Eichorst SA 650 Horn M
Eichorst SA 650 Daims H
Eichorst SA 650 Berry D
Eichorst SA 650 Loy A
Eichorst SA 650 Wagner M
Eichorst SA 650 Eichorst SA
Eichorst SA 650 Mussmann M
Eichorst SA 650 Wasmund K
Eichorst SA 650 Herbold CW
Eichorst SA 650 Sedlacek CJ
Trojan D 1896 Horn M
Trojan D 1896 Daims H
Trojan D 1896 Berry D
Trojan D 1896 Loy A
Trojan D 1896 Wagner M
Trojan D 1896 Eichorst SA
Trojan D 1896 Mussmann M
Trojan D 1896 Wasmund K
Trojan D 1896 Herbold CW
Trojan D 1896 Sedlacek CJ
Woebken D 278 Horn M
Woebken D 278 Daims H
Woebken D 278 Berry D
Woebken D 278 Loy A
Woebken D 278 Wagner M
Woebken D 278 Eichorst SA
Woebken D 278 Mussmann M
Woebken D 278 Wasmund K
Woebken D 278 Herbold CW
Woebken D 278 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Stecher B 801 Horn M
Stecher B 801 Daims H
Stecher B 801 Berry D
Stecher B 801 Loy A
Stecher B 801 Wagner M
Stecher B 801 Eichorst SA
Stecher B 801 Mussmann M
Stecher B 801 Wasmund K
Stecher B 801 Herbold CW
Stecher B 801 Sedlacek CJ
Clavel T 728 Horn M
Clavel T 728 Daims H
Clavel T 728 Berry D
Clavel T 728 Loy A
Clavel T 728 Wagner M
Clavel T 728 Eichorst SA
Clavel T 728 Mussmann M
Clavel T 728 Wasmund K
Clavel T 728 Herbold CW
Clavel T 728 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Harrison JP 1767 Horn M
Harrison JP 1767 Daims H
Harrison JP 1767 Berry D
Harrison JP 1767 Loy A
Harrison JP 1767 Wagner M
Harrison JP 1767 Eichorst SA
Harrison JP 1767 Mussmann M
Harrison JP 1767 Wasmund K
Harrison JP 1767 Herbold CW
Harrison JP 1767 Sedlacek CJ
Angel R 325 Horn M
Angel R 325 Daims H
Angel R 325 Berry D
Angel R 325 Loy A
Angel R 325 Wagner M
Angel R 325 Eichorst SA
Angel R 325 Mussmann M
Angel R 325 Wasmund K
Angel R 325 Herbold CW
Angel R 325 Sedlacek CJ
Cockell CS 1781 Horn M
Cockell CS 1781 Daims H
Cockell CS 1781 Berry D
Cockell CS 1781 Loy A
Cockell CS 1781 Wagner M
Cockell CS 1781 Eichorst SA
Cockell CS 1781 Mussmann M
Cockell CS 1781 Wasmund K
Cockell CS 1781 Herbold CW
Cockell CS 1781 Sedlacek CJ
Füssel J 1982 Horn M
Füssel J 1982 Daims H
Füssel J 1982 Berry D
Füssel J 1982 Loy A
Füssel J 1982 Wagner M
Füssel J 1982 Eichorst SA
Füssel J 1982 Mussmann M
Füssel J 1982 Wasmund K
Füssel J 1982 Herbold CW
Füssel J 1982 Sedlacek CJ
Lücker S 336 Horn M
Lücker S 336 Daims H
Lücker S 336 Berry D
Lücker S 336 Loy A
Lücker S 336 Wagner M
Lücker S 336 Eichorst SA
Lücker S 336 Mussmann M
Lücker S 336 Wasmund K
Lücker S 336 Herbold CW
Lücker S 336 Sedlacek CJ
Yilmaz P 1983 Horn M
Yilmaz P 1983 Daims H
Yilmaz P 1983 Berry D
Yilmaz P 1983 Loy A
Yilmaz P 1983 Wagner M
Yilmaz P 1983 Eichorst SA
Yilmaz P 1983 Mussmann M
Yilmaz P 1983 Wasmund K
Yilmaz P 1983 Herbold CW
Yilmaz P 1983 Sedlacek CJ
Nowka B 363 Horn M
Nowka B 363 Daims H
Nowka B 363 Berry D
Nowka B 363 Loy A
Nowka B 363 Wagner M
Nowka B 363 Eichorst SA
Nowka B 363 Mussmann M
Nowka B 363 Wasmund K
Nowka B 363 Herbold CW
Nowka B 363 Sedlacek CJ
van Kessel MAHJ 1899 Horn M
van Kessel MAHJ 1899 Daims H
van Kessel MAHJ 1899 Berry D
van Kessel MAHJ 1899 Loy A
van Kessel MAHJ 1899 Wagner M
van Kessel MAHJ 1899 Eichorst SA
van Kessel MAHJ 1899 Mussmann M
van Kessel MAHJ 1899 Wasmund K
van Kessel MAHJ 1899 Herbold CW
van Kessel MAHJ 1899 Sedlacek CJ
Bourceau P 1984 Horn M
Bourceau P 1984 Daims H
Bourceau P 1984 Berry D
Bourceau P 1984 Loy A
Bourceau P 1984 Wagner M
Bourceau P 1984 Eichorst SA
Bourceau P 1984 Mussmann M
Bourceau P 1984 Wasmund K
Bourceau P 1984 Herbold CW
Bourceau P 1984 Sedlacek CJ
Hach PF 1754 Horn M
Hach PF 1754 Daims H
Hach PF 1754 Berry D
Hach PF 1754 Loy A
Hach PF 1754 Wagner M
Hach PF 1754 Eichorst SA
Hach PF 1754 Mussmann M
Hach PF 1754 Wasmund K
Hach PF 1754 Herbold CW
Hach PF 1754 Sedlacek CJ
Littmann S 1902 Horn M
Littmann S 1902 Daims H
Littmann S 1902 Berry D
Littmann S 1902 Loy A
Littmann S 1902 Wagner M
Littmann S 1902 Eichorst SA
Littmann S 1902 Mussmann M
Littmann S 1902 Wasmund K
Littmann S 1902 Herbold CW
Littmann S 1902 Sedlacek CJ
Berg J 1985 Horn M
Berg J 1985 Daims H
Berg J 1985 Berry D
Berg J 1985 Loy A
Berg J 1985 Wagner M
Berg J 1985 Eichorst SA
Berg J 1985 Mussmann M
Berg J 1985 Wasmund K
Berg J 1985 Herbold CW
Berg J 1985 Sedlacek CJ
Spieck E 338 Horn M
Spieck E 338 Daims H
Spieck E 338 Berry D
Spieck E 338 Loy A
Spieck E 338 Wagner M
Spieck E 338 Eichorst SA
Spieck E 338 Mussmann M
Spieck E 338 Wasmund K
Spieck E 338 Herbold CW
Spieck E 338 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Kuypers MMM 1026 Horn M
Kuypers MMM 1026 Daims H
Kuypers MMM 1026 Berry D
Kuypers MMM 1026 Loy A
Kuypers MMM 1026 Wagner M
Kuypers MMM 1026 Eichorst SA
Kuypers MMM 1026 Mussmann M
Kuypers MMM 1026 Wasmund K
Kuypers MMM 1026 Herbold CW
Kuypers MMM 1026 Sedlacek CJ
Lam P 1236 Horn M
Lam P 1236 Daims H
Lam P 1236 Berry D
Lam P 1236 Loy A
Lam P 1236 Wagner M
Lam P 1236 Eichorst SA
Lam P 1236 Mussmann M
Lam P 1236 Wasmund K
Lam P 1236 Herbold CW
Lam P 1236 Sedlacek CJ
Hubalek V 1966 Horn M
Hubalek V 1966 Daims H
Hubalek V 1966 Berry D
Hubalek V 1966 Loy A
Hubalek V 1966 Wagner M
Hubalek V 1966 Eichorst SA
Hubalek V 1966 Mussmann M
Hubalek V 1966 Wasmund K
Hubalek V 1966 Herbold CW
Hubalek V 1966 Sedlacek CJ
Buck M 1967 Horn M
Buck M 1967 Daims H
Buck M 1967 Berry D
Buck M 1967 Loy A
Buck M 1967 Wagner M
Buck M 1967 Eichorst SA
Buck M 1967 Mussmann M
Buck M 1967 Wasmund K
Buck M 1967 Herbold CW
Buck M 1967 Sedlacek CJ
Tan B 1968 Horn M
Tan B 1968 Daims H
Tan B 1968 Berry D
Tan B 1968 Loy A
Tan B 1968 Wagner M
Tan B 1968 Eichorst SA
Tan B 1968 Mussmann M
Tan B 1968 Wasmund K
Tan B 1968 Herbold CW
Tan B 1968 Sedlacek CJ
Foght J 1969 Horn M
Foght J 1969 Daims H
Foght J 1969 Berry D
Foght J 1969 Loy A
Foght J 1969 Wagner M
Foght J 1969 Eichorst SA
Foght J 1969 Mussmann M
Foght J 1969 Wasmund K
Foght J 1969 Herbold CW
Foght J 1969 Sedlacek CJ
Wendeberg A 1970 Horn M
Wendeberg A 1970 Daims H
Wendeberg A 1970 Berry D
Wendeberg A 1970 Loy A
Wendeberg A 1970 Wagner M
Wendeberg A 1970 Eichorst SA
Wendeberg A 1970 Mussmann M
Wendeberg A 1970 Wasmund K
Wendeberg A 1970 Herbold CW
Wendeberg A 1970 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Bertilsson S 1971 Horn M
Bertilsson S 1971 Daims H
Bertilsson S 1971 Berry D
Bertilsson S 1971 Loy A
Bertilsson S 1971 Wagner M
Bertilsson S 1971 Eichorst SA
Bertilsson S 1971 Mussmann M
Bertilsson S 1971 Wasmund K
Bertilsson S 1971 Herbold CW
Bertilsson S 1971 Sedlacek CJ
Eiler A 1972 Horn M
Eiler A 1972 Daims H
Eiler A 1972 Berry D
Eiler A 1972 Loy A
Eiler A 1972 Wagner M
Eiler A 1972 Eichorst SA
Eiler A 1972 Mussmann M
Eiler A 1972 Wasmund K
Eiler A 1972 Herbold CW
Eiler A 1972 Sedlacek CJ
Herbold CW 321 Horn M
Herbold CW 321 Daims H
Herbold CW 321 Berry D
Herbold CW 321 Loy A
Herbold CW 321 Wagner M
Herbold CW 321 Eichorst SA
Herbold CW 321 Mussmann M
Herbold CW 321 Wasmund K
Herbold CW 321 Herbold CW
Herbold CW 321 Sedlacek CJ
Lehtovirta-Morley LE 1935 Horn M
Lehtovirta-Morley LE 1935 Daims H
Lehtovirta-Morley LE 1935 Berry D
Lehtovirta-Morley LE 1935 Loy A
Lehtovirta-Morley LE 1935 Wagner M
Lehtovirta-Morley LE 1935 Eichorst SA
Lehtovirta-Morley LE 1935 Mussmann M
Lehtovirta-Morley LE 1935 Wasmund K
Lehtovirta-Morley LE 1935 Herbold CW
Lehtovirta-Morley LE 1935 Sedlacek CJ
Jung MY 1956 Horn M
Jung MY 1956 Daims H
Jung MY 1956 Berry D
Jung MY 1956 Loy A
Jung MY 1956 Wagner M
Jung MY 1956 Eichorst SA
Jung MY 1956 Mussmann M
Jung MY 1956 Wasmund K
Jung MY 1956 Herbold CW
Jung MY 1956 Sedlacek CJ
Jehmlich N 373 Horn M
Jehmlich N 373 Daims H
Jehmlich N 373 Berry D
Jehmlich N 373 Loy A
Jehmlich N 373 Wagner M
Jehmlich N 373 Eichorst SA
Jehmlich N 373 Mussmann M
Jehmlich N 373 Wasmund K
Jehmlich N 373 Herbold CW
Jehmlich N 373 Sedlacek CJ
Hausmann B 324 Horn M
Hausmann B 324 Daims H
Hausmann B 324 Berry D
Hausmann B 324 Loy A
Hausmann B 324 Wagner M
Hausmann B 324 Eichorst SA
Hausmann B 324 Mussmann M
Hausmann B 324 Wasmund K
Hausmann B 324 Herbold CW
Hausmann B 324 Sedlacek CJ
Han P 375 Horn M
Han P 375 Daims H
Han P 375 Berry D
Han P 375 Loy A
Han P 375 Wagner M
Han P 375 Eichorst SA
Han P 375 Mussmann M
Han P 375 Wasmund K
Han P 375 Herbold CW
Han P 375 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Pester M 303 Horn M
Pester M 303 Daims H
Pester M 303 Berry D
Pester M 303 Loy A
Pester M 303 Wagner M
Pester M 303 Eichorst SA
Pester M 303 Mussmann M
Pester M 303 Wasmund K
Pester M 303 Herbold CW
Pester M 303 Sedlacek CJ
Sayavedra-Soto LA 1937 Horn M
Sayavedra-Soto LA 1937 Daims H
Sayavedra-Soto LA 1937 Berry D
Sayavedra-Soto LA 1937 Loy A
Sayavedra-Soto LA 1937 Wagner M
Sayavedra-Soto LA 1937 Eichorst SA
Sayavedra-Soto LA 1937 Mussmann M
Sayavedra-Soto LA 1937 Wasmund K
Sayavedra-Soto LA 1937 Herbold CW
Sayavedra-Soto LA 1937 Sedlacek CJ
Rhee SK 1957 Horn M
Rhee SK 1957 Daims H
Rhee SK 1957 Berry D
Rhee SK 1957 Loy A
Rhee SK 1957 Wagner M
Rhee SK 1957 Eichorst SA
Rhee SK 1957 Mussmann M
Rhee SK 1957 Wasmund K
Rhee SK 1957 Herbold CW
Rhee SK 1957 Sedlacek CJ
Prosser JI 1939 Horn M
Prosser JI 1939 Daims H
Prosser JI 1939 Berry D
Prosser JI 1939 Loy A
Prosser JI 1939 Wagner M
Prosser JI 1939 Eichorst SA
Prosser JI 1939 Mussmann M
Prosser JI 1939 Wasmund K
Prosser JI 1939 Herbold CW
Prosser JI 1939 Sedlacek CJ
Nicol GW 1940 Horn M
Nicol GW 1940 Daims H
Nicol GW 1940 Berry D
Nicol GW 1940 Loy A
Nicol GW 1940 Wagner M
Nicol GW 1940 Eichorst SA
Nicol GW 1940 Mussmann M
Nicol GW 1940 Wasmund K
Nicol GW 1940 Herbold CW
Nicol GW 1940 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Gubry-Rangin C 1941 Horn M
Gubry-Rangin C 1941 Daims H
Gubry-Rangin C 1941 Berry D
Gubry-Rangin C 1941 Loy A
Gubry-Rangin C 1941 Wagner M
Gubry-Rangin C 1941 Eichorst SA
Gubry-Rangin C 1941 Mussmann M
Gubry-Rangin C 1941 Wasmund K
Gubry-Rangin C 1941 Herbold CW
Gubry-Rangin C 1941 Sedlacek CJ
Liu S 1932 Horn M
Liu S 1932 Daims H
Liu S 1932 Berry D
Liu S 1932 Loy A
Liu S 1932 Wagner M
Liu S 1932 Eichorst SA
Liu S 1932 Mussmann M
Liu S 1932 Wasmund K
Liu S 1932 Herbold CW
Liu S 1932 Sedlacek CJ
Han P 375 Horn M
Han P 375 Daims H
Han P 375 Berry D
Han P 375 Loy A
Han P 375 Wagner M
Han P 375 Eichorst SA
Han P 375 Mussmann M
Han P 375 Wasmund K
Han P 375 Herbold CW
Han P 375 Sedlacek CJ
Hink L 1933 Horn M
Hink L 1933 Daims H
Hink L 1933 Berry D
Hink L 1933 Loy A
Hink L 1933 Wagner M
Hink L 1933 Eichorst SA
Hink L 1933 Mussmann M
Hink L 1933 Wasmund K
Hink L 1933 Herbold CW
Hink L 1933 Sedlacek CJ
Prosser JI 1939 Horn M
Prosser JI 1939 Daims H
Prosser JI 1939 Berry D
Prosser JI 1939 Loy A
Prosser JI 1939 Wagner M
Prosser JI 1939 Eichorst SA
Prosser JI 1939 Mussmann M
Prosser JI 1939 Wasmund K
Prosser JI 1939 Herbold CW
Prosser JI 1939 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Brüggemann N 1934 Horn M
Brüggemann N 1934 Daims H
Brüggemann N 1934 Berry D
Brüggemann N 1934 Loy A
Brüggemann N 1934 Wagner M
Brüggemann N 1934 Eichorst SA
Brüggemann N 1934 Mussmann M
Brüggemann N 1934 Wasmund K
Brüggemann N 1934 Herbold CW
Brüggemann N 1934 Sedlacek CJ
Jochum LM 1916 Horn M
Jochum LM 1916 Daims H
Jochum LM 1916 Berry D
Jochum LM 1916 Loy A
Jochum LM 1916 Wagner M
Jochum LM 1916 Eichorst SA
Jochum LM 1916 Mussmann M
Jochum LM 1916 Wasmund K
Jochum LM 1916 Herbold CW
Jochum LM 1916 Sedlacek CJ
Chena X 1917 Horn M
Chena X 1917 Daims H
Chena X 1917 Berry D
Chena X 1917 Loy A
Chena X 1917 Wagner M
Chena X 1917 Eichorst SA
Chena X 1917 Mussmann M
Chena X 1917 Wasmund K
Chena X 1917 Herbold CW
Chena X 1917 Sedlacek CJ
Lever MA 361 Horn M
Lever MA 361 Daims H
Lever MA 361 Berry D
Lever MA 361 Loy A
Lever MA 361 Wagner M
Lever MA 361 Eichorst SA
Lever MA 361 Mussmann M
Lever MA 361 Wasmund K
Lever MA 361 Herbold CW
Lever MA 361 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Jørgensen BB 362 Horn M
Jørgensen BB 362 Daims H
Jørgensen BB 362 Berry D
Jørgensen BB 362 Loy A
Jørgensen BB 362 Wagner M
Jørgensen BB 362 Eichorst SA
Jørgensen BB 362 Mussmann M
Jørgensen BB 362 Wasmund K
Jørgensen BB 362 Herbold CW
Jørgensen BB 362 Sedlacek CJ
Schramm A 453 Horn M
Schramm A 453 Daims H
Schramm A 453 Berry D
Schramm A 453 Loy A
Schramm A 453 Wagner M
Schramm A 453 Eichorst SA
Schramm A 453 Mussmann M
Schramm A 453 Wasmund K
Schramm A 453 Herbold CW
Schramm A 453 Sedlacek CJ
Kjeldsen KU 359 Horn M
Kjeldsen KU 359 Daims H
Kjeldsen KU 359 Berry D
Kjeldsen KU 359 Loy A
Kjeldsen KU 359 Wagner M
Kjeldsen KU 359 Eichorst SA
Kjeldsen KU 359 Mussmann M
Kjeldsen KU 359 Wasmund K
Kjeldsen KU 359 Herbold CW
Kjeldsen KU 359 Sedlacek CJ
Lesaulnier CC 1920 Horn M
Lesaulnier CC 1920 Daims H
Lesaulnier CC 1920 Berry D
Lesaulnier CC 1920 Loy A
Lesaulnier CC 1920 Wagner M
Lesaulnier CC 1920 Eichorst SA
Lesaulnier CC 1920 Mussmann M
Lesaulnier CC 1920 Wasmund K
Lesaulnier CC 1920 Herbold CW
Lesaulnier CC 1920 Sedlacek CJ
Herbold CW 321 Horn M
Herbold CW 321 Daims H
Herbold CW 321 Berry D
Herbold CW 321 Loy A
Herbold CW 321 Wagner M
Herbold CW 321 Eichorst SA
Herbold CW 321 Mussmann M
Herbold CW 321 Wasmund K
Herbold CW 321 Herbold CW
Herbold CW 321 Sedlacek CJ
Pelikan C 322 Horn M
Pelikan C 322 Daims H
Pelikan C 322 Berry D
Pelikan C 322 Loy A
Pelikan C 322 Wagner M
Pelikan C 322 Eichorst SA
Pelikan C 322 Mussmann M
Pelikan C 322 Wasmund K
Pelikan C 322 Herbold CW
Pelikan C 322 Sedlacek CJ
Gérard C 1921 Horn M
Gérard C 1921 Daims H
Gérard C 1921 Berry D
Gérard C 1921 Loy A
Gérard C 1921 Wagner M
Gérard C 1921 Eichorst SA
Gérard C 1921 Mussmann M
Gérard C 1921 Wasmund K
Gérard C 1921 Herbold CW
Gérard C 1921 Sedlacek CJ
Le Coz X 1922 Horn M
Le Coz X 1922 Daims H
Le Coz X 1922 Berry D
Le Coz X 1922 Loy A
Le Coz X 1922 Wagner M
Le Coz X 1922 Eichorst SA
Le Coz X 1922 Mussmann M
Le Coz X 1922 Wasmund K
Le Coz X 1922 Herbold CW
Le Coz X 1922 Sedlacek CJ
Gagnot S 1923 Horn M
Gagnot S 1923 Daims H
Gagnot S 1923 Berry D
Gagnot S 1923 Loy A
Gagnot S 1923 Wagner M
Gagnot S 1923 Eichorst SA
Gagnot S 1923 Mussmann M
Gagnot S 1923 Wasmund K
Gagnot S 1923 Herbold CW
Gagnot S 1923 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Niggemann J 1924 Horn M
Niggemann J 1924 Daims H
Niggemann J 1924 Berry D
Niggemann J 1924 Loy A
Niggemann J 1924 Wagner M
Niggemann J 1924 Eichorst SA
Niggemann J 1924 Mussmann M
Niggemann J 1924 Wasmund K
Niggemann J 1924 Herbold CW
Niggemann J 1924 Sedlacek CJ
Dittmar T 1925 Horn M
Dittmar T 1925 Daims H
Dittmar T 1925 Berry D
Dittmar T 1925 Loy A
Dittmar T 1925 Wagner M
Dittmar T 1925 Eichorst SA
Dittmar T 1925 Mussmann M
Dittmar T 1925 Wasmund K
Dittmar T 1925 Herbold CW
Dittmar T 1925 Sedlacek CJ
Singer GA 1153 Horn M
Singer GA 1153 Daims H
Singer GA 1153 Berry D
Singer GA 1153 Loy A
Singer GA 1153 Wagner M
Singer GA 1153 Eichorst SA
Singer GA 1153 Mussmann M
Singer GA 1153 Wasmund K
Singer GA 1153 Herbold CW
Singer GA 1153 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Naughton LM 1914 Horn M
Naughton LM 1914 Daims H
Naughton LM 1914 Berry D
Naughton LM 1914 Loy A
Naughton LM 1914 Wagner M
Naughton LM 1914 Eichorst SA
Naughton LM 1914 Mussmann M
Naughton LM 1914 Wasmund K
Naughton LM 1914 Herbold CW
Naughton LM 1914 Sedlacek CJ
Romano S 1910 Horn M
Romano S 1910 Daims H
Romano S 1910 Berry D
Romano S 1910 Loy A
Romano S 1910 Wagner M
Romano S 1910 Eichorst SA
Romano S 1910 Mussmann M
Romano S 1910 Wasmund K
Romano S 1910 Herbold CW
Romano S 1910 Sedlacek CJ
O'Gara F 1469 Horn M
O'Gara F 1469 Daims H
O'Gara F 1469 Berry D
O'Gara F 1469 Loy A
O'Gara F 1469 Wagner M
O'Gara F 1469 Eichorst SA
O'Gara F 1469 Mussmann M
O'Gara F 1469 Wasmund K
O'Gara F 1469 Herbold CW
O'Gara F 1469 Sedlacek CJ
Dobson ADW 1915 Horn M
Dobson ADW 1915 Daims H
Dobson ADW 1915 Berry D
Dobson ADW 1915 Loy A
Dobson ADW 1915 Wagner M
Dobson ADW 1915 Eichorst SA
Dobson ADW 1915 Mussmann M
Dobson ADW 1915 Wasmund K
Dobson ADW 1915 Herbold CW
Dobson ADW 1915 Sedlacek CJ
Kits KD 1908 Horn M
Kits KD 1908 Daims H
Kits KD 1908 Berry D
Kits KD 1908 Loy A
Kits KD 1908 Wagner M
Kits KD 1908 Eichorst SA
Kits KD 1908 Mussmann M
Kits KD 1908 Wasmund K
Kits KD 1908 Herbold CW
Kits KD 1908 Sedlacek CJ
Sedlacek CJ 1909 Horn M
Sedlacek CJ 1909 Daims H
Sedlacek CJ 1909 Berry D
Sedlacek CJ 1909 Loy A
Sedlacek CJ 1909 Wagner M
Sedlacek CJ 1909 Eichorst SA
Sedlacek CJ 1909 Mussmann M
Sedlacek CJ 1909 Wasmund K
Sedlacek CJ 1909 Herbold CW
Sedlacek CJ 1909 Sedlacek CJ
Lebedeva EV 759 Horn M
Lebedeva EV 759 Daims H
Lebedeva EV 759 Berry D
Lebedeva EV 759 Loy A
Lebedeva EV 759 Wagner M
Lebedeva EV 759 Eichorst SA
Lebedeva EV 759 Mussmann M
Lebedeva EV 759 Wasmund K
Lebedeva EV 759 Herbold CW
Lebedeva EV 759 Sedlacek CJ
Han P 375 Horn M
Han P 375 Daims H
Han P 375 Berry D
Han P 375 Loy A
Han P 375 Wagner M
Han P 375 Eichorst SA
Han P 375 Mussmann M
Han P 375 Wasmund K
Han P 375 Herbold CW
Han P 375 Sedlacek CJ
Bulaev A 763 Horn M
Bulaev A 763 Daims H
Bulaev A 763 Berry D
Bulaev A 763 Loy A
Bulaev A 763 Wagner M
Bulaev A 763 Eichorst SA
Bulaev A 763 Mussmann M
Bulaev A 763 Wasmund K
Bulaev A 763 Herbold CW
Bulaev A 763 Sedlacek CJ
Pjevac P 1297 Horn M
Pjevac P 1297 Daims H
Pjevac P 1297 Berry D
Pjevac P 1297 Loy A
Pjevac P 1297 Wagner M
Pjevac P 1297 Eichorst SA
Pjevac P 1297 Mussmann M
Pjevac P 1297 Wasmund K
Pjevac P 1297 Herbold CW
Pjevac P 1297 Sedlacek CJ
Daebeler A 1900 Horn M
Daebeler A 1900 Daims H
Daebeler A 1900 Berry D
Daebeler A 1900 Loy A
Daebeler A 1900 Wagner M
Daebeler A 1900 Eichorst SA
Daebeler A 1900 Mussmann M
Daebeler A 1900 Wasmund K
Daebeler A 1900 Herbold CW
Daebeler A 1900 Sedlacek CJ
Romano S 1910 Horn M
Romano S 1910 Daims H
Romano S 1910 Berry D
Romano S 1910 Loy A
Romano S 1910 Wagner M
Romano S 1910 Eichorst SA
Romano S 1910 Mussmann M
Romano S 1910 Wasmund K
Romano S 1910 Herbold CW
Romano S 1910 Sedlacek CJ
Albertsen M 631 Horn M
Albertsen M 631 Daims H
Albertsen M 631 Berry D
Albertsen M 631 Loy A
Albertsen M 631 Wagner M
Albertsen M 631 Eichorst SA
Albertsen M 631 Mussmann M
Albertsen M 631 Wasmund K
Albertsen M 631 Herbold CW
Albertsen M 631 Sedlacek CJ
Stein LY 1911 Horn M
Stein LY 1911 Daims H
Stein LY 1911 Berry D
Stein LY 1911 Loy A
Stein LY 1911 Wagner M
Stein LY 1911 Eichorst SA
Stein LY 1911 Mussmann M
Stein LY 1911 Wasmund K
Stein LY 1911 Herbold CW
Stein LY 1911 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Pjevac P 1297 Horn M
Pjevac P 1297 Daims H
Pjevac P 1297 Berry D
Pjevac P 1297 Loy A
Pjevac P 1297 Wagner M
Pjevac P 1297 Eichorst SA
Pjevac P 1297 Mussmann M
Pjevac P 1297 Wasmund K
Pjevac P 1297 Herbold CW
Pjevac P 1297 Sedlacek CJ
Schauberger C 1897 Horn M
Schauberger C 1897 Daims H
Schauberger C 1897 Berry D
Schauberger C 1897 Loy A
Schauberger C 1897 Wagner M
Schauberger C 1897 Eichorst SA
Schauberger C 1897 Mussmann M
Schauberger C 1897 Wasmund K
Schauberger C 1897 Herbold CW
Schauberger C 1897 Sedlacek CJ
Poghosyan L 1898 Horn M
Poghosyan L 1898 Daims H
Poghosyan L 1898 Berry D
Poghosyan L 1898 Loy A
Poghosyan L 1898 Wagner M
Poghosyan L 1898 Eichorst SA
Poghosyan L 1898 Mussmann M
Poghosyan L 1898 Wasmund K
Poghosyan L 1898 Herbold CW
Poghosyan L 1898 Sedlacek CJ
Herbold CW 321 Horn M
Herbold CW 321 Daims H
Herbold CW 321 Berry D
Herbold CW 321 Loy A
Herbold CW 321 Wagner M
Herbold CW 321 Eichorst SA
Herbold CW 321 Mussmann M
Herbold CW 321 Wasmund K
Herbold CW 321 Herbold CW
Herbold CW 321 Sedlacek CJ
van Kessel MAHJ 1899 Horn M
van Kessel MAHJ 1899 Daims H
van Kessel MAHJ 1899 Berry D
van Kessel MAHJ 1899 Loy A
van Kessel MAHJ 1899 Wagner M
van Kessel MAHJ 1899 Eichorst SA
van Kessel MAHJ 1899 Mussmann M
van Kessel MAHJ 1899 Wasmund K
van Kessel MAHJ 1899 Herbold CW
van Kessel MAHJ 1899 Sedlacek CJ
Daebeler A 1900 Horn M
Daebeler A 1900 Daims H
Daebeler A 1900 Berry D
Daebeler A 1900 Loy A
Daebeler A 1900 Wagner M
Daebeler A 1900 Eichorst SA
Daebeler A 1900 Mussmann M
Daebeler A 1900 Wasmund K
Daebeler A 1900 Herbold CW
Daebeler A 1900 Sedlacek CJ
Steinberger M 1837 Horn M
Steinberger M 1837 Daims H
Steinberger M 1837 Berry D
Steinberger M 1837 Loy A
Steinberger M 1837 Wagner M
Steinberger M 1837 Eichorst SA
Steinberger M 1837 Mussmann M
Steinberger M 1837 Wasmund K
Steinberger M 1837 Herbold CW
Steinberger M 1837 Sedlacek CJ
Jetten MSM 487 Horn M
Jetten MSM 487 Daims H
Jetten MSM 487 Berry D
Jetten MSM 487 Loy A
Jetten MSM 487 Wagner M
Jetten MSM 487 Eichorst SA
Jetten MSM 487 Mussmann M
Jetten MSM 487 Wasmund K
Jetten MSM 487 Herbold CW
Jetten MSM 487 Sedlacek CJ
Luecker S 1901 Horn M
Luecker S 1901 Daims H
Luecker S 1901 Berry D
Luecker S 1901 Loy A
Luecker S 1901 Wagner M
Luecker S 1901 Eichorst SA
Luecker S 1901 Mussmann M
Luecker S 1901 Wasmund K
Luecker S 1901 Herbold CW
Luecker S 1901 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Böck D 1905 Horn M
Böck D 1905 Daims H
Böck D 1905 Berry D
Böck D 1905 Loy A
Böck D 1905 Wagner M
Böck D 1905 Eichorst SA
Böck D 1905 Mussmann M
Böck D 1905 Wasmund K
Böck D 1905 Herbold CW
Böck D 1905 Sedlacek CJ
Medeiros JM 1906 Horn M
Medeiros JM 1906 Daims H
Medeiros JM 1906 Berry D
Medeiros JM 1906 Loy A
Medeiros JM 1906 Wagner M
Medeiros JM 1906 Eichorst SA
Medeiros JM 1906 Mussmann M
Medeiros JM 1906 Wasmund K
Medeiros JM 1906 Herbold CW
Medeiros JM 1906 Sedlacek CJ
Tsao HF 1704 Horn M
Tsao HF 1704 Daims H
Tsao HF 1704 Berry D
Tsao HF 1704 Loy A
Tsao HF 1704 Wagner M
Tsao HF 1704 Eichorst SA
Tsao HF 1704 Mussmann M
Tsao HF 1704 Wasmund K
Tsao HF 1704 Herbold CW
Tsao HF 1704 Sedlacek CJ
Penz T 779 Horn M
Penz T 779 Daims H
Penz T 779 Berry D
Penz T 779 Loy A
Penz T 779 Wagner M
Penz T 779 Eichorst SA
Penz T 779 Mussmann M
Penz T 779 Wasmund K
Penz T 779 Herbold CW
Penz T 779 Sedlacek CJ
Weiss GL 1907 Horn M
Weiss GL 1907 Daims H
Weiss GL 1907 Berry D
Weiss GL 1907 Loy A
Weiss GL 1907 Wagner M
Weiss GL 1907 Eichorst SA
Weiss GL 1907 Mussmann M
Weiss GL 1907 Wasmund K
Weiss GL 1907 Herbold CW
Weiss GL 1907 Sedlacek CJ
Aistleitner K 258 Horn M
Aistleitner K 258 Daims H
Aistleitner K 258 Berry D
Aistleitner K 258 Loy A
Aistleitner K 258 Wagner M
Aistleitner K 258 Eichorst SA
Aistleitner K 258 Mussmann M
Aistleitner K 258 Wasmund K
Aistleitner K 258 Herbold CW
Aistleitner K 258 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Pilhofer M 540 Horn M
Pilhofer M 540 Daims H
Pilhofer M 540 Berry D
Pilhofer M 540 Loy A
Pilhofer M 540 Wagner M
Pilhofer M 540 Eichorst SA
Pilhofer M 540 Mussmann M
Pilhofer M 540 Wasmund K
Pilhofer M 540 Herbold CW
Pilhofer M 540 Sedlacek CJ
Ladurner A 1960 Horn M
Ladurner A 1960 Daims H
Ladurner A 1960 Berry D
Ladurner A 1960 Loy A
Ladurner A 1960 Wagner M
Ladurner A 1960 Eichorst SA
Ladurner A 1960 Mussmann M
Ladurner A 1960 Wasmund K
Ladurner A 1960 Herbold CW
Ladurner A 1960 Sedlacek CJ
Zehl M 1316 Horn M
Zehl M 1316 Daims H
Zehl M 1316 Berry D
Zehl M 1316 Loy A
Zehl M 1316 Wagner M
Zehl M 1316 Eichorst SA
Zehl M 1316 Mussmann M
Zehl M 1316 Wasmund K
Zehl M 1316 Herbold CW
Zehl M 1316 Sedlacek CJ
Grienke U 1961 Horn M
Grienke U 1961 Daims H
Grienke U 1961 Berry D
Grienke U 1961 Loy A
Grienke U 1961 Wagner M
Grienke U 1961 Eichorst SA
Grienke U 1961 Mussmann M
Grienke U 1961 Wasmund K
Grienke U 1961 Herbold CW
Grienke U 1961 Sedlacek CJ
Hofstadler C 1962 Horn M
Hofstadler C 1962 Daims H
Hofstadler C 1962 Berry D
Hofstadler C 1962 Loy A
Hofstadler C 1962 Wagner M
Hofstadler C 1962 Eichorst SA
Hofstadler C 1962 Mussmann M
Hofstadler C 1962 Wasmund K
Hofstadler C 1962 Herbold CW
Hofstadler C 1962 Sedlacek CJ
Faur N 1963 Horn M
Faur N 1963 Daims H
Faur N 1963 Berry D
Faur N 1963 Loy A
Faur N 1963 Wagner M
Faur N 1963 Eichorst SA
Faur N 1963 Mussmann M
Faur N 1963 Wasmund K
Faur N 1963 Herbold CW
Faur N 1963 Sedlacek CJ
Pereira FC 1844 Horn M
Pereira FC 1844 Daims H
Pereira FC 1844 Berry D
Pereira FC 1844 Loy A
Pereira FC 1844 Wagner M
Pereira FC 1844 Eichorst SA
Pereira FC 1844 Mussmann M
Pereira FC 1844 Wasmund K
Pereira FC 1844 Herbold CW
Pereira FC 1844 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Dirsch VM 1964 Horn M
Dirsch VM 1964 Daims H
Dirsch VM 1964 Berry D
Dirsch VM 1964 Loy A
Dirsch VM 1964 Wagner M
Dirsch VM 1964 Eichorst SA
Dirsch VM 1964 Mussmann M
Dirsch VM 1964 Wasmund K
Dirsch VM 1964 Herbold CW
Dirsch VM 1964 Sedlacek CJ
Rollinger JM 1965 Horn M
Rollinger JM 1965 Daims H
Rollinger JM 1965 Berry D
Rollinger JM 1965 Loy A
Rollinger JM 1965 Wagner M
Rollinger JM 1965 Eichorst SA
Rollinger JM 1965 Mussmann M
Rollinger JM 1965 Wasmund K
Rollinger JM 1965 Herbold CW
Rollinger JM 1965 Sedlacek CJ
Collingro A 293 Horn M
Collingro A 293 Daims H
Collingro A 293 Berry D
Collingro A 293 Loy A
Collingro A 293 Wagner M
Collingro A 293 Eichorst SA
Collingro A 293 Mussmann M
Collingro A 293 Wasmund K
Collingro A 293 Herbold CW
Collingro A 293 Sedlacek CJ
Köstlbacher S 1893 Horn M
Köstlbacher S 1893 Daims H
Köstlbacher S 1893 Berry D
Köstlbacher S 1893 Loy A
Köstlbacher S 1893 Wagner M
Köstlbacher S 1893 Eichorst SA
Köstlbacher S 1893 Mussmann M
Köstlbacher S 1893 Wasmund K
Köstlbacher S 1893 Herbold CW
Köstlbacher S 1893 Sedlacek CJ
Mussmann M 896 Horn M
Mussmann M 896 Daims H
Mussmann M 896 Berry D
Mussmann M 896 Loy A
Mussmann M 896 Wagner M
Mussmann M 896 Eichorst SA
Mussmann M 896 Mussmann M
Mussmann M 896 Wasmund K
Mussmann M 896 Herbold CW
Mussmann M 896 Sedlacek CJ
Stepanauskas R 342 Horn M
Stepanauskas R 342 Daims H
Stepanauskas R 342 Berry D
Stepanauskas R 342 Loy A
Stepanauskas R 342 Wagner M
Stepanauskas R 342 Eichorst SA
Stepanauskas R 342 Mussmann M
Stepanauskas R 342 Wasmund K
Stepanauskas R 342 Herbold CW
Stepanauskas R 342 Sedlacek CJ
Hallam SJ 1894 Horn M
Hallam SJ 1894 Daims H
Hallam SJ 1894 Berry D
Hallam SJ 1894 Loy A
Hallam SJ 1894 Wagner M
Hallam SJ 1894 Eichorst SA
Hallam SJ 1894 Mussmann M
Hallam SJ 1894 Wasmund K
Hallam SJ 1894 Herbold CW
Hallam SJ 1894 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Tsao HF 1704 Horn M
Tsao HF 1704 Daims H
Tsao HF 1704 Berry D
Tsao HF 1704 Loy A
Tsao HF 1704 Wagner M
Tsao HF 1704 Eichorst SA
Tsao HF 1704 Mussmann M
Tsao HF 1704 Wasmund K
Tsao HF 1704 Herbold CW
Tsao HF 1704 Sedlacek CJ
Scheikl U 1703 Horn M
Scheikl U 1703 Daims H
Scheikl U 1703 Berry D
Scheikl U 1703 Loy A
Scheikl U 1703 Wagner M
Scheikl U 1703 Eichorst SA
Scheikl U 1703 Mussmann M
Scheikl U 1703 Wasmund K
Scheikl U 1703 Herbold CW
Scheikl U 1703 Sedlacek CJ
Volland JM 661 Horn M
Volland JM 661 Daims H
Volland JM 661 Berry D
Volland JM 661 Loy A
Volland JM 661 Wagner M
Volland JM 661 Eichorst SA
Volland JM 661 Mussmann M
Volland JM 661 Wasmund K
Volland JM 661 Herbold CW
Volland JM 661 Sedlacek CJ
Köhsler M 1892 Horn M
Köhsler M 1892 Daims H
Köhsler M 1892 Berry D
Köhsler M 1892 Loy A
Köhsler M 1892 Wagner M
Köhsler M 1892 Eichorst SA
Köhsler M 1892 Mussmann M
Köhsler M 1892 Wasmund K
Köhsler M 1892 Herbold CW
Köhsler M 1892 Sedlacek CJ
Bright M 262 Horn M
Bright M 262 Daims H
Bright M 262 Berry D
Bright M 262 Loy A
Bright M 262 Wagner M
Bright M 262 Eichorst SA
Bright M 262 Mussmann M
Bright M 262 Wasmund K
Bright M 262 Herbold CW
Bright M 262 Sedlacek CJ
Walochnik J 427 Horn M
Walochnik J 427 Daims H
Walochnik J 427 Berry D
Walochnik J 427 Loy A
Walochnik J 427 Wagner M
Walochnik J 427 Eichorst SA
Walochnik J 427 Mussmann M
Walochnik J 427 Wasmund K
Walochnik J 427 Herbold CW
Walochnik J 427 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
König L 542 Horn M
König L 542 Daims H
König L 542 Berry D
König L 542 Loy A
König L 542 Wagner M
König L 542 Eichorst SA
König L 542 Mussmann M
König L 542 Wasmund K
König L 542 Herbold CW
König L 542 Sedlacek CJ
Siegl A 871 Horn M
Siegl A 871 Daims H
Siegl A 871 Berry D
Siegl A 871 Loy A
Siegl A 871 Wagner M
Siegl A 871 Eichorst SA
Siegl A 871 Mussmann M
Siegl A 871 Wasmund K
Siegl A 871 Herbold CW
Siegl A 871 Sedlacek CJ
Penz T 779 Horn M
Penz T 779 Daims H
Penz T 779 Berry D
Penz T 779 Loy A
Penz T 779 Wagner M
Penz T 779 Eichorst SA
Penz T 779 Mussmann M
Penz T 779 Wasmund K
Penz T 779 Herbold CW
Penz T 779 Sedlacek CJ
Haider S 958 Horn M
Haider S 958 Daims H
Haider S 958 Berry D
Haider S 958 Loy A
Haider S 958 Wagner M
Haider S 958 Eichorst SA
Haider S 958 Mussmann M
Haider S 958 Wasmund K
Haider S 958 Herbold CW
Haider S 958 Sedlacek CJ
Wentrup C 1125 Horn M
Wentrup C 1125 Daims H
Wentrup C 1125 Berry D
Wentrup C 1125 Loy A
Wentrup C 1125 Wagner M
Wentrup C 1125 Eichorst SA
Wentrup C 1125 Mussmann M
Wentrup C 1125 Wasmund K
Wentrup C 1125 Herbold CW
Wentrup C 1125 Sedlacek CJ
Polzin J 1889 Horn M
Polzin J 1889 Daims H
Polzin J 1889 Berry D
Polzin J 1889 Loy A
Polzin J 1889 Wagner M
Polzin J 1889 Eichorst SA
Polzin J 1889 Mussmann M
Polzin J 1889 Wasmund K
Polzin J 1889 Herbold CW
Polzin J 1889 Sedlacek CJ
Mann E 1890 Horn M
Mann E 1890 Daims H
Mann E 1890 Berry D
Mann E 1890 Loy A
Mann E 1890 Wagner M
Mann E 1890 Eichorst SA
Mann E 1890 Mussmann M
Mann E 1890 Wasmund K
Mann E 1890 Herbold CW
Mann E 1890 Sedlacek CJ
Schmitz-Esser S 593 Horn M
Schmitz-Esser S 593 Daims H
Schmitz-Esser S 593 Berry D
Schmitz-Esser S 593 Loy A
Schmitz-Esser S 593 Wagner M
Schmitz-Esser S 593 Eichorst SA
Schmitz-Esser S 593 Mussmann M
Schmitz-Esser S 593 Wasmund K
Schmitz-Esser S 593 Herbold CW
Schmitz-Esser S 593 Sedlacek CJ
Domman D 299 Horn M
Domman D 299 Daims H
Domman D 299 Berry D
Domman D 299 Loy A
Domman D 299 Wagner M
Domman D 299 Eichorst SA
Domman D 299 Mussmann M
Domman D 299 Wasmund K
Domman D 299 Herbold CW
Domman D 299 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Oswald K 1863 Horn M
Oswald K 1863 Daims H
Oswald K 1863 Berry D
Oswald K 1863 Loy A
Oswald K 1863 Wagner M
Oswald K 1863 Eichorst SA
Oswald K 1863 Mussmann M
Oswald K 1863 Wasmund K
Oswald K 1863 Herbold CW
Oswald K 1863 Sedlacek CJ
Graf JS 1864 Horn M
Graf JS 1864 Daims H
Graf JS 1864 Berry D
Graf JS 1864 Loy A
Graf JS 1864 Wagner M
Graf JS 1864 Eichorst SA
Graf JS 1864 Mussmann M
Graf JS 1864 Wasmund K
Graf JS 1864 Herbold CW
Graf JS 1864 Sedlacek CJ
Littmann S 1902 Horn M
Littmann S 1902 Daims H
Littmann S 1902 Berry D
Littmann S 1902 Loy A
Littmann S 1902 Wagner M
Littmann S 1902 Eichorst SA
Littmann S 1902 Mussmann M
Littmann S 1902 Wasmund K
Littmann S 1902 Herbold CW
Littmann S 1902 Sedlacek CJ
Tienken D 1903 Horn M
Tienken D 1903 Daims H
Tienken D 1903 Berry D
Tienken D 1903 Loy A
Tienken D 1903 Wagner M
Tienken D 1903 Eichorst SA
Tienken D 1903 Mussmann M
Tienken D 1903 Wasmund K
Tienken D 1903 Herbold CW
Tienken D 1903 Sedlacek CJ
Brand A 1867 Horn M
Brand A 1867 Daims H
Brand A 1867 Berry D
Brand A 1867 Loy A
Brand A 1867 Wagner M
Brand A 1867 Eichorst SA
Brand A 1867 Mussmann M
Brand A 1867 Wasmund K
Brand A 1867 Herbold CW
Brand A 1867 Sedlacek CJ
Wehrli B 1868 Horn M
Wehrli B 1868 Daims H
Wehrli B 1868 Berry D
Wehrli B 1868 Loy A
Wehrli B 1868 Wagner M
Wehrli B 1868 Eichorst SA
Wehrli B 1868 Mussmann M
Wehrli B 1868 Wasmund K
Wehrli B 1868 Herbold CW
Wehrli B 1868 Sedlacek CJ
Albertsen M 631 Horn M
Albertsen M 631 Daims H
Albertsen M 631 Berry D
Albertsen M 631 Loy A
Albertsen M 631 Wagner M
Albertsen M 631 Eichorst SA
Albertsen M 631 Mussmann M
Albertsen M 631 Wasmund K
Albertsen M 631 Herbold CW
Albertsen M 631 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Kuypers MM 1238 Horn M
Kuypers MM 1238 Daims H
Kuypers MM 1238 Berry D
Kuypers MM 1238 Loy A
Kuypers MM 1238 Wagner M
Kuypers MM 1238 Eichorst SA
Kuypers MM 1238 Mussmann M
Kuypers MM 1238 Wasmund K
Kuypers MM 1238 Herbold CW
Kuypers MM 1238 Sedlacek CJ
Schubert CJ 1755 Horn M
Schubert CJ 1755 Daims H
Schubert CJ 1755 Berry D
Schubert CJ 1755 Loy A
Schubert CJ 1755 Wagner M
Schubert CJ 1755 Eichorst SA
Schubert CJ 1755 Mussmann M
Schubert CJ 1755 Wasmund K
Schubert CJ 1755 Herbold CW
Schubert CJ 1755 Sedlacek CJ
Milucka J 1019 Horn M
Milucka J 1019 Daims H
Milucka J 1019 Berry D
Milucka J 1019 Loy A
Milucka J 1019 Wagner M
Milucka J 1019 Eichorst SA
Milucka J 1019 Mussmann M
Milucka J 1019 Wasmund K
Milucka J 1019 Herbold CW
Milucka J 1019 Sedlacek CJ
Singer E 1858 Horn M
Singer E 1858 Daims H
Singer E 1858 Berry D
Singer E 1858 Loy A
Singer E 1858 Wagner M
Singer E 1858 Eichorst SA
Singer E 1858 Mussmann M
Singer E 1858 Wasmund K
Singer E 1858 Herbold CW
Singer E 1858 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Woyke T 616 Horn M
Woyke T 616 Daims H
Woyke T 616 Berry D
Woyke T 616 Loy A
Woyke T 616 Wagner M
Woyke T 616 Eichorst SA
Woyke T 616 Mussmann M
Woyke T 616 Wasmund K
Woyke T 616 Herbold CW
Woyke T 616 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Gutierrez T 642 Horn M
Gutierrez T 642 Daims H
Gutierrez T 642 Berry D
Gutierrez T 642 Loy A
Gutierrez T 642 Wagner M
Gutierrez T 642 Eichorst SA
Gutierrez T 642 Mussmann M
Gutierrez T 642 Wasmund K
Gutierrez T 642 Herbold CW
Gutierrez T 642 Sedlacek CJ
Harrison JP 1767 Horn M
Harrison JP 1767 Daims H
Harrison JP 1767 Berry D
Harrison JP 1767 Loy A
Harrison JP 1767 Wagner M
Harrison JP 1767 Eichorst SA
Harrison JP 1767 Mussmann M
Harrison JP 1767 Wasmund K
Harrison JP 1767 Herbold CW
Harrison JP 1767 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Lang M 1874 Horn M
Lang M 1874 Daims H
Lang M 1874 Berry D
Lang M 1874 Loy A
Lang M 1874 Wagner M
Lang M 1874 Eichorst SA
Lang M 1874 Mussmann M
Lang M 1874 Wasmund K
Lang M 1874 Herbold CW
Lang M 1874 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Passecker K 1875 Horn M
Passecker K 1875 Daims H
Passecker K 1875 Berry D
Passecker K 1875 Loy A
Passecker K 1875 Wagner M
Passecker K 1875 Eichorst SA
Passecker K 1875 Mussmann M
Passecker K 1875 Wasmund K
Passecker K 1875 Herbold CW
Passecker K 1875 Sedlacek CJ
Mesteri I 1876 Horn M
Mesteri I 1876 Daims H
Mesteri I 1876 Berry D
Mesteri I 1876 Loy A
Mesteri I 1876 Wagner M
Mesteri I 1876 Eichorst SA
Mesteri I 1876 Mussmann M
Mesteri I 1876 Wasmund K
Mesteri I 1876 Herbold CW
Mesteri I 1876 Sedlacek CJ
Bhuju S 1877 Horn M
Bhuju S 1877 Daims H
Bhuju S 1877 Berry D
Bhuju S 1877 Loy A
Bhuju S 1877 Wagner M
Bhuju S 1877 Eichorst SA
Bhuju S 1877 Mussmann M
Bhuju S 1877 Wasmund K
Bhuju S 1877 Herbold CW
Bhuju S 1877 Sedlacek CJ
Ebner F 1878 Horn M
Ebner F 1878 Daims H
Ebner F 1878 Berry D
Ebner F 1878 Loy A
Ebner F 1878 Wagner M
Ebner F 1878 Eichorst SA
Ebner F 1878 Mussmann M
Ebner F 1878 Wasmund K
Ebner F 1878 Herbold CW
Ebner F 1878 Sedlacek CJ
Sedlyarov V 1879 Horn M
Sedlyarov V 1879 Daims H
Sedlyarov V 1879 Berry D
Sedlyarov V 1879 Loy A
Sedlyarov V 1879 Wagner M
Sedlyarov V 1879 Eichorst SA
Sedlyarov V 1879 Mussmann M
Sedlyarov V 1879 Wasmund K
Sedlyarov V 1879 Herbold CW
Sedlyarov V 1879 Sedlacek CJ
Evstatiev R 1880 Horn M
Evstatiev R 1880 Daims H
Evstatiev R 1880 Berry D
Evstatiev R 1880 Loy A
Evstatiev R 1880 Wagner M
Evstatiev R 1880 Eichorst SA
Evstatiev R 1880 Mussmann M
Evstatiev R 1880 Wasmund K
Evstatiev R 1880 Herbold CW
Evstatiev R 1880 Sedlacek CJ
Dammann K 1881 Horn M
Dammann K 1881 Daims H
Dammann K 1881 Berry D
Dammann K 1881 Loy A
Dammann K 1881 Wagner M
Dammann K 1881 Eichorst SA
Dammann K 1881 Mussmann M
Dammann K 1881 Wasmund K
Dammann K 1881 Herbold CW
Dammann K 1881 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Kuzyk O 323 Horn M
Kuzyk O 323 Daims H
Kuzyk O 323 Berry D
Kuzyk O 323 Loy A
Kuzyk O 323 Wagner M
Kuzyk O 323 Eichorst SA
Kuzyk O 323 Mussmann M
Kuzyk O 323 Wasmund K
Kuzyk O 323 Herbold CW
Kuzyk O 323 Sedlacek CJ
Kovarik P 1882 Horn M
Kovarik P 1882 Daims H
Kovarik P 1882 Berry D
Kovarik P 1882 Loy A
Kovarik P 1882 Wagner M
Kovarik P 1882 Eichorst SA
Kovarik P 1882 Mussmann M
Kovarik P 1882 Wasmund K
Kovarik P 1882 Herbold CW
Kovarik P 1882 Sedlacek CJ
Khare V 1883 Horn M
Khare V 1883 Daims H
Khare V 1883 Berry D
Khare V 1883 Loy A
Khare V 1883 Wagner M
Khare V 1883 Eichorst SA
Khare V 1883 Mussmann M
Khare V 1883 Wasmund K
Khare V 1883 Herbold CW
Khare V 1883 Sedlacek CJ
Beibel M 1884 Horn M
Beibel M 1884 Daims H
Beibel M 1884 Berry D
Beibel M 1884 Loy A
Beibel M 1884 Wagner M
Beibel M 1884 Eichorst SA
Beibel M 1884 Mussmann M
Beibel M 1884 Wasmund K
Beibel M 1884 Herbold CW
Beibel M 1884 Sedlacek CJ
Roma G 1885 Horn M
Roma G 1885 Daims H
Roma G 1885 Berry D
Roma G 1885 Loy A
Roma G 1885 Wagner M
Roma G 1885 Eichorst SA
Roma G 1885 Mussmann M
Roma G 1885 Wasmund K
Roma G 1885 Herbold CW
Roma G 1885 Sedlacek CJ
Meisner-Kober N 1886 Horn M
Meisner-Kober N 1886 Daims H
Meisner-Kober N 1886 Berry D
Meisner-Kober N 1886 Loy A
Meisner-Kober N 1886 Wagner M
Meisner-Kober N 1886 Eichorst SA
Meisner-Kober N 1886 Mussmann M
Meisner-Kober N 1886 Wasmund K
Meisner-Kober N 1886 Herbold CW
Meisner-Kober N 1886 Sedlacek CJ
Gasche C 1888 Horn M
Gasche C 1888 Daims H
Gasche C 1888 Berry D
Gasche C 1888 Loy A
Gasche C 1888 Wagner M
Gasche C 1888 Eichorst SA
Gasche C 1888 Mussmann M
Gasche C 1888 Wasmund K
Gasche C 1888 Herbold CW
Gasche C 1888 Sedlacek CJ
Wasmund K 1447 Horn M
Wasmund K 1447 Daims H
Wasmund K 1447 Berry D
Wasmund K 1447 Loy A
Wasmund K 1447 Wagner M
Wasmund K 1447 Eichorst SA
Wasmund K 1447 Mussmann M
Wasmund K 1447 Wasmund K
Wasmund K 1447 Herbold CW
Wasmund K 1447 Sedlacek CJ
Mußmann M 1747 Horn M
Mußmann M 1747 Daims H
Mußmann M 1747 Berry D
Mußmann M 1747 Loy A
Mußmann M 1747 Wagner M
Mußmann M 1747 Eichorst SA
Mußmann M 1747 Mussmann M
Mußmann M 1747 Wasmund K
Mußmann M 1747 Herbold CW
Mußmann M 1747 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Schulz F 296 Horn M
Schulz F 296 Daims H
Schulz F 296 Berry D
Schulz F 296 Loy A
Schulz F 296 Wagner M
Schulz F 296 Eichorst SA
Schulz F 296 Mussmann M
Schulz F 296 Wasmund K
Schulz F 296 Herbold CW
Schulz F 296 Sedlacek CJ
Yutin N 1873 Horn M
Yutin N 1873 Daims H
Yutin N 1873 Berry D
Yutin N 1873 Loy A
Yutin N 1873 Wagner M
Yutin N 1873 Eichorst SA
Yutin N 1873 Mussmann M
Yutin N 1873 Wasmund K
Yutin N 1873 Herbold CW
Yutin N 1873 Sedlacek CJ
Ivanova NN 1870 Horn M
Ivanova NN 1870 Daims H
Ivanova NN 1870 Berry D
Ivanova NN 1870 Loy A
Ivanova NN 1870 Wagner M
Ivanova NN 1870 Eichorst SA
Ivanova NN 1870 Mussmann M
Ivanova NN 1870 Wasmund K
Ivanova NN 1870 Herbold CW
Ivanova NN 1870 Sedlacek CJ
Ortega DR 1871 Horn M
Ortega DR 1871 Daims H
Ortega DR 1871 Berry D
Ortega DR 1871 Loy A
Ortega DR 1871 Wagner M
Ortega DR 1871 Eichorst SA
Ortega DR 1871 Mussmann M
Ortega DR 1871 Wasmund K
Ortega DR 1871 Herbold CW
Ortega DR 1871 Sedlacek CJ
Lee TK 277 Horn M
Lee TK 277 Daims H
Lee TK 277 Berry D
Lee TK 277 Loy A
Lee TK 277 Wagner M
Lee TK 277 Eichorst SA
Lee TK 277 Mussmann M
Lee TK 277 Wasmund K
Lee TK 277 Herbold CW
Lee TK 277 Sedlacek CJ
Vierheilig J 1298 Horn M
Vierheilig J 1298 Daims H
Vierheilig J 1298 Berry D
Vierheilig J 1298 Loy A
Vierheilig J 1298 Wagner M
Vierheilig J 1298 Eichorst SA
Vierheilig J 1298 Mussmann M
Vierheilig J 1298 Wasmund K
Vierheilig J 1298 Herbold CW
Vierheilig J 1298 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Jensen GJ 543 Horn M
Jensen GJ 543 Daims H
Jensen GJ 543 Berry D
Jensen GJ 543 Loy A
Jensen GJ 543 Wagner M
Jensen GJ 543 Eichorst SA
Jensen GJ 543 Mussmann M
Jensen GJ 543 Wasmund K
Jensen GJ 543 Herbold CW
Jensen GJ 543 Sedlacek CJ
Kyrpides NC 613 Horn M
Kyrpides NC 613 Daims H
Kyrpides NC 613 Berry D
Kyrpides NC 613 Loy A
Kyrpides NC 613 Wagner M
Kyrpides NC 613 Eichorst SA
Kyrpides NC 613 Mussmann M
Kyrpides NC 613 Wasmund K
Kyrpides NC 613 Herbold CW
Kyrpides NC 613 Sedlacek CJ
Koonin EV 1872 Horn M
Koonin EV 1872 Daims H
Koonin EV 1872 Berry D
Koonin EV 1872 Loy A
Koonin EV 1872 Wagner M
Koonin EV 1872 Eichorst SA
Koonin EV 1872 Mussmann M
Koonin EV 1872 Wasmund K
Koonin EV 1872 Herbold CW
Koonin EV 1872 Sedlacek CJ
Woyke T 616 Horn M
Woyke T 616 Daims H
Woyke T 616 Berry D
Woyke T 616 Loy A
Woyke T 616 Wagner M
Woyke T 616 Eichorst SA
Woyke T 616 Mussmann M
Woyke T 616 Wasmund K
Woyke T 616 Herbold CW
Woyke T 616 Sedlacek CJ
Schueller K 1891 Horn M
Schueller K 1891 Daims H
Schueller K 1891 Berry D
Schueller K 1891 Loy A
Schueller K 1891 Wagner M
Schueller K 1891 Eichorst SA
Schueller K 1891 Mussmann M
Schueller K 1891 Wasmund K
Schueller K 1891 Herbold CW
Schueller K 1891 Sedlacek CJ
Riva A 1551 Horn M
Riva A 1551 Daims H
Riva A 1551 Berry D
Riva A 1551 Loy A
Riva A 1551 Wagner M
Riva A 1551 Eichorst SA
Riva A 1551 Mussmann M
Riva A 1551 Wasmund K
Riva A 1551 Herbold CW
Riva A 1551 Sedlacek CJ
Pfeiffer S 965 Horn M
Pfeiffer S 965 Daims H
Pfeiffer S 965 Berry D
Pfeiffer S 965 Loy A
Pfeiffer S 965 Wagner M
Pfeiffer S 965 Eichorst SA
Pfeiffer S 965 Mussmann M
Pfeiffer S 965 Wasmund K
Pfeiffer S 965 Herbold CW
Pfeiffer S 965 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Somoza V 1857 Horn M
Somoza V 1857 Daims H
Somoza V 1857 Berry D
Somoza V 1857 Loy A
Somoza V 1857 Wagner M
Somoza V 1857 Eichorst SA
Somoza V 1857 Mussmann M
Somoza V 1857 Wasmund K
Somoza V 1857 Herbold CW
Somoza V 1857 Sedlacek CJ
Zojer M 1860 Horn M
Zojer M 1860 Daims H
Zojer M 1860 Berry D
Zojer M 1860 Loy A
Zojer M 1860 Wagner M
Zojer M 1860 Eichorst SA
Zojer M 1860 Mussmann M
Zojer M 1860 Wasmund K
Zojer M 1860 Herbold CW
Zojer M 1860 Sedlacek CJ
Schuster LN 1861 Horn M
Schuster LN 1861 Daims H
Schuster LN 1861 Berry D
Schuster LN 1861 Loy A
Schuster LN 1861 Wagner M
Schuster LN 1861 Eichorst SA
Schuster LN 1861 Mussmann M
Schuster LN 1861 Wasmund K
Schuster LN 1861 Herbold CW
Schuster LN 1861 Sedlacek CJ
Schulz F 296 Horn M
Schulz F 296 Daims H
Schulz F 296 Berry D
Schulz F 296 Loy A
Schulz F 296 Wagner M
Schulz F 296 Eichorst SA
Schulz F 296 Mussmann M
Schulz F 296 Wasmund K
Schulz F 296 Herbold CW
Schulz F 296 Sedlacek CJ
Pfundner A 1862 Horn M
Pfundner A 1862 Daims H
Pfundner A 1862 Berry D
Pfundner A 1862 Loy A
Pfundner A 1862 Wagner M
Pfundner A 1862 Eichorst SA
Pfundner A 1862 Mussmann M
Pfundner A 1862 Wasmund K
Pfundner A 1862 Herbold CW
Pfundner A 1862 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Rattei T 307 Horn M
Rattei T 307 Daims H
Rattei T 307 Berry D
Rattei T 307 Loy A
Rattei T 307 Wagner M
Rattei T 307 Eichorst SA
Rattei T 307 Mussmann M
Rattei T 307 Wasmund K
Rattei T 307 Herbold CW
Rattei T 307 Sedlacek CJ
Sauder LA 1723 Horn M
Sauder LA 1723 Daims H
Sauder LA 1723 Berry D
Sauder LA 1723 Loy A
Sauder LA 1723 Wagner M
Sauder LA 1723 Eichorst SA
Sauder LA 1723 Mussmann M
Sauder LA 1723 Wasmund K
Sauder LA 1723 Herbold CW
Sauder LA 1723 Sedlacek CJ
Albertsen M 631 Horn M
Albertsen M 631 Daims H
Albertsen M 631 Berry D
Albertsen M 631 Loy A
Albertsen M 631 Wagner M
Albertsen M 631 Eichorst SA
Albertsen M 631 Mussmann M
Albertsen M 631 Wasmund K
Albertsen M 631 Herbold CW
Albertsen M 631 Sedlacek CJ
Engel K 1724 Horn M
Engel K 1724 Daims H
Engel K 1724 Berry D
Engel K 1724 Loy A
Engel K 1724 Wagner M
Engel K 1724 Eichorst SA
Engel K 1724 Mussmann M
Engel K 1724 Wasmund K
Engel K 1724 Herbold CW
Engel K 1724 Sedlacek CJ
Schwarz J 337 Horn M
Schwarz J 337 Daims H
Schwarz J 337 Berry D
Schwarz J 337 Loy A
Schwarz J 337 Wagner M
Schwarz J 337 Eichorst SA
Schwarz J 337 Mussmann M
Schwarz J 337 Wasmund K
Schwarz J 337 Herbold CW
Schwarz J 337 Sedlacek CJ
Nielsen PH 448 Horn M
Nielsen PH 448 Daims H
Nielsen PH 448 Berry D
Nielsen PH 448 Loy A
Nielsen PH 448 Wagner M
Nielsen PH 448 Eichorst SA
Nielsen PH 448 Mussmann M
Nielsen PH 448 Wasmund K
Nielsen PH 448 Herbold CW
Nielsen PH 448 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Neufeld JD 853 Horn M
Neufeld JD 853 Daims H
Neufeld JD 853 Berry D
Neufeld JD 853 Loy A
Neufeld JD 853 Wagner M
Neufeld JD 853 Eichorst SA
Neufeld JD 853 Mussmann M
Neufeld JD 853 Wasmund K
Neufeld JD 853 Herbold CW
Neufeld JD 853 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Pfann C 1727 Horn M
Pfann C 1727 Daims H
Pfann C 1727 Berry D
Pfann C 1727 Loy A
Pfann C 1727 Wagner M
Pfann C 1727 Eichorst SA
Pfann C 1727 Mussmann M
Pfann C 1727 Wasmund K
Pfann C 1727 Herbold CW
Pfann C 1727 Sedlacek CJ
Steinberger M 1837 Horn M
Steinberger M 1837 Daims H
Steinberger M 1837 Berry D
Steinberger M 1837 Loy A
Steinberger M 1837 Wagner M
Steinberger M 1837 Eichorst SA
Steinberger M 1837 Mussmann M
Steinberger M 1837 Wasmund K
Steinberger M 1837 Herbold CW
Steinberger M 1837 Sedlacek CJ
Hanson B 1425 Horn M
Hanson B 1425 Daims H
Hanson B 1425 Berry D
Hanson B 1425 Loy A
Hanson B 1425 Wagner M
Hanson B 1425 Eichorst SA
Hanson B 1425 Mussmann M
Hanson B 1425 Wasmund K
Hanson B 1425 Herbold CW
Hanson B 1425 Sedlacek CJ
Herp S 1732 Horn M
Herp S 1732 Daims H
Herp S 1732 Berry D
Herp S 1732 Loy A
Herp S 1732 Wagner M
Herp S 1732 Eichorst SA
Herp S 1732 Mussmann M
Herp S 1732 Wasmund K
Herp S 1732 Herbold CW
Herp S 1732 Sedlacek CJ
Brugiroux S 803 Horn M
Brugiroux S 803 Daims H
Brugiroux S 803 Berry D
Brugiroux S 803 Loy A
Brugiroux S 803 Wagner M
Brugiroux S 803 Eichorst SA
Brugiroux S 803 Mussmann M
Brugiroux S 803 Wasmund K
Brugiroux S 803 Herbold CW
Brugiroux S 803 Sedlacek CJ
Gomes Neto JC 1838 Horn M
Gomes Neto JC 1838 Daims H
Gomes Neto JC 1838 Berry D
Gomes Neto JC 1838 Loy A
Gomes Neto JC 1838 Wagner M
Gomes Neto JC 1838 Eichorst SA
Gomes Neto JC 1838 Mussmann M
Gomes Neto JC 1838 Wasmund K
Gomes Neto JC 1838 Herbold CW
Gomes Neto JC 1838 Sedlacek CJ
Boekschoten MV 1839 Horn M
Boekschoten MV 1839 Daims H
Boekschoten MV 1839 Berry D
Boekschoten MV 1839 Loy A
Boekschoten MV 1839 Wagner M
Boekschoten MV 1839 Eichorst SA
Boekschoten MV 1839 Mussmann M
Boekschoten MV 1839 Wasmund K
Boekschoten MV 1839 Herbold CW
Boekschoten MV 1839 Sedlacek CJ
Schwab C 513 Horn M
Schwab C 513 Daims H
Schwab C 513 Berry D
Schwab C 513 Loy A
Schwab C 513 Wagner M
Schwab C 513 Eichorst SA
Schwab C 513 Mussmann M
Schwab C 513 Wasmund K
Schwab C 513 Herbold CW
Schwab C 513 Sedlacek CJ
Urich T 515 Horn M
Urich T 515 Daims H
Urich T 515 Berry D
Urich T 515 Loy A
Urich T 515 Wagner M
Urich T 515 Eichorst SA
Urich T 515 Mussmann M
Urich T 515 Wasmund K
Urich T 515 Herbold CW
Urich T 515 Sedlacek CJ
Ramer-Tait AE 1840 Horn M
Ramer-Tait AE 1840 Daims H
Ramer-Tait AE 1840 Berry D
Ramer-Tait AE 1840 Loy A
Ramer-Tait AE 1840 Wagner M
Ramer-Tait AE 1840 Eichorst SA
Ramer-Tait AE 1840 Mussmann M
Ramer-Tait AE 1840 Wasmund K
Ramer-Tait AE 1840 Herbold CW
Ramer-Tait AE 1840 Sedlacek CJ
Rattei T 307 Horn M
Rattei T 307 Daims H
Rattei T 307 Berry D
Rattei T 307 Loy A
Rattei T 307 Wagner M
Rattei T 307 Eichorst SA
Rattei T 307 Mussmann M
Rattei T 307 Wasmund K
Rattei T 307 Herbold CW
Rattei T 307 Sedlacek CJ
Stecher B 801 Horn M
Stecher B 801 Daims H
Stecher B 801 Berry D
Stecher B 801 Loy A
Stecher B 801 Wagner M
Stecher B 801 Eichorst SA
Stecher B 801 Mussmann M
Stecher B 801 Wasmund K
Stecher B 801 Herbold CW
Stecher B 801 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Mußmann M 1747 Horn M
Mußmann M 1747 Daims H
Mußmann M 1747 Berry D
Mußmann M 1747 Loy A
Mußmann M 1747 Wagner M
Mußmann M 1747 Eichorst SA
Mußmann M 1747 Mussmann M
Mußmann M 1747 Wasmund K
Mußmann M 1747 Herbold CW
Mußmann M 1747 Sedlacek CJ
Pjevac P 1297 Horn M
Pjevac P 1297 Daims H
Pjevac P 1297 Berry D
Pjevac P 1297 Loy A
Pjevac P 1297 Wagner M
Pjevac P 1297 Eichorst SA
Pjevac P 1297 Mussmann M
Pjevac P 1297 Wasmund K
Pjevac P 1297 Herbold CW
Pjevac P 1297 Sedlacek CJ
Krüger K 1748 Horn M
Krüger K 1748 Daims H
Krüger K 1748 Berry D
Krüger K 1748 Loy A
Krüger K 1748 Wagner M
Krüger K 1748 Eichorst SA
Krüger K 1748 Mussmann M
Krüger K 1748 Wasmund K
Krüger K 1748 Herbold CW
Krüger K 1748 Sedlacek CJ
Dyksma S 1749 Horn M
Dyksma S 1749 Daims H
Dyksma S 1749 Berry D
Dyksma S 1749 Loy A
Dyksma S 1749 Wagner M
Dyksma S 1749 Eichorst SA
Dyksma S 1749 Mussmann M
Dyksma S 1749 Wasmund K
Dyksma S 1749 Herbold CW
Dyksma S 1749 Sedlacek CJ
Pereira FC 1844 Horn M
Pereira FC 1844 Daims H
Pereira FC 1844 Berry D
Pereira FC 1844 Loy A
Pereira FC 1844 Wagner M
Pereira FC 1844 Eichorst SA
Pereira FC 1844 Mussmann M
Pereira FC 1844 Wasmund K
Pereira FC 1844 Herbold CW
Pereira FC 1844 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Hochkogler CM 1846 Horn M
Hochkogler CM 1846 Daims H
Hochkogler CM 1846 Berry D
Hochkogler CM 1846 Loy A
Hochkogler CM 1846 Wagner M
Hochkogler CM 1846 Eichorst SA
Hochkogler CM 1846 Mussmann M
Hochkogler CM 1846 Wasmund K
Hochkogler CM 1846 Herbold CW
Hochkogler CM 1846 Sedlacek CJ
Lieder B 1847 Horn M
Lieder B 1847 Daims H
Lieder B 1847 Berry D
Lieder B 1847 Loy A
Lieder B 1847 Wagner M
Lieder B 1847 Eichorst SA
Lieder B 1847 Mussmann M
Lieder B 1847 Wasmund K
Lieder B 1847 Herbold CW
Lieder B 1847 Sedlacek CJ
Rust P 1848 Horn M
Rust P 1848 Daims H
Rust P 1848 Berry D
Rust P 1848 Loy A
Rust P 1848 Wagner M
Rust P 1848 Eichorst SA
Rust P 1848 Mussmann M
Rust P 1848 Wasmund K
Rust P 1848 Herbold CW
Rust P 1848 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Meier SM 1849 Horn M
Meier SM 1849 Daims H
Meier SM 1849 Berry D
Meier SM 1849 Loy A
Meier SM 1849 Wagner M
Meier SM 1849 Eichorst SA
Meier SM 1849 Mussmann M
Meier SM 1849 Wasmund K
Meier SM 1849 Herbold CW
Meier SM 1849 Sedlacek CJ
Pignitter M 1850 Horn M
Pignitter M 1850 Daims H
Pignitter M 1850 Berry D
Pignitter M 1850 Loy A
Pignitter M 1850 Wagner M
Pignitter M 1850 Eichorst SA
Pignitter M 1850 Mussmann M
Pignitter M 1850 Wasmund K
Pignitter M 1850 Herbold CW
Pignitter M 1850 Sedlacek CJ
Riva A 1551 Horn M
Riva A 1551 Daims H
Riva A 1551 Berry D
Riva A 1551 Loy A
Riva A 1551 Wagner M
Riva A 1551 Eichorst SA
Riva A 1551 Mussmann M
Riva A 1551 Wasmund K
Riva A 1551 Herbold CW
Riva A 1551 Sedlacek CJ
Leitinger A 1851 Horn M
Leitinger A 1851 Daims H
Leitinger A 1851 Berry D
Leitinger A 1851 Loy A
Leitinger A 1851 Wagner M
Leitinger A 1851 Eichorst SA
Leitinger A 1851 Mussmann M
Leitinger A 1851 Wasmund K
Leitinger A 1851 Herbold CW
Leitinger A 1851 Sedlacek CJ
Bruk A 1852 Horn M
Bruk A 1852 Daims H
Bruk A 1852 Berry D
Bruk A 1852 Loy A
Bruk A 1852 Wagner M
Bruk A 1852 Eichorst SA
Bruk A 1852 Mussmann M
Bruk A 1852 Wasmund K
Bruk A 1852 Herbold CW
Bruk A 1852 Sedlacek CJ
Wagner S 1853 Horn M
Wagner S 1853 Daims H
Wagner S 1853 Berry D
Wagner S 1853 Loy A
Wagner S 1853 Wagner M
Wagner S 1853 Eichorst SA
Wagner S 1853 Mussmann M
Wagner S 1853 Wasmund K
Wagner S 1853 Herbold CW
Wagner S 1853 Sedlacek CJ
Hans J 1854 Horn M
Hans J 1854 Daims H
Hans J 1854 Berry D
Hans J 1854 Loy A
Hans J 1854 Wagner M
Hans J 1854 Eichorst SA
Hans J 1854 Mussmann M
Hans J 1854 Wasmund K
Hans J 1854 Herbold CW
Hans J 1854 Sedlacek CJ
Widder S 662 Horn M
Widder S 662 Daims H
Widder S 662 Berry D
Widder S 662 Loy A
Widder S 662 Wagner M
Widder S 662 Eichorst SA
Widder S 662 Mussmann M
Widder S 662 Wasmund K
Widder S 662 Herbold CW
Widder S 662 Sedlacek CJ
Ley JP 1855 Horn M
Ley JP 1855 Daims H
Ley JP 1855 Berry D
Ley JP 1855 Loy A
Ley JP 1855 Wagner M
Ley JP 1855 Eichorst SA
Ley JP 1855 Mussmann M
Ley JP 1855 Wasmund K
Ley JP 1855 Herbold CW
Ley JP 1855 Sedlacek CJ
Krammer GE 1856 Horn M
Krammer GE 1856 Daims H
Krammer GE 1856 Berry D
Krammer GE 1856 Loy A
Krammer GE 1856 Wagner M
Krammer GE 1856 Eichorst SA
Krammer GE 1856 Mussmann M
Krammer GE 1856 Wasmund K
Krammer GE 1856 Herbold CW
Krammer GE 1856 Sedlacek CJ
Somoza V 1857 Horn M
Somoza V 1857 Daims H
Somoza V 1857 Berry D
Somoza V 1857 Loy A
Somoza V 1857 Wagner M
Somoza V 1857 Eichorst SA
Somoza V 1857 Mussmann M
Somoza V 1857 Wasmund K
Somoza V 1857 Herbold CW
Somoza V 1857 Sedlacek CJ
Szabó G 700 Horn M
Szabó G 700 Daims H
Szabó G 700 Berry D
Szabó G 700 Loy A
Szabó G 700 Wagner M
Szabó G 700 Eichorst SA
Szabó G 700 Mussmann M
Szabó G 700 Wasmund K
Szabó G 700 Herbold CW
Szabó G 700 Sedlacek CJ
Schulz F 296 Horn M
Schulz F 296 Daims H
Schulz F 296 Berry D
Schulz F 296 Loy A
Schulz F 296 Wagner M
Schulz F 296 Eichorst SA
Schulz F 296 Mussmann M
Schulz F 296 Wasmund K
Schulz F 296 Herbold CW
Schulz F 296 Sedlacek CJ
Toenshoff ER 295 Horn M
Toenshoff ER 295 Daims H
Toenshoff ER 295 Berry D
Toenshoff ER 295 Loy A
Toenshoff ER 295 Wagner M
Toenshoff ER 295 Eichorst SA
Toenshoff ER 295 Mussmann M
Toenshoff ER 295 Wasmund K
Toenshoff ER 295 Herbold CW
Toenshoff ER 295 Sedlacek CJ
Volland JM 661 Horn M
Volland JM 661 Daims H
Volland JM 661 Berry D
Volland JM 661 Loy A
Volland JM 661 Wagner M
Volland JM 661 Eichorst SA
Volland JM 661 Mussmann M
Volland JM 661 Wasmund K
Volland JM 661 Herbold CW
Volland JM 661 Sedlacek CJ
Finkel OM 1709 Horn M
Finkel OM 1709 Daims H
Finkel OM 1709 Berry D
Finkel OM 1709 Loy A
Finkel OM 1709 Wagner M
Finkel OM 1709 Eichorst SA
Finkel OM 1709 Mussmann M
Finkel OM 1709 Wasmund K
Finkel OM 1709 Herbold CW
Finkel OM 1709 Sedlacek CJ
Belkin S 1710 Horn M
Belkin S 1710 Daims H
Belkin S 1710 Berry D
Belkin S 1710 Loy A
Belkin S 1710 Wagner M
Belkin S 1710 Eichorst SA
Belkin S 1710 Mussmann M
Belkin S 1710 Wasmund K
Belkin S 1710 Herbold CW
Belkin S 1710 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Manzano-Marín A 1744 Horn M
Manzano-Marín A 1744 Daims H
Manzano-Marín A 1744 Berry D
Manzano-Marín A 1744 Loy A
Manzano-Marín A 1744 Wagner M
Manzano-Marín A 1744 Eichorst SA
Manzano-Marín A 1744 Mussmann M
Manzano-Marín A 1744 Wasmund K
Manzano-Marín A 1744 Herbold CW
Manzano-Marín A 1744 Sedlacek CJ
Szabó G 700 Horn M
Szabó G 700 Daims H
Szabó G 700 Berry D
Szabó G 700 Loy A
Szabó G 700 Wagner M
Szabó G 700 Eichorst SA
Szabó G 700 Mussmann M
Szabó G 700 Wasmund K
Szabó G 700 Herbold CW
Szabó G 700 Sedlacek CJ
Simon JC 1745 Horn M
Simon JC 1745 Daims H
Simon JC 1745 Berry D
Simon JC 1745 Loy A
Simon JC 1745 Wagner M
Simon JC 1745 Eichorst SA
Simon JC 1745 Mussmann M
Simon JC 1745 Wasmund K
Simon JC 1745 Herbold CW
Simon JC 1745 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Latorre A 1746 Horn M
Latorre A 1746 Daims H
Latorre A 1746 Berry D
Latorre A 1746 Loy A
Latorre A 1746 Wagner M
Latorre A 1746 Eichorst SA
Latorre A 1746 Mussmann M
Latorre A 1746 Wasmund K
Latorre A 1746 Herbold CW
Latorre A 1746 Sedlacek CJ
Ponnudurai R 1356 Horn M
Ponnudurai R 1356 Daims H
Ponnudurai R 1356 Berry D
Ponnudurai R 1356 Loy A
Ponnudurai R 1356 Wagner M
Ponnudurai R 1356 Eichorst SA
Ponnudurai R 1356 Mussmann M
Ponnudurai R 1356 Wasmund K
Ponnudurai R 1356 Herbold CW
Ponnudurai R 1356 Sedlacek CJ
Kleiner M 1338 Horn M
Kleiner M 1338 Daims H
Kleiner M 1338 Berry D
Kleiner M 1338 Loy A
Kleiner M 1338 Wagner M
Kleiner M 1338 Eichorst SA
Kleiner M 1338 Mussmann M
Kleiner M 1338 Wasmund K
Kleiner M 1338 Herbold CW
Kleiner M 1338 Sedlacek CJ
Sayavedra L 1355 Horn M
Sayavedra L 1355 Daims H
Sayavedra L 1355 Berry D
Sayavedra L 1355 Loy A
Sayavedra L 1355 Wagner M
Sayavedra L 1355 Eichorst SA
Sayavedra L 1355 Mussmann M
Sayavedra L 1355 Wasmund K
Sayavedra L 1355 Herbold CW
Sayavedra L 1355 Sedlacek CJ
Petersen JM 1317 Horn M
Petersen JM 1317 Daims H
Petersen JM 1317 Berry D
Petersen JM 1317 Loy A
Petersen JM 1317 Wagner M
Petersen JM 1317 Eichorst SA
Petersen JM 1317 Mussmann M
Petersen JM 1317 Wasmund K
Petersen JM 1317 Herbold CW
Petersen JM 1317 Sedlacek CJ
Moche M 1720 Horn M
Moche M 1720 Daims H
Moche M 1720 Berry D
Moche M 1720 Loy A
Moche M 1720 Wagner M
Moche M 1720 Eichorst SA
Moche M 1720 Mussmann M
Moche M 1720 Wasmund K
Moche M 1720 Herbold CW
Moche M 1720 Sedlacek CJ
Otto A 1721 Horn M
Otto A 1721 Daims H
Otto A 1721 Berry D
Otto A 1721 Loy A
Otto A 1721 Wagner M
Otto A 1721 Eichorst SA
Otto A 1721 Mussmann M
Otto A 1721 Wasmund K
Otto A 1721 Herbold CW
Otto A 1721 Sedlacek CJ
Becher D 1364 Horn M
Becher D 1364 Daims H
Becher D 1364 Berry D
Becher D 1364 Loy A
Becher D 1364 Wagner M
Becher D 1364 Eichorst SA
Becher D 1364 Mussmann M
Becher D 1364 Wasmund K
Becher D 1364 Herbold CW
Becher D 1364 Sedlacek CJ
Takeuchi T 1722 Horn M
Takeuchi T 1722 Daims H
Takeuchi T 1722 Berry D
Takeuchi T 1722 Loy A
Takeuchi T 1722 Wagner M
Takeuchi T 1722 Eichorst SA
Takeuchi T 1722 Mussmann M
Takeuchi T 1722 Wasmund K
Takeuchi T 1722 Herbold CW
Takeuchi T 1722 Sedlacek CJ
Satoh N 1358 Horn M
Satoh N 1358 Daims H
Satoh N 1358 Berry D
Satoh N 1358 Loy A
Satoh N 1358 Wagner M
Satoh N 1358 Eichorst SA
Satoh N 1358 Mussmann M
Satoh N 1358 Wasmund K
Satoh N 1358 Herbold CW
Satoh N 1358 Sedlacek CJ
Dubilier N 900 Horn M
Dubilier N 900 Daims H
Dubilier N 900 Berry D
Dubilier N 900 Loy A
Dubilier N 900 Wagner M
Dubilier N 900 Eichorst SA
Dubilier N 900 Mussmann M
Dubilier N 900 Wasmund K
Dubilier N 900 Herbold CW
Dubilier N 900 Sedlacek CJ
Schweder T 1365 Horn M
Schweder T 1365 Daims H
Schweder T 1365 Berry D
Schweder T 1365 Loy A
Schweder T 1365 Wagner M
Schweder T 1365 Eichorst SA
Schweder T 1365 Mussmann M
Schweder T 1365 Wasmund K
Schweder T 1365 Herbold CW
Schweder T 1365 Sedlacek CJ
Markert S 1366 Horn M
Markert S 1366 Daims H
Markert S 1366 Berry D
Markert S 1366 Loy A
Markert S 1366 Wagner M
Markert S 1366 Eichorst SA
Markert S 1366 Mussmann M
Markert S 1366 Wasmund K
Markert S 1366 Herbold CW
Markert S 1366 Sedlacek CJ
Butler RN 1483 Horn M
Butler RN 1483 Daims H
Butler RN 1483 Berry D
Butler RN 1483 Loy A
Butler RN 1483 Wagner M
Butler RN 1483 Eichorst SA
Butler RN 1483 Mussmann M
Butler RN 1483 Wasmund K
Butler RN 1483 Herbold CW
Butler RN 1483 Sedlacek CJ
Kosek M 1484 Horn M
Kosek M 1484 Daims H
Kosek M 1484 Berry D
Kosek M 1484 Loy A
Kosek M 1484 Wagner M
Kosek M 1484 Eichorst SA
Kosek M 1484 Mussmann M
Kosek M 1484 Wasmund K
Kosek M 1484 Herbold CW
Kosek M 1484 Sedlacek CJ
Krebs N 1485 Horn M
Krebs N 1485 Daims H
Krebs N 1485 Berry D
Krebs N 1485 Loy A
Krebs N 1485 Wagner M
Krebs N 1485 Eichorst SA
Krebs N 1485 Mussmann M
Krebs N 1485 Wasmund K
Krebs N 1485 Herbold CW
Krebs N 1485 Sedlacek CJ
Loechl C 1486 Horn M
Loechl C 1486 Daims H
Loechl C 1486 Berry D
Loechl C 1486 Loy A
Loechl C 1486 Wagner M
Loechl C 1486 Eichorst SA
Loechl C 1486 Mussmann M
Loechl C 1486 Wasmund K
Loechl C 1486 Herbold CW
Loechl C 1486 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Owino V 1487 Horn M
Owino V 1487 Daims H
Owino V 1487 Berry D
Owino V 1487 Loy A
Owino V 1487 Wagner M
Owino V 1487 Eichorst SA
Owino V 1487 Mussmann M
Owino V 1487 Wasmund K
Owino V 1487 Herbold CW
Owino V 1487 Sedlacek CJ
Zimmermann M 1488 Horn M
Zimmermann M 1488 Daims H
Zimmermann M 1488 Berry D
Zimmermann M 1488 Loy A
Zimmermann M 1488 Wagner M
Zimmermann M 1488 Eichorst SA
Zimmermann M 1488 Mussmann M
Zimmermann M 1488 Wasmund K
Zimmermann M 1488 Herbold CW
Zimmermann M 1488 Sedlacek CJ
and Morrison DJ 1489 Horn M
and Morrison DJ 1489 Daims H
and Morrison DJ 1489 Berry D
and Morrison DJ 1489 Loy A
and Morrison DJ 1489 Wagner M
and Morrison DJ 1489 Eichorst SA
and Morrison DJ 1489 Mussmann M
and Morrison DJ 1489 Wasmund K
and Morrison DJ 1489 Herbold CW
and Morrison DJ 1489 Sedlacek CJ
Riva A 1551 Horn M
Riva A 1551 Daims H
Riva A 1551 Berry D
Riva A 1551 Loy A
Riva A 1551 Wagner M
Riva A 1551 Eichorst SA
Riva A 1551 Mussmann M
Riva A 1551 Wasmund K
Riva A 1551 Herbold CW
Riva A 1551 Sedlacek CJ
Borgo F 1552 Horn M
Borgo F 1552 Daims H
Borgo F 1552 Berry D
Borgo F 1552 Loy A
Borgo F 1552 Wagner M
Borgo F 1552 Eichorst SA
Borgo F 1552 Mussmann M
Borgo F 1552 Wasmund K
Borgo F 1552 Herbold CW
Borgo F 1552 Sedlacek CJ
Lassandro C 1553 Horn M
Lassandro C 1553 Daims H
Lassandro C 1553 Berry D
Lassandro C 1553 Loy A
Lassandro C 1553 Wagner M
Lassandro C 1553 Eichorst SA
Lassandro C 1553 Mussmann M
Lassandro C 1553 Wasmund K
Lassandro C 1553 Herbold CW
Lassandro C 1553 Sedlacek CJ
Verduci E 1554 Horn M
Verduci E 1554 Daims H
Verduci E 1554 Berry D
Verduci E 1554 Loy A
Verduci E 1554 Wagner M
Verduci E 1554 Eichorst SA
Verduci E 1554 Mussmann M
Verduci E 1554 Wasmund K
Verduci E 1554 Herbold CW
Verduci E 1554 Sedlacek CJ
Morace G 1555 Horn M
Morace G 1555 Daims H
Morace G 1555 Berry D
Morace G 1555 Loy A
Morace G 1555 Wagner M
Morace G 1555 Eichorst SA
Morace G 1555 Mussmann M
Morace G 1555 Wasmund K
Morace G 1555 Herbold CW
Morace G 1555 Sedlacek CJ
Borghi E 1556 Horn M
Borghi E 1556 Daims H
Borghi E 1556 Berry D
Borghi E 1556 Loy A
Borghi E 1556 Wagner M
Borghi E 1556 Eichorst SA
Borghi E 1556 Mussmann M
Borghi E 1556 Wasmund K
Borghi E 1556 Herbold CW
Borghi E 1556 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Publications | Microbial Ecology, University of Vienna

Publications

Publications in peer reviewed journals

34 Publications found
  • Astrobiology as a framework for investigating antibiotic susceptibility: a study of Halomonas hydrothermalis

    Harrison JP, Angel R, Cockell CS
    2017 - J R Soc Interface, 126: online only

    Abstract: 

    Physical and chemical boundaries for microbial multiplication on Earth are strongly influenced by interactions between environmental extremes. However, little is known about how interactions between multiple stress parameters affect the sensitivity of microorganisms to antibiotics. Here, we assessed how 12 distinct permutations of salinity, availability of an essential nutrient (iron) and atmospheric composition (aerobic or microaerobic) affect the susceptibility of a polyextremotolerant bacterium, Halomonas hydrothermalis, to ampicillin, kanamycin and ofloxacin. While salinity had a significant impact on sensitivity to all three antibiotics (as shown by turbidimetric analyses), the nature of this impact was modified by iron availability and the ambient gas composition, with differing effects observed for each compound. These two parameters were found to be of particular importance when considered in combination and, in the case of ampicillin, had a stronger combined influence on antibiotic tolerance than salinity. Our data show how investigating microbial responses to multiple extremes, which are more representative of natural habitats than single extremes, can improve our understanding of the effects of antimicrobial compounds and suggest how studies of habitability, motivated by the desire to map the limits of life, can be used to systematically assess the effectiveness of antibiotics.

  • Adaptability as the key to success for the ubiquitous marine nitrite oxidizer Nitrococcus

    Füssel J, Lücker S, Yilmaz P, Nowka B, van Kessel MAHJ, Bourceau P, Hach PF, Littmann S, Berg J, Spieck E, Daims H, Kuypers MMM, Lam P
    2017 - Sci Adv, 3: e1700807

    Abstract: 

    Nitrite-oxidizing bacteria (NOB) have conventionally been regarded as a highly specialized functional group responsible for the production of nitrate in the environment. However, recent culture-based studies suggest that they have the capacity to lead alternative lifestyles, but direct environmental evidence for the contribution of marine nitrite oxidizers to other processes has been lacking to date. We report on the alternative biogeochemical functions, worldwide distribution, and sometimes high abundance of the marine NOB Nitrococcus. These largely overlooked bacteria are capable of not only oxidizing nitrite but also reducing nitrate and producing nitrous oxide, an ozone-depleting agent and greenhouse gas. Furthermore, Nitrococcus can aerobically oxidize sulfide, thereby also engaging in the sulfur cycle. In the currently fast-changing global oceans, these findings highlight the potential functional switches these ubiquitous bacteria can perform in various biogeochemical cycles, each with distinct or even contrasting consequences.

  • Vitamin and Amino Acid Auxotrophy in Anaerobic Consortia Operating under Methanogenic Conditions.

    Hubalek V, Buck M, Tan B, Foght J, Wendeberg A, Berry D, Bertilsson S, Eiler A
    2017 - mSystems, 5: e00038-17

    Abstract: 

    Syntrophy among Archaea and Bacteria facilitates the anaerobic degradation of organic compounds to CH4 and CO2. Particularly during aliphatic and aromatic hydrocarbon mineralization, as in the case of crude oil reservoirs and petroleum-contaminated sediments, metabolic interactions between obligate mutualistic microbial partners are of central importance. Using micromanipulation combined with shotgun metagenomic approaches, we describe the genomes of complex consortia within short-chain alkane-degrading cultures operating under methanogenic conditions. Metabolic reconstruction revealed that only a small fraction of genes in the metagenome-assembled genomes encode the capacity for fermentation of alkanes facilitated by energy conservation linked to H2 metabolism. Instead, the presence of inferred lifestyles based on scavenging anabolic products and intermediate fermentation products derived from detrital biomass was a common feature. Additionally, inferred auxotrophy for vitamins and amino acids suggests that the hydrocarbon-degrading microbial assemblages are structured and maintained by multiple interactions beyond the canonical H2-producing and syntrophic alkane degrader-methanogen partnership. Compared to previous work, our report points to a higher order of complexity in microbial consortia engaged in anaerobic hydrocarbon transformation. IMPORTANCE Microbial interactions between Archaea and Bacteria mediate many important chemical transformations in the biosphere from degrading abundant polymers to synthesis of toxic compounds. Two of the most pressing issues in microbial interactions are how consortia are established and how we can modulate these microbial communities to express desirable functions. Here, we propose that public goods (i.e., metabolites of high energy demand in biosynthesis) facilitate energy conservation for life under energy-limited conditions and determine the assembly and function of the consortia. Our report suggests that an understanding of public good dynamics could result in new ways to improve microbial pollutant degradation in anaerobic systems.

  • Ammonia-oxidising archaea living at low pH: Insights from comparative genomics.

    Herbold CW, Lehtovirta-Morley LE, Jung MY, Jehmlich N, Hausmann B, Han P, Loy A, Pester M, Sayavedra-Soto LA, Rhee SK, Prosser JI, Nicol GW, Wagner M, Gubry-Rangin C
    2017 - Environ. Microbiol., 12: 4939-4952

    Abstract: 

    Obligate acidophilic members of the thaumarchaeotal genus Candidatus Nitrosotalea play an important role in nitrification in acidic soils, but their evolutionary and physiological adaptations to acidic environments are still poorly understood, with only a single member of this genus (Ca. N. devanaterra) having its genome sequenced. In this study, we sequenced the genomes of two additional cultured Ca. Nitrosotalea strains, extracted an almost complete Ca. Nitrosotalea metagenome-assembled genome from an acidic fen, and performed comparative genomics of the four Ca. Nitrosotalea genomes with 19 other archaeal ammonia oxidiser genomes. Average nucleotide and amino acid identities revealed that the four Ca. Nitrosotalea strains represent separate species within the genus. The four Ca. Nitrosotalea genomes contained a core set of 103 orthologous gene families absent from all other ammonia-oxidizing archaea and, for most of these gene families, expression could be demonstrated in laboratory culture or the environment via proteomic or metatranscriptomic analyses respectively. Phylogenetic analyses indicated that four of these core gene families were acquired by the Ca. Nitrosotalea common ancestor via horizontal gene transfer from acidophilic representatives of Euryarchaeota. We hypothesize that gene exchange with these acidophiles contributed to the competitive success of the Ca. Nitrosotalea lineage in acidic environments.

  • Abiotic Conversion of Extracellular NH2OH Contributes to N2O Emission during Ammonia Oxidation.

    Liu S, Han P, Hink L, Prosser JI, Wagner M, Brüggemann N
    2017 - Environ. Sci. Technol., 22: 13122-13132

    Abstract: 

    Abiotic processes involving the reactive ammonia-oxidation intermediates nitric oxide (NO) or hydroxylamine (NH2OH) for N2O production have been indicated recently. The latter process would require the availability of substantial amounts of free NH2OH for chemical reactions during ammonia (NH3) oxidation, but little is known about extracellular NH2OH formation by the different clades of ammonia-oxidizing microbes. Here we determined extracellular NH2OH concentrations in culture media of several ammonia-oxidizing bacteria (AOB) and archaea (AOA), as well as one complete ammonia oxidizer (comammox) enrichment (Ca. Nitrospira inopinata) during incubation under standard cultivation conditions. NH2OH was measurable in the incubation media of Nitrosomonas europaea, Nitrosospira multiformis, Nitrososphaera gargensis, and Ca. Nitrosotenuis uzonensis, but not in media of the other tested AOB and AOA. NH2OH was also formed by the comammox enrichment during NH3 oxidation. This enrichment exhibited the largest NH2OH:final product ratio (1.92%), followed by N. multiformis (0.56%) and N. gargensis (0.46%). The maximum proportions of NH4+ converted to N2O via extracellular NH2OH during incubation, estimated on the basis of NH2OH abiotic conversion rates, were 0.12%, 0.08%, and 0.14% for AOB, AOA, and Ca. Nitrospira inopinata, respectively, and were consistent with published NH4+:N2O conversion ratios for AOB and AOA.

  • Depth distribution and assembly of sulfate-reducing microbial communities in marine sediments of Aarhus Bay

    Jochum LM, Chena X, Lever MA, Loy A, Jørgensen BB, Schramm A, Kjeldsen KU
    2017 - Appl Environ Microbiol, 83: e01547-17

    Abstract: 

    Most sulfate-reducing microorganisms (SRM) present in subsurface marine sediments belong to uncultured groups only distantly related to known SRM and it remains unclear how changing geochemical zones and sediment depth influence their community structure. We mapped the community composition and abundance of SRM by amplicon-sequencing and quantifying dsrB, which encodes dissimilatory sulfite reductase subunit beta, in sediment samples covering different vertical geochemical zones ranging from the surface sediment to the deep sulfate-depleted subsurface at four locations in Aarhus Bay, Denmark. SRM were present in all geochemical zones including sulfate-depleted methanogenic sediment. The biggest shift in SRM community composition and abundance occurring across the transition from bioturbated surface sediments into non-bioturbated sediments below, where redox fluctuations and input of fresh organic matter due to macrofaunal activity are absent. SRM abundance correlated with sulfate reduction rates determined for the same sediments. Sulfate availability showed weaker correlation with SRM abundances and no significant correlation with the composition of the SRM community. The overall SRM species diversity decreased with depth, yet we identified a subset of highly abundant community members that persists across all vertical geochemical zones of all stations. We conclude that subsurface SRM communities assemble by persistence of members of the surface community and that the transition from the bioturbated surface sediment to the unmixed sediment below is a main site of assembly of the subsurface SRM community.

  • Bottled aqua incognita: Microbiota assembly and dissolved organic matter diversity in natural mineral waters

    Lesaulnier CC, Herbold CW, Pelikan C, Gérard C, Le Coz X, Gagnot S, Berry D, Niggemann J, Dittmar T, Singer GA, Loy A
    2017 - Microbiome, 5: 126

    Abstract: 

    Background: Non-carbonated natural mineral waters contain microorganisms that regularly grow after bottling despite low concentrations of dissolved organic matter (DOM). Yet, the compositions of bottled water microbiota and organic substrates that fuel microbial activity, and how both change after bottling, are still largely unknown.

    Results: We performed a multifaceted analysis of microbiota and DOM diversity in twelve natural mineral waters from six European countries. 16S rRNA gene-based analyses showed that less than ten species-level operational taxonomic units (OTUs) dominated the bacterial communities in the water phase and associated with the bottle wall after a short phase of post-bottling growth. Members of the betaproteobacterial genera Curvibacter, Aquabacterium, and Polaromonas (Comamonadaceae) grew in most waters and represent ubiquitous, mesophilic, heterotrophic aerobes in bottled waters. Ultrahigh-resolution mass spectrometry of DOM in bottled waters and their corresponding source waters identified thousands of molecular formulae characteristic of mostly refractory, soil-derived DOM.

    Conclusions. The bottle environment, including source water physicochemistry, selected for growth of a similar low-diversity microbiota across various bottled waters. Relative abundance changes of hundreds of multi-carbon molecules were related to growth of less than ten abundant OTUs. We thus speculate that individual bacteria cope with oligotrophic conditions by simultaneously consuming diverse DOM molecules.

  • Identification of secondary metabolite gene clusters in the Pseudovibrio genus reveals encouraging biosynthetic potential toward the production of novel bioactive compounds

    Naughton LM, Romano S, O'Gara F, Dobson ADW
    2017 - Front Microbiol, 8: 1494

    Abstract: 

    Increased incidences of antimicrobial resistance and the emergence of pan-resistant 'superbugs' have provoked an extreme sense of urgency amongst researchers focusing on the discovery of potentially novel antimicrobial compounds. A strategic shift in focus from the terrestrial to the marine environment has resulted in the discovery of a wide variety of structurally and functionally diverse bioactive compounds from numerous marine sources, including sponges. Bacteria found in close association with sponges and other marine invertebrates have recently gained much attention as potential sources of many of these novel bioactive compounds. Members of the genus Pseudovibrio are one such group of organisms. In this study, we interrogate the genomes of 21 Pseudovibrio strains isolated from a variety of marine sources, for the presence, diversity and distribution of biosynthetic gene clusters (BGCs). We expand on results obtained from antiSMASH analysis to demonstrate the similarity between the Pseudovibrio-related BGCs and those characterized in other bacteria and corroborate our findings with phylogenetic analysis. We assess how domain organization of the most abundant type of BGCs present among the isolates (Non-ribosomal peptide synthetases and Polyketide synthases) may influence the diversity of compounds produced by these organisms and highlight for the first time the potential for novel compound production from this genus of bacteria, using a genome guided approach.

  • Kinetic analysis of a complete nitrifier reveals an oligotrophic lifestyle.

    Kits KD, Sedlacek CJ, Lebedeva EV, Han P, Bulaev A, Pjevac P, Daebeler A, Romano S, Albertsen M, Stein LY, Daims H, Wagner M
    2017 - Nature, 549: 269-272

    Abstract: 

    Nitrification, the oxidation of ammonia (NH3) via nitrite (NO2(-)) to nitrate (NO3(-)), is a key process of the biogeochemical nitrogen cycle. For decades, ammonia and nitrite oxidation were thought to be separately catalysed by ammonia-oxidizing bacteria (AOB) and archaea (AOA), and by nitrite-oxidizing bacteria (NOB). The recent discovery of complete ammonia oxidizers (comammox) in the NOB genus Nitrospira, which alone convert ammonia to nitrate, raised questions about the ecological niches in which comammox Nitrospira successfully compete with canonical nitrifiers. Here we isolate a pure culture of a comammox bacterium, Nitrospira inopinata, and show that it is adapted to slow growth in oligotrophic and dynamic habitats on the basis of a high affinity for ammonia, low maximum rate of ammonia oxidation, high growth yield compared to canonical nitrifiers, and genomic potential for alternative metabolisms. The nitrification kinetics of four AOA from soil and hot springs were determined for comparison. Their surprisingly poor substrate affinities and lower growth yields reveal that, in contrast to earlier assumptions, AOA are not necessarily the most competitive ammonia oxidizers present in strongly oligotrophic environments and that N. inopinata has the highest substrate affinity of all analysed ammonia oxidizer isolates except the marine AOA Nitrosopumilus maritimus SCM1 (ref. 3). These results suggest a role for comammox organisms in nitrification under oligotrophic and dynamic conditions.

  • AmoA-targeted polymerase chain reaction primers for the specific detection and quantification of comammox Nitrospira in the environment

    Pjevac P, Schauberger C, Poghosyan L, Herbold CW, van Kessel MAHJ, Daebeler A, Steinberger M, Jetten MSM, Luecker S, Wagner M, Daims H
    2017 - Front Microbiol, 8:1508
    Comammox PCR primers

    Abstract: 

    Nitrification, the oxidation of ammonia via nitrite to nitrate, has always been considered to be catalyzed by the concerted activity of ammonia- and nitrite-oxidizing microorganisms. Only recently, complete ammonia oxidizers (‘comammox’), which oxidize ammonia to nitrate on their own, were identified in the bacterial genus Nitrospira, previously assumed to contain only canonical nitrite oxidizers. Nitrospira are widespread in nature, but for assessments of the distribution and functional importance of comammox Nitrospira in ecosystems, cultivation-independent tools to distinguish comammox from strictly nitrite oxidizing Nitrospira are required. Here we developed new PCR primer sets that specifically target the amoA genes coding for subunit A of the distinct ammonia monooxygenase of comammox Nitrospira. While existing primers capture only a fraction of the known comammox amoA diversity, the new primer sets cover as much as 95% of the comammox amoA clade A and 92% of the clade B sequences in a reference database containing 326 comammox amoA genes with sequence information at the primer binding sites. Application of the primers to 13 samples from engineered systems (a groundwater well, drinking water treatment and wastewater treatment plants) and other habitats (rice paddy and forest soils, rice rhizosphere, brackish lake sediment and freshwater biofilm) detected comammox Nitrospira in all samples and revealed a considerable diversity of comammox in most habitats. Excellent primer specificity for comammox amoA was achieved by avoiding the use of highly degenerate primer preparations and by using equimolar mixtures of oligonucleotides that match existing comammox amoA genes. Quantitative PCR with these equimolar primer mixtures was highly sensitive and specific, and enabled the efficient quantification of clade A and clade B comammox amoA gene copy numbers in environmental samples. The measured relative abundances of comammox Nitrospira, compared to canonical ammonia oxidizers, were highly variable across environments. The new comammox amoA-targeted primers enable more encompassing future studies of nitrifying microorganisms in diverse habitats. For example, they may be used to monitor the population dynamics of uncultured comammox organisms under changing environmental conditions and in response to altered treatments in engineered and agricultural ecosystems.

  • In situ architecture, function, and evolution of a contractile injection system.

    Böck D, Medeiros JM, Tsao HF, Penz T, Weiss GL, Aistleitner K, Horn M, Pilhofer M
    2017 - Science, 6352: 713-717

    Abstract: 

    Contractile injection systems mediate bacterial cell-cell interactions by a bacteriophage tail-like structure. In contrast to extracellular systems, the type 6 secretion system (T6SS) is defined by intracellular localization and attachment to the cytoplasmic membrane. Here we used cryo-focused ion beam milling, electron cryotomography, and functional assays to study a T6SS in Amoebophilus asiaticus The in situ architecture revealed three modules, including a contractile sheath-tube, a baseplate, and an anchor. All modules showed conformational changes upon firing. Lateral baseplate interactions coordinated T6SSs in hexagonal arrays. The system mediated interactions with host membranes and may participate in phagosome escape. Evolutionary sequence analyses predicted that T6SSs are more widespread than previously thought. Our insights form the basis for understanding T6SS key concepts and exploring T6SS diversity.

  • Allspice and Clove As Source of Triterpene Acids Activating the G Protein-Coupled Bile Acid Receptor TGR5.

    Ladurner A, Zehl M, Grienke U, Hofstadler C, Faur N, Pereira FC, Berry D, Dirsch VM, Rollinger JM
    2017 - Front Pharmacol, 8: 468

    Abstract: 

    Worldwide, metabolic diseases such as obesity and type 2 diabetes have reached epidemic proportions. A major regulator of metabolic processes that gained interest in recent years is the bile acid receptor TGR5 (Takeda G protein-coupled receptor 5). This G protein-coupled membrane receptor can be found predominantly in the intestine, where it is mainly responsible for the secretion of the incretins glucagon-like peptide 1 (GLP-1) and peptide YY (PYY). The aim of this study was (i) to identify plant extracts with TGR5-activating potential, (ii) to narrow down their activity to the responsible constituents, and (iii) to assess whether the intestinal microbiota produces transformed metabolites with a different activity profile. Chenodeoxycholic acid (CDCA) served as positive control for both, the applied cell-based luciferase reporter gene assay for TGR5 activity and the biotransformation assay using mouse fecal slurry. The suitability of the workflow was demonstrated by the biotransformation of CDCA to lithocholic acid resulting in a distinct increase in TGR5 activity. Based on a traditional Tibetan formula, 19 plant extracts were selected and investigated for TGR5 activation. Extracts from the commonly used spices Syzygium aromaticum (SaroE, clove), Pimenta dioica (PdioE, allspice), and Kaempferia galanga (KgalE, aromatic ginger) significantly increased TGR5 activity. After biotransformation, only KgalE showed significant differences in its metabolite profile, which, however, did not alter its TGR5 activity compared to non-transformed KgalE. UHPLC-HRMS (high-resolution mass spectrometry) analysis revealed triterpene acids (TTAs) as the main constituents of the extracts SaroE and PdioE. Identification and quantification of TTAs in these two extracts as well as comparison of their TGR5 activity with reconstituted TTA mixtures allowed the attribution of the TGR5 activity to TTAs. EC50s were determined for the main TTAs, i.e., oleanolic acid (2.2 ± 1.6 μM), ursolic acid (1.1 ± 0.2 μM), as well as for the hitherto unknown TGR5 activators corosolic acid (0.5 ± 1.0 μM) and maslinic acid (3.7 ± 0.7 μM). In conclusion, extracts of clove, allspice, and aromatic ginger activate TGR5, which might play a pivotal role in their therapeutic use for the treatment of metabolic diseases. Moreover, the TGR5 activation of SaroE and PdioE could be pinpointed solely to TTAs.

  • Unexpected genomic features in widespread intracellular bacteria: evidence for motility of marine chlamydiae.

    Collingro A, Köstlbacher S, Mussmann M, Stepanauskas R, Hallam SJ, Horn M
    2017 - ISME J, 10: 2334-2344

    Abstract: 

    Chlamydiae are obligate intracellular bacteria comprising important human pathogens and symbionts of protists. Molecular evidence indicates a tremendous diversity of chlamydiae particularly in marine environments, yet our current knowledge is based mainly on terrestrial representatives. Here we provide first insights into the biology of marine chlamydiae representing three divergent clades. Our analysis of single-cell amplified genomes revealed hallmarks of the chlamydial lifestyle, supporting the ancient origin of their characteristic developmental cycle and major virulence mechanisms. Surprisingly, these chlamydial genomes encode a complete flagellar apparatus, a previously unreported feature. We show that flagella are an ancient trait that was subject to differential gene loss among extant chlamydiae. Together with a chemotaxis system, these marine chlamydiae are likely motile, with flagella potentially playing a role during host cell infection. This study broadens our view on chlamydial biology and indicates a largely underestimated potential to adapt to different hosts and environments.

  • 'Candidatus Cochliophilus cryoturris' (Coxiellaceae), a symbiont of the testate amoeba Cochliopodium minus.

    Tsao HF, Scheikl U, Volland JM, Köhsler M, Bright M, Walochnik J, Horn M
    2017 - Sci Rep, 1: 3394

    Abstract: 

    Free-living amoebae are well known for their role in controlling microbial community composition through grazing, but some groups, namely Acanthamoeba species, also frequently serve as hosts for bacterial symbionts. Here we report the first identification of a bacterial symbiont in the testate amoeba Cochliopodium. The amoeba was isolated from a cooling tower water sample and identified as C. minus. Fluorescence in situ hybridization and transmission electron microscopy revealed intracellular symbionts located in vacuoles. 16S rRNA-based phylogenetic analysis identified the endosymbiont as member of a monophyletic group within the family Coxiellaceae (Gammaprotebacteria; Legionellales), only moderately related to known amoeba symbionts. We propose to tentatively classify these bacteria as 'Candidatus Cochliophilus cryoturris'. Our findings add both, a novel group of amoeba and a novel group of symbionts, to the growing list of bacteria-amoeba relationships.

  • Biphasic Metabolism and Host Interaction of a Chlamydial Symbiont.

    König L, Siegl A, Penz T, Haider S, Wentrup C, Polzin J, Mann E, Schmitz-Esser S, Domman D, Horn M
    2017 - mSystems, 2: e00202-16

    Abstract: 

    Chlamydiae are obligate intracellular bacteria comprising well-known human pathogens and ubiquitous symbionts of protists, which are characterized by a unique developmental cycle. Here we comprehensively analyzed gene expression dynamics of Protochlamydia amoebophila during infection of its Acanthamoeba host by RNA sequencing. This revealed a highly dynamic transcriptional landscape, where major transcriptional shifts are conserved among chlamydial symbionts and pathogens. Our data served to propose a time-resolved model for type III protein secretion during the developmental cycle, and we provide evidence for a biphasic metabolism of P. amoebophila during infection, which involves energy parasitism and amino acids as the carbon source during initial stages and a postreplicative switch to endogenous glucose-based ATP production. This fits well with major transcriptional changes in the amoeba host, where upregulation of complex sugar breakdown precedes the P. amoebophila metabolic switch. The biphasic chlamydial metabolism represents a unique adaptation to exploit eukaryotic host cells, which likely contributed to the evolutionary success of this group of microbes. IMPORTANCE Chlamydiae are known as major bacterial pathogens of humans, causing the ancient disease trachoma, but they are also frequently found in the environment where they infect ubiquitous protists such as amoebae. All known chlamydiae require a eukaryotic host cell to thrive. Using the environmental chlamydia Protochlamydia amoebophila within its natural host, Acanthamoeba castellanii, we investigated gene expression dynamics in vivo and throughout the complete chlamydial developmental cycle for the first time. This allowed us to infer how a major virulence mechanism, the type III secretion system, is regulated and employed, and we show that the physiology of chlamydiae undergoes a complete shift regarding carbon metabolism and energy generation. This study provides comprehensive insights into the infection strategy of chlamydiae and reveals a unique adaptation to life within a eukaryotic host cell.

  • Crenothrix are major methane consumers in stratified lakes.

    Oswald K, Graf JS, Littmann S, Tienken D, Brand A, Wehrli B, Albertsen M, Daims H, Wagner M, Kuypers MM, Schubert CJ, Milucka J
    2017 - ISME J, 9: 2124-2140
    Crenothrix

    Abstract: 

    Methane-oxidizing bacteria represent a major biological sink for methane and are thus Earth's natural protection against this potent greenhouse gas. Here we show that in two stratified freshwater lakes a substantial part of upward-diffusing methane was oxidized by filamentous gamma-proteobacteria related to Crenothrix polyspora. These filamentous bacteria have been known as contaminants of drinking water supplies since 1870, but their role in the environmental methane removal has remained unclear. While oxidizing methane, these organisms were assigned an 'unusual' methane monooxygenase (MMO), which was only distantly related to 'classical' MMO of gamma-proteobacterial methanotrophs. We now correct this assignment and show that Crenothrix encode a typical gamma-proteobacterial PmoA. Stable isotope labeling in combination swith single-cell imaging mass spectrometry revealed methane-dependent growth of the lacustrine Crenothrix with oxygen as well as under oxygen-deficient conditions. Crenothrix genomes encoded pathways for the respiration of oxygen as well as for the reduction of nitrate to N2O. The observed abundance and planktonic growth of Crenothrix suggest that these methanotrophs can act as a relevant biological sink for methane in stratified lakes and should be considered in the context of environmental removal of methane.

  • Capturing the genetic makeup of the active microbiome in situ.

    Singer E, Wagner M, Woyke T
    2017 - ISME J, 9: 1949-1963

    Abstract: 

    More than any other technology, nucleic acid sequencing has enabled microbial ecology studies to be complemented with the data volumes necessary to capture the extent of microbial diversity and dynamics in a wide range of environments. In order to truly understand and predict environmental processes, however, the distinction between active, inactive and dead microbial cells is critical. Also, experimental designs need to be sensitive toward varying population complexity and activity, and temporal as well as spatial scales of process rates. There are a number of approaches, including single-cell techniques, which were designed to study in situ microbial activity and that have been successively coupled to nucleic acid sequencing. The exciting new discoveries regarding in situ microbial activity provide evidence that future microbial ecology studies will indispensably rely on techniques that specifically capture members of the microbiome active in the environment. Herein, we review those currently used activity-based approaches that can be directly linked to shotgun nucleic acid sequencing, evaluate their relevance to ecology studies, and discuss future directions.

  • Evaluating the Detection of Hydrocarbon-Degrading Bacteria in 16S rRNA Gene Sequencing Surveys.

    Berry D, Gutierrez T
    2017 - Front Microbiol, 8: 2460

    Abstract: 

    Hydrocarbonoclastic bacteria (HCB) play a key role in the biodegradation of oil hydrocarbons in marine and other environments. A small number of taxa have been identified as obligate HCB, notably the Gammaproteobacterial genera Alcanivorax, Cycloclasticus, Marinobacter, Neptumonas, Oleiphilus, Oleispira, and Thalassolituus, as well as the Alphaproteobacterial genus Thalassospira. Detection of HCB in amplicon-based sequencing surveys relies on high coverage by PCR primers and accurate taxonomic classification. In this study, we performed a phylogenetic analysis to identify 16S rRNA gene sequence regions that represent the breadth of sequence diversity within these taxa. Using validated sequences, we evaluated 449 universal 16S rRNA gene-targeted bacterial PCR primer pairs for their coverage of these taxa. The results of this analysis provide a practical framework for selection of suitable primer sets for optimal detection of HCB in sequencing surveys.

  • Vibrational Spectroscopy for Imaging Single Microbial Cells in Complex Biological Samples.

    Harrison JP, Berry D
    2017 - Front Microbiol, 8: 675

    Abstract: 

    Vibrational spectroscopy is increasingly used for the rapid and non-destructive imaging of environmental and medical samples. Both Raman and Fourier-transform infrared (FT-IR) imaging have been applied to obtain detailed information on the chemical composition of biological materials, ranging from single microbial cells to tissues. Due to its compatibility with methods such as stable isotope labeling for the monitoring of cellular activities, vibrational spectroscopy also holds considerable power as a tool in microbial ecology. Chemical imaging of undisturbed biological systems (such as live cells in their native habitats) presents unique challenges due to the physical and chemical complexity of the samples, potential for spectral interference, and frequent need for real-time measurements. This Mini Review provides a critical synthesis of recent applications of Raman and FT-IR spectroscopy for characterizing complex biological samples, with a focus on developments in single-cell imaging. We also discuss how new spectroscopic methods could be used to overcome current limitations of single-cell analyses. Given the inherent complementarity of Raman and FT-IR spectroscopic methods, we discuss how combining these approaches could enable us to obtain new insights into biological activities either in situ or under conditions that simulate selected properties of the natural environment.

  • HuR small-molecule inhibitor elicits differential effects in adenomatosis polyposis and colorectal carcinogenesis

    Lang M, Berry D, Passecker K, Mesteri I, Bhuju S, Ebner F, Sedlyarov V, Evstatiev R, Dammann K, Loy A, Kuzyk O, Kovarik P, Khare V, Beibel M, Roma G, Meisner-Kober N, Gasche C
    2017 - Cancer Res., 77: 2424-2438

    Abstract: 

    HuR is an RNA-binding protein implicated in immune homeostasis and various cancers, including colorectal cancer. HuR binding to AU-rich elements within the 3' untranslated region of mRNAs encoding oncogenes, growth factors, and various cytokines leads message stability and translation. In this study, we evaluated HuR as a small-molecule target for preventing colorectal cancer in high-risk groups such as those with familial adenomatosis polyposis (FAP) or inflammatory bowel disease (IBD). In human specimens, levels of cytoplasmic HuR were increased in colonic epithelial cells from patients with IBD, IBD-cancer, FAP-adenoma, and colorectal cancer, but not in patients with IBD-dysplasia. Intraperitoneal injection of the HuR small-molecule inhibitor MS-444 in AOM/DSS mice, a model of IBD and inflammatory colon cancer, augmented DSS-induced weight loss and increased tumor multiplicity, size, and invasiveness. MS-444 treatment also abrogated tumor cell apoptosis and depleted tumor-associated eosinophils, accompanied by a decrease in IL18 and eotaxin-1. In contrast, HuR inhibition in APCMin mice, a model of FAP and colon cancer, diminished the number of small intestinal tumors generated. In this setting, fecal microbiota, evaluated by 16S rRNA gene amplicon sequencing, shifted to a state of reduced bacterial diversity, with an increased representation of Prevotella, Akkermansia, and Lachnospiraceae Taken together, our results indicate that HuR activation is an early event in FAP-adenoma but is not present in IBD-dysplasia. Furthermore, our results offer a preclinical proof of concept for HuR inhibition as an effective means of FAP chemoprevention, with caution advised in the setting of IBD.

  • The life sulfuric: Microbial ecology of sulfur cycling in marine sediments.

    Wasmund K, Mußmann M, Loy A
    2017 - Environ Microbiol Rep, 9: 323-344

    Abstract: 

    Almost the entire seafloor is covered with sediments that can be more than 10,000 m thick and represent a vast microbial ecosystem that is a major component of Earth's element and energy cycles. Notably, a significant proportion of microbial life in marine sediments can exploit energy conserved during transformations of sulfur compounds among different redox states. Sulfur cycling, which is primarily driven by sulfate reduction, is tightly interwoven with other important element cycles (carbon, nitrogen, iron, manganese) and therefore has profound implications for both cellular- and ecosystem-level processes. Sulfur-transforming microorganisms have evolved diverse genetic, metabolic, and in some cases, peculiar phenotypic features to fill an array of ecological niches in marine sediments. Here, we review recent and selected findings on the microbial guilds that are involved in the transformation of different sulfur compounds in marine sediments and emphasize how these are interlinked and have a major influence on ecology and biogeochemistry in the seafloor. Extraordinary discoveries have increased our knowledge on microbial sulfur cycling, mainly in sulfate-rich surface sediments, yet many questions remain regarding how sulfur redox processes may sustain the deep-subsurface biosphere and the impact of organic sulfur compounds on the marine sulfur cycle. This article is protected by copyright. All rights reserved.

  • Giant viruses with an expanded complement of translation system components.

    Schulz F, Yutin N, Ivanova NN, Ortega DR, Lee TK, Vierheilig J, Daims H, Horn M, Wagner M, Jensen GJ, Kyrpides NC, Koonin EV, Woyke T
    2017 - Science, 6333: 82-85
    Klosneuvirus

    Abstract: 

    The discovery of giant viruses blurred the sharp division between viruses and cellular life. Giant virus genomes encode proteins considered as signatures of cellular organisms, particularly translation system components, prompting hypotheses that these viruses derived from a fourth domain of cellular life. Here we report the discovery of a group of giant viruses (Klosneuviruses) in metagenomic data. Compared with other giant viruses, the Klosneuviruses encode an expanded translation machinery, including aminoacyl transfer RNA synthetases with specificities for all 20 amino acids. Notwithstanding the prevalence of translation system components, comprehensive phylogenomic analysis of these genes indicates that Klosneuviruses did not evolve from a cellular ancestor but rather are derived from a much smaller virus through extensive gain of host genes.

  • Members of the Oral Microbiota Are Associated with IL-8 Release by Gingival Epithelial Cells in Healthy Individuals.

    Schueller K, Riva A, Pfeiffer S, Berry D, Somoza V
    2017 - Front Microbiol, 8: 416

    Abstract: 

    The triggers for the onset of oral diseases are still poorly understood. The aim of this study was to characterize the oral bacterial community in healthy humans and its association with nutrition, oral hygiene habits, and the release of the inflammatory marker IL-8 from gingival epithelial cells (GECs) with and without stimulation by bacterial endotoxins to identify possible indicator operational taxonomic units (OTUs) associated with inflammatory marker status. GECs from 21 healthy participants (13 females, 8 males) were incubated with or without addition of bacterial lipopolysaccharides (LPSs), and the oral microbiota was profiled using 16S rRNA gene-targeted sequencing. The basal IL-8 release after 6 h was between 9.9 and 98.2 pg/ml, and bacterial communities were characteristic for healthy oral microbiota. The composition of the oral microbiota was associated with basal IL-8 levels, the intake of meat, tea, white wine, sweets and the use of chewing gum, as well as flossing habits, allergies, gender and body mass index. Additionally, eight OTUs were associated with high basal levels of IL-8 and GEC response to LPS, with high basal levels of IL-8, and 1 with low basal levels of IL8. The identification of indicator bacteria in healthy subjects with high levels of IL-8 release is of importance as they may be promising early warning indicators for the possible onset of oral diseases.

  • Variant profiling of evolving prokaryotic populations.

    Zojer M, Schuster LN, Schulz F, Pfundner A, Horn M, Rattei T
    2017 - PeerJ, e2997

    Abstract: 

    Genomic heterogeneity of bacterial species is observed and studied in experimental evolution experiments and clinical diagnostics, and occurs as micro-diversity of natural habitats. The challenge for genome research is to accurately capture this heterogeneity with the currently used short sequencing reads. Recent advances in NGS technologies improved the speed and coverage and thus allowed for deep sequencing of bacterial populations. This facilitates the quantitative assessment of genomic heterogeneity, including low frequency alleles or haplotypes. However, false positive variant predictions due to sequencing errors and mapping artifacts of short reads need to be prevented. We therefore created VarCap, a workflow for the reliable prediction of different types of variants even at low frequencies. In order to predict SNPs, InDels and structural variations, we evaluated the sensitivity and accuracy of different software tools using synthetic read data. The results suggested that the best sensitivity could be reached by a union of different tools, however at the price of increased false positives. We identified possible reasons for false predictions and used this knowledge to improve the accuracy by post-filtering the predicted variants according to properties such as frequency, coverage, genomic environment/localization and co-localization with other variants. We observed that best precision was achieved by using an intersection of at least two tools per variant. This resulted in the reliable prediction of variants above a minimum relative abundance of 2%. VarCap is designed for being routinely used within experimental evolution experiments or for clinical diagnostics. The detected variants are reported as frequencies within a VCF file and as a graphical overview of the distribution of the different variant/allele/haplotype frequencies. The source code of VarCap is available at https://github.com/ma2o/VarCap. In order to provide this workflow to a broad community, we implemeted VarCap on a Galaxy webserver, which is accessible at http://galaxy.csb.univie.ac.at.

  • Cultivation and characterization of Candidatus Nitrosocosmicus exaquare, an ammonia-oxidizing archaeon from a municipal wastewater treatment system.

    Sauder LA, Albertsen M, Engel K, Schwarz J, Nielsen PH, Wagner M, Neufeld JD
    2017 - ISME J, 5: 1142-1157
    Nitrosocosmicus

    Abstract: 

    Thaumarchaeota have been detected in several industrial and municipal wastewater treatment plants (WWTPs), despite the fact that ammonia-oxidizing archaea (AOA) are thought to be adapted to low ammonia environments. However, the activity, physiology and metabolism of WWTP-associated AOA remain poorly understood. We report the cultivation and complete genome sequence of Candidatus Nitrosocosmicus exaquare, a novel AOA representative from a municipal WWTP in Guelph, Ontario (Canada). In enrichment culture, Ca. N. exaquare oxidizes ammonia to nitrite stoichiometrically, is mesophilic, and tolerates at least 15 mm of ammonium chloride or sodium nitrite. Microautoradiography (MAR) for enrichment cultures demonstrates that Ca. N. exaquare assimilates bicarbonate in association with ammonia oxidation. However, despite using inorganic carbon, the ammonia-oxidizing activity of Ca. N. exaquare is greatly stimulated in enrichment culture by the addition of organic compounds, especially malate and succinate. Ca. N. exaquare cells are coccoid with a diameter of ~1-2 μm. Phylogenetically, Ca. N. exaquare belongs to the Nitrososphaera sister cluster within the Group I.1b Thaumarchaeota, a lineage which includes most other reported AOA sequences from municipal and industrial WWTPs. The 2.99 Mbp genome of Ca. N. exaquare encodes pathways for ammonia oxidation, bicarbonate fixation, and urea transport and breakdown. In addition, this genome encodes several key genes for dealing with oxidative stress, including peroxidase and catalase. Incubations of WWTP biofilm demonstrate partial inhibition of ammonia-oxidizing activity by 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (PTIO), suggesting that Ca. N. exaquare-like AOA may contribute to nitrification in situ. However, CARD-FISH-MAR showed no incorporation of bicarbonate by detected Thaumarchaeaota, suggesting that detected AOA may incorporate non-bicarbonate carbon sources or rely on an alternative and yet unknown metabolism.

  • Lifestyle and horizontal gene transfer-mediated evolution of Mucispirillum schaedleri, a core member of the murine gut microbiota

    Loy A, Pfann C, Steinberger M, Hanson B, Herp S, Brugiroux S, Gomes Neto JC, Boekschoten MV, Schwab C, Urich T, Ramer-Tait AE, Rattei T, Stecher B, Berry D
    2017 - mSystems, 2: e00171-16

    Abstract: 

    Mucispirillum schaedleri is an abundant inhabitant of the intestinal mucus layer of rodents and other animals and has been suggested to be a pathobiont, a commensal that plays a role in disease. In order to gain insights into its lifestyle, we analyzed the genome and transcriptome of M. schaedleri ASF 457 and performed physiological experiments to test traits predicted by its genome. Although described as a mucus inhabitant, M. schaedleri has limited capacity for degrading host-derived mucosal glycans and other complex polysaccharides. Additionally, M. schaedleri reduces nitrate and expresses systems for scavenging oxygen and reactive oxygen species in vivo, which may account for its localization close to the mucosal tissue and expansion during inflammation. Also of note, M. schaedleri harbors a type VI secretion system and putative effector proteins and can modify gene expression in mucosal tissue, suggesting intimate interactions with its host and a possible role in inflammation. The M. schaedleri genome has been shaped by extensive horizontal gene transfer, primarily from intestinal Epsilon- and Deltaproteobacteria, indicating that horizontal gene transfer has played a key role in defining its niche in the gut ecosystem.

  • Genomic repertoire of the Woeseiaceae/JTB255, cosmopolitan and abundant core members of microbial communities in marine sediments.

    Mußmann M, Pjevac P, Krüger K, Dyksma S
    2017 - ISME J, 5: 1276-1281

    Abstract: 

    To date, very little is known about the bacterial core community of marine sediments. Here we study the environmental distribution, abundance and ecogenomics of the gammaproteobacterial Woeseiaceae/JTB255 marine benthic group. A meta-analysis of published work shows that the Woeseiaceae/JTB255 are ubiquitous and consistently rank among the most abundant 16S rRNA gene sequences in diverse marine sediments. They account for up to 22% of bacterial amplicons and 6% of total cell counts in European and Australian coastal sediments. The analysis of a single-cell genome, metagenomic bins and the genome of the next cultured relative Woeseia oceani indicated a broad physiological range, including heterotrophy and facultative autotrophy. All tested (meta)genomes encode a truncated denitrification pathway to nitrous oxide. The broad range of energy-yielding metabolisms possibly explains the ubiquity and high abundance of Woeseiaceae/JTB255 in marine sediments, where they carry out diverse, but yet unknown ecological functions.

  • Microbial nutrient niches in the gut.

    Pereira FC, Berry D
    2017 - Environ. Microbiol., 4: 1366-1378

    Abstract: 

    The composition and function of the mammalian gut microbiota has been the subject of much research in recent years, but the principles underlying the assembly and structure of this complex community remain incompletely understood. Processes that shape the gut microbiota are thought to be mostly niche-driven, with environmental factors such as the composition of available nutrients largely determining whether or not an organism can establish. The concept that the nutrient landscape dictates which organisms can successfully colonize and persist in the gut was first proposed in Rolf Freter's nutrient niche theory. In a situation where nutrients are perfectly mixed and there is balanced microbial growth, Freter postulated that an organism can only survive if it is able to utilize one or a few limiting nutrients more efficiently than its competitors. Recent experimental work indicates, however, that nutrients in the gut vary in space and time. We propose that in such a scenario, Freter's nutrient niche theory must be expanded to account for the co-existence of microorganisms utilizing the same nutrients but in distinct sites or at different times, and that metabolic flexibility and mixed-substrate utilization are common strategies for survival in the face of ever-present nutrient fluctuations.

  • A 12-week intervention with nonivamide, a TRPV1 agonist, prevents a dietary-induced body fat gain and increases peripheral serotonin in moderately overweight subjects.

    Hochkogler CM, Lieder B, Rust P, Berry D, Meier SM, Pignitter M, Riva A, Leitinger A, Bruk A, Wagner S, Hans J, Widder S, Ley JP, Krammer GE, Somoza V
    2017 - Mol Nutr Food Res, 5: 1600731

    Abstract: 

    A bolus administration of 0.15 mg nonivamide has previously been demonstrated to reduce energy intake in moderately overweight men. This 12-week intervention investigated whether a daily consumption of nonivamide in a protein-based product formulation promotes a reduction in body weight in healthy overweight subjects and affects outcome measures associated with mechanisms regulating food intake, e.g. plasma concentrations of (an)orexigenic hormones, energy substrates as well as changes in fecal microbiota.
    Nineteen overweight subjects were randomly assigned to either a control (C) or a nonivamide (NV) group. Changes in the body composition and plasma concentrations of satiating hormones were determined at fasting and 15, 30, 60, 90, and 120 min after a glucose load. Participants were instructed to consume 0.15 mg nonivamide per day in 450 mL of a milk shake additionally to their habitual diet. After treatment, a group difference in body fat mass change (-0.61 ± 0.36% in NV and +1.36 ± 0.38% in C) and an increase in postprandial plasma serotonin were demonstrated. Plasma metabolome and fecal microbiome read outs were not affected.
    A daily intake of 0.15 mg nonivamide helps to support to maintain a healthy body composition.

  • Convergent patterns in the evolution of mealybug symbioses involving different intrabacterial symbionts.

    Szabó G, Schulz F, Toenshoff ER, Volland JM, Finkel OM, Belkin S, Horn M
    2017 - ISME J, 3: 715-726
    Mealybugs (Trabutina mannipara) enveloped in a wax cover feeding on Tamarix twig

    Abstract: 

    Mealybugs (Insecta: Hemiptera: Pseudococcidae) maintain obligatory relationships with bacterial symbionts, which provide essential nutrients to their insect hosts. Most pseudococcinae mealybugs harbor a unique symbiosis setup with enlarged betaproteobacterial symbionts ('Candidatus Tremblaya princeps'), which themselves contain gammaproteobacterial symbionts. Here we investigated the symbiosis of the manna mealybug, Trabutina mannipara, using a metagenomic approach. Phylogenetic analyses revealed that the intrabacterial symbiont of T. mannipara represents a novel lineage within the Gammaproteobacteria, for which we propose the tentative name 'Candidatus Trabutinella endobia'. Combining our results with previous data available for the nested symbiosis of the citrus mealybug Planococcus citri, we show that synthesis of essential amino acids and vitamins and translation-related functions partition between the symbiotic partners in a highly similar manner in the two systems, despite the distinct evolutionary origin of the intrabacterial symbionts. Bacterial genes found in both mealybug genomes and complementing missing functions in both symbioses were likely integrated in ancestral mealybugs before T. mannipara and P. citri diversified. The high level of correspondence between the two mealybug systems and their highly intertwined metabolic pathways are unprecedented. Our work contributes to a better understanding of the only known intracellular symbiosis between two bacteria and suggests that the evolution of this unique symbiosis included the replacement of intrabacterial symbionts in ancestral mealybugs.

  • Happens in the best of subfamilies: establishment and repeated replacements of co-obligate secondary endosymbionts within Lachninae aphids.

    Manzano-Marín A, Szabó G, Simon JC, Horn M, Latorre A
    2017 - Environ. Microbiol., 1: 393-408

    Abstract: 

    Virtually all aphids maintain an obligate mutualistic symbiosis with bacteria from the Buchnera genus, which produce essential nutrients for their aphid hosts. Most aphids from the Lachninae subfamily have been consistently found to house additional endosymbionts, mainly Serratia symbiotica. This apparent dependence on secondary endosymbionts was proposed to have been triggered by the loss of the riboflavin biosynthetic capability by Buchnera in the Lachninae last common ancestor. However, an integral large-scale analysis of secondary endosymbionts in the Lachninae is still missing, hampering the interpretation of the evolutionary and genomic analyses of these endosymbionts. Here, we analysed the endosymbionts of selected representatives from seven different Lachninae genera and nineteen species, spanning four tribes, both by FISH (exploring the symbionts' morphology and tissue tropism) and 16S rRNA gene sequencing. We demonstrate that all analysed aphids possess dual symbiotic systems, and while most harbour S. symbiotica, some have undergone symbiont replacement by other phylogenetically-distinct bacterial taxa. We found that these secondary associates display contrasting cell shapes and tissue tropism, and some appear to be lineage-specific. We propose a scenario for symbiont establishment in the Lachninae, followed by changes in the symbiont's tissue tropism and symbiont replacement events, thereby highlighting the extraordinary versatility of host-symbiont interactions.

  • Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis.

    Ponnudurai R, Kleiner M, Sayavedra L, Petersen JM, Moche M, Otto A, Becher D, Takeuchi T, Satoh N, Dubilier N, Schweder T, Markert S
    2017 - ISME J, 11: 463–477

    Abstract: 

    The hydrothermal vent mussel Bathymodiolus azoricus lives in an intimate symbiosis with two types of chemosynthetic Gammaproteobacteria in its gills: a sulfur oxidizer and a methane oxidizer. Despite numerous investigations over the last decades, the degree of interdependence between the three symbiotic partners, their individual metabolic contributions, as well as the mechanism of carbon transfer from the symbionts to the host are poorly understood. We used a combination of proteomics and genomics to investigate the physiology and metabolism of the individual symbiotic partners. Our study revealed that key metabolic functions are most likely accomplished jointly by B. azoricus and its symbionts: (1) CO2 is pre-concentrated by the host for carbon fixation by the sulfur-oxidizing symbiont, and (2) the host replenishes essential biosynthetic TCA cycle intermediates for the sulfur-oxidizing symbiont. In return (3), the sulfur oxidizer may compensate for the host's putative deficiency in amino acid and cofactor biosynthesis. We also identified numerous 'symbiosis-specific' host proteins by comparing symbiont-containing and symbiont-free host tissues and symbiont fractions. These proteins included a large complement of host digestive enzymes in the gill that are likely involved in symbiont digestion and carbon transfer from the symbionts to the host.The ISME Journal advance online publication, 1 November 2016; doi:10.1038/ismej.2016.124.

  • Stable isotope techniques for the assessment of host and microbiota response during gastrointestinal dysfunction

    Butler RN, Kosek M, Krebs N, Loechl C, Loy A, Owino V, Zimmermann M, and Morrison DJ
    2017 - J Pediatr Gastroenterol Nutr, 64: 8-14

    Abstract: 

    The International Atomic Energy Agency convened a technical meeting on environmental enteric dysfunction (EED) in Vienna (28th – 30th October 2015; https://nucleus.iaea.org/HHW/Nutrition/EED_Technical_Meeting/index.html) to bring together international experts in the fields of EED, nutrition and stable isotope technologies. Advances in stable isotope labelling techniques open up new possibilities to improve our understanding of gastrointestinal dysfunction and the role of the microbiota in host health. In the context of EED, little is known about the role gut dysfunction may play in macro- and micronutrient bioavailability and requirements and what the consequences may be for nutritional status and linear growth. Stable isotope labelling techniques have been used to assess intestinal mucosal injury and barrier function, carbohydrate digestion and fermentation, protein derived amino acid bioavailability and requirements, micronutrient bioavailability and to track microbe-microbe and microbe-host interactions at the single cell level. The non-invasive nature of stable isotope technologies potentially allows for low-hazard, field deployable tests of gut dysfunction that are applicable across all age-groups. The purpose of this review is to assess the state-of-the-art in the use of stable isotope technologies and to provide a perspective on where these technologies can be exploited to further our understanding of gut dysfunction in EED.

  • Pediatric obesity is associated with an altered gut microbiota and discordant shifts in Firmicutes populations

    Riva A, Borgo F, Lassandro C, Verduci E, Morace G, Borghi E, Berry D
    2017 - Environ. Microbiol., 1: 95-105

    Abstract: 

    An altered gut microbiota has been linked to obesity in adulthood, although little is known about childhood obesity. The aim of this study was to characterize the composition of the gut microbiota in obese (n = 42) and normal-weight (n = 36) children aged 6 to 16. Using 16S rRNA gene-targeted sequencing, we evaluated taxa with differential abundance according to age- and sex-normalized body mass index (BMI z-score). Obesity was associated with an altered gut microbiota characterized by elevated levels of Firmicutes and depleted levels of Bacteroidetes. Correlation network analysis revealed that the gut microbiota of obese children also had increased correlation density and clustering of operational taxonomic units (OTUs). Members of the Bacteroidetes were generally better predictors of BMI z-score and obesity than Firmicutes, which was likely due to discordant responses of Firmicutes OTUs. In accordance with these observations, the main metabolites produced by gut bacteria, short chain fatty acids (SCFAs), were higher in obese children, suggesting elevated substrate utilisation. Multiple taxa were correlated with SCFA levels, reinforcing the tight link between the microbiota, SCFAs and obesity. Our results suggest that gut microbiota dysbiosis and elevated fermentation activity may be involved in the etiology of childhood obesity.

Book chapters and other publications

4 Publications found
  • Terriglobus

    Eichorst SA, Trojan D, Woebken D
    2017 - in Bergey's Manual of Systematics of Archaea and Bacteria.. (Whitman WB, Rainey F, Kämpfer P, Trujillo M, Chun J, DeVos P, Hedlund B, Dedysh S)

    Abstract: 

    Terriglobus is a genus in the phylum Acidobacteria in the family Acidobacteriaceae, order Acidobacteriales, class Acidobacteriia, subdivision 1. It currently comprises five species - Terriglobus roseus, Terriglobus saanensis, Terriglobus tenax, Terriglobus aquaticus, and Terriglobus albidus. Members of the genus are widely distributed in soils including rhizosphere soils and the phyllosphere, but is also found in freshwater and in association with insects. This genus encompasses bacteria that are chemo-organotrophs and have obligatory aerobic metabolism with an optimal growth in mildly acidic (pH ~5 to 6) and mesophilic (ca. 25 to 30°C) conditions. Colonies of Terriglobus are typically circular in form with a convex elevation and can be with or without pink pigmentation. These bacteria can use a range of different carbon sources, and nitrogen is attained by exogenous amino acids or ammonium chloride. Cells are non-motile, Gram-stain-negative with a length and width ranging from 0.8 to 2.5 µm and 0.4 to 0.9 µm, respectively. Some strains produce extracellular material, which can be visualized by microscopy or in liquid culture, generating a floc/clumping phenotype. The dominant fatty acids are iso-C15:0 and C16:1 ω7c/ C16:1 ω6c. The DNA G+C content (mol%) ranges from 57.3 to 63.2%.

  • Hidden potential: Diet-driven changes in redox level shape the rumen microbiome

    2017 - Environmental Microbiology, 1: 19-20
  • The unexpected versatility of the cellulosome

    2017 - Environmental Microbiology, 1: 13-14
  • Principles of Systems Biology, No. 13 - A pathogen-resistant designer microbiota

    Stecher B, Clavel T, Loy A, Berry D
    2017 - Cell Systems, 4: 3-6