner M
Volland JM 661 Eichorst SA
Volland JM 661 Mussmann M
Volland JM 661 Wasmund K
Volland JM 661 Herbold CW
Volland JM 661 Sedlacek CJ
Finkel OM 1709 Horn M
Finkel OM 1709 Daims H
Finkel OM 1709 Berry D
Finkel OM 1709 Loy A
Finkel OM 1709 Wagner M
Finkel OM 1709 Eichorst SA
Finkel OM 1709 Mussmann M
Finkel OM 1709 Wasmund K
Finkel OM 1709 Herbold CW
Finkel OM 1709 Sedlacek CJ
Belkin S 1710 Horn M
Belkin S 1710 Daims H
Belkin S 1710 Berry D
Belkin S 1710 Loy A
Belkin S 1710 Wagner M
Belkin S 1710 Eichorst SA
Belkin S 1710 Mussmann M
Belkin S 1710 Wasmund K
Belkin S 1710 Herbold CW
Belkin S 1710 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Manzano-Marín A 1744 Horn M
Manzano-Marín A 1744 Daims H
Manzano-Marín A 1744 Berry D
Manzano-Marín A 1744 Loy A
Manzano-Marín A 1744 Wagner M
Manzano-Marín A 1744 Eichorst SA
Manzano-Marín A 1744 Mussmann M
Manzano-Marín A 1744 Wasmund K
Manzano-Marín A 1744 Herbold CW
Manzano-Marín A 1744 Sedlacek CJ
Szabó G 700 Horn M
Szabó G 700 Daims H
Szabó G 700 Berry D
Szabó G 700 Loy A
Szabó G 700 Wagner M
Szabó G 700 Eichorst SA
Szabó G 700 Mussmann M
Szabó G 700 Wasmund K
Szabó G 700 Herbold CW
Szabó G 700 Sedlacek CJ
Simon JC 1745 Horn M
Simon JC 1745 Daims H
Simon JC 1745 Berry D
Simon JC 1745 Loy A
Simon JC 1745 Wagner M
Simon JC 1745 Eichorst SA
Simon JC 1745 Mussmann M
Simon JC 1745 Wasmund K
Simon JC 1745 Herbold CW
Simon JC 1745 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Latorre A 1746 Horn M
Latorre A 1746 Daims H
Latorre A 1746 Berry D
Latorre A 1746 Loy A
Latorre A 1746 Wagner M
Latorre A 1746 Eichorst SA
Latorre A 1746 Mussmann M
Latorre A 1746 Wasmund K
Latorre A 1746 Herbold CW
Latorre A 1746 Sedlacek CJ
Ponnudurai R 1356 Horn M
Ponnudurai R 1356 Daims H
Ponnudurai R 1356 Berry D
Ponnudurai R 1356 Loy A
Ponnudurai R 1356 Wagner M
Ponnudurai R 1356 Eichorst SA
Ponnudurai R 1356 Mussmann M
Ponnudurai R 1356 Wasmund K
Ponnudurai R 1356 Herbold CW
Ponnudurai R 1356 Sedlacek CJ
Kleiner M 1338 Horn M
Kleiner M 1338 Daims H
Kleiner M 1338 Berry D
Kleiner M 1338 Loy A
Kleiner M 1338 Wagner M
Kleiner M 1338 Eichorst SA
Kleiner M 1338 Mussmann M
Kleiner M 1338 Wasmund K
Kleiner M 1338 Herbold CW
Kleiner M 1338 Sedlacek CJ
Sayavedra L 1355 Horn M
Sayavedra L 1355 Daims H
Sayavedra L 1355 Berry D
Sayavedra L 1355 Loy A
Sayavedra L 1355 Wagner M
Sayavedra L 1355 Eichorst SA
Sayavedra L 1355 Mussmann M
Sayavedra L 1355 Wasmund K
Sayavedra L 1355 Herbold CW
Sayavedra L 1355 Sedlacek CJ
Petersen JM 1317 Horn M
Petersen JM 1317 Daims H
Petersen JM 1317 Berry D
Petersen JM 1317 Loy A
Petersen JM 1317 Wagner M
Petersen JM 1317 Eichorst SA
Petersen JM 1317 Mussmann M
Petersen JM 1317 Wasmund K
Petersen JM 1317 Herbold CW
Petersen JM 1317 Sedlacek CJ
Moche M 1720 Horn M
Moche M 1720 Daims H
Moche M 1720 Berry D
Moche M 1720 Loy A
Moche M 1720 Wagner M
Moche M 1720 Eichorst SA
Moche M 1720 Mussmann M
Moche M 1720 Wasmund K
Moche M 1720 Herbold CW
Moche M 1720 Sedlacek CJ
Otto A 1721 Horn M
Otto A 1721 Daims H
Otto A 1721 Berry D
Otto A 1721 Loy A
Otto A 1721 Wagner M
Otto A 1721 Eichorst SA
Otto A 1721 Mussmann M
Otto A 1721 Wasmund K
Otto A 1721 Herbold CW
Otto A 1721 Sedlacek CJ
Becher D 1364 Horn M
Becher D 1364 Daims H
Becher D 1364 Berry D
Becher D 1364 Loy A
Becher D 1364 Wagner M
Becher D 1364 Eichorst SA
Becher D 1364 Mussmann M
Becher D 1364 Wasmund K
Becher D 1364 Herbold CW
Becher D 1364 Sedlacek CJ
Takeuchi T 1722 Horn M
Takeuchi T 1722 Daims H
Takeuchi T 1722 Berry D
Takeuchi T 1722 Loy A
Takeuchi T 1722 Wagner M
Takeuchi T 1722 Eichorst SA
Takeuchi T 1722 Mussmann M
Takeuchi T 1722 Wasmund K
Takeuchi T 1722 Herbold CW
Takeuchi T 1722 Sedlacek CJ
Satoh N 1358 Horn M
Satoh N 1358 Daims H
Satoh N 1358 Berry D
Satoh N 1358 Loy A
Satoh N 1358 Wagner M
Satoh N 1358 Eichorst SA
Satoh N 1358 Mussmann M
Satoh N 1358 Wasmund K
Satoh N 1358 Herbold CW
Satoh N 1358 Sedlacek CJ
Dubilier N 900 Horn M
Dubilier N 900 Daims H
Dubilier N 900 Berry D
Dubilier N 900 Loy A
Dubilier N 900 Wagner M
Dubilier N 900 Eichorst SA
Dubilier N 900 Mussmann M
Dubilier N 900 Wasmund K
Dubilier N 900 Herbold CW
Dubilier N 900 Sedlacek CJ
Schweder T 1365 Horn M
Schweder T 1365 Daims H
Schweder T 1365 Berry D
Schweder T 1365 Loy A
Schweder T 1365 Wagner M
Schweder T 1365 Eichorst SA
Schweder T 1365 Mussmann M
Schweder T 1365 Wasmund K
Schweder T 1365 Herbold CW
Schweder T 1365 Sedlacek CJ
Markert S 1366 Horn M
Markert S 1366 Daims H
Markert S 1366 Berry D
Markert S 1366 Loy A
Markert S 1366 Wagner M
Markert S 1366 Eichorst SA
Markert S 1366 Mussmann M
Markert S 1366 Wasmund K
Markert S 1366 Herbold CW
Markert S 1366 Sedlacek CJ
Publications | Microbial Ecology, University of Vienna

