Harrison JP 1767 Horn M
Harrison JP 1767 Daims H
Harrison JP 1767 Berry D
Harrison JP 1767 Loy A
Harrison JP 1767 Wagner M
Harrison JP 1767 Eichorst SA
Harrison JP 1767 Mussmann M
Harrison JP 1767 Wasmund K
Harrison JP 1767 Herbold CW
Harrison JP 1767 Sedlacek CJ
Angel R 325 Horn M
Angel R 325 Daims H
Angel R 325 Berry D
Angel R 325 Loy A
Angel R 325 Wagner M
Angel R 325 Eichorst SA
Angel R 325 Mussmann M
Angel R 325 Wasmund K
Angel R 325 Herbold CW
Angel R 325 Sedlacek CJ
Cockell CS 1781 Horn M
Cockell CS 1781 Daims H
Cockell CS 1781 Berry D
Cockell CS 1781 Loy A
Cockell CS 1781 Wagner M
Cockell CS 1781 Eichorst SA
Cockell CS 1781 Mussmann M
Cockell CS 1781 Wasmund K
Cockell CS 1781 Herbold CW
Cockell CS 1781 Sedlacek CJ
Pjevac P 1297 Horn M
Pjevac P 1297 Daims H
Pjevac P 1297 Berry D
Pjevac P 1297 Loy A
Pjevac P 1297 Wagner M
Pjevac P 1297 Eichorst SA
Pjevac P 1297 Mussmann M
Pjevac P 1297 Wasmund K
Pjevac P 1297 Herbold CW
Pjevac P 1297 Sedlacek CJ
Schauberger C 1897 Horn M
Schauberger C 1897 Daims H
Schauberger C 1897 Berry D
Schauberger C 1897 Loy A
Schauberger C 1897 Wagner M
Schauberger C 1897 Eichorst SA
Schauberger C 1897 Mussmann M
Schauberger C 1897 Wasmund K
Schauberger C 1897 Herbold CW
Schauberger C 1897 Sedlacek CJ
Poghosyan L 1898 Horn M
Poghosyan L 1898 Daims H
Poghosyan L 1898 Berry D
Poghosyan L 1898 Loy A
Poghosyan L 1898 Wagner M
Poghosyan L 1898 Eichorst SA
Poghosyan L 1898 Mussmann M
Poghosyan L 1898 Wasmund K
Poghosyan L 1898 Herbold CW
Poghosyan L 1898 Sedlacek CJ
Herbold CW 321 Horn M
Herbold CW 321 Daims H
Herbold CW 321 Berry D
Herbold CW 321 Loy A
Herbold CW 321 Wagner M
Herbold CW 321 Eichorst SA
Herbold CW 321 Mussmann M
Herbold CW 321 Wasmund K
Herbold CW 321 Herbold CW
Herbold CW 321 Sedlacek CJ
van Kessel MAHJ 1899 Horn M
van Kessel MAHJ 1899 Daims H
van Kessel MAHJ 1899 Berry D
van Kessel MAHJ 1899 Loy A
van Kessel MAHJ 1899 Wagner M
van Kessel MAHJ 1899 Eichorst SA
van Kessel MAHJ 1899 Mussmann M
van Kessel MAHJ 1899 Wasmund K
van Kessel MAHJ 1899 Herbold CW
van Kessel MAHJ 1899 Sedlacek CJ
Daebeler A 1900 Horn M
Daebeler A 1900 Daims H
Daebeler A 1900 Berry D
Daebeler A 1900 Loy A
Daebeler A 1900 Wagner M
Daebeler A 1900 Eichorst SA
Daebeler A 1900 Mussmann M
Daebeler A 1900 Wasmund K
Daebeler A 1900 Herbold CW
Daebeler A 1900 Sedlacek CJ
Steinberger M 1837 Horn M
Steinberger M 1837 Daims H
Steinberger M 1837 Berry D
Steinberger M 1837 Loy A
Steinberger M 1837 Wagner M
Steinberger M 1837 Eichorst SA
Steinberger M 1837 Mussmann M
Steinberger M 1837 Wasmund K
Steinberger M 1837 Herbold CW
Steinberger M 1837 Sedlacek CJ
Jetten MSM 487 Horn M
Jetten MSM 487 Daims H
Jetten MSM 487 Berry D
Jetten MSM 487 Loy A
Jetten MSM 487 Wagner M
Jetten MSM 487 Eichorst SA
Jetten MSM 487 Mussmann M
Jetten MSM 487 Wasmund K
Jetten MSM 487 Herbold CW
Jetten MSM 487 Sedlacek CJ
Luecker S 1901 Horn M
Luecker S 1901 Daims H
Luecker S 1901 Berry D
Luecker S 1901 Loy A
Luecker S 1901 Wagner M
Luecker S 1901 Eichorst SA
Luecker S 1901 Mussmann M
Luecker S 1901 Wasmund K
Luecker S 1901 Herbold CW
Luecker S 1901 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Böck D 1905 Horn M
Böck D 1905 Daims H
Böck D 1905 Berry D
Böck D 1905 Loy A
Böck D 1905 Wagner M
Böck D 1905 Eichorst SA
Böck D 1905 Mussmann M
Böck D 1905 Wasmund K
Böck D 1905 Herbold CW
Böck D 1905 Sedlacek CJ
Medeiros JM 1906 Horn M
Medeiros JM 1906 Daims H
Medeiros JM 1906 Berry D
Medeiros JM 1906 Loy A
Medeiros JM 1906 Wagner M
Medeiros JM 1906 Eichorst SA
Medeiros JM 1906 Mussmann M
Medeiros JM 1906 Wasmund K
Medeiros JM 1906 Herbold CW
Medeiros JM 1906 Sedlacek CJ
Tsao HF 1704 Horn M
Tsao HF 1704 Daims H
Tsao HF 1704 Berry D
Tsao HF 1704 Loy A
Tsao HF 1704 Wagner M
Tsao HF 1704 Eichorst SA
Tsao HF 1704 Mussmann M
Tsao HF 1704 Wasmund K
Tsao HF 1704 Herbold CW
Tsao HF 1704 Sedlacek CJ
Penz T 779 Horn M
Penz T 779 Daims H
Penz T 779 Berry D
Penz T 779 Loy A
Penz T 779 Wagner M
Penz T 779 Eichorst SA
Penz T 779 Mussmann M
Penz T 779 Wasmund K
Penz T 779 Herbold CW
Penz T 779 Sedlacek CJ
Weiss GL 1907 Horn M
Weiss GL 1907 Daims H
Weiss GL 1907 Berry D
Weiss GL 1907 Loy A
Weiss GL 1907 Wagner M
Weiss GL 1907 Eichorst SA
Weiss GL 1907 Mussmann M
Weiss GL 1907 Wasmund K
Weiss GL 1907 Herbold CW
Weiss GL 1907 Sedlacek CJ
Aistleitner K 258 Horn M
Aistleitner K 258 Daims H
Aistleitner K 258 Berry D
Aistleitner K 258 Loy A
Aistleitner K 258 Wagner M
Aistleitner K 258 Eichorst SA
Aistleitner K 258 Mussmann M
Aistleitner K 258 Wasmund K
Aistleitner K 258 Herbold CW
Aistleitner K 258 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Pilhofer M 540 Horn M
Pilhofer M 540 Daims H
Pilhofer M 540 Berry D
Pilhofer M 540 Loy A
Pilhofer M 540 Wagner M
Pilhofer M 540 Eichorst SA
Pilhofer M 540 Mussmann M
Pilhofer M 540 Wasmund K
Pilhofer M 540 Herbold CW
Pilhofer M 540 Sedlacek CJ
König L 542 Horn M
König L 542 Daims H
König L 542 Berry D
König L 542 Loy A
König L 542 Wagner M
König L 542 Eichorst SA
König L 542 Mussmann M
König L 542 Wasmund K
König L 542 Herbold CW
König L 542 Sedlacek CJ
Siegl A 871 Horn M
Siegl A 871 Daims H
Siegl A 871 Berry D
Siegl A 871 Loy A
Siegl A 871 Wagner M
Siegl A 871 Eichorst SA
Siegl A 871 Mussmann M
Siegl A 871 Wasmund K
Siegl A 871 Herbold CW
Siegl A 871 Sedlacek CJ
Penz T 779 Horn M
Penz T 779 Daims H
Penz T 779 Berry D
Penz T 779 Loy A
Penz T 779 Wagner M
Penz T 779 Eichorst SA
Penz T 779 Mussmann M
Penz T 779 Wasmund K
Penz T 779 Herbold CW
Penz T 779 Sedlacek CJ
Haider S 958 Horn M
Haider S 958 Daims H
Haider S 958 Berry D
Haider S 958 Loy A
Haider S 958 Wagner M
Haider S 958 Eichorst SA
Haider S 958 Mussmann M
Haider S 958 Wasmund K
Haider S 958 Herbold CW
Haider S 958 Sedlacek CJ
Wentrup C 1125 Horn M
Wentrup C 1125 Daims H
Wentrup C 1125 Berry D
Wentrup C 1125 Loy A
Wentrup C 1125 Wagner M
Wentrup C 1125 Eichorst SA
Wentrup C 1125 Mussmann M
Wentrup C 1125 Wasmund K
Wentrup C 1125 Herbold CW
Wentrup C 1125 Sedlacek CJ
Polzin J 1889 Horn M
Polzin J 1889 Daims H
Polzin J 1889 Berry D
Polzin J 1889 Loy A
Polzin J 1889 Wagner M
Polzin J 1889 Eichorst SA
Polzin J 1889 Mussmann M
Polzin J 1889 Wasmund K
Polzin J 1889 Herbold CW
Polzin J 1889 Sedlacek CJ
Mann E 1890 Horn M
Mann E 1890 Daims H
Mann E 1890 Berry D
Mann E 1890 Loy A
Mann E 1890 Wagner M
Mann E 1890 Eichorst SA
Mann E 1890 Mussmann M
Mann E 1890 Wasmund K
Mann E 1890 Herbold CW
Mann E 1890 Sedlacek CJ
Schmitz-Esser S 593 Horn M
Schmitz-Esser S 593 Daims H
Schmitz-Esser S 593 Berry D
Schmitz-Esser S 593 Loy A
Schmitz-Esser S 593 Wagner M
Schmitz-Esser S 593 Eichorst SA
Schmitz-Esser S 593 Mussmann M
Schmitz-Esser S 593 Wasmund K
Schmitz-Esser S 593 Herbold CW
Schmitz-Esser S 593 Sedlacek CJ
Domman D 299 Horn M
Domman D 299 Daims H
Domman D 299 Berry D
Domman D 299 Loy A
Domman D 299 Wagner M
Domman D 299 Eichorst SA
Domman D 299 Mussmann M
Domman D 299 Wasmund K
Domman D 299 Herbold CW
Domman D 299 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Singer E 1858 Horn M
Singer E 1858 Daims H
Singer E 1858 Berry D
Singer E 1858 Loy A
Singer E 1858 Wagner M
Singer E 1858 Eichorst SA
Singer E 1858 Mussmann M
Singer E 1858 Wasmund K
Singer E 1858 Herbold CW
Singer E 1858 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Woyke T 616 Horn M
Woyke T 616 Daims H
Woyke T 616 Berry D
Woyke T 616 Loy A
Woyke T 616 Wagner M
Woyke T 616 Eichorst SA
Woyke T 616 Mussmann M
Woyke T 616 Wasmund K
Woyke T 616 Herbold CW
Woyke T 616 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Gutierrez T 642 Horn M
Gutierrez T 642 Daims H
Gutierrez T 642 Berry D
Gutierrez T 642 Loy A
Gutierrez T 642 Wagner M
Gutierrez T 642 Eichorst SA
Gutierrez T 642 Mussmann M
Gutierrez T 642 Wasmund K
Gutierrez T 642 Herbold CW
Gutierrez T 642 Sedlacek CJ
Harrison JP 1767 Horn M
Harrison JP 1767 Daims H
Harrison JP 1767 Berry D
Harrison JP 1767 Loy A
Harrison JP 1767 Wagner M
Harrison JP 1767 Eichorst SA
Harrison JP 1767 Mussmann M
Harrison JP 1767 Wasmund K
Harrison JP 1767 Herbold CW
Harrison JP 1767 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Schulz F 296 Horn M
Schulz F 296 Daims H
Schulz F 296 Berry D
Schulz F 296 Loy A
Schulz F 296 Wagner M
Schulz F 296 Eichorst SA
Schulz F 296 Mussmann M
Schulz F 296 Wasmund K
Schulz F 296 Herbold CW
Schulz F 296 Sedlacek CJ
Yutin N 1873 Horn M
Yutin N 1873 Daims H
Yutin N 1873 Berry D
Yutin N 1873 Loy A
Yutin N 1873 Wagner M
Yutin N 1873 Eichorst SA
Yutin N 1873 Mussmann M
Yutin N 1873 Wasmund K
Yutin N 1873 Herbold CW
Yutin N 1873 Sedlacek CJ
Ivanova NN 1870 Horn M
Ivanova NN 1870 Daims H
Ivanova NN 1870 Berry D
Ivanova NN 1870 Loy A
Ivanova NN 1870 Wagner M
Ivanova NN 1870 Eichorst SA
Ivanova NN 1870 Mussmann M
Ivanova NN 1870 Wasmund K
Ivanova NN 1870 Herbold CW
Ivanova NN 1870 Sedlacek CJ
Ortega DR 1871 Horn M
Ortega DR 1871 Daims H
Ortega DR 1871 Berry D
Ortega DR 1871 Loy A
Ortega DR 1871 Wagner M
Ortega DR 1871 Eichorst SA
Ortega DR 1871 Mussmann M
Ortega DR 1871 Wasmund K
Ortega DR 1871 Herbold CW
Ortega DR 1871 Sedlacek CJ
Lee TK 277 Horn M
Lee TK 277 Daims H
Lee TK 277 Berry D
Lee TK 277 Loy A
Lee TK 277 Wagner M
Lee TK 277 Eichorst SA
Lee TK 277 Mussmann M
Lee TK 277 Wasmund K
Lee TK 277 Herbold CW
Lee TK 277 Sedlacek CJ
Vierheilig J 1298 Horn M
Vierheilig J 1298 Daims H
Vierheilig J 1298 Berry D
Vierheilig J 1298 Loy A
Vierheilig J 1298 Wagner M
Vierheilig J 1298 Eichorst SA
Vierheilig J 1298 Mussmann M
Vierheilig J 1298 Wasmund K
Vierheilig J 1298 Herbold CW
Vierheilig J 1298 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Jensen GJ 543 Horn M
Jensen GJ 543 Daims H
Jensen GJ 543 Berry D
Jensen GJ 543 Loy A
Jensen GJ 543 Wagner M
Jensen GJ 543 Eichorst SA
Jensen GJ 543 Mussmann M
Jensen GJ 543 Wasmund K
Jensen GJ 543 Herbold CW
Jensen GJ 543 Sedlacek CJ
Kyrpides NC 613 Horn M
Kyrpides NC 613 Daims H
Kyrpides NC 613 Berry D
Kyrpides NC 613 Loy A
Kyrpides NC 613 Wagner M
Kyrpides NC 613 Eichorst SA
Kyrpides NC 613 Mussmann M
Kyrpides NC 613 Wasmund K
Kyrpides NC 613 Herbold CW
Kyrpides NC 613 Sedlacek CJ
Koonin EV 1872 Horn M
Koonin EV 1872 Daims H
Koonin EV 1872 Berry D
Koonin EV 1872 Loy A
Koonin EV 1872 Wagner M
Koonin EV 1872 Eichorst SA
