Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Stecher B 801 Horn M
Stecher B 801 Daims H
Stecher B 801 Berry D
Stecher B 801 Loy A
Stecher B 801 Wagner M
Stecher B 801 Eichorst SA
Stecher B 801 Mussmann M
Stecher B 801 Wasmund K
Stecher B 801 Herbold CW
Stecher B 801 Sedlacek CJ
Clavel T 728 Horn M
Clavel T 728 Daims H
Clavel T 728 Berry D
Clavel T 728 Loy A
Clavel T 728 Wagner M
Clavel T 728 Eichorst SA
Clavel T 728 Mussmann M
Clavel T 728 Wasmund K
Clavel T 728 Herbold CW
Clavel T 728 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Palermo A 2112 Horn M
Palermo A 2112 Daims H
Palermo A 2112 Berry D
Palermo A 2112 Loy A
Palermo A 2112 Wagner M
Palermo A 2112 Eichorst SA
Palermo A 2112 Mussmann M
Palermo A 2112 Wasmund K
Palermo A 2112 Herbold CW
Palermo A 2112 Sedlacek CJ
Forsberg EM 2113 Horn M
Forsberg EM 2113 Daims H
Forsberg EM 2113 Berry D
Forsberg EM 2113 Loy A
Forsberg EM 2113 Wagner M
Forsberg EM 2113 Eichorst SA
Forsberg EM 2113 Mussmann M
Forsberg EM 2113 Wasmund K
Forsberg EM 2113 Herbold CW
Forsberg EM 2113 Sedlacek CJ
Warth B 2114 Horn M
Warth B 2114 Daims H
Warth B 2114 Berry D
Warth B 2114 Loy A
Warth B 2114 Wagner M
Warth B 2114 Eichorst SA
Warth B 2114 Mussmann M
Warth B 2114 Wasmund K
Warth B 2114 Herbold CW
Warth B 2114 Sedlacek CJ
Aisporna AE 2115 Horn M
Aisporna AE 2115 Daims H
Aisporna AE 2115 Berry D
Aisporna AE 2115 Loy A
Aisporna AE 2115 Wagner M
Aisporna AE 2115 Eichorst SA
Aisporna AE 2115 Mussmann M
Aisporna AE 2115 Wasmund K
Aisporna AE 2115 Herbold CW
Aisporna AE 2115 Sedlacek CJ
Billings E 2116 Horn M
Billings E 2116 Daims H
Billings E 2116 Berry D
Billings E 2116 Loy A
Billings E 2116 Wagner M
Billings E 2116 Eichorst SA
Billings E 2116 Mussmann M
Billings E 2116 Wasmund K
Billings E 2116 Herbold CW
Billings E 2116 Sedlacek CJ
Kuang E 2117 Horn M
Kuang E 2117 Daims H
Kuang E 2117 Berry D
Kuang E 2117 Loy A
Kuang E 2117 Wagner M
Kuang E 2117 Eichorst SA
Kuang E 2117 Mussmann M
Kuang E 2117 Wasmund K
Kuang E 2117 Herbold CW
Kuang E 2117 Sedlacek CJ
Benton HP 2118 Horn M
Benton HP 2118 Daims H
Benton HP 2118 Berry D
Benton HP 2118 Loy A
Benton HP 2118 Wagner M
Benton HP 2118 Eichorst SA
Benton HP 2118 Mussmann M
Benton HP 2118 Wasmund K
Benton HP 2118 Herbold CW
Benton HP 2118 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Siuzdak G 2119 Horn M
Siuzdak G 2119 Daims H
Siuzdak G 2119 Berry D
Siuzdak G 2119 Loy A
Siuzdak G 2119 Wagner M
Siuzdak G 2119 Eichorst SA
Siuzdak G 2119 Mussmann M
Siuzdak G 2119 Wasmund K
Siuzdak G 2119 Herbold CW
Siuzdak G 2119 Sedlacek CJ
Bjerg JT 2048 Horn M
Bjerg JT 2048 Daims H
Bjerg JT 2048 Berry D
Bjerg JT 2048 Loy A
Bjerg JT 2048 Wagner M
Bjerg JT 2048 Eichorst SA
Bjerg JT 2048 Mussmann M
Bjerg JT 2048 Wasmund K
Bjerg JT 2048 Herbold CW
Bjerg JT 2048 Sedlacek CJ
Boschker HTS 2049 Horn M
Boschker HTS 2049 Daims H
Boschker HTS 2049 Berry D
Boschker HTS 2049 Loy A
Boschker HTS 2049 Wagner M
Boschker HTS 2049 Eichorst SA
Boschker HTS 2049 Mussmann M
Boschker HTS 2049 Wasmund K
Boschker HTS 2049 Herbold CW
Boschker HTS 2049 Sedlacek CJ
Larsen S 2050 Horn M
Larsen S 2050 Daims H
Larsen S 2050 Berry D
Larsen S 2050 Loy A
Larsen S 2050 Wagner M
Larsen S 2050 Eichorst SA
Larsen S 2050 Mussmann M
Larsen S 2050 Wasmund K
Larsen S 2050 Herbold CW
Larsen S 2050 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Schmid M 468 Horn M
Schmid M 468 Daims H
Schmid M 468 Berry D
Schmid M 468 Loy A
Schmid M 468 Wagner M
Schmid M 468 Eichorst SA
Schmid M 468 Mussmann M
Schmid M 468 Wasmund K
Schmid M 468 Herbold CW
Schmid M 468 Sedlacek CJ
Millo D 2051 Horn M
Millo D 2051 Daims H
Millo D 2051 Berry D
Millo D 2051 Loy A
Millo D 2051 Wagner M
Millo D 2051 Eichorst SA
Millo D 2051 Mussmann M
Millo D 2051 Wasmund K
Millo D 2051 Herbold CW
Millo D 2051 Sedlacek CJ
Tataru P 2052 Horn M
Tataru P 2052 Daims H
Tataru P 2052 Berry D
Tataru P 2052 Loy A
Tataru P 2052 Wagner M
Tataru P 2052 Eichorst SA
Tataru P 2052 Mussmann M
Tataru P 2052 Wasmund K
Tataru P 2052 Herbold CW
Tataru P 2052 Sedlacek CJ
Meysman FJR 2053 Horn M
Meysman FJR 2053 Daims H
Meysman FJR 2053 Berry D
Meysman FJR 2053 Loy A
Meysman FJR 2053 Wagner M
Meysman FJR 2053 Eichorst SA
Meysman FJR 2053 Mussmann M
Meysman FJR 2053 Wasmund K
Meysman FJR 2053 Herbold CW
Meysman FJR 2053 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Nielsen LP 2054 Horn M
Nielsen LP 2054 Daims H
Nielsen LP 2054 Berry D
Nielsen LP 2054 Loy A
Nielsen LP 2054 Wagner M
Nielsen LP 2054 Eichorst SA
Nielsen LP 2054 Mussmann M
Nielsen LP 2054 Wasmund K
Nielsen LP 2054 Herbold CW
Nielsen LP 2054 Sedlacek CJ
Schramm A 453 Horn M
Schramm A 453 Daims H
Schramm A 453 Berry D
Schramm A 453 Loy A
Schramm A 453 Wagner M
Schramm A 453 Eichorst SA
Schramm A 453 Mussmann M
Schramm A 453 Wasmund K
Schramm A 453 Herbold CW
Schramm A 453 Sedlacek CJ
Hubalek V 1966 Horn M
Hubalek V 1966 Daims H
Hubalek V 1966 Berry D
Hubalek V 1966 Loy A
Hubalek V 1966 Wagner M
Hubalek V 1966 Eichorst SA
Hubalek V 1966 Mussmann M
Hubalek V 1966 Wasmund K
Hubalek V 1966 Herbold CW
Hubalek V 1966 Sedlacek CJ
Buck M 1967 Horn M
Buck M 1967 Daims H
Buck M 1967 Berry D
Buck M 1967 Loy A
Buck M 1967 Wagner M
Buck M 1967 Eichorst SA
Buck M 1967 Mussmann M
Buck M 1967 Wasmund K
Buck M 1967 Herbold CW
Buck M 1967 Sedlacek CJ
Tan B 1968 Horn M
Tan B 1968 Daims H
Tan B 1968 Berry D
Tan B 1968 Loy A
Tan B 1968 Wagner M
Tan B 1968 Eichorst SA
Tan B 1968 Mussmann M
Tan B 1968 Wasmund K
Tan B 1968 Herbold CW
Tan B 1968 Sedlacek CJ
Foght J 1969 Horn M
Foght J 1969 Daims H
Foght J 1969 Berry D
Foght J 1969 Loy A
Foght J 1969 Wagner M
Foght J 1969 Eichorst SA
Foght J 1969 Mussmann M
Foght J 1969 Wasmund K
Foght J 1969 Herbold CW
Foght J 1969 Sedlacek CJ
Wendeberg A 1970 Horn M
Wendeberg A 1970 Daims H
Wendeberg A 1970 Berry D
Wendeberg A 1970 Loy A
Wendeberg A 1970 Wagner M
Wendeberg A 1970 Eichorst SA
Wendeberg A 1970 Mussmann M
Wendeberg A 1970 Wasmund K
Wendeberg A 1970 Herbold CW
Wendeberg A 1970 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Bertilsson S 1971 Horn M
Bertilsson S 1971 Daims H
Bertilsson S 1971 Berry D
Bertilsson S 1971 Loy A
Bertilsson S 1971 Wagner M
Bertilsson S 1971 Eichorst SA
Bertilsson S 1971 Mussmann M
Bertilsson S 1971 Wasmund K
Bertilsson S 1971 Herbold CW
Bertilsson S 1971 Sedlacek CJ
Eiler A 1972 Horn M
Eiler A 1972 Daims H
Eiler A 1972 Berry D
Eiler A 1972 Loy A
Eiler A 1972 Wagner M
Eiler A 1972 Eichorst SA
Eiler A 1972 Mussmann M
Eiler A 1972 Wasmund K
Eiler A 1972 Herbold CW
Eiler A 1972 Sedlacek CJ
Lesaulnier CC 1920 Horn M
Lesaulnier CC 1920 Daims H
Lesaulnier CC 1920 Berry D
Lesaulnier CC 1920 Loy A
Lesaulnier CC 1920 Wagner M
Lesaulnier CC 1920 Eichorst SA
Lesaulnier CC 1920 Mussmann M
Lesaulnier CC 1920 Wasmund K
Lesaulnier CC 1920 Herbold CW
Lesaulnier CC 1920 Sedlacek CJ
Herbold CW 321 Horn M
Herbold CW 321 Daims H
Herbold CW 321 Berry D
Herbold CW 321 Loy A
Herbold CW 321 Wagner M
Herbold CW 321 Eichorst SA
Herbold CW 321 Mussmann M
Herbold CW 321 Wasmund K
Herbold CW 321 Herbold CW
Herbold CW 321 Sedlacek CJ
Pelikan C 322 Horn M
Pelikan C 322 Daims H
Pelikan C 322 Berry D
Pelikan C 322 Loy A
Pelikan C 322 Wagner M
Pelikan C 322 Eichorst SA
Pelikan C 322 Mussmann M
Pelikan C 322 Wasmund K
Pelikan C 322 Herbold CW
Pelikan C 322 Sedlacek CJ
Gérard C 1921 Horn M
Gérard C 1921 Daims H
Gérard C 1921 Berry D
Gérard C 1921 Loy A
Gérard C 1921 Wagner M
Gérard C 1921 Eichorst SA
Gérard C 1921 Mussmann M
Gérard C 1921 Wasmund K
Gérard C 1921 Herbold CW
Gérard C 1921 Sedlacek CJ
Le Coz X 1922 Horn M
Le Coz X 1922 Daims H
Le Coz X 1922 Berry D
Le Coz X 1922 Loy A
Le Coz X 1922 Wagner M
Le Coz X 1922 Eichorst SA
Le Coz X 1922 Mussmann M
Le Coz X 1922 Wasmund K
Le Coz X 1922 Herbold CW
Le Coz X 1922 Sedlacek CJ
Gagnot S 1923 Horn M
Gagnot S 1923 Daims H
Gagnot S 1923 Berry D
Gagnot S 1923 Loy A
Gagnot S 1923 Wagner M
Gagnot S 1923 Eichorst SA
Gagnot S 1923 Mussmann M
Gagnot S 1923 Wasmund K
Gagnot S 1923 Herbold CW
Gagnot S 1923 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Niggemann J 1924 Horn M
Niggemann J 1924 Daims H
Niggemann J 1924 Berry D
Niggemann J 1924 Loy A
Niggemann J 1924 Wagner M
Niggemann J 1924 Eichorst SA
Niggemann J 1924 Mussmann M
Niggemann J 1924 Wasmund K
Niggemann J 1924 Herbold CW
Niggemann J 1924 Sedlacek CJ
Dittmar T 1925 Horn M
Dittmar T 1925 Daims H
Dittmar T 1925 Berry D
Dittmar T 1925 Loy A
Dittmar T 1925 Wagner M
Dittmar T 1925 Eichorst SA
Dittmar T 1925 Mussmann M
Dittmar T 1925 Wasmund K
Dittmar T 1925 Herbold CW
Dittmar T 1925 Sedlacek CJ
Singer GA 1153 Horn M
Singer GA 1153 Daims H
Singer GA 1153 Berry D
Singer GA 1153 Loy A
Singer GA 1153 Wagner M
Singer GA 1153 Eichorst SA
Singer GA 1153 Mussmann M
Singer GA 1153 Wasmund K
Singer GA 1153 Herbold CW
Singer GA 1153 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Ladurner A 1960 Horn M
Ladurner A 1960 Daims H
Ladurner A 1960 Berry D
Ladurner A 1960 Loy A
Ladurner A 1960 Wagner M
Ladurner A 1960 Eichorst SA
Ladurner A 1960 Mussmann M
Ladurner A 1960 Wasmund K
Ladurner A 1960 Herbold CW
Ladurner A 1960 Sedlacek CJ
Zehl M 1316 Horn M
Zehl M 1316 Daims H
Zehl M 1316 Berry D
Zehl M 1316 Loy A
Zehl M 1316 Wagner M
Zehl M 1316 Eichorst SA
Zehl M 1316 Mussmann M
Zehl M 1316 Wasmund K
Zehl M 1316 Herbold CW
Zehl M 1316 Sedlacek CJ
Grienke U 1961 Horn M