Publications

Publications in peer reviewed journals

12 Publications found
  • Identification of secondary metabolite gene clusters in the Pseudovibrio genus reveals encouraging biosynthetic potential toward the production of novel bioactive compounds

    Naughton LM, Romano S, O'Gara F, Dobson ADW
    2017 - Front Microbiol, 8: 1494

    Abstract: 

    Increased incidences of antimicrobial resistance and the emergence of pan-resistant 'superbugs' have provoked an extreme sense of urgency amongst researchers focusing on the discovery of potentially novel antimicrobial compounds. A strategic shift in focus from the terrestrial to the marine environment has resulted in the discovery of a wide variety of structurally and functionally diverse bioactive compounds from numerous marine sources, including sponges. Bacteria found in close association with sponges and other marine invertebrates have recently gained much attention as potential sources of many of these novel bioactive compounds. Members of the genus Pseudovibrio are one such group of organisms. In this study, we interrogate the genomes of 21 Pseudovibrio strains isolated from a variety of marine sources, for the presence, diversity and distribution of biosynthetic gene clusters (BGCs). We expand on results obtained from antiSMASH analysis to demonstrate the similarity between the Pseudovibrio-related BGCs and those characterized in other bacteria and corroborate our findings with phylogenetic analysis. We assess how domain organization of the most abundant type of BGCs present among the isolates (Non-ribosomal peptide synthetases and Polyketide synthases) may influence the diversity of compounds produced by these organisms and highlight for the first time the potential for novel compound production from this genus of bacteria, using a genome guided approach.