Koonin EV 1872 Mussmann M
Koonin EV 1872 Wasmund K
Koonin EV 1872 Herbold CW
Koonin EV 1872 Sedlacek CJ
Woyke T 616 Horn M
Woyke T 616 Daims H
Woyke T 616 Berry D
Woyke T 616 Loy A
Woyke T 616 Wagner M
Woyke T 616 Eichorst SA
Woyke T 616 Mussmann M
Woyke T 616 Wasmund K
Woyke T 616 Herbold CW
Woyke T 616 Sedlacek CJ
Zojer M 1860 Horn M
Zojer M 1860 Daims H
Zojer M 1860 Berry D
Zojer M 1860 Loy A
Zojer M 1860 Wagner M
Zojer M 1860 Eichorst SA
Zojer M 1860 Mussmann M
Zojer M 1860 Wasmund K
Zojer M 1860 Herbold CW
Zojer M 1860 Sedlacek CJ
Schuster LN 1861 Horn M
Schuster LN 1861 Daims H
Schuster LN 1861 Berry D
Schuster LN 1861 Loy A
Schuster LN 1861 Wagner M
Schuster LN 1861 Eichorst SA
Schuster LN 1861 Mussmann M
Schuster LN 1861 Wasmund K
Schuster LN 1861 Herbold CW
Schuster LN 1861 Sedlacek CJ
Schulz F 296 Horn M
Schulz F 296 Daims H
Schulz F 296 Berry D
Schulz F 296 Loy A
Schulz F 296 Wagner M
Schulz F 296 Eichorst SA
Schulz F 296 Mussmann M
Schulz F 296 Wasmund K
Schulz F 296 Herbold CW
Schulz F 296 Sedlacek CJ
Pfundner A 1862 Horn M
Pfundner A 1862 Daims H
Pfundner A 1862 Berry D
Pfundner A 1862 Loy A
Pfundner A 1862 Wagner M
Pfundner A 1862 Eichorst SA
Pfundner A 1862 Mussmann M
Pfundner A 1862 Wasmund K
Pfundner A 1862 Herbold CW
Pfundner A 1862 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Rattei T 307 Horn M
Rattei T 307 Daims H
Rattei T 307 Berry D
Rattei T 307 Loy A
Rattei T 307 Wagner M
Rattei T 307 Eichorst SA
Rattei T 307 Mussmann M
Rattei T 307 Wasmund K
Rattei T 307 Herbold CW
Rattei T 307 Sedlacek CJ
Butler RN 1483 Horn M
Butler RN 1483 Daims H
Butler RN 1483 Berry D
Butler RN 1483 Loy A
Butler RN 1483 Wagner M
Butler RN 1483 Eichorst SA
Butler RN 1483 Mussmann M
Butler RN 1483 Wasmund K
Butler RN 1483 Herbold CW
Butler RN 1483 Sedlacek CJ
Kosek M 1484 Horn M
Kosek M 1484 Daims H
Kosek M 1484 Berry D
Kosek M 1484 Loy A
Kosek M 1484 Wagner M
Kosek M 1484 Eichorst SA
Kosek M 1484 Mussmann M
Kosek M 1484 Wasmund K
Kosek M 1484 Herbold CW
Kosek M 1484 Sedlacek CJ
Krebs N 1485 Horn M
Krebs N 1485 Daims H
Krebs N 1485 Berry D
Krebs N 1485 Loy A
Krebs N 1485 Wagner M
Krebs N 1485 Eichorst SA
Krebs N 1485 Mussmann M
Krebs N 1485 Wasmund K
Krebs N 1485 Herbold CW
Krebs N 1485 Sedlacek CJ
Loechl C 1486 Horn M
Loechl C 1486 Daims H
Loechl C 1486 Berry D
Loechl C 1486 Loy A
Loechl C 1486 Wagner M
Loechl C 1486 Eichorst SA
Loechl C 1486 Mussmann M
Loechl C 1486 Wasmund K
Loechl C 1486 Herbold CW
Loechl C 1486 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Owino V 1487 Horn M
Owino V 1487 Daims H
Owino V 1487 Berry D
Owino V 1487 Loy A
Owino V 1487 Wagner M
Owino V 1487 Eichorst SA
Owino V 1487 Mussmann M
Owino V 1487 Wasmund K
Owino V 1487 Herbold CW
Owino V 1487 Sedlacek CJ
Zimmermann M 1488 Horn M
Zimmermann M 1488 Daims H
Zimmermann M 1488 Berry D
Zimmermann M 1488 Loy A
Zimmermann M 1488 Wagner M
Zimmermann M 1488 Eichorst SA
Zimmermann M 1488 Mussmann M
Zimmermann M 1488 Wasmund K
Zimmermann M 1488 Herbold CW
Zimmermann M 1488 Sedlacek CJ
and Morrison DJ 1489 Horn M
and Morrison DJ 1489 Daims H
and Morrison DJ 1489 Berry D
and Morrison DJ 1489 Loy A
and Morrison DJ 1489 Wagner M
and Morrison DJ 1489 Eichorst SA
and Morrison DJ 1489 Mussmann M
and Morrison DJ 1489 Wasmund K
and Morrison DJ 1489 Herbold CW
and Morrison DJ 1489 Sedlacek CJ
Publications | Microbial Ecology, University of Vienna