Grienke U 1961 Daims H
Grienke U 1961 Berry D
Grienke U 1961 Loy A
Grienke U 1961 Wagner M
Grienke U 1961 Eichorst SA
Grienke U 1961 Mussmann M
Grienke U 1961 Wasmund K
Grienke U 1961 Herbold CW
Grienke U 1961 Sedlacek CJ
Hofstadler C 1962 Horn M
Hofstadler C 1962 Daims H
Hofstadler C 1962 Berry D
Hofstadler C 1962 Loy A
Hofstadler C 1962 Wagner M
Hofstadler C 1962 Eichorst SA
Hofstadler C 1962 Mussmann M
Hofstadler C 1962 Wasmund K
Hofstadler C 1962 Herbold CW
Hofstadler C 1962 Sedlacek CJ
Faur N 1963 Horn M
Faur N 1963 Daims H
Faur N 1963 Berry D
Faur N 1963 Loy A
Faur N 1963 Wagner M
Faur N 1963 Eichorst SA
Faur N 1963 Mussmann M
Faur N 1963 Wasmund K
Faur N 1963 Herbold CW
Faur N 1963 Sedlacek CJ
Pereira FC 1844 Horn M
Pereira FC 1844 Daims H
Pereira FC 1844 Berry D
Pereira FC 1844 Loy A
Pereira FC 1844 Wagner M
Pereira FC 1844 Eichorst SA
Pereira FC 1844 Mussmann M
Pereira FC 1844 Wasmund K
Pereira FC 1844 Herbold CW
Pereira FC 1844 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Dirsch VM 1964 Horn M
Dirsch VM 1964 Daims H
Dirsch VM 1964 Berry D
Dirsch VM 1964 Loy A
Dirsch VM 1964 Wagner M
Dirsch VM 1964 Eichorst SA
Dirsch VM 1964 Mussmann M
Dirsch VM 1964 Wasmund K
Dirsch VM 1964 Herbold CW
Dirsch VM 1964 Sedlacek CJ
Rollinger JM 1965 Horn M
Rollinger JM 1965 Daims H
Rollinger JM 1965 Berry D
Rollinger JM 1965 Loy A
Rollinger JM 1965 Wagner M
Rollinger JM 1965 Eichorst SA
Rollinger JM 1965 Mussmann M
Rollinger JM 1965 Wasmund K
Rollinger JM 1965 Herbold CW
Rollinger JM 1965 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Gutierrez T 642 Horn M
Gutierrez T 642 Daims H
Gutierrez T 642 Berry D
Gutierrez T 642 Loy A
Gutierrez T 642 Wagner M
Gutierrez T 642 Eichorst SA
Gutierrez T 642 Mussmann M
Gutierrez T 642 Wasmund K
Gutierrez T 642 Herbold CW
Gutierrez T 642 Sedlacek CJ
Harrison JP 1767 Horn M
Harrison JP 1767 Daims H
Harrison JP 1767 Berry D
Harrison JP 1767 Loy A
Harrison JP 1767 Wagner M
Harrison JP 1767 Eichorst SA
Harrison JP 1767 Mussmann M
Harrison JP 1767 Wasmund K
Harrison JP 1767 Herbold CW
Harrison JP 1767 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Lang M 1874 Horn M
Lang M 1874 Daims H
Lang M 1874 Berry D
Lang M 1874 Loy A
Lang M 1874 Wagner M
Lang M 1874 Eichorst SA
Lang M 1874 Mussmann M
Lang M 1874 Wasmund K
Lang M 1874 Herbold CW
Lang M 1874 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Passecker K 1875 Horn M
Passecker K 1875 Daims H
Passecker K 1875 Berry D
Passecker K 1875 Loy A
Passecker K 1875 Wagner M
Passecker K 1875 Eichorst SA
Passecker K 1875 Mussmann M
Passecker K 1875 Wasmund K
Passecker K 1875 Herbold CW
Passecker K 1875 Sedlacek CJ
Mesteri I 1876 Horn M
Mesteri I 1876 Daims H
Mesteri I 1876 Berry D
Mesteri I 1876 Loy A
Mesteri I 1876 Wagner M
Mesteri I 1876 Eichorst SA
Mesteri I 1876 Mussmann M
Mesteri I 1876 Wasmund K
Mesteri I 1876 Herbold CW
Mesteri I 1876 Sedlacek CJ
Bhuju S 1877 Horn M
Bhuju S 1877 Daims H
Bhuju S 1877 Berry D
Bhuju S 1877 Loy A
Bhuju S 1877 Wagner M
Bhuju S 1877 Eichorst SA
Bhuju S 1877 Mussmann M
Bhuju S 1877 Wasmund K
Bhuju S 1877 Herbold CW
Bhuju S 1877 Sedlacek CJ
Ebner F 1878 Horn M
Ebner F 1878 Daims H
Ebner F 1878 Berry D
Ebner F 1878 Loy A
Ebner F 1878 Wagner M
Ebner F 1878 Eichorst SA
Ebner F 1878 Mussmann M
Ebner F 1878 Wasmund K
Ebner F 1878 Herbold CW
Ebner F 1878 Sedlacek CJ
Sedlyarov V 1879 Horn M
Sedlyarov V 1879 Daims H
Sedlyarov V 1879 Berry D
Sedlyarov V 1879 Loy A
Sedlyarov V 1879 Wagner M
Sedlyarov V 1879 Eichorst SA
Sedlyarov V 1879 Mussmann M
Sedlyarov V 1879 Wasmund K
Sedlyarov V 1879 Herbold CW
Sedlyarov V 1879 Sedlacek CJ
Evstatiev R 1880 Horn M
Evstatiev R 1880 Daims H
Evstatiev R 1880 Berry D
Evstatiev R 1880 Loy A
Evstatiev R 1880 Wagner M
Evstatiev R 1880 Eichorst SA
Evstatiev R 1880 Mussmann M
Evstatiev R 1880 Wasmund K
Evstatiev R 1880 Herbold CW
Evstatiev R 1880 Sedlacek CJ
Dammann K 1881 Horn M
Dammann K 1881 Daims H
Dammann K 1881 Berry D
Dammann K 1881 Loy A
Dammann K 1881 Wagner M
Dammann K 1881 Eichorst SA
Dammann K 1881 Mussmann M
Dammann K 1881 Wasmund K
Dammann K 1881 Herbold CW
Dammann K 1881 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Kuzyk O 323 Horn M
Kuzyk O 323 Daims H
Kuzyk O 323 Berry D
Kuzyk O 323 Loy A
Kuzyk O 323 Wagner M
Kuzyk O 323 Eichorst SA
Kuzyk O 323 Mussmann M
Kuzyk O 323 Wasmund K
Kuzyk O 323 Herbold CW
Kuzyk O 323 Sedlacek CJ
Kovarik P 1882 Horn M
Kovarik P 1882 Daims H
Kovarik P 1882 Berry D
Kovarik P 1882 Loy A
Kovarik P 1882 Wagner M
Kovarik P 1882 Eichorst SA
Kovarik P 1882 Mussmann M
Kovarik P 1882 Wasmund K
Kovarik P 1882 Herbold CW
Kovarik P 1882 Sedlacek CJ
Khare V 1883 Horn M
Khare V 1883 Daims H
Khare V 1883 Berry D
Khare V 1883 Loy A
Khare V 1883 Wagner M
Khare V 1883 Eichorst SA
Khare V 1883 Mussmann M
Khare V 1883 Wasmund K
Khare V 1883 Herbold CW
Khare V 1883 Sedlacek CJ
Beibel M 1884 Horn M
Beibel M 1884 Daims H
Beibel M 1884 Berry D
Beibel M 1884 Loy A
Beibel M 1884 Wagner M
Beibel M 1884 Eichorst SA
Beibel M 1884 Mussmann M
Beibel M 1884 Wasmund K
Beibel M 1884 Herbold CW
Beibel M 1884 Sedlacek CJ
Roma G 1885 Horn M
Roma G 1885 Daims H
Roma G 1885 Berry D
Roma G 1885 Loy A
Roma G 1885 Wagner M
Roma G 1885 Eichorst SA
Roma G 1885 Mussmann M
Roma G 1885 Wasmund K
Roma G 1885 Herbold CW
Roma G 1885 Sedlacek CJ
Meisner-Kober N 1886 Horn M
Meisner-Kober N 1886 Daims H
Meisner-Kober N 1886 Berry D
Meisner-Kober N 1886 Loy A
Meisner-Kober N 1886 Wagner M
Meisner-Kober N 1886 Eichorst SA
Meisner-Kober N 1886 Mussmann M
Meisner-Kober N 1886 Wasmund K
Meisner-Kober N 1886 Herbold CW
Meisner-Kober N 1886 Sedlacek CJ
Gasche C 1888 Horn M
Gasche C 1888 Daims H
Gasche C 1888 Berry D
Gasche C 1888 Loy A
Gasche C 1888 Wagner M
Gasche C 1888 Eichorst SA
Gasche C 1888 Mussmann M
Gasche C 1888 Wasmund K
Gasche C 1888 Herbold CW
Gasche C 1888 Sedlacek CJ
Schueller K 1891 Horn M
Schueller K 1891 Daims H
Schueller K 1891 Berry D
Schueller K 1891 Loy A
Schueller K 1891 Wagner M
Schueller K 1891 Eichorst SA
Schueller K 1891 Mussmann M
Schueller K 1891 Wasmund K
Schueller K 1891 Herbold CW
Schueller K 1891 Sedlacek CJ
Riva A 1551 Horn M
Riva A 1551 Daims H
Riva A 1551 Berry D
Riva A 1551 Loy A
Riva A 1551 Wagner M
Riva A 1551 Eichorst SA
Riva A 1551 Mussmann M
Riva A 1551 Wasmund K
Riva A 1551 Herbold CW
Riva A 1551 Sedlacek CJ
Pfeiffer S 965 Horn M
Pfeiffer S 965 Daims H
Pfeiffer S 965 Berry D
Pfeiffer S 965 Loy A
Pfeiffer S 965 Wagner M
Pfeiffer S 965 Eichorst SA
Pfeiffer S 965 Mussmann M
Pfeiffer S 965 Wasmund K
Pfeiffer S 965 Herbold CW
Pfeiffer S 965 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Somoza V 1857 Horn M
Somoza V 1857 Daims H
Somoza V 1857 Berry D
Somoza V 1857 Loy A
Somoza V 1857 Wagner M
Somoza V 1857 Eichorst SA
Somoza V 1857 Mussmann M
Somoza V 1857 Wasmund K
Somoza V 1857 Herbold CW
Somoza V 1857 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Pfann C 1727 Horn M
Pfann C 1727 Daims H
Pfann C 1727 Berry D
Pfann C 1727 Loy A
Pfann C 1727 Wagner M
Pfann C 1727 Eichorst SA
Pfann C 1727 Mussmann M
Pfann C 1727 Wasmund K
Pfann C 1727 Herbold CW
Pfann C 1727 Sedlacek CJ
Steinberger M 1837 Horn M
Steinberger M 1837 Daims H
Steinberger M 1837 Berry D
Steinberger M 1837 Loy A
Steinberger M 1837 Wagner M
Steinberger M 1837 Eichorst SA
Steinberger M 1837 Mussmann M
Steinberger M 1837 Wasmund K
Steinberger M 1837 Herbold CW
Steinberger M 1837 Sedlacek CJ
Hanson B 1425 Horn M
Hanson B 1425 Daims H
Hanson B 1425 Berry D
Hanson B 1425 Loy A
Hanson B 1425 Wagner M
Hanson B 1425 Eichorst SA
Hanson B 1425 Mussmann M
Hanson B 1425 Wasmund K
Hanson B 1425 Herbold CW
Hanson B 1425 Sedlacek CJ
Herp S 1732 Horn M
Herp S 1732 Daims H
Herp S 1732 Berry D
Herp S 1732 Loy A
Herp S 1732 Wagner M
Herp S 1732 Eichorst SA
Herp S 1732 Mussmann M
Herp S 1732 Wasmund K
Herp S 1732 Herbold CW
Herp S 1732 Sedlacek CJ
Brugiroux S 803 Horn M
Brugiroux S 803 Daims H
Brugiroux S 803 Berry D
Brugiroux S 803 Loy A
Brugiroux S 803 Wagner M
Brugiroux S 803 Eichorst SA
Brugiroux S 803 Mussmann M
Brugiroux S 803 Wasmund K
Brugiroux S 803 Herbold CW
Brugiroux S 803 Sedlacek CJ
Gomes Neto JC 1838 Horn M
Gomes Neto JC 1838 Daims H
Gomes Neto JC 1838 Berry D
Gomes Neto JC 1838 Loy A
Gomes Neto JC 1838 Wagner M
Gomes Neto JC 1838 Eichorst SA
Gomes Neto JC 1838 Mussmann M
Gomes Neto JC 1838 Wasmund K
Gomes Neto JC 1838 Herbold CW
Gomes Neto JC 1838 Sedlacek CJ
Boekschoten MV 1839 Horn M
Boekschoten MV 1839 Daims H
Boekschoten MV 1839 Berry D
Boekschoten MV 1839 Loy A
Boekschoten MV 1839 Wagner M
Boekschoten MV 1839 Eichorst SA
Boekschoten MV 1839 Mussmann M
Boekschoten MV 1839 Wasmund K
Boekschoten MV 1839 Herbold CW
Boekschoten MV 1839 Sedlacek CJ
Schwab C 513 Horn M
Schwab C 513 Daims H
Schwab C 513 Berry D
Schwab C 513 Loy A
Schwab C 513 Wagner M
Schwab C 513 Eichorst SA
Schwab C 513 Mussmann M
Schwab C 513 Wasmund K
Schwab C 513 Herbold CW
Schwab C 513 Sedlacek CJ
Urich T 515 Horn M
Urich T 515 Daims H
Urich T 515 Berry D
Urich T 515 Loy A
Urich T 515 Wagner M
Urich T 515 Eichorst SA
Urich T 515 Mussmann M
Urich T 515 Wasmund K
Urich T 515 Herbold CW
Urich T 515 Sedlacek CJ
Ramer-Tait AE 1840 Horn M
Ramer-Tait AE 1840 Daims H
Ramer-Tait AE 1840 Berry D
Ramer-Tait AE 1840 Loy A
Ramer-Tait AE 1840 Wagner M
Ramer-Tait AE 1840 Eichorst SA
Ramer-Tait AE 1840 Mussmann M