  • In situ architecture, function, and evolution of a contractile injection system.

    Böck D, Medeiros JM, Tsao HF, Penz T, Weiss GL, Aistleitner K, Horn M, Pilhofer M
    2017 - Science, 6352: 713-717

    Abstract: 

    Contractile injection systems mediate bacterial cell-cell interactions by a bacteriophage tail-like structure. In contrast to extracellular systems, the type 6 secretion system (T6SS) is defined by intracellular localization and attachment to the cytoplasmic membrane. Here we used cryo-focused ion beam milling, electron cryotomography, and functional assays to study a T6SS in Amoebophilus asiaticus The in situ architecture revealed three modules, including a contractile sheath-tube, a baseplate, and an anchor. All modules showed conformational changes upon firing. Lateral baseplate interactions coordinated T6SSs in hexagonal arrays. The system mediated interactions with host membranes and may participate in phagosome escape. Evolutionary sequence analyses predicted that T6SSs are more widespread than previously thought. Our insights form the basis for understanding T6SS key concepts and exploring T6SS diversity.

  • Unexpected genomic features in widespread intracellular bacteria: evidence for motility of marine chlamydiae.

    Collingro A, Köstlbacher S, Mussmann M, Stepanauskas R, Hallam SJ, Horn M
    2017 - ISME J, 10: 2334-2344

    Abstract: 

    Chlamydiae are obligate intracellular bacteria comprising important human pathogens and symbionts of protists. Molecular evidence indicates a tremendous diversity of chlamydiae particularly in marine environments, yet our current knowledge is based mainly on terrestrial representatives. Here we provide first insights into the biology of marine chlamydiae representing three divergent clades. Our analysis of single-cell amplified genomes revealed hallmarks of the chlamydial lifestyle, supporting the ancient origin of their characteristic developmental cycle and major virulence mechanisms. Surprisingly, these chlamydial genomes encode a complete flagellar apparatus, a previously unreported feature. We show that flagella are an ancient trait that was subject to differential gene loss among extant chlamydiae. Together with a chemotaxis system, these marine chlamydiae are likely motile, with flagella potentially playing a role during host cell infection. This study broadens our view on chlamydial biology and indicates a largely underestimated potential to adapt to different hosts and environments.

  • 'Candidatus Cochliophilus cryoturris' (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus.

    Tsao HF, Scheikl U, Volland JM, Köhsler M, Bright M, Walochnik J, Horn M
    2017 - Sci Rep, 1: 3394

    Abstract: 

    Free-living amoebae are well known for their role in controlling microbial community composition through grazing, but some groups, namely Acanthamoeba species, also frequently serve as hosts for bacterial symbionts. Here we report the first identification of a bacterial symbiont in the testate amoeba Cochliopodium. The amoeba was isolated from a cooling tower water sample and identified as C. minus. Fluorescence in situ hybridization and transmission electron microscopy revealed intracellular symbionts located in vacuoles. 16S rRNA-based phylogenetic analysis identified the endosymbiont as member of a monophyletic group within the family Coxiellaceae (Gammaprotebacteria; Legionellales), only moderately related to known amoeba symbionts. We propose to tentatively classify these bacteria as 'Candidatus Cochliophilus cryoturris'. Our findings add both, a novel group of amoeba and a novel group of symbionts, to the growing list of bacteria-amoeba relationships.

  • Biphasic Metabolism and Host Interaction of a Chlamydial Symbiont.

    König L, Siegl A, Penz T, Haider S, Wentrup C, Polzin J, Mann E, Schmitz-Esser S, Domman D, Horn M
    2017 - mSystems, 2: e00202-16

    Abstract: 

    Chlamydiae are obligate intracellular bacteria comprising well-known human pathogens and ubiquitous symbionts of protists, which are characterized by a unique developmental cycle. Here we comprehensively analyzed gene expression dynamics of Protochlamydia amoebophila during infection of its Acanthamoeba host by RNA sequencing. This revealed a highly dynamic transcriptional landscape, where major transcriptional shifts are conserved among chlamydial symbionts and pathogens. Our data served to propose a time-resolved model for type III protein secretion during the developmental cycle, and we provide evidence for a biphasic metabolism of P. amoebophila during infection, which involves energy parasitism and amino acids as the carbon source during initial stages and a postreplicative switch to endogenous glucose-based ATP production. This fits well with major transcriptional changes in the amoeba host, where upregulation of complex sugar breakdown precedes the P. amoebophila metabolic switch. The biphasic chlamydial metabolism represents a unique adaptation to exploit eukaryotic host cells, which likely contributed to the evolutionary success of this group of microbes. IMPORTANCE Chlamydiae are known as major bacterial pathogens of humans, causing the ancient disease trachoma, but they are also frequently found in the environment where they infect ubiquitous protists such as amoebae. All known chlamydiae require a eukaryotic host cell to thrive. Using the environmental chlamydia Protochlamydia amoebophila within its natural host, Acanthamoeba castellanii, we investigated gene expression dynamics in vivo and throughout the complete chlamydial developmental cycle for the first time. This allowed us to infer how a major virulence mechanism, the type III secretion system, is regulated and employed, and we show that the physiology of chlamydiae undergoes a complete shift regarding carbon metabolism and energy generation. This study provides comprehensive insights into the infection strategy of chlamydiae and reveals a unique adaptation to life within a eukaryotic host cell.

  • HuR small-molecule inhibitor elicits differential effects in adenomatosis polyposis and colorectal carcinogenesis

    Lang M, Berry D, Passecker K, Mesteri I, Bhuju S, Ebner F, Sedlyarov V, Evstatiev R, Dammann K, Loy A, Kuzyk O, Kovarik P, Khare V, Beibel M, Roma G, Meisner-Kober N, Gasche C
    2017 - Cancer Res., 77: 2424-2438

    Abstract: 

    HuR is an RNA-binding protein implicated in immune homeostasis and various cancers, including colorectal cancer. HuR binding to AU-rich elements within the 3' untranslated region of mRNAs encoding oncogenes, growth factors, and various cytokines leads message stability and translation. In this study, we evaluated HuR as a small-molecule target for preventing colorectal cancer in high-risk groups such as those with familial adenomatosis polyposis (FAP) or inflammatory bowel disease (IBD). In human specimens, levels of cytoplasmic HuR were increased in colonic epithelial cells from patients with IBD, IBD-cancer, FAP-adenoma, and colorectal cancer, but not in patients with IBD-dysplasia. Intraperitoneal injection of the HuR small-molecule inhibitor MS-444 in AOM/DSS mice, a model of IBD and inflammatory colon cancer, augmented DSS-induced weight loss and increased tumor multiplicity, size, and invasiveness. MS-444 treatment also abrogated tumor cell apoptosis and depleted tumor-associated eosinophils, accompanied by a decrease in IL18 and eotaxin-1. In contrast, HuR inhibition in APCMin mice, a model of FAP and colon cancer, diminished the number of small intestinal tumors generated. In this setting, fecal microbiota, evaluated by 16S rRNA gene amplicon sequencing, shifted to a state of reduced bacterial diversity, with an increased representation of Prevotella, Akkermansia, and Lachnospiraceae Taken together, our results indicate that HuR activation is an early event in FAP-adenoma but is not present in IBD-dysplasia. Furthermore, our results offer a preclinical proof of concept for HuR inhibition as an effective means of FAP chemoprevention, with caution advised in the setting of IBD.

  • Giant viruses with an expanded complement of translation system components.

    Schulz F, Yutin N, Ivanova NN, Ortega DR, Lee TK, Vierheilig J, Daims H, Horn M, Wagner M, Jensen GJ, Kyrpides NC, Koonin EV, Woyke T
    2017 - Science, 6333: 82-85
    Klosneuvirus

    Abstract: 

    The discovery of giant viruses blurred the sharp division between viruses and cellular life. Giant virus genomes encode proteins considered as signatures of cellular organisms, particularly translation system components, prompting hypotheses that these viruses derived from a fourth domain of cellular life. Here we report the discovery of a group of giant viruses (Klosneuviruses) in metagenomic data. Compared with other giant viruses, the Klosneuviruses encode an expanded translation machinery, including aminoacyl transfer RNA synthetases with specificities for all 20 amino acids. Notwithstanding the prevalence of translation system components, comprehensive phylogenomic analysis of these genes indicates that Klosneuviruses did not evolve from a cellular ancestor but rather are derived from a much smaller virus through extensive gain of host genes.

  • Variant profiling of evolving prokaryotic populations.

    Zojer M, Schuster LN, Schulz F, Pfundner A, Horn M, Rattei T
    2017 - PeerJ, e2997

    Abstract: 

    Genomic heterogeneity of bacterial species is observed and studied in experimental evolution experiments and clinical diagnostics, and occurs as micro-diversity of natural habitats. The challenge for genome research is to accurately capture this heterogeneity with the currently used short sequencing reads. Recent advances in NGS technologies improved the speed and coverage and thus allowed for deep sequencing of bacterial populations. This facilitates the quantitative assessment of genomic heterogeneity, including low frequency alleles or haplotypes. However, false positive variant predictions due to sequencing errors and mapping artifacts of short reads need to be prevented. We therefore created VarCap, a workflow for the reliable prediction of different types of variants even at low frequencies. In order to predict SNPs, InDels and structural variations, we evaluated the sensitivity and accuracy of different software tools using synthetic read data. The results suggested that the best sensitivity could be reached by a union of different tools, however at the price of increased false positives. We identified possible reasons for false predictions and used this knowledge to improve the accuracy by post-filtering the predicted variants according to properties such as frequency, coverage, genomic environment/localization and co-localization with other variants. We observed that best precision was achieved by using an intersection of at least two tools per variant. This resulted in the reliable prediction of variants above a minimum relative abundance of 2%. VarCap is designed for being routinely used within experimental evolution experiments or for clinical diagnostics. The detected variants are reported as frequencies within a VCF file and as a graphical overview of the distribution of the different variant/allele/haplotype frequencies. The source code of VarCap is available at https://github.com/ma2o/VarCap. In order to provide this workflow to a broad community, we implemeted VarCap on a Galaxy webserver, which is accessible at http://galaxy.csb.univie.ac.at.

  • Lifestyle and horizontal gene transfer-mediated evolution of Mucispirillum schaedleri, a core member of the murine gut microbiota

    Loy A, Pfann C, Steinberger M, Hanson B, Herp S, Brugiroux S, Gomes Neto JC, Boekschoten MV, Schwab C, Urich T, Ramer-Tait AE, Rattei T, Stecher B, Berry D
    2017 - mSystems, 2: e00171-16

    Abstract: 

    Mucispirillum schaedleri is an abundant inhabitant of the intestinal mucus layer of rodents and other animals and has been suggested to be a pathobiont, a commensal that plays a role in disease. In order to gain insights into its lifestyle, we analyzed the genome and transcriptome of M. schaedleri ASF 457 and performed physiological experiments to test traits predicted by its genome. Although described as a mucus inhabitant, M. schaedleri has limited capacity for degrading host-derived mucosal glycans and other complex polysaccharides. Additionally, M. schaedleri reduces nitrate and expresses systems for scavenging oxygen and reactive oxygen species in vivo, which may account for its localization close to the mucosal tissue and expansion during inflammation. Also of note, M. schaedleri harbors a type VI secretion system and putative effector proteins and can modify gene expression in mucosal tissue, suggesting intimate interactions with its host and a possible role in inflammation. The M. schaedleri genome has been shaped by extensive horizontal gene transfer, primarily from intestinal Epsilon- and Deltaproteobacteria, indicating that horizontal gene transfer has played a key role in defining its niche in the gut ecosystem.

  • Convergent patterns in the evolution of mealybug symbioses involving different intrabacterial symbionts.

    Szabó G, Schulz F, Toenshoff ER, Volland JM, Finkel OM, Belkin S, Horn M
    2017 - ISME J, 3: 715-726
    Mealybugs (Trabutina mannipara) enveloped in a wax cover feeding on Tamarix twig

    Abstract: 

    Mealybugs (Insecta: Hemiptera: Pseudococcidae) maintain obligatory relationships with bacterial symbionts, which provide essential nutrients to their insect hosts. Most pseudococcinae mealybugs harbor a unique symbiosis setup with enlarged betaproteobacterial symbionts ('Candidatus Tremblaya princeps'), which themselves contain gammaproteobacterial symbionts. Here we investigated the symbiosis of the manna mealybug, Trabutina mannipara, using a metagenomic approach. Phylogenetic analyses revealed that the intrabacterial symbiont of T. mannipara represents a novel lineage within the Gammaproteobacteria, for which we propose the tentative name 'Candidatus Trabutinella endobia'. Combining our results with previous data available for the nested symbiosis of the citrus mealybug Planococcus citri, we show that synthesis of essential amino acids and vitamins and translation-related functions partition between the symbiotic partners in a highly similar manner in the two systems, despite the distinct evolutionary origin of the intrabacterial symbionts. Bacterial genes found in both mealybug genomes and complementing missing functions in both symbioses were likely integrated in ancestral mealybugs before T. mannipara and P. citri diversified. The high level of correspondence between the two mealybug systems and their highly intertwined metabolic pathways are unprecedented. Our work contributes to a better understanding of the only known intracellular symbiosis between two bacteria and suggests that the evolution of this unique symbiosis included the replacement of intrabacterial symbionts in ancestral mealybugs.

  • Happens in the best of subfamilies: establishment and repeated replacements of co-obligate secondary endosymbionts within Lachninae aphids.

    Manzano-Marín A, Szabó G, Simon JC, Horn M, Latorre A
    2017 - Environ. Microbiol., 1: 393-408

    Abstract: 

    Virtually all aphids maintain an obligate mutualistic symbiosis with bacteria from the Buchnera genus, which produce essential nutrients for their aphid hosts. Most aphids from the Lachninae subfamily have been consistently found to house additional endosymbionts, mainly Serratia symbiotica. This apparent dependence on secondary endosymbionts was proposed to have been triggered by the loss of the riboflavin biosynthetic capability by Buchnera in the Lachninae last common ancestor. However, an integral large-scale analysis of secondary endosymbionts in the Lachninae is still missing, hampering the interpretation of the evolutionary and genomic analyses of these endosymbionts. Here, we analysed the endosymbionts of selected representatives from seven different Lachninae genera and nineteen species, spanning four tribes, both by FISH (exploring the symbionts' morphology and tissue tropism) and 16S rRNA gene sequencing. We demonstrate that all analysed aphids possess dual symbiotic systems, and while most harbour S. symbiotica, some have undergone symbiont replacement by other phylogenetically-distinct bacterial taxa. We found that these secondary associates display contrasting cell shapes and tissue tropism, and some appear to be lineage-specific. We propose a scenario for symbiont establishment in the Lachninae, followed by changes in the symbiont's tissue tropism and symbiont replacement events, thereby highlighting the extraordinary versatility of host-symbiont interactions.

  • Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis.

    Ponnudurai R, Kleiner M, Sayavedra L, Petersen JM, Moche M, Otto A, Becher D, Takeuchi T, Satoh N, Dubilier N, Schweder T, Markert S
    2017 - ISME J, 11: 463–477

    Abstract: 

    The hydrothermal vent mussel Bathymodiolus azoricus lives in an intimate symbiosis with two types of chemosynthetic Gammaproteobacteria in its gills: a sulfur oxidizer and a methane oxidizer. Despite numerous investigations over the last decades, the degree of interdependence between the three symbiotic partners, their individual metabolic contributions, as well as the mechanism of carbon transfer from the symbionts to the host are poorly understood. We used a combination of proteomics and genomics to investigate the physiology and metabolism of the individual symbiotic partners. Our study revealed that key metabolic functions are most likely accomplished jointly by B. azoricus and its symbionts: (1) CO2 is pre-concentrated by the host for carbon fixation by the sulfur-oxidizing symbiont, and (2) the host replenishes essential biosynthetic TCA cycle intermediates for the sulfur-oxidizing symbiont. In return (3), the sulfur oxidizer may compensate for the host's putative deficiency in amino acid and cofactor biosynthesis. We also identified numerous 'symbiosis-specific' host proteins by comparing symbiont-containing and symbiont-free host tissues and symbiont fractions. These proteins included a large complement of host digestive enzymes in the gill that are likely involved in symbiont digestion and carbon transfer from the symbionts to the host.The ISME Journal advance online publication, 1 November 2016; doi:10.1038/ismej.2016.124.

Book chapters and other publications

1 Publication found
  • Principles of Systems Biology, No. 13 - A pathogen-resistant designer microbiota

    Stecher B, Clavel T, Loy A, Berry D
    2017 - Cell Systems, 4: 3-6