Publications

Publications in peer reviewed journals

10 Publications found
  • Astrobiology as a framework for investigating antibiotic susceptibility: a study of Halomonas hydrothermalis

    Harrison JP, Angel R, Cockell CS
    2017 - J R Soc Interface, 126: online only

    Abstract: 

    Physical and chemical boundaries for microbial multiplication on Earth are strongly influenced by interactions between environmental extremes. However, little is known about how interactions between multiple stress parameters affect the sensitivity of microorganisms to antibiotics. Here, we assessed how 12 distinct permutations of salinity, availability of an essential nutrient (iron) and atmospheric composition (aerobic or microaerobic) affect the susceptibility of a polyextremotolerant bacterium, Halomonas hydrothermalis, to ampicillin, kanamycin and ofloxacin. While salinity had a significant impact on sensitivity to all three antibiotics (as shown by turbidimetric analyses), the nature of this impact was modified by iron availability and the ambient gas composition, with differing effects observed for each compound. These two parameters were found to be of particular importance when considered in combination and, in the case of ampicillin, had a stronger combined influence on antibiotic tolerance than salinity. Our data show how investigating microbial responses to multiple extremes, which are more representative of natural habitats than single extremes, can improve our understanding of the effects of antimicrobial compounds and suggest how studies of habitability, motivated by the desire to map the limits of life, can be used to systematically assess the effectiveness of antibiotics.

  • AmoA-targeted polymerase chain reaction primers for the specific detection and quantification of comammox Nitrospira in the environment

    Pjevac P, Schauberger C, Poghosyan L, Herbold CW, van Kessel MAHJ, Daebeler A, Steinberger M, Jetten MSM, Luecker S, Wagner M, Daims H
    2017 - Front Microbiol, 8:1508
    Comammox PCR primers

    Abstract: 

    Nitrification, the oxidation of ammonia via nitrite to nitrate, has always been considered to be catalyzed by the concerted activity of ammonia- and nitrite-oxidizing microorganisms. Only recently, complete ammonia oxidizers (‘comammox’), which oxidize ammonia to nitrate on their own, were identified in the bacterial genus Nitrospira, previously assumed to contain only canonical nitrite oxidizers. Nitrospira are widespread in nature, but for assessments of the distribution and functional importance of comammox Nitrospira in ecosystems, cultivation-independent tools to distinguish comammox from strictly nitrite oxidizing Nitrospira are required. Here we developed new PCR primer sets that specifically target the amoA genes coding for subunit A of the distinct ammonia monooxygenase of comammox Nitrospira. While existing primers capture only a fraction of the known comammox amoA diversity, the new primer sets cover as much as 95% of the comammox amoA clade A and 92% of the clade B sequences in a reference database containing 326 comammox amoA genes with sequence information at the primer binding sites. Application of the primers to 13 samples from engineered systems (a groundwater well, drinking water treatment and wastewater treatment plants) and other habitats (rice paddy and forest soils, rice rhizosphere, brackish lake sediment and freshwater biofilm) detected comammox Nitrospira in all samples and revealed a considerable diversity of comammox in most habitats. Excellent primer specificity for comammox amoA was achieved by avoiding the use of highly degenerate primer preparations and by using equimolar mixtures of oligonucleotides that match existing comammox amoA genes. Quantitative PCR with these equimolar primer mixtures was highly sensitive and specific, and enabled the efficient quantification of clade A and clade B comammox amoA gene copy numbers in environmental samples. The measured relative abundances of comammox Nitrospira, compared to canonical ammonia oxidizers, were highly variable across environments. The new comammox amoA-targeted primers enable more encompassing future studies of nitrifying microorganisms in diverse habitats. For example, they may be used to monitor the population dynamics of uncultured comammox organisms under changing environmental conditions and in response to altered treatments in engineered and agricultural ecosystems.

  • In situ architecture, function, and evolution of a contractile injection system.

    Böck D, Medeiros JM, Tsao HF, Penz T, Weiss GL, Aistleitner K, Horn M, Pilhofer M
    2017 - Science, 6352: 713-717

    Abstract: 

    Contractile injection systems mediate bacterial cell-cell interactions by a bacteriophage tail-like structure. In contrast to extracellular systems, the type 6 secretion system (T6SS) is defined by intracellular localization and attachment to the cytoplasmic membrane. Here we used cryo-focused ion beam milling, electron cryotomography, and functional assays to study a T6SS in Amoebophilus asiaticus The in situ architecture revealed three modules, including a contractile sheath-tube, a baseplate, and an anchor. All modules showed conformational changes upon firing. Lateral baseplate interactions coordinated T6SSs in hexagonal arrays. The system mediated interactions with host membranes and may participate in phagosome escape. Evolutionary sequence analyses predicted that T6SSs are more widespread than previously thought. Our insights form the basis for understanding T6SS key concepts and exploring T6SS diversity.

  • Biphasic Metabolism and Host Interaction of a Chlamydial Symbiont.

    König L, Siegl A, Penz T, Haider S, Wentrup C, Polzin J, Mann E, Schmitz-Esser S, Domman D, Horn M
    2017 - mSystems, 2: e00202-16

    Abstract: 

    Chlamydiae are obligate intracellular bacteria comprising well-known human pathogens and ubiquitous symbionts of protists, which are characterized by a unique developmental cycle. Here we comprehensively analyzed gene expression dynamics of Protochlamydia amoebophila during infection of its Acanthamoeba host by RNA sequencing. This revealed a highly dynamic transcriptional landscape, where major transcriptional shifts are conserved among chlamydial symbionts and pathogens. Our data served to propose a time-resolved model for type III protein secretion during the developmental cycle, and we provide evidence for a biphasic metabolism of P. amoebophila during infection, which involves energy parasitism and amino acids as the carbon source during initial stages and a postreplicative switch to endogenous glucose-based ATP production. This fits well with major transcriptional changes in the amoeba host, where upregulation of complex sugar breakdown precedes the P. amoebophila metabolic switch. The biphasic chlamydial metabolism represents a unique adaptation to exploit eukaryotic host cells, which likely contributed to the evolutionary success of this group of microbes. IMPORTANCE Chlamydiae are known as major bacterial pathogens of humans, causing the ancient disease trachoma, but they are also frequently found in the environment where they infect ubiquitous protists such as amoebae. All known chlamydiae require a eukaryotic host cell to thrive. Using the environmental chlamydia Protochlamydia amoebophila within its natural host, Acanthamoeba castellanii, we investigated gene expression dynamics in vivo and throughout the complete chlamydial developmental cycle for the first time. This allowed us to infer how a major virulence mechanism, the type III secretion system, is regulated and employed, and we show that the physiology of chlamydiae undergoes a complete shift regarding carbon metabolism and energy generation. This study provides comprehensive insights into the infection strategy of chlamydiae and reveals a unique adaptation to life within a eukaryotic host cell.

  • Capturing the genetic makeup of the active microbiome in situ.

    Singer E, Wagner M, Woyke T
    2017 - ISME J, 9: 1949-1963

    Abstract: 

    More than any other technology, nucleic acid sequencing has enabled microbial ecology studies to be complemented with the data volumes necessary to capture the extent of microbial diversity and dynamics in a wide range of environments. In order to truly understand and predict environmental processes, however, the distinction between active, inactive and dead microbial cells is critical. Also, experimental designs need to be sensitive toward varying population complexity and activity, and temporal as well as spatial scales of process rates. There are a number of approaches, including single-cell techniques, which were designed to study in situ microbial activity and that have been successively coupled to nucleic acid sequencing. The exciting new discoveries regarding in situ microbial activity provide evidence that future microbial ecology studies will indispensably rely on techniques that specifically capture members of the microbiome active in the environment. Herein, we review those currently used activity-based approaches that can be directly linked to shotgun nucleic acid sequencing, evaluate their relevance to ecology studies, and discuss future directions.

  • Evaluating the Detection of Hydrocarbon-Degrading Bacteria in 16S rRNA Gene Sequencing Surveys.

    Berry D, Gutierrez T
    2017 - Front Microbiol, 8: 2460

    Abstract: 

    Hydrocarbonoclastic bacteria (HCB) play a key role in the biodegradation of oil hydrocarbons in marine and other environments. A small number of taxa have been identified as obligate HCB, notably the Gammaproteobacterial genera Alcanivorax, Cycloclasticus, Marinobacter, Neptumonas, Oleiphilus, Oleispira, and Thalassolituus, as well as the Alphaproteobacterial genus Thalassospira. Detection of HCB in amplicon-based sequencing surveys relies on high coverage by PCR primers and accurate taxonomic classification. In this study, we performed a phylogenetic analysis to identify 16S rRNA gene sequence regions that represent the breadth of sequence diversity within these taxa. Using validated sequences, we evaluated 449 universal 16S rRNA gene-targeted bacterial PCR primer pairs for their coverage of these taxa. The results of this analysis provide a practical framework for selection of suitable primer sets for optimal detection of HCB in sequencing surveys.

  • Vibrational Spectroscopy for Imaging Single Microbial Cells in Complex Biological Samples.

    Harrison JP, Berry D
    2017 - Front Microbiol, 8: 675

    Abstract: 

    Vibrational spectroscopy is increasingly used for the rapid and non-destructive imaging of environmental and medical samples. Both Raman and Fourier-transform infrared (FT-IR) imaging have been applied to obtain detailed information on the chemical composition of biological materials, ranging from single microbial cells to tissues. Due to its compatibility with methods such as stable isotope labeling for the monitoring of cellular activities, vibrational spectroscopy also holds considerable power as a tool in microbial ecology. Chemical imaging of undisturbed biological systems (such as live cells in their native habitats) presents unique challenges due to the physical and chemical complexity of the samples, potential for spectral interference, and frequent need for real-time measurements. This Mini Review provides a critical synthesis of recent applications of Raman and FT-IR spectroscopy for characterizing complex biological samples, with a focus on developments in single-cell imaging. We also discuss how new spectroscopic methods could be used to overcome current limitations of single-cell analyses. Given the inherent complementarity of Raman and FT-IR spectroscopic methods, we discuss how combining these approaches could enable us to obtain new insights into biological activities either in situ or under conditions that simulate selected properties of the natural environment.

  • Giant viruses with an expanded complement of translation system components.

    Schulz F, Yutin N, Ivanova NN, Ortega DR, Lee TK, Vierheilig J, Daims H, Horn M, Wagner M, Jensen GJ, Kyrpides NC, Koonin EV, Woyke T
    2017 - Science, 6333: 82-85
    Klosneuvirus

    Abstract: 

    The discovery of giant viruses blurred the sharp division between viruses and cellular life. Giant virus genomes encode proteins considered as signatures of cellular organisms, particularly translation system components, prompting hypotheses that these viruses derived from a fourth domain of cellular life. Here we report the discovery of a group of giant viruses (Klosneuviruses) in metagenomic data. Compared with other giant viruses, the Klosneuviruses encode an expanded translation machinery, including aminoacyl transfer RNA synthetases with specificities for all 20 amino acids. Notwithstanding the prevalence of translation system components, comprehensive phylogenomic analysis of these genes indicates that Klosneuviruses did not evolve from a cellular ancestor but rather are derived from a much smaller virus through extensive gain of host genes.

  • Variant profiling of evolving prokaryotic populations.

    Zojer M, Schuster LN, Schulz F, Pfundner A, Horn M, Rattei T
    2017 - PeerJ, e2997

    Abstract: 

    Genomic heterogeneity of bacterial species is observed and studied in experimental evolution experiments and clinical diagnostics, and occurs as micro-diversity of natural habitats. The challenge for genome research is to accurately capture this heterogeneity with the currently used short sequencing reads. Recent advances in NGS technologies improved the speed and coverage and thus allowed for deep sequencing of bacterial populations. This facilitates the quantitative assessment of genomic heterogeneity, including low frequency alleles or haplotypes. However, false positive variant predictions due to sequencing errors and mapping artifacts of short reads need to be prevented. We therefore created VarCap, a workflow for the reliable prediction of different types of variants even at low frequencies. In order to predict SNPs, InDels and structural variations, we evaluated the sensitivity and accuracy of different software tools using synthetic read data. The results suggested that the best sensitivity could be reached by a union of different tools, however at the price of increased false positives. We identified possible reasons for false predictions and used this knowledge to improve the accuracy by post-filtering the predicted variants according to properties such as frequency, coverage, genomic environment/localization and co-localization with other variants. We observed that best precision was achieved by using an intersection of at least two tools per variant. This resulted in the reliable prediction of variants above a minimum relative abundance of 2%. VarCap is designed for being routinely used within experimental evolution experiments or for clinical diagnostics. The detected variants are reported as frequencies within a VCF file and as a graphical overview of the distribution of the different variant/allele/haplotype frequencies. The source code of VarCap is available at https://github.com/ma2o/VarCap. In order to provide this workflow to a broad community, we implemeted VarCap on a Galaxy webserver, which is accessible at http://galaxy.csb.univie.ac.at.

  • Stable isotope techniques for the assessment of host and microbiota response during gastrointestinal dysfunction

    Butler RN, Kosek M, Krebs N, Loechl C, Loy A, Owino V, Zimmermann M, and Morrison DJ
    2017 - J Pediatr Gastroenterol Nutr, 64: 8-14

    Abstract: 

    The International Atomic Energy Agency convened a technical meeting on environmental enteric dysfunction (EED) in Vienna (28th – 30th October 2015; https://nucleus.iaea.org/HHW/Nutrition/EED_Technical_Meeting/index.html) to bring together international experts in the fields of EED, nutrition and stable isotope technologies. Advances in stable isotope labelling techniques open up new possibilities to improve our understanding of gastrointestinal dysfunction and the role of the microbiota in host health. In the context of EED, little is known about the role gut dysfunction may play in macro- and micronutrient bioavailability and requirements and what the consequences may be for nutritional status and linear growth. Stable isotope labelling techniques have been used to assess intestinal mucosal injury and barrier function, carbohydrate digestion and fermentation, protein derived amino acid bioavailability and requirements, micronutrient bioavailability and to track microbe-microbe and microbe-host interactions at the single cell level. The non-invasive nature of stable isotope technologies potentially allows for low-hazard, field deployable tests of gut dysfunction that are applicable across all age-groups. The purpose of this review is to assess the state-of-the-art in the use of stable isotope technologies and to provide a perspective on where these technologies can be exploited to further our understanding of gut dysfunction in EED.

Book chapters and other publications

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