Ramer-Tait AE 1840 Wasmund K
Ramer-Tait AE 1840 Herbold CW
Ramer-Tait AE 1840 Sedlacek CJ
Rattei T 307 Horn M
Rattei T 307 Daims H
Rattei T 307 Berry D
Rattei T 307 Loy A
Rattei T 307 Wagner M
Rattei T 307 Eichorst SA
Rattei T 307 Mussmann M
Rattei T 307 Wasmund K
Rattei T 307 Herbold CW
Rattei T 307 Sedlacek CJ
Stecher B 801 Horn M
Stecher B 801 Daims H
Stecher B 801 Berry D
Stecher B 801 Loy A
Stecher B 801 Wagner M
Stecher B 801 Eichorst SA
Stecher B 801 Mussmann M
Stecher B 801 Wasmund K
Stecher B 801 Herbold CW
Stecher B 801 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Pereira FC 1844 Horn M
Pereira FC 1844 Daims H
Pereira FC 1844 Berry D
Pereira FC 1844 Loy A
Pereira FC 1844 Wagner M
Pereira FC 1844 Eichorst SA
Pereira FC 1844 Mussmann M
Pereira FC 1844 Wasmund K
Pereira FC 1844 Herbold CW
Pereira FC 1844 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Hochkogler CM 1846 Horn M
Hochkogler CM 1846 Daims H
Hochkogler CM 1846 Berry D
Hochkogler CM 1846 Loy A
Hochkogler CM 1846 Wagner M
Hochkogler CM 1846 Eichorst SA
Hochkogler CM 1846 Mussmann M
Hochkogler CM 1846 Wasmund K
Hochkogler CM 1846 Herbold CW
Hochkogler CM 1846 Sedlacek CJ
Lieder B 1847 Horn M
Lieder B 1847 Daims H
Lieder B 1847 Berry D
Lieder B 1847 Loy A
Lieder B 1847 Wagner M
Lieder B 1847 Eichorst SA
Lieder B 1847 Mussmann M
Lieder B 1847 Wasmund K
Lieder B 1847 Herbold CW
Lieder B 1847 Sedlacek CJ
Rust P 1848 Horn M
Rust P 1848 Daims H
Rust P 1848 Berry D
Rust P 1848 Loy A
Rust P 1848 Wagner M
Rust P 1848 Eichorst SA
Rust P 1848 Mussmann M
Rust P 1848 Wasmund K
Rust P 1848 Herbold CW
Rust P 1848 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Meier SM 1849 Horn M
Meier SM 1849 Daims H
Meier SM 1849 Berry D
Meier SM 1849 Loy A
Meier SM 1849 Wagner M
Meier SM 1849 Eichorst SA
Meier SM 1849 Mussmann M
Meier SM 1849 Wasmund K
Meier SM 1849 Herbold CW
Meier SM 1849 Sedlacek CJ
Pignitter M 1850 Horn M
Pignitter M 1850 Daims H
Pignitter M 1850 Berry D
Pignitter M 1850 Loy A
Pignitter M 1850 Wagner M
Pignitter M 1850 Eichorst SA
Pignitter M 1850 Mussmann M
Pignitter M 1850 Wasmund K
Pignitter M 1850 Herbold CW
Pignitter M 1850 Sedlacek CJ
Riva A 1551 Horn M
Riva A 1551 Daims H
Riva A 1551 Berry D
Riva A 1551 Loy A
Riva A 1551 Wagner M
Riva A 1551 Eichorst SA
Riva A 1551 Mussmann M
Riva A 1551 Wasmund K
Riva A 1551 Herbold CW
Riva A 1551 Sedlacek CJ
Leitinger A 1851 Horn M
Leitinger A 1851 Daims H
Leitinger A 1851 Berry D
Leitinger A 1851 Loy A
Leitinger A 1851 Wagner M
Leitinger A 1851 Eichorst SA
Leitinger A 1851 Mussmann M
Leitinger A 1851 Wasmund K
Leitinger A 1851 Herbold CW
Leitinger A 1851 Sedlacek CJ
Bruk A 1852 Horn M
Bruk A 1852 Daims H
Bruk A 1852 Berry D
Bruk A 1852 Loy A
Bruk A 1852 Wagner M
Bruk A 1852 Eichorst SA
Bruk A 1852 Mussmann M
Bruk A 1852 Wasmund K
Bruk A 1852 Herbold CW
Bruk A 1852 Sedlacek CJ
Wagner S 1853 Horn M
Wagner S 1853 Daims H
Wagner S 1853 Berry D
Wagner S 1853 Loy A
Wagner S 1853 Wagner M
Wagner S 1853 Eichorst SA
Wagner S 1853 Mussmann M
Wagner S 1853 Wasmund K
Wagner S 1853 Herbold CW
Wagner S 1853 Sedlacek CJ
Hans J 1854 Horn M
Hans J 1854 Daims H
Hans J 1854 Berry D
Hans J 1854 Loy A
Hans J 1854 Wagner M
Hans J 1854 Eichorst SA
Hans J 1854 Mussmann M
Hans J 1854 Wasmund K
Hans J 1854 Herbold CW
Hans J 1854 Sedlacek CJ
Widder S 662 Horn M
Widder S 662 Daims H
Widder S 662 Berry D
Widder S 662 Loy A
Widder S 662 Wagner M
Widder S 662 Eichorst SA
Widder S 662 Mussmann M
Widder S 662 Wasmund K
Widder S 662 Herbold CW
Widder S 662 Sedlacek CJ
Ley JP 1855 Horn M
Ley JP 1855 Daims H
Ley JP 1855 Berry D
Ley JP 1855 Loy A
Ley JP 1855 Wagner M
Ley JP 1855 Eichorst SA
Ley JP 1855 Mussmann M
Ley JP 1855 Wasmund K
Ley JP 1855 Herbold CW
Ley JP 1855 Sedlacek CJ
Krammer GE 1856 Horn M
Krammer GE 1856 Daims H
Krammer GE 1856 Berry D
Krammer GE 1856 Loy A
Krammer GE 1856 Wagner M
Krammer GE 1856 Eichorst SA
Krammer GE 1856 Mussmann M
Krammer GE 1856 Wasmund K
Krammer GE 1856 Herbold CW
Krammer GE 1856 Sedlacek CJ
Somoza V 1857 Horn M
Somoza V 1857 Daims H
Somoza V 1857 Berry D
Somoza V 1857 Loy A
Somoza V 1857 Wagner M
Somoza V 1857 Eichorst SA
Somoza V 1857 Mussmann M
Somoza V 1857 Wasmund K
Somoza V 1857 Herbold CW
Somoza V 1857 Sedlacek CJ
Riva A 1551 Horn M
Riva A 1551 Daims H
Riva A 1551 Berry D
Riva A 1551 Loy A
Riva A 1551 Wagner M
Riva A 1551 Eichorst SA
Riva A 1551 Mussmann M
Riva A 1551 Wasmund K
Riva A 1551 Herbold CW
Riva A 1551 Sedlacek CJ
Borgo F 1552 Horn M
Borgo F 1552 Daims H
Borgo F 1552 Berry D
Borgo F 1552 Loy A
Borgo F 1552 Wagner M
Borgo F 1552 Eichorst SA
Borgo F 1552 Mussmann M
Borgo F 1552 Wasmund K
Borgo F 1552 Herbold CW
Borgo F 1552 Sedlacek CJ
Lassandro C 1553 Horn M
Lassandro C 1553 Daims H
Lassandro C 1553 Berry D
Lassandro C 1553 Loy A
Lassandro C 1553 Wagner M
Lassandro C 1553 Eichorst SA
Lassandro C 1553 Mussmann M
Lassandro C 1553 Wasmund K
Lassandro C 1553 Herbold CW
Lassandro C 1553 Sedlacek CJ
Verduci E 1554 Horn M
Verduci E 1554 Daims H
Verduci E 1554 Berry D
Verduci E 1554 Loy A
Verduci E 1554 Wagner M
Verduci E 1554 Eichorst SA
Verduci E 1554 Mussmann M
Verduci E 1554 Wasmund K
Verduci E 1554 Herbold CW
Verduci E 1554 Sedlacek CJ
Morace G 1555 Horn M
Morace G 1555 Daims H
Morace G 1555 Berry D
Morace G 1555 Loy A
Morace G 1555 Wagner M
Morace G 1555 Eichorst SA
Morace G 1555 Mussmann M
Morace G 1555 Wasmund K
Morace G 1555 Herbold CW
Morace G 1555 Sedlacek CJ
Borghi E 1556 Horn M
Borghi E 1556 Daims H
Borghi E 1556 Berry D
Borghi E 1556 Loy A
Borghi E 1556 Wagner M
Borghi E 1556 Eichorst SA
Borghi E 1556 Mussmann M
Borghi E 1556 Wasmund K
Borghi E 1556 Herbold CW
Borghi E 1556 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Brugiroux S 803 Horn M
Brugiroux S 803 Daims H
Brugiroux S 803 Berry D
Brugiroux S 803 Loy A
Brugiroux S 803 Wagner M
Brugiroux S 803 Eichorst SA
Brugiroux S 803 Mussmann M
Brugiroux S 803 Wasmund K
Brugiroux S 803 Herbold CW
Brugiroux S 803 Sedlacek CJ
Beutler M 1726 Horn M
Beutler M 1726 Daims H
Beutler M 1726 Berry D
Beutler M 1726 Loy A
Beutler M 1726 Wagner M
Beutler M 1726 Eichorst SA
Beutler M 1726 Mussmann M
Beutler M 1726 Wasmund K
Beutler M 1726 Herbold CW
Beutler M 1726 Sedlacek CJ
Pfann C 1727 Horn M
Pfann C 1727 Daims H
Pfann C 1727 Berry D
Pfann C 1727 Loy A
Pfann C 1727 Wagner M
Pfann C 1727 Eichorst SA
Pfann C 1727 Mussmann M
Pfann C 1727 Wasmund K
Pfann C 1727 Herbold CW
Pfann C 1727 Sedlacek CJ
Garzetti D 1728 Horn M
Garzetti D 1728 Daims H
Garzetti D 1728 Berry D
Garzetti D 1728 Loy A
Garzetti D 1728 Wagner M
Garzetti D 1728 Eichorst SA
Garzetti D 1728 Mussmann M
Garzetti D 1728 Wasmund K
Garzetti D 1728 Herbold CW
Garzetti D 1728 Sedlacek CJ
Ruscheweyh H-J 1729 Horn M
Ruscheweyh H-J 1729 Daims H
Ruscheweyh H-J 1729 Berry D
Ruscheweyh H-J 1729 Loy A
Ruscheweyh H-J 1729 Wagner M
Ruscheweyh H-J 1729 Eichorst SA
Ruscheweyh H-J 1729 Mussmann M
Ruscheweyh H-J 1729 Wasmund K
Ruscheweyh H-J 1729 Herbold CW
Ruscheweyh H-J 1729 Sedlacek CJ
Ring D 1730 Horn M
Ring D 1730 Daims H
Ring D 1730 Berry D
Ring D 1730 Loy A
Ring D 1730 Wagner M
Ring D 1730 Eichorst SA
Ring D 1730 Mussmann M
Ring D 1730 Wasmund K
Ring D 1730 Herbold CW
Ring D 1730 Sedlacek CJ
Diehl M 1731 Horn M
Diehl M 1731 Daims H
Diehl M 1731 Berry D
Diehl M 1731 Loy A
Diehl M 1731 Wagner M
Diehl M 1731 Eichorst SA
Diehl M 1731 Mussmann M
Diehl M 1731 Wasmund K
Diehl M 1731 Herbold CW
Diehl M 1731 Sedlacek CJ
Herp S 1732 Horn M
Herp S 1732 Daims H
Herp S 1732 Berry D
Herp S 1732 Loy A
Herp S 1732 Wagner M
Herp S 1732 Eichorst SA
Herp S 1732 Mussmann M
Herp S 1732 Wasmund K
Herp S 1732 Herbold CW
Herp S 1732 Sedlacek CJ
Lötscher Y 1733 Horn M
Lötscher Y 1733 Daims H
Lötscher Y 1733 Berry D
Lötscher Y 1733 Loy A
Lötscher Y 1733 Wagner M
Lötscher Y 1733 Eichorst SA
Lötscher Y 1733 Mussmann M
Lötscher Y 1733 Wasmund K
Lötscher Y 1733 Herbold CW
Lötscher Y 1733 Sedlacek CJ
Hussain S 1734 Horn M
Hussain S 1734 Daims H
Hussain S 1734 Berry D
Hussain S 1734 Loy A
Hussain S 1734 Wagner M
Hussain S 1734 Eichorst SA
Hussain S 1734 Mussmann M
Hussain S 1734 Wasmund K
Hussain S 1734 Herbold CW
Hussain S 1734 Sedlacek CJ
Bunk B 1735 Horn M
Bunk B 1735 Daims H
Bunk B 1735 Berry D
Bunk B 1735 Loy A
Bunk B 1735 Wagner M
Bunk B 1735 Eichorst SA
Bunk B 1735 Mussmann M
Bunk B 1735 Wasmund K
Bunk B 1735 Herbold CW
Bunk B 1735 Sedlacek CJ
Pukall R 1736 Horn M
Pukall R 1736 Daims H
Pukall R 1736 Berry D
Pukall R 1736 Loy A
Pukall R 1736 Wagner M
Pukall R 1736 Eichorst SA
Pukall R 1736 Mussmann M
Pukall R 1736 Wasmund K
Pukall R 1736 Herbold CW
Pukall R 1736 Sedlacek CJ
Huson DH 1737 Horn M
Huson DH 1737 Daims H
Huson DH 1737 Berry D
Huson DH 1737 Loy A
Huson DH 1737 Wagner M
Huson DH 1737 Eichorst SA
Huson DH 1737 Mussmann M
Huson DH 1737 Wasmund K
Huson DH 1737 Herbold CW
Huson DH 1737 Sedlacek CJ
Münch PC 1738 Horn M
Münch PC 1738 Daims H
Münch PC 1738 Berry D
Münch PC 1738 Loy A
Münch PC 1738 Wagner M
Münch PC 1738 Eichorst SA
Münch PC 1738 Mussmann M
Münch PC 1738 Wasmund K
Münch PC 1738 Herbold CW
Münch PC 1738 Sedlacek CJ
McHardy AC 1739 Horn M
McHardy AC 1739 Daims H
McHardy AC 1739 Berry D
McHardy AC 1739 Loy A
McHardy AC 1739 Wagner M
McHardy AC 1739 Eichorst SA
McHardy AC 1739 Mussmann M
McHardy AC 1739 Wasmund K
McHardy AC 1739 Herbold CW
McHardy AC 1739 Sedlacek CJ
McCoy KD 1740 Horn M
McCoy KD 1740 Daims H
McCoy KD 1740 Berry D
McCoy KD 1740 Loy A
McCoy KD 1740 Wagner M
McCoy KD 1740 Eichorst SA
McCoy KD 1740 Mussmann M
McCoy KD 1740 Wasmund K
McCoy KD 1740 Herbold CW
McCoy KD 1740 Sedlacek CJ
Macpherson AJ 1741 Horn M
Macpherson AJ 1741 Daims H
Macpherson AJ 1741 Berry D
Macpherson AJ 1741 Loy A
Macpherson AJ 1741 Wagner M
Macpherson AJ 1741 Eichorst SA
Macpherson AJ 1741 Mussmann M
Macpherson AJ 1741 Wasmund K
Macpherson AJ 1741 Herbold CW
Macpherson AJ 1741 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Clavel T 728 Horn M
Clavel T 728 Daims H
Clavel T 728 Berry D
Clavel T 728 Loy A
Clavel T 728 Wagner M
Clavel T 728 Eichorst SA
Clavel T 728 Mussmann M
Clavel T 728 Wasmund K
Clavel T 728 Herbold CW
Clavel T 728 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Stecher B 801 Horn M
Stecher B 801 Daims H
Stecher B 801 Berry D
Stecher B 801 Loy A
Stecher B 801 Wagner M
Stecher B 801 Eichorst SA
Stecher B 801 Mussmann M
Stecher B 801 Wasmund K
Stecher B 801 Herbold CW
Stecher B 801 Sedlacek CJ
Ralls MW 1668 Horn M
Ralls MW 1668 Daims H
Ralls MW 1668 Berry D
Ralls MW 1668 Loy A
Ralls MW 1668 Wagner M
Ralls MW 1668 Eichorst SA
Ralls MW 1668 Mussmann M
Ralls MW 1668 Wasmund K
Ralls MW 1668 Herbold CW
Ralls MW 1668 Sedlacek CJ
Demehri FR 1669 Horn M
Demehri FR 1669 Daims H
Demehri FR 1669 Berry D
Demehri FR 1669 Loy A
Demehri FR 1669 Wagner M
Demehri FR 1669 Eichorst SA
Demehri FR 1669 Mussmann M
Demehri FR 1669 Wasmund K
Demehri FR 1669 Herbold CW
Demehri FR 1669 Sedlacek CJ
Feng Y 1670 Horn M
Feng Y 1670 Daims H
Feng Y 1670 Berry D
Feng Y 1670 Loy A
Feng Y 1670 Wagner M
Feng Y 1670 Eichorst SA
Feng Y 1670 Mussmann M
Feng Y 1670 Wasmund K
Feng Y 1670 Herbold CW
Feng Y 1670 Sedlacek CJ
Raskind S 1671 Horn M
Raskind S 1671 Daims H
Raskind S 1671 Berry D
Raskind S 1671 Loy A
Raskind S 1671 Wagner M
Raskind S 1671 Eichorst SA
Raskind S 1671 Mussmann M
Raskind S 1671 Wasmund K
Raskind S 1671 Herbold CW
Raskind S 1671 Sedlacek CJ
Ruan C 1672 Horn M
Ruan C 1672 Daims H
Ruan C 1672 Berry D
Ruan C 1672 Loy A
Ruan C 1672 Wagner M
Ruan C 1672 Eichorst SA
Ruan C 1672 Mussmann M
Ruan C 1672 Wasmund K
Ruan C 1672 Herbold CW
Ruan C 1672 Sedlacek CJ
Schintlmeister A 282 Horn M
Schintlmeister A 282 Daims H
Schintlmeister A 282 Berry D
Schintlmeister A 282 Loy A
Schintlmeister A 282 Wagner M
Schintlmeister A 282 Eichorst SA
Schintlmeister A 282 Mussmann M
Schintlmeister A 282 Wasmund K
Schintlmeister A 282 Herbold CW
Schintlmeister A 282 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Hanson B 1425 Horn M
Hanson B 1425 Daims H
Hanson B 1425 Berry D
Hanson B 1425 Loy A
Hanson B 1425 Wagner M
Hanson B 1425 Eichorst SA
Hanson B 1425 Mussmann M
Hanson B 1425 Wasmund K
Hanson B 1425 Herbold CW
Hanson B 1425 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Burant CF 1673 Horn M
Burant CF 1673 Daims H
Burant CF 1673 Berry D
Burant CF 1673 Loy A
Burant CF 1673 Wagner M
Burant CF 1673 Eichorst SA
Burant CF 1673 Mussmann M
Burant CF 1673 Wasmund K
Burant CF 1673 Herbold CW
Burant CF 1673 Sedlacek CJ
Teitelbaum DH 1674 Horn M
Teitelbaum DH 1674 Daims H
Teitelbaum DH 1674 Berry D
Teitelbaum DH 1674 Loy A
Teitelbaum DH 1674 Wagner M
Teitelbaum DH 1674 Eichorst SA
Teitelbaum DH 1674 Mussmann M
Teitelbaum DH 1674 Wasmund K
Teitelbaum DH 1674 Herbold CW
Teitelbaum DH 1674 Sedlacek CJ
Schreiber-Brynzak E 1423 Horn M
Schreiber-Brynzak E 1423 Daims H
Schreiber-Brynzak E 1423 Berry D
Schreiber-Brynzak E 1423 Loy A
Schreiber-Brynzak E 1423 Wagner M
Schreiber-Brynzak E 1423 Eichorst SA
Schreiber-Brynzak E 1423 Mussmann M
Schreiber-Brynzak E 1423 Wasmund K
Schreiber-Brynzak E 1423 Herbold CW
Schreiber-Brynzak E 1423 Sedlacek CJ
Pichler V 1424 Horn M
Pichler V 1424 Daims H
Pichler V 1424 Berry D
Pichler V 1424 Loy A
Pichler V 1424 Wagner M
Pichler V 1424 Eichorst SA
Pichler V 1424 Mussmann M
Pichler V 1424 Wasmund K
Pichler V 1424 Herbold CW
Pichler V 1424 Sedlacek CJ
Heffeter P 1308 Horn M
Heffeter P 1308 Daims H
Heffeter P 1308 Berry D
Heffeter P 1308 Loy A
Heffeter P 1308 Wagner M
Heffeter P 1308 Eichorst SA
Heffeter P 1308 Mussmann M
Heffeter P 1308 Wasmund K
Heffeter P 1308 Herbold CW
Heffeter P 1308 Sedlacek CJ
Hanson B 1425 Horn M
Hanson B 1425 Daims H
Hanson B 1425 Berry D
Hanson B 1425 Loy A
Hanson B 1425 Wagner M
Hanson B 1425 Eichorst SA
Hanson B 1425 Mussmann M
Hanson B 1425 Wasmund K
Hanson B 1425 Herbold CW
Hanson B 1425 Sedlacek CJ
Theiner S 1306 Horn M
Theiner S 1306 Daims H
Theiner S 1306 Berry D
Theiner S 1306 Loy A
Theiner S 1306 Wagner M
Theiner S 1306 Eichorst SA
Theiner S 1306 Mussmann M
Theiner S 1306 Wasmund K
Theiner S 1306 Herbold CW
Theiner S 1306 Sedlacek CJ
Lichtscheidl-Schultz I 1426 Horn M
Lichtscheidl-Schultz I 1426 Daims H
Lichtscheidl-Schultz I 1426 Berry D
Lichtscheidl-Schultz I 1426 Loy A
Lichtscheidl-Schultz I 1426 Wagner M
Lichtscheidl-Schultz I 1426 Eichorst SA
Lichtscheidl-Schultz I 1426 Mussmann M
Lichtscheidl-Schultz I 1426 Wasmund K
Lichtscheidl-Schultz I 1426 Herbold CW
Lichtscheidl-Schultz I 1426 Sedlacek CJ
Kornauth C 1427 Horn M
Kornauth C 1427 Daims H
Kornauth C 1427 Berry D
Kornauth C 1427 Loy A
Kornauth C 1427 Wagner M
Kornauth C 1427 Eichorst SA
Kornauth C 1427 Mussmann M
Kornauth C 1427 Wasmund K
Kornauth C 1427 Herbold CW
Kornauth C 1427 Sedlacek CJ
Bamonti L 1428 Horn M
Bamonti L 1428 Daims H
Bamonti L 1428 Berry D
Bamonti L 1428 Loy A
Bamonti L 1428 Wagner M
Bamonti L 1428 Eichorst SA
Bamonti L 1428 Mussmann M
Bamonti L 1428 Wasmund K
Bamonti L 1428 Herbold CW
Bamonti L 1428 Sedlacek CJ
Dhery V 1429 Horn M
Dhery V 1429 Daims H
Dhery V 1429 Berry D
Dhery V 1429 Loy A
Dhery V 1429 Wagner M
Dhery V 1429 Eichorst SA
Dhery V 1429 Mussmann M
Dhery V 1429 Wasmund K
Dhery V 1429 Herbold CW
Dhery V 1429 Sedlacek CJ
Groza D 1430 Horn M
Groza D 1430 Daims H
Groza D 1430 Berry D
Groza D 1430 Loy A
Groza D 1430 Wagner M
Groza D 1430 Eichorst SA
Groza D 1430 Mussmann M
Groza D 1430 Wasmund K
Groza D 1430 Herbold CW
Groza D 1430 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Berger W 1431 Horn M
Berger W 1431 Daims H
Berger W 1431 Berry D
Berger W 1431 Loy A
Berger W 1431 Wagner M
Berger W 1431 Eichorst SA
Berger W 1431 Mussmann M
Berger W 1431 Wasmund K
Berger W 1431 Herbold CW
Berger W 1431 Sedlacek CJ
Galanski M 588 Horn M
Galanski M 588 Daims H
Galanski M 588 Berry D
Galanski M 588 Loy A
Galanski M 588 Wagner M
Galanski M 588 Eichorst SA
Galanski M 588 Mussmann M
Galanski M 588 Wasmund K
Galanski M 588 Herbold CW
Galanski M 588 Sedlacek CJ
Jakupec MA 587 Horn M
Jakupec MA 587 Daims H
Jakupec MA 587 Berry D
Jakupec MA 587 Loy A
Jakupec MA 587 Wagner M
Jakupec MA 587 Eichorst SA
Jakupec MA 587 Mussmann M
Jakupec MA 587 Wasmund K
Jakupec MA 587 Herbold CW
Jakupec MA 587 Sedlacek CJ
Keppler BK 1313 Horn M
Keppler BK 1313 Daims H
Keppler BK 1313 Berry D
Keppler BK 1313 Loy A
Keppler BK 1313 Wagner M
Keppler BK 1313 Eichorst SA
Keppler BK 1313 Mussmann M
Keppler BK 1313 Wasmund K
Keppler BK 1313 Herbold CW
Keppler BK 1313 Sedlacek CJ
Robador A 388 Horn M
Robador A 388 Daims H
Robador A 388 Berry D
Robador A 388 Loy A
Robador A 388 Wagner M
Robador A 388 Eichorst SA
Robador A 388 Mussmann M
Robador A 388 Wasmund K
Robador A 388 Herbold CW
Robador A 388 Sedlacek CJ
Müller AL 358 Horn M
Müller AL 358 Daims H
Müller AL 358 Berry D
Müller AL 358 Loy A
Müller AL 358 Wagner M
Müller AL 358 Eichorst SA
Müller AL 358 Mussmann M
Müller AL 358 Wasmund K
Müller AL 358 Herbold CW
Müller AL 358 Sedlacek CJ
Sawicka JE 389 Horn M
Sawicka JE 389 Daims H
Sawicka JE 389 Berry D
Sawicka JE 389 Loy A
Sawicka JE 389 Wagner M
Sawicka JE 389 Eichorst SA
Sawicka JE 389 Mussmann M
Sawicka JE 389 Wasmund K
Sawicka JE 389 Herbold CW
Sawicka JE 389 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Hubert CRJ 390 Horn M
Hubert CRJ 390 Daims H
Hubert CRJ 390 Berry D
Hubert CRJ 390 Loy A
Hubert CRJ 390 Wagner M
Hubert CRJ 390 Eichorst SA
Hubert CRJ 390 Mussmann M
Hubert CRJ 390 Wasmund K
Hubert CRJ 390 Herbold CW
Hubert CRJ 390 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Jørgensen BB 362 Horn M
Jørgensen BB 362 Daims H
Jørgensen BB 362 Berry D
Jørgensen BB 362 Loy A
Jørgensen BB 362 Wagner M
Jørgensen BB 362 Eichorst SA
Jørgensen BB 362 Mussmann M
Jørgensen BB 362 Wasmund K
Jørgensen BB 362 Herbold CW
Jørgensen BB 362 Sedlacek CJ
Brüchert V 391 Horn M
Brüchert V 391 Daims H
Brüchert V 391 Berry D
Brüchert V 391 Loy A
Brüchert V 391 Wagner M
Brüchert V 391 Eichorst SA
Brüchert V 391 Mussmann M
Brüchert V 391 Wasmund K
Brüchert V 391 Herbold CW
Brüchert V 391 Sedlacek CJ
Mishamandani S 644 Horn M
Mishamandani S 644 Daims H
Mishamandani S 644 Berry D
Mishamandani S 644 Loy A
Mishamandani S 644 Wagner M
Mishamandani S 644 Eichorst SA
Mishamandani S 644 Mussmann M
Mishamandani S 644 Wasmund K
Mishamandani S 644 Herbold CW
Mishamandani S 644 Sedlacek CJ
Gutierrez T 642 Horn M
Gutierrez T 642 Daims H
Gutierrez T 642 Berry D
Gutierrez T 642 Loy A
Gutierrez T 642 Wagner M
Gutierrez T 642 Eichorst SA
Gutierrez T 642 Mussmann M
Gutierrez T 642 Wasmund K
Gutierrez T 642 Herbold CW
Gutierrez T 642 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Aitken MD 647 Horn M
Aitken MD 647 Daims H
Aitken MD 647 Berry D
Aitken MD 647 Loy A
Aitken MD 647 Wagner M
Aitken MD 647 Eichorst SA
Aitken MD 647 Mussmann M
Aitken MD 647 Wasmund K
Aitken MD 647 Herbold CW
Aitken MD 647 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Kuzyk O 323 Horn M
Kuzyk O 323 Daims H
Kuzyk O 323 Berry D
Kuzyk O 323 Loy A
Kuzyk O 323 Wagner M
Kuzyk O 323 Eichorst SA
Kuzyk O 323 Mussmann M
Kuzyk O 323 Wasmund K
Kuzyk O 323 Herbold CW
Kuzyk O 323 Sedlacek CJ
Rauch I 286 Horn M
Rauch I 286 Daims H
Rauch I 286 Berry D
Rauch I 286 Loy A
Rauch I 286 Wagner M
Rauch I 286 Eichorst SA
Rauch I 286 Mussmann M
Rauch I 286 Wasmund K
Rauch I 286 Herbold CW
Rauch I 286 Sedlacek CJ
Heider S 512 Horn M
Heider S 512 Daims H
Heider S 512 Berry D
Heider S 512 Loy A
Heider S 512 Wagner M
Heider S 512 Eichorst SA
Heider S 512 Mussmann M
Heider S 512 Wasmund K
Heider S 512 Herbold CW
Heider S 512 Sedlacek CJ
Schwab C 513 Horn M
Schwab C 513 Daims H
Schwab C 513 Berry D
Schwab C 513 Loy A
Schwab C 513 Wagner M
Schwab C 513 Eichorst SA
Schwab C 513 Mussmann M
Schwab C 513 Wasmund K
Schwab C 513 Herbold CW
Schwab C 513 Sedlacek CJ
Hainzl E 510 Horn M
Hainzl E 510 Daims H
Hainzl E 510 Berry D
Hainzl E 510 Loy A
Hainzl E 510 Wagner M
Hainzl E 510 Eichorst SA
Hainzl E 510 Mussmann M
Hainzl E 510 Wasmund K
Hainzl E 510 Herbold CW
Hainzl E 510 Sedlacek CJ
Decker T 287 Horn M
Decker T 287 Daims H
Decker T 287 Berry D
Decker T 287 Loy A
Decker T 287 Wagner M
Decker T 287 Eichorst SA
Decker T 287 Mussmann M
Decker T 287 Wasmund K
Decker T 287 Herbold CW
Decker T 287 Sedlacek CJ
Müller M 516 Horn M
Müller M 516 Daims H
Müller M 516 Berry D
Müller M 516 Loy A
Müller M 516 Wagner M
Müller M 516 Eichorst SA
Müller M 516 Mussmann M
Müller M 516 Wasmund K
Müller M 516 Herbold CW
Müller M 516 Sedlacek CJ
Strobl B 517 Horn M
Strobl B 517 Daims H
Strobl B 517 Berry D
Strobl B 517 Loy A
Strobl B 517 Wagner M
Strobl B 517 Eichorst SA
Strobl B 517 Mussmann M
Strobl B 517 Wasmund K
Strobl B 517 Herbold CW
Strobl B 517 Sedlacek CJ
Schleper C 405 Horn M
Schleper C 405 Daims H
Schleper C 405 Berry D
Schleper C 405 Loy A
Schleper C 405 Wagner M
Schleper C 405 Eichorst SA
Schleper C 405 Mussmann M
Schleper C 405 Wasmund K
Schleper C 405 Herbold CW
Schleper C 405 Sedlacek CJ
Urich T 515 Horn M
Urich T 515 Daims H
Urich T 515 Berry D
Urich T 515 Loy A
Urich T 515 Wagner M
Urich T 515 Eichorst SA
Urich T 515 Mussmann M
Urich T 515 Wasmund K
Urich T 515 Herbold CW
Urich T 515 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Kenner L 518 Horn M
Kenner L 518 Daims H
Kenner L 518 Berry D
Kenner L 518 Loy A
Kenner L 518 Wagner M
Kenner L 518 Eichorst SA
Kenner L 518 Mussmann M
Kenner L 518 Wasmund K
Kenner L 518 Herbold CW
Kenner L 518 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Hainzl E 510 Horn M
Hainzl E 510 Daims H
Hainzl E 510 Berry D
Hainzl E 510 Loy A
Hainzl E 510 Wagner M
Hainzl E 510 Eichorst SA
Hainzl E 510 Mussmann M
Hainzl E 510 Wasmund K
Hainzl E 510 Herbold CW
Hainzl E 510 Sedlacek CJ
Stockinger S 1266 Horn M
Stockinger S 1266 Daims H
Stockinger S 1266 Berry D
Stockinger S 1266 Loy A
Stockinger S 1266 Wagner M
Stockinger S 1266 Eichorst SA
Stockinger S 1266 Mussmann M
Stockinger S 1266 Wasmund K
Stockinger S 1266 Herbold CW
Stockinger S 1266 Sedlacek CJ
Rauch I 286 Horn M
Rauch I 286 Daims H
Rauch I 286 Berry D
Rauch I 286 Loy A
Rauch I 286 Wagner M
Rauch I 286 Eichorst SA
Rauch I 286 Mussmann M
Rauch I 286 Wasmund K
Rauch I 286 Herbold CW
Rauch I 286 Sedlacek CJ
Heider S 512 Horn M
Heider S 512 Daims H
Heider S 512 Berry D
Heider S 512 Loy A
Heider S 512 Wagner M
Heider S 512 Eichorst SA
Heider S 512 Mussmann M
Heider S 512 Wasmund K
Heider S 512 Herbold CW
Heider S 512 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Lassnig C 1267 Horn M
Lassnig C 1267 Daims H
Lassnig C 1267 Berry D
Lassnig C 1267 Loy A
Lassnig C 1267 Wagner M
Lassnig C 1267 Eichorst SA
Lassnig C 1267 Mussmann M
Lassnig C 1267 Wasmund K
Lassnig C 1267 Herbold CW
Lassnig C 1267 Sedlacek CJ
Schwab C 513 Horn M
Schwab C 513 Daims H
Schwab C 513 Berry D
Schwab C 513 Loy A
Schwab C 513 Wagner M
Schwab C 513 Eichorst SA
Schwab C 513 Mussmann M
Schwab C 513 Wasmund K
Schwab C 513 Herbold CW
Schwab C 513 Sedlacek CJ
Rosebrock F 511 Horn M
Rosebrock F 511 Daims H
Rosebrock F 511 Berry D
Rosebrock F 511 Loy A
Rosebrock F 511 Wagner M
Rosebrock F 511 Eichorst SA
Rosebrock F 511 Mussmann M
Rosebrock F 511 Wasmund K
Rosebrock F 511 Herbold CW
Rosebrock F 511 Sedlacek CJ
Milinovich G 1037 Horn M
Milinovich G 1037 Daims H
Milinovich G 1037 Berry D
Milinovich G 1037 Loy A
Milinovich G 1037 Wagner M
Milinovich G 1037 Eichorst SA
Milinovich G 1037 Mussmann M
Milinovich G 1037 Wasmund K
Milinovich G 1037 Herbold CW
Milinovich G 1037 Sedlacek CJ
Schlederer M 1268 Horn M
Schlederer M 1268 Daims H
Schlederer M 1268 Berry D
Schlederer M 1268 Loy A
Schlederer M 1268 Wagner M
Schlederer M 1268 Eichorst SA
Schlederer M 1268 Mussmann M
Schlederer M 1268 Wasmund K
Schlederer M 1268 Herbold CW
Schlederer M 1268 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Schleper C 405 Horn M
Schleper C 405 Daims H
Schleper C 405 Berry D
Schleper C 405 Loy A
Schleper C 405 Wagner M
Schleper C 405 Eichorst SA
Schleper C 405 Mussmann M
Schleper C 405 Wasmund K
Schleper C 405 Herbold CW
Schleper C 405 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Urich T 515 Horn M
Urich T 515 Daims H
Urich T 515 Berry D
Urich T 515 Loy A
Urich T 515 Wagner M
Urich T 515 Eichorst SA
Urich T 515 Mussmann M
Urich T 515 Wasmund K
Urich T 515 Herbold CW
Urich T 515 Sedlacek CJ
Kenner L 518 Horn M
Kenner L 518 Daims H
Kenner L 518 Berry D
Kenner L 518 Loy A
Kenner L 518 Wagner M
Kenner L 518 Eichorst SA
Kenner L 518 Mussmann M
Kenner L 518 Wasmund K
Kenner L 518 Herbold CW
Kenner L 518 Sedlacek CJ
Han X 1269 Horn M
Han X 1269 Daims H
Han X 1269 Berry D
Han X 1269 Loy A
Han X 1269 Wagner M
Han X 1269 Eichorst SA
Han X 1269 Mussmann M
Han X 1269 Wasmund K
Han X 1269 Herbold CW
Han X 1269 Sedlacek CJ
Decker T 287 Horn M
Decker T 287 Daims H
Decker T 287 Berry D
Decker T 287 Loy A
Decker T 287 Wagner M
Decker T 287 Eichorst SA
Decker T 287 Mussmann M
Decker T 287 Wasmund K
Decker T 287 Herbold CW
Decker T 287 Sedlacek CJ
Strobl B 517 Horn M
Strobl B 517 Daims H
Strobl B 517 Berry D
Strobl B 517 Loy A
Strobl B 517 Wagner M
Strobl B 517 Eichorst SA
Strobl B 517 Mussmann M
Strobl B 517 Wasmund K
Strobl B 517 Herbold CW
Strobl B 517 Sedlacek CJ
Müller M 516 Horn M
Müller M 516 Daims H
Müller M 516 Berry D
Müller M 516 Loy A
Müller M 516 Wagner M
Müller M 516 Eichorst SA
Müller M 516 Mussmann M
Müller M 516 Wasmund K
Müller M 516 Herbold CW
Müller M 516 Sedlacek CJ
Herbold CW 321 Horn M
Herbold CW 321 Daims H
Herbold CW 321 Berry D
Herbold CW 321 Loy A
Herbold CW 321 Wagner M
Herbold CW 321 Eichorst SA
Herbold CW 321 Mussmann M
Herbold CW 321 Wasmund K
Herbold CW 321 Herbold CW
Herbold CW 321 Sedlacek CJ
Pelikan C 322 Horn M
Pelikan C 322 Daims H
Pelikan C 322 Berry D
Pelikan C 322 Loy A
Pelikan C 322 Wagner M
Pelikan C 322 Eichorst SA
Pelikan C 322 Mussmann M
Pelikan C 322 Wasmund K
Pelikan C 322 Herbold CW
Pelikan C 322 Sedlacek CJ
Kuzyk O 323 Horn M
Kuzyk O 323 Daims H
Kuzyk O 323 Berry D
Kuzyk O 323 Loy A
Kuzyk O 323 Wagner M
Kuzyk O 323 Eichorst SA
Kuzyk O 323 Mussmann M
Kuzyk O 323 Wasmund K
Kuzyk O 323 Herbold CW
Kuzyk O 323 Sedlacek CJ
Hausmann B 324 Horn M
Hausmann B 324 Daims H
Hausmann B 324 Berry D
Hausmann B 324 Loy A
Hausmann B 324 Wagner M
Hausmann B 324 Eichorst SA
Hausmann B 324 Mussmann M
Hausmann B 324 Wasmund K
Hausmann B 324 Herbold CW
Hausmann B 324 Sedlacek CJ
Angel R 325 Horn M
Angel R 325 Daims H
Angel R 325 Berry D
Angel R 325 Loy A
Angel R 325 Wagner M
Angel R 325 Eichorst SA
Angel R 325 Mussmann M
Angel R 325 Wasmund K
Angel R 325 Herbold CW
Angel R 325 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Palatinszky M 281 Horn M
Palatinszky M 281 Daims H
Palatinszky M 281 Berry D
Palatinszky M 281 Loy A
Palatinszky M 281 Wagner M
Palatinszky M 281 Eichorst SA
Palatinszky M 281 Mussmann M
Palatinszky M 281 Wasmund K
Palatinszky M 281 Herbold CW
Palatinszky M 281 Sedlacek CJ
Herbold C 372 Horn M
Herbold C 372 Daims H
Herbold C 372 Berry D
Herbold C 372 Loy A
Herbold C 372 Wagner M
Herbold C 372 Eichorst SA
Herbold C 372 Mussmann M
Herbold C 372 Wasmund K
Herbold C 372 Herbold CW
Herbold C 372 Sedlacek CJ
Jehmlich N 373 Horn M
Jehmlich N 373 Daims H
Jehmlich N 373 Berry D
Jehmlich N 373 Loy A
Jehmlich N 373 Wagner M
Jehmlich N 373 Eichorst SA
Jehmlich N 373 Mussmann M
Jehmlich N 373 Wasmund K
Jehmlich N 373 Herbold CW
Jehmlich N 373 Sedlacek CJ
Pogoda M 374 Horn M
Pogoda M 374 Daims H
Pogoda M 374 Berry D
Pogoda M 374 Loy A
Pogoda M 374 Wagner M
Pogoda M 374 Eichorst SA
Pogoda M 374 Mussmann M
Pogoda M 374 Wasmund K
Pogoda M 374 Herbold CW
Pogoda M 374 Sedlacek CJ
Han P 375 Horn M
Han P 375 Daims H
Han P 375 Berry D
Han P 375 Loy A
Han P 375 Wagner M
Han P 375 Eichorst SA
Han P 375 Mussmann M
Han P 375 Wasmund K
Han P 375 Herbold CW
Han P 375 Sedlacek CJ
von Bergen M 376 Horn M
von Bergen M 376 Daims H
von Bergen M 376 Berry D
von Bergen M 376 Loy A
von Bergen M 376 Wagner M
von Bergen M 376 Eichorst SA
von Bergen M 376 Mussmann M
von Bergen M 376 Wasmund K
von Bergen M 376 Herbold CW
von Bergen M 376 Sedlacek CJ
Lagkouvardos I 377 Horn M
Lagkouvardos I 377 Daims H
Lagkouvardos I 377 Berry D
Lagkouvardos I 377 Loy A
Lagkouvardos I 377 Wagner M
Lagkouvardos I 377 Eichorst SA
Lagkouvardos I 377 Mussmann M
Lagkouvardos I 377 Wasmund K
Lagkouvardos I 377 Herbold CW
Lagkouvardos I 377 Sedlacek CJ
Karst SM 378 Horn M
Karst SM 378 Daims H
Karst SM 378 Berry D
Karst SM 378 Loy A
Karst SM 378 Wagner M
Karst SM 378 Eichorst SA
Karst SM 378 Mussmann M
Karst SM 378 Wasmund K
Karst SM 378 Herbold CW
Karst SM 378 Sedlacek CJ
Galushko A 379 Horn M
Galushko A 379 Daims H
Galushko A 379 Berry D
Galushko A 379 Loy A
Galushko A 379 Wagner M
Galushko A 379 Eichorst SA
Galushko A 379 Mussmann M
Galushko A 379 Wasmund K
Galushko A 379 Herbold CW
Galushko A 379 Sedlacek CJ
Koch H 380 Horn M
Koch H 380 Daims H
Koch H 380 Berry D
Koch H 380 Loy A
Koch H 380 Wagner M
Koch H 380 Eichorst SA
Koch H 380 Mussmann M
Koch H 380 Wasmund K
Koch H 380 Herbold CW
Koch H 380 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Mader E 276 Horn M
Mader E 276 Daims H
Mader E 276 Berry D
Mader E 276 Loy A
Mader E 276 Wagner M
Mader E 276 Eichorst SA
Mader E 276 Mussmann M
Mader E 276 Wasmund K
Mader E 276 Herbold CW
Mader E 276 Sedlacek CJ
Lee TK 277 Horn M
Lee TK 277 Daims H
Lee TK 277 Berry D
Lee TK 277 Loy A
Lee TK 277 Wagner M
Lee TK 277 Eichorst SA
Lee TK 277 Mussmann M
Lee TK 277 Wasmund K
Lee TK 277 Herbold CW
Lee TK 277 Sedlacek CJ
Woebken D 278 Horn M
Woebken D 278 Daims H
Woebken D 278 Berry D
Woebken D 278 Loy A
Woebken D 278 Wagner M
Woebken D 278 Eichorst SA
Woebken D 278 Mussmann M
Woebken D 278 Wasmund K
Woebken D 278 Herbold CW
Woebken D 278 Sedlacek CJ
Wang Y 279 Horn M
Wang Y 279 Daims H
Wang Y 279 Berry D
Wang Y 279 Loy A
Wang Y 279 Wagner M
Wang Y 279 Eichorst SA
Wang Y 279 Mussmann M
Wang Y 279 Wasmund K
Wang Y 279 Herbold CW
Wang Y 279 Sedlacek CJ
Zhu D 280 Horn M
Zhu D 280 Daims H
Zhu D 280 Berry D
Zhu D 280 Loy A
Zhu D 280 Wagner M
Zhu D 280 Eichorst SA
Zhu D 280 Mussmann M
Zhu D 280 Wasmund K
Zhu D 280 Herbold CW
Zhu D 280 Sedlacek CJ
Palatinszky M 281 Horn M
Palatinszky M 281 Daims H
Palatinszky M 281 Berry D
Palatinszky M 281 Loy A
Palatinszky M 281 Wagner M
Palatinszky M 281 Eichorst SA
Palatinszky M 281 Mussmann M
Palatinszky M 281 Wasmund K
Palatinszky M 281 Herbold CW
Palatinszky M 281 Sedlacek CJ
Schintlmeister A 282 Horn M
Schintlmeister A 282 Daims H
Schintlmeister A 282 Berry D
Schintlmeister A 282 Loy A
Schintlmeister A 282 Wagner M
Schintlmeister A 282 Eichorst SA
Schintlmeister A 282 Mussmann M
Schintlmeister A 282 Wasmund K
Schintlmeister A 282 Herbold CW
Schintlmeister A 282 Sedlacek CJ
Schmid MC 267 Horn M
Schmid MC 267 Daims H
Schmid MC 267 Berry D
Schmid MC 267 Loy A
Schmid MC 267 Wagner M
Schmid MC 267 Eichorst SA
Schmid MC 267 Mussmann M
Schmid MC 267 Wasmund K
Schmid MC 267 Herbold CW
Schmid MC 267 Sedlacek CJ
Hanson BT 283 Horn M
Hanson BT 283 Daims H
Hanson BT 283 Berry D
Hanson BT 283 Loy A
Hanson BT 283 Wagner M
Hanson BT 283 Eichorst SA
Hanson BT 283 Mussmann M
Hanson BT 283 Wasmund K
Hanson BT 283 Herbold CW
Hanson BT 283 Sedlacek CJ
Shterzer N 284 Horn M
Shterzer N 284 Daims H
Shterzer N 284 Berry D
Shterzer N 284 Loy A
Shterzer N 284 Wagner M
Shterzer N 284 Eichorst SA
Shterzer N 284 Mussmann M
Shterzer N 284 Wasmund K
Shterzer N 284 Herbold CW
Shterzer N 284 Sedlacek CJ
Mizrahi I 285 Horn M
Mizrahi I 285 Daims H
Mizrahi I 285 Berry D
Mizrahi I 285 Loy A
Mizrahi I 285 Wagner M
Mizrahi I 285 Eichorst SA
Mizrahi I 285 Mussmann M
Mizrahi I 285 Wasmund K
Mizrahi I 285 Herbold CW
Mizrahi I 285 Sedlacek CJ
Rauch I 286 Horn M
Rauch I 286 Daims H
Rauch I 286 Berry D
Rauch I 286 Loy A
Rauch I 286 Wagner M
Rauch I 286 Eichorst SA
Rauch I 286 Mussmann M
Rauch I 286 Wasmund K
Rauch I 286 Herbold CW
Rauch I 286 Sedlacek CJ
Decker T 287 Horn M
Decker T 287 Daims H
Decker T 287 Berry D
Decker T 287 Loy A
Decker T 287 Wagner M
Decker T 287 Eichorst SA
Decker T 287 Mussmann M
Decker T 287 Wasmund K
Decker T 287 Herbold CW
Decker T 287 Sedlacek CJ
Bocklitz T 288 Horn M
Bocklitz T 288 Daims H
Bocklitz T 288 Berry D
Bocklitz T 288 Loy A
Bocklitz T 288 Wagner M
Bocklitz T 288 Eichorst SA
Bocklitz T 288 Mussmann M
Bocklitz T 288 Wasmund K
Bocklitz T 288 Herbold CW
Bocklitz T 288 Sedlacek CJ
Popp J 289 Horn M
Popp J 289 Daims H
Popp J 289 Berry D
Popp J 289 Loy A
Popp J 289 Wagner M
Popp J 289 Eichorst SA
Popp J 289 Mussmann M
Popp J 289 Wasmund K
Popp J 289 Herbold CW
Popp J 289 Sedlacek CJ
Gibson CM 290 Horn M
Gibson CM 290 Daims H
Gibson CM 290 Berry D
Gibson CM 290 Loy A
Gibson CM 290 Wagner M
Gibson CM 290 Eichorst SA
Gibson CM 290 Mussmann M
Gibson CM 290 Wasmund K
Gibson CM 290 Herbold CW
Gibson CM 290 Sedlacek CJ
Fowler PW 291 Horn M
Fowler PW 291 Daims H
Fowler PW 291 Berry D
Fowler PW 291 Loy A
Fowler PW 291 Wagner M
Fowler PW 291 Eichorst SA
Fowler PW 291 Mussmann M
Fowler PW 291 Wasmund K
Fowler PW 291 Herbold CW
Fowler PW 291 Sedlacek CJ
Huang WE 292 Horn M
Huang WE 292 Daims H
Huang WE 292 Berry D
Huang WE 292 Loy A
Huang WE 292 Wagner M
Huang WE 292 Eichorst SA
Huang WE 292 Mussmann M
Huang WE 292 Wasmund K
Huang WE 292 Herbold CW
Huang WE 292 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Rauch I 286 Horn M
Rauch I 286 Daims H
Rauch I 286 Berry D
Rauch I 286 Loy A
Rauch I 286 Wagner M
Rauch I 286 Eichorst SA
Rauch I 286 Mussmann M
Rauch I 286 Wasmund K
Rauch I 286 Herbold CW
Rauch I 286 Sedlacek CJ
Hainzl E 510 Horn M
Hainzl E 510 Daims H
Hainzl E 510 Berry D
Hainzl E 510 Loy A
Hainzl E 510 Wagner M
Hainzl E 510 Eichorst SA
Hainzl E 510 Mussmann M
Hainzl E 510 Wasmund K
Hainzl E 510 Herbold CW
Hainzl E 510 Sedlacek CJ
Rosebrock F 511 Horn M
Rosebrock F 511 Daims H
Rosebrock F 511 Berry D
Rosebrock F 511 Loy A
Rosebrock F 511 Wagner M
Rosebrock F 511 Eichorst SA
Rosebrock F 511 Mussmann M
Rosebrock F 511 Wasmund K
Rosebrock F 511 Herbold CW
Rosebrock F 511 Sedlacek CJ
Heider S 512 Horn M
Heider S 512 Daims H
Heider S 512 Berry D
Heider S 512 Loy A
Heider S 512 Wagner M
Heider S 512 Eichorst SA
Heider S 512 Mussmann M
Heider S 512 Wasmund K
Heider S 512 Herbold CW
Heider S 512 Sedlacek CJ
Schwab C 513 Horn M
Schwab C 513 Daims H
Schwab C 513 Berry D
Schwab C 513 Loy A
Schwab C 513 Wagner M
Schwab C 513 Eichorst SA
Schwab C 513 Mussmann M
Schwab C 513 Wasmund K
Schwab C 513 Herbold CW
Schwab C 513 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Stoiber D 514 Horn M
Stoiber D 514 Daims H
Stoiber D 514 Berry D
Stoiber D 514 Loy A
Stoiber D 514 Wagner M
Stoiber D 514 Eichorst SA
Stoiber D 514 Mussmann M
Stoiber D 514 Wasmund K
Stoiber D 514 Herbold CW
Stoiber D 514 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Schleper C 405 Horn M
Schleper C 405 Daims H
Schleper C 405 Berry D
Schleper C 405 Loy A
Schleper C 405 Wagner M
Schleper C 405 Eichorst SA
Schleper C 405 Mussmann M
Schleper C 405 Wasmund K
Schleper C 405 Herbold CW
Schleper C 405 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Urich T 515 Horn M
Urich T 515 Daims H
Urich T 515 Berry D
Urich T 515 Loy A
Urich T 515 Wagner M
Urich T 515 Eichorst SA
Urich T 515 Mussmann M
Urich T 515 Wasmund K
Urich T 515 Herbold CW
Urich T 515 Sedlacek CJ
Müller M 516 Horn M
Müller M 516 Daims H
Müller M 516 Berry D
Müller M 516 Loy A
Müller M 516 Wagner M
Müller M 516 Eichorst SA
Müller M 516 Mussmann M
Müller M 516 Wasmund K
Müller M 516 Herbold CW
Müller M 516 Sedlacek CJ
Strobl B 517 Horn M
Strobl B 517 Daims H
Strobl B 517 Berry D
Strobl B 517 Loy A
Strobl B 517 Wagner M
Strobl B 517 Eichorst SA
Strobl B 517 Mussmann M
Strobl B 517 Wasmund K
Strobl B 517 Herbold CW
Strobl B 517 Sedlacek CJ
Kenner L 518 Horn M
Kenner L 518 Daims H
Kenner L 518 Berry D
Kenner L 518 Loy A
Kenner L 518 Wagner M
Kenner L 518 Eichorst SA
Kenner L 518 Mussmann M
Kenner L 518 Wasmund K
Kenner L 518 Herbold CW
Kenner L 518 Sedlacek CJ
Decker T 287 Horn M
Decker T 287 Daims H
Decker T 287 Berry D
Decker T 287 Loy A
Decker T 287 Wagner M
Decker T 287 Eichorst SA
Decker T 287 Mussmann M
Decker T 287 Wasmund K
Decker T 287 Herbold CW
Decker T 287 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Widder S 662 Horn M
Widder S 662 Daims H
Widder S 662 Berry D
Widder S 662 Loy A
Widder S 662 Wagner M
Widder S 662 Eichorst SA
Widder S 662 Mussmann M
Widder S 662 Wasmund K
Widder S 662 Herbold CW
Widder S 662 Sedlacek CJ
Daniel H 713 Horn M
Daniel H 713 Daims H
Daniel H 713 Berry D
Daniel H 713 Loy A
Daniel H 713 Wagner M
Daniel H 713 Eichorst SA
Daniel H 713 Mussmann M
Daniel H 713 Wasmund K
Daniel H 713 Herbold CW
Daniel H 713 Sedlacek CJ
Moghaddas Gholami A 714 Horn M
Moghaddas Gholami A 714 Daims H
Moghaddas Gholami A 714 Berry D
Moghaddas Gholami A 714 Loy A
Moghaddas Gholami A 714 Wagner M
Moghaddas Gholami A 714 Eichorst SA
Moghaddas Gholami A 714 Mussmann M
Moghaddas Gholami A 714 Wasmund K
Moghaddas Gholami A 714 Herbold CW
Moghaddas Gholami A 714 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Desmarchelier C 715 Horn M
Desmarchelier C 715 Daims H
Desmarchelier C 715 Berry D
Desmarchelier C 715 Loy A
Desmarchelier C 715 Wagner M
Desmarchelier C 715 Eichorst SA
Desmarchelier C 715 Mussmann M
Desmarchelier C 715 Wasmund K
Desmarchelier C 715 Herbold CW
Desmarchelier C 715 Sedlacek CJ
Hahne H 716 Horn M
Hahne H 716 Daims H
Hahne H 716 Berry D
Hahne H 716 Loy A
Hahne H 716 Wagner M
Hahne H 716 Eichorst SA
Hahne H 716 Mussmann M
Hahne H 716 Wasmund K
Hahne H 716 Herbold CW
Hahne H 716 Sedlacek CJ
Loh G 717 Horn M
Loh G 717 Daims H
Loh G 717 Berry D
Loh G 717 Loy A
Loh G 717 Wagner M
Loh G 717 Eichorst SA
Loh G 717 Mussmann M
Loh G 717 Wasmund K
Loh G 717 Herbold CW
Loh G 717 Sedlacek CJ
Mondot S 718 Horn M
Mondot S 718 Daims H
Mondot S 718 Berry D
Mondot S 718 Loy A
Mondot S 718 Wagner M
Mondot S 718 Eichorst SA
Mondot S 718 Mussmann M
Mondot S 718 Wasmund K
Mondot S 718 Herbold CW
Mondot S 718 Sedlacek CJ
Lepage P 719 Horn M
Lepage P 719 Daims H
Lepage P 719 Berry D
Lepage P 719 Loy A
Lepage P 719 Wagner M
Lepage P 719 Eichorst SA
Lepage P 719 Mussmann M
Lepage P 719 Wasmund K
Lepage P 719 Herbold CW
Lepage P 719 Sedlacek CJ
Rothballer M 720 Horn M
Rothballer M 720 Daims H
Rothballer M 720 Berry D
Rothballer M 720 Loy A
Rothballer M 720 Wagner M
Rothballer M 720 Eichorst SA
Rothballer M 720 Mussmann M
Rothballer M 720 Wasmund K
Rothballer M 720 Herbold CW
Rothballer M 720 Sedlacek CJ
Walker A 721 Horn M
Walker A 721 Daims H
Walker A 721 Berry D
Walker A 721 Loy A
Walker A 721 Wagner M
Walker A 721 Eichorst SA
Walker A 721 Mussmann M
Walker A 721 Wasmund K
Walker A 721 Herbold CW
Walker A 721 Sedlacek CJ
Böhm C 722 Horn M
Böhm C 722 Daims H
Böhm C 722 Berry D
Böhm C 722 Loy A
Böhm C 722 Wagner M
Böhm C 722 Eichorst SA
Böhm C 722 Mussmann M
Böhm C 722 Wasmund K
Böhm C 722 Herbold CW
Böhm C 722 Sedlacek CJ
Wenning M 723 Horn M
Wenning M 723 Daims H
Wenning M 723 Berry D
Wenning M 723 Loy A
Wenning M 723 Wagner M
Wenning M 723 Eichorst SA
Wenning M 723 Mussmann M
Wenning M 723 Wasmund K
Wenning M 723 Herbold CW
Wenning M 723 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Blaut M 724 Horn M
Blaut M 724 Daims H
Blaut M 724 Berry D
Blaut M 724 Loy A
Blaut M 724 Wagner M
Blaut M 724 Eichorst SA
Blaut M 724 Mussmann M
Blaut M 724 Wasmund K
Blaut M 724 Herbold CW
Blaut M 724 Sedlacek CJ
Schmitt-Kopplin P 725 Horn M
Schmitt-Kopplin P 725 Daims H
Schmitt-Kopplin P 725 Berry D
Schmitt-Kopplin P 725 Loy A
Schmitt-Kopplin P 725 Wagner M
Schmitt-Kopplin P 725 Eichorst SA
Schmitt-Kopplin P 725 Mussmann M
Schmitt-Kopplin P 725 Wasmund K
Schmitt-Kopplin P 725 Herbold CW
Schmitt-Kopplin P 725 Sedlacek CJ
Kuster B 726 Horn M
Kuster B 726 Daims H
Kuster B 726 Berry D
Kuster B 726 Loy A
Kuster B 726 Wagner M
Kuster B 726 Eichorst SA
Kuster B 726 Mussmann M
Kuster B 726 Wasmund K
Kuster B 726 Herbold CW
Kuster B 726 Sedlacek CJ
Haller D 727 Horn M
Haller D 727 Daims H
Haller D 727 Berry D
Haller D 727 Loy A
Haller D 727 Wagner M
Haller D 727 Eichorst SA
Haller D 727 Mussmann M
Haller D 727 Wasmund K
Haller D 727 Herbold CW
Haller D 727 Sedlacek CJ
Clavel T 728 Horn M
Clavel T 728 Daims H
Clavel T 728 Berry D
Clavel T 728 Loy A
Clavel T 728 Wagner M
Clavel T 728 Eichorst SA
Clavel T 728 Mussmann M
Clavel T 728 Wasmund K
Clavel T 728 Herbold CW
Clavel T 728 Sedlacek CJ
Oneisis-Barry K 551 Horn M
Oneisis-Barry K 551 Daims H
Oneisis-Barry K 551 Berry D
Oneisis-Barry K 551 Loy A
Oneisis-Barry K 551 Wagner M
Oneisis-Barry K 551 Eichorst SA
Oneisis-Barry K 551 Mussmann M
Oneisis-Barry K 551 Wasmund K
Oneisis-Barry K 551 Herbold CW
Oneisis-Barry K 551 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Proscher J 552 Horn M
Proscher J 552 Daims H
Proscher J 552 Berry D
Proscher J 552 Loy A
Proscher J 552 Wagner M
Proscher J 552 Eichorst SA
Proscher J 552 Mussmann M
Proscher J 552 Wasmund K
Proscher J 552 Herbold CW
Proscher J 552 Sedlacek CJ
Sivakumar IKA 553 Horn M
Sivakumar IKA 553 Daims H
Sivakumar IKA 553 Berry D
Sivakumar IKA 553 Loy A
Sivakumar IKA 553 Wagner M
Sivakumar IKA 553 Eichorst SA
Sivakumar IKA 553 Mussmann M
Sivakumar IKA 553 Wasmund K
Sivakumar IKA 553 Herbold CW
Sivakumar IKA 553 Sedlacek CJ
Bouwer E 554 Horn M
Bouwer E 554 Daims H
Bouwer E 554 Berry D
Bouwer E 554 Loy A
Bouwer E 554 Wagner M
Bouwer E 554 Eichorst SA
Bouwer E 554 Mussmann M
Bouwer E 554 Wasmund K
Bouwer E 554 Herbold CW
Bouwer E 554 Sedlacek CJ
Schwab C 513 Horn M
Schwab C 513 Daims H
Schwab C 513 Berry D
Schwab C 513 Loy A
Schwab C 513 Wagner M
Schwab C 513 Eichorst SA
Schwab C 513 Mussmann M
Schwab C 513 Wasmund K
Schwab C 513 Herbold CW
Schwab C 513 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Rauch I 286 Horn M
Rauch I 286 Daims H
Rauch I 286 Berry D
Rauch I 286 Loy A
Rauch I 286 Wagner M
Rauch I 286 Eichorst SA
Rauch I 286 Mussmann M
Rauch I 286 Wasmund K
Rauch I 286 Herbold CW
Rauch I 286 Sedlacek CJ
Rennisch I 711 Horn M
Rennisch I 711 Daims H
Rennisch I 711 Berry D
Rennisch I 711 Loy A
Rennisch I 711 Wagner M
Rennisch I 711 Eichorst SA
Rennisch I 711 Mussmann M
Rennisch I 711 Wasmund K
Rennisch I 711 Herbold CW
Rennisch I 711 Sedlacek CJ
Ramesmayer J 712 Horn M
Ramesmayer J 712 Daims H
Ramesmayer J 712 Berry D
Ramesmayer J 712 Loy A
Ramesmayer J 712 Wagner M
Ramesmayer J 712 Eichorst SA
Ramesmayer J 712 Mussmann M
Ramesmayer J 712 Wasmund K
Ramesmayer J 712 Herbold CW
Ramesmayer J 712 Sedlacek CJ
Hainzl E 510 Horn M
Hainzl E 510 Daims H
Hainzl E 510 Berry D
Hainzl E 510 Loy A
Hainzl E 510 Wagner M
Hainzl E 510 Eichorst SA
Hainzl E 510 Mussmann M
Hainzl E 510 Wasmund K
Hainzl E 510 Herbold CW
Hainzl E 510 Sedlacek CJ
Heider S 512 Horn M
Heider S 512 Daims H
Heider S 512 Berry D
Heider S 512 Loy A
Heider S 512 Wagner M
Heider S 512 Eichorst SA
Heider S 512 Mussmann M
Heider S 512 Wasmund K
Heider S 512 Herbold CW
Heider S 512 Sedlacek CJ
Decker T 287 Horn M
Decker T 287 Daims H
Decker T 287 Berry D
Decker T 287 Loy A
Decker T 287 Wagner M
Decker T 287 Eichorst SA
Decker T 287 Mussmann M
Decker T 287 Wasmund K
Decker T 287 Herbold CW
Decker T 287 Sedlacek CJ
Kenner L 518 Horn M
Kenner L 518 Daims H
Kenner L 518 Berry D
Kenner L 518 Loy A
Kenner L 518 Wagner M
Kenner L 518 Eichorst SA
Kenner L 518 Mussmann M
Kenner L 518 Wasmund K
Kenner L 518 Herbold CW
Kenner L 518 Sedlacek CJ
Müller M 516 Horn M
Müller M 516 Daims H
Müller M 516 Berry D
Müller M 516 Loy A
Müller M 516 Wagner M
Müller M 516 Eichorst SA
Müller M 516 Mussmann M
Müller M 516 Wasmund K
Müller M 516 Herbold CW
Müller M 516 Sedlacek CJ
Strobl B 517 Horn M
Strobl B 517 Daims H
Strobl B 517 Berry D
Strobl B 517 Loy A
Strobl B 517 Wagner M
Strobl B 517 Eichorst SA
Strobl B 517 Mussmann M
Strobl B 517 Wasmund K
Strobl B 517 Herbold CW
Strobl B 517 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Schleper C 405 Horn M
Schleper C 405 Daims H
Schleper C 405 Berry D
Schleper C 405 Loy A
Schleper C 405 Wagner M
Schleper C 405 Eichorst SA
Schleper C 405 Mussmann M
Schleper C 405 Wasmund K
Schleper C 405 Herbold CW
Schleper C 405 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Urich T 515 Horn M
Urich T 515 Daims H
Urich T 515 Berry D
Urich T 515 Loy A
Urich T 515 Wagner M
Urich T 515 Eichorst SA
Urich T 515 Mussmann M
Urich T 515 Wasmund K
Urich T 515 Herbold CW
Urich T 515 Sedlacek CJ
Maier I 575 Horn M
Maier I 575 Daims H
Maier I 575 Berry D
Maier I 575 Loy A
Maier I 575 Wagner M
Maier I 575 Eichorst SA
Maier I 575 Mussmann M
Maier I 575 Wasmund K
Maier I 575 Herbold CW
Maier I 575 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Schiesl R 576 Horn M
Schiesl R 576 Daims H
Schiesl R 576 Berry D
Schiesl R 576 Loy A
Schiesl R 576 Wagner M
Schiesl R 576 Eichorst SA
Schiesl R 576 Mussmann M
Schiesl R 576 Wasmund K
Schiesl R 576 Herbold CW
Schiesl R 576 Sedlacek CJ
Müller A 558 Horn M
Müller A 558 Daims H
Müller A 558 Berry D
Müller A 558 Loy A
Müller A 558 Wagner M
Müller A 558 Eichorst SA
Müller A 558 Mussmann M
Müller A 558 Wasmund K
Müller A 558 Herbold CW
Müller A 558 Sedlacek CJ
de Rezende JR 559 Horn M
de Rezende JR 559 Daims H
de Rezende JR 559 Berry D
de Rezende JR 559 Loy A
de Rezende JR 559 Wagner M
de Rezende JR 559 Eichorst SA
de Rezende JR 559 Mussmann M
de Rezende JR 559 Wasmund K
de Rezende JR 559 Herbold CW
de Rezende JR 559 Sedlacek CJ
Hubert C 560 Horn M
Hubert C 560 Daims H
Hubert C 560 Berry D
Hubert C 560 Loy A
Hubert C 560 Wagner M
Hubert C 560 Eichorst SA
Hubert C 560 Mussmann M
Hubert C 560 Wasmund K
Hubert C 560 Herbold CW
Hubert C 560 Sedlacek CJ
Kjeldsen KU 359 Horn M
Kjeldsen KU 359 Daims H
Kjeldsen KU 359 Berry D
Kjeldsen KU 359 Loy A
Kjeldsen KU 359 Wagner M
Kjeldsen KU 359 Eichorst SA
Kjeldsen KU 359 Mussmann M
Kjeldsen KU 359 Wasmund K
Kjeldsen KU 359 Herbold CW
Kjeldsen KU 359 Sedlacek CJ
Lagkouvardos I 377 Horn M
Lagkouvardos I 377 Daims H
Lagkouvardos I 377 Berry D
Lagkouvardos I 377 Loy A
Lagkouvardos I 377 Wagner M
Lagkouvardos I 377 Eichorst SA
Lagkouvardos I 377 Mussmann M
Lagkouvardos I 377 Wasmund K
Lagkouvardos I 377 Herbold CW
Lagkouvardos I 377 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Jørgensen BB 362 Horn M
Jørgensen BB 362 Daims H
Jørgensen BB 362 Berry D
Jørgensen BB 362 Loy A
Jørgensen BB 362 Wagner M
Jørgensen BB 362 Eichorst SA
Jørgensen BB 362 Mussmann M
Jørgensen BB 362 Wasmund K
Jørgensen BB 362 Herbold CW
Jørgensen BB 362 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Gutierrez T 642 Horn M
Gutierrez T 642 Daims H
Gutierrez T 642 Berry D
Gutierrez T 642 Loy A
Gutierrez T 642 Wagner M
Gutierrez T 642 Eichorst SA
Gutierrez T 642 Mussmann M
Gutierrez T 642 Wasmund K
Gutierrez T 642 Herbold CW
Gutierrez T 642 Sedlacek CJ
Rhodes G 643 Horn M
Rhodes G 643 Daims H
Rhodes G 643 Berry D
Rhodes G 643 Loy A
Rhodes G 643 Wagner M
Rhodes G 643 Eichorst SA
Rhodes G 643 Mussmann M
Rhodes G 643 Wasmund K
Rhodes G 643 Herbold CW
Rhodes G 643 Sedlacek CJ
Mishamandani S 644 Horn M
Mishamandani S 644 Daims H
Mishamandani S 644 Berry D
Mishamandani S 644 Loy A
Mishamandani S 644 Wagner M
Mishamandani S 644 Eichorst SA
Mishamandani S 644 Mussmann M
Mishamandani S 644 Wasmund K
Mishamandani S 644 Herbold CW
Mishamandani S 644 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Whitman WB 628 Horn M
Whitman WB 628 Daims H
Whitman WB 628 Berry D
Whitman WB 628 Loy A
Whitman WB 628 Wagner M
Whitman WB 628 Eichorst SA
Whitman WB 628 Mussmann M
Whitman WB 628 Wasmund K
Whitman WB 628 Herbold CW
Whitman WB 628 Sedlacek CJ
Nichols PD 645 Horn M
Nichols PD 645 Daims H
Nichols PD 645 Berry D
Nichols PD 645 Loy A
Nichols PD 645 Wagner M
Nichols PD 645 Eichorst SA
Nichols PD 645 Mussmann M
Nichols PD 645 Wasmund K
Nichols PD 645 Herbold CW
Nichols PD 645 Sedlacek CJ
Semple KT 646 Horn M
Semple KT 646 Daims H
Semple KT 646 Berry D
Semple KT 646 Loy A
Semple KT 646 Wagner M
Semple KT 646 Eichorst SA
Semple KT 646 Mussmann M
Semple KT 646 Wasmund K
Semple KT 646 Herbold CW
Semple KT 646 Sedlacek CJ
Aitken MD 647 Horn M
Aitken MD 647 Daims H
Aitken MD 647 Berry D
Aitken MD 647 Loy A
Aitken MD 647 Wagner M
Aitken MD 647 Eichorst SA
Aitken MD 647 Mussmann M
Aitken MD 647 Wasmund K
Aitken MD 647 Herbold CW
Aitken MD 647 Sedlacek CJ
Pester M 303 Horn M
Pester M 303 Daims H
Pester M 303 Berry D
Pester M 303 Loy A
Pester M 303 Wagner M
Pester M 303 Eichorst SA
Pester M 303 Mussmann M
Pester M 303 Wasmund K
Pester M 303 Herbold CW
Pester M 303 Sedlacek CJ
Maixner F 544 Horn M
Maixner F 544 Daims H
Maixner F 544 Berry D
Maixner F 544 Loy A
Maixner F 544 Wagner M
Maixner F 544 Eichorst SA
Maixner F 544 Mussmann M
Maixner F 544 Wasmund K
Maixner F 544 Herbold CW
Maixner F 544 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Rattei T 307 Horn M
Rattei T 307 Daims H
Rattei T 307 Berry D
Rattei T 307 Loy A
Rattei T 307 Wagner M
Rattei T 307 Eichorst SA
Rattei T 307 Mussmann M
Rattei T 307 Wasmund K
Rattei T 307 Herbold CW
Rattei T 307 Sedlacek CJ
Koch H 380 Horn M
Koch H 380 Daims H
Koch H 380 Berry D
Koch H 380 Loy A
Koch H 380 Wagner M
Koch H 380 Eichorst SA
Koch H 380 Mussmann M
Koch H 380 Wasmund K
Koch H 380 Herbold CW
Koch H 380 Sedlacek CJ
Lücker S 336 Horn M
Lücker S 336 Daims H
Lücker S 336 Berry D
Lücker S 336 Loy A
Lücker S 336 Wagner M
Lücker S 336 Eichorst SA
Lücker S 336 Mussmann M
Lücker S 336 Wasmund K
Lücker S 336 Herbold CW
Lücker S 336 Sedlacek CJ
Nowka B 363 Horn M
Nowka B 363 Daims H
Nowka B 363 Berry D
Nowka B 363 Loy A
Nowka B 363 Wagner M
Nowka B 363 Eichorst SA
Nowka B 363 Mussmann M
Nowka B 363 Wasmund K
Nowka B 363 Herbold CW
Nowka B 363 Sedlacek CJ
Richter A 545 Horn M
Richter A 545 Daims H
Richter A 545 Berry D
Richter A 545 Loy A
Richter A 545 Wagner M
Richter A 545 Eichorst SA
Richter A 545 Mussmann M
Richter A 545 Wasmund K
Richter A 545 Herbold CW
Richter A 545 Sedlacek CJ
Spieck E 338 Horn M
Spieck E 338 Daims H
Spieck E 338 Berry D
Spieck E 338 Loy A
Spieck E 338 Wagner M
Spieck E 338 Eichorst SA
Spieck E 338 Mussmann M
Spieck E 338 Wasmund K
Spieck E 338 Herbold CW
Spieck E 338 Sedlacek CJ
Lebedeva E 546 Horn M
Lebedeva E 546 Daims H
Lebedeva E 546 Berry D
Lebedeva E 546 Loy A
Lebedeva E 546 Wagner M
Lebedeva E 546 Eichorst SA
Lebedeva E 546 Mussmann M
Lebedeva E 546 Wasmund K
Lebedeva E 546 Herbold CW
Lebedeva E 546 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Daims H 308 Horn M
Daims H 308 Daims H
Daims H 308 Berry D
Daims H 308 Loy A
Daims H 308 Wagner M
Daims H 308 Eichorst SA
Daims H 308 Mussmann M
Daims H 308 Wasmund K
Daims H 308 Herbold CW
Daims H 308 Sedlacek CJ
Gutierrez T 642 Horn M
Gutierrez T 642 Daims H
Gutierrez T 642 Berry D
Gutierrez T 642 Loy A
Gutierrez T 642 Wagner M
Gutierrez T 642 Eichorst SA
Gutierrez T 642 Mussmann M
Gutierrez T 642 Wasmund K
Gutierrez T 642 Herbold CW
Gutierrez T 642 Sedlacek CJ
Rhodes G 643 Horn M
Rhodes G 643 Daims H
Rhodes G 643 Berry D
Rhodes G 643 Loy A
Rhodes G 643 Wagner M
Rhodes G 643 Eichorst SA
Rhodes G 643 Mussmann M
Rhodes G 643 Wasmund K
Rhodes G 643 Herbold CW
Rhodes G 643 Sedlacek CJ
Mishamandani S 644 Horn M
Mishamandani S 644 Daims H
Mishamandani S 644 Berry D
Mishamandani S 644 Loy A
Mishamandani S 644 Wagner M
Mishamandani S 644 Eichorst SA
Mishamandani S 644 Mussmann M
Mishamandani S 644 Wasmund K
Mishamandani S 644 Herbold CW
Mishamandani S 644 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Whitman WB 628 Horn M
Whitman WB 628 Daims H
Whitman WB 628 Berry D
Whitman WB 628 Loy A
Whitman WB 628 Wagner M
Whitman WB 628 Eichorst SA
Whitman WB 628 Mussmann M
Whitman WB 628 Wasmund K
Whitman WB 628 Herbold CW
Whitman WB 628 Sedlacek CJ
Nichols PD 645 Horn M
Nichols PD 645 Daims H
Nichols PD 645 Berry D
Nichols PD 645 Loy A
Nichols PD 645 Wagner M
Nichols PD 645 Eichorst SA
Nichols PD 645 Mussmann M
Nichols PD 645 Wasmund K
Nichols PD 645 Herbold CW
Nichols PD 645 Sedlacek CJ
Semple KT 646 Horn M
Semple KT 646 Daims H
Semple KT 646 Berry D
Semple KT 646 Loy A
Semple KT 646 Wagner M
Semple KT 646 Eichorst SA
Semple KT 646 Mussmann M
Semple KT 646 Wasmund K
Semple KT 646 Herbold CW
Semple KT 646 Sedlacek CJ
Aitken MD 647 Horn M
Aitken MD 647 Daims H
Aitken MD 647 Berry D
Aitken MD 647 Loy A
Aitken MD 647 Wagner M
Aitken MD 647 Eichorst SA
Aitken MD 647 Mussmann M
Aitken MD 647 Wasmund K
Aitken MD 647 Herbold CW
Aitken MD 647 Sedlacek CJ
Angelberger S 806 Horn M
Angelberger S 806 Daims H
Angelberger S 806 Berry D
Angelberger S 806 Loy A
Angelberger S 806 Wagner M
Angelberger S 806 Eichorst SA
Angelberger S 806 Mussmann M
Angelberger S 806 Wasmund K
Angelberger S 806 Herbold CW
Angelberger S 806 Sedlacek CJ
Reinisch W 805 Horn M
Reinisch W 805 Daims H
Reinisch W 805 Berry D
Reinisch W 805 Loy A
Reinisch W 805 Wagner M
Reinisch W 805 Eichorst SA
Reinisch W 805 Mussmann M
Reinisch W 805 Wasmund K
Reinisch W 805 Herbold CW
Reinisch W 805 Sedlacek CJ
Makristathis A 807 Horn M
Makristathis A 807 Daims H
Makristathis A 807 Berry D
Makristathis A 807 Loy A
Makristathis A 807 Wagner M
Makristathis A 807 Eichorst SA
Makristathis A 807 Mussmann M
Makristathis A 807 Wasmund K
Makristathis A 807 Herbold CW
Makristathis A 807 Sedlacek CJ
Lichtenberger C 808 Horn M
Lichtenberger C 808 Daims H
Lichtenberger C 808 Berry D
Lichtenberger C 808 Loy A
Lichtenberger C 808 Wagner M
Lichtenberger C 808 Eichorst SA
Lichtenberger C 808 Mussmann M
Lichtenberger C 808 Wasmund K
Lichtenberger C 808 Herbold CW
Lichtenberger C 808 Sedlacek CJ
Dejaco C 809 Horn M
Dejaco C 809 Daims H
Dejaco C 809 Berry D
Dejaco C 809 Loy A
Dejaco C 809 Wagner M
Dejaco C 809 Eichorst SA
Dejaco C 809 Mussmann M
Dejaco C 809 Wasmund K
Dejaco C 809 Herbold CW
Dejaco C 809 Sedlacek CJ
Papay P 810 Horn M
Papay P 810 Daims H
Papay P 810 Berry D
Papay P 810 Loy A
Papay P 810 Wagner M
Papay P 810 Eichorst SA
Papay P 810 Mussmann M
Papay P 810 Wasmund K
Papay P 810 Herbold CW
Papay P 810 Sedlacek CJ
Novacek G 811 Horn M
Novacek G 811 Daims H
Novacek G 811 Berry D
Novacek G 811 Loy A
Novacek G 811 Wagner M
Novacek G 811 Eichorst SA
Novacek G 811 Mussmann M
Novacek G 811 Wasmund K
Novacek G 811 Herbold CW
Novacek G 811 Sedlacek CJ
Trauner M 812 Horn M
Trauner M 812 Daims H
Trauner M 812 Berry D
Trauner M 812 Loy A
Trauner M 812 Wagner M
Trauner M 812 Eichorst SA
Trauner M 812 Mussmann M
Trauner M 812 Wasmund K
Trauner M 812 Herbold CW
Trauner M 812 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Yamamoto ML 856 Horn M
Yamamoto ML 856 Daims H
Yamamoto ML 856 Berry D
Yamamoto ML 856 Loy A
Yamamoto ML 856 Wagner M
Yamamoto ML 856 Eichorst SA
Yamamoto ML 856 Mussmann M
Yamamoto ML 856 Wasmund K
Yamamoto ML 856 Herbold CW
Yamamoto ML 856 Sedlacek CJ
Maier I 575 Horn M
Maier I 575 Daims H
Maier I 575 Berry D
Maier I 575 Loy A
Maier I 575 Wagner M
Maier I 575 Eichorst SA
Maier I 575 Mussmann M
Maier I 575 Wasmund K
Maier I 575 Herbold CW
Maier I 575 Sedlacek CJ
Dang AT 857 Horn M
Dang AT 857 Daims H
Dang AT 857 Berry D
Dang AT 857 Loy A
Dang AT 857 Wagner M
Dang AT 857 Eichorst SA
Dang AT 857 Mussmann M
Dang AT 857 Wasmund K
Dang AT 857 Herbold CW
Dang AT 857 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Liu J 858 Horn M
Liu J 858 Daims H
Liu J 858 Berry D
Liu J 858 Loy A
Liu J 858 Wagner M
Liu J 858 Eichorst SA
Liu J 858 Mussmann M
Liu J 858 Wasmund K
Liu J 858 Herbold CW
Liu J 858 Sedlacek CJ
Ruegger PM 859 Horn M
Ruegger PM 859 Daims H
Ruegger PM 859 Berry D
Ruegger PM 859 Loy A
Ruegger PM 859 Wagner M
Ruegger PM 859 Eichorst SA
Ruegger PM 859 Mussmann M
Ruegger PM 859 Wasmund K
Ruegger PM 859 Herbold CW
Ruegger PM 859 Sedlacek CJ
Yang J 860 Horn M
Yang J 860 Daims H
Yang J 860 Berry D
Yang J 860 Loy A
Yang J 860 Wagner M
Yang J 860 Eichorst SA
Yang J 860 Mussmann M
Yang J 860 Wasmund K
Yang J 860 Herbold CW
Yang J 860 Sedlacek CJ
Soto PA 861 Horn M
Soto PA 861 Daims H
Soto PA 861 Berry D
Soto PA 861 Loy A
Soto PA 861 Wagner M
Soto PA 861 Eichorst SA
Soto PA 861 Mussmann M
Soto PA 861 Wasmund K
Soto PA 861 Herbold CW
Soto PA 861 Sedlacek CJ
Presley LL 862 Horn M
Presley LL 862 Daims H
Presley LL 862 Berry D
Presley LL 862 Loy A
Presley LL 862 Wagner M
Presley LL 862 Eichorst SA
Presley LL 862 Mussmann M
Presley LL 862 Wasmund K
Presley LL 862 Herbold CW
Presley LL 862 Sedlacek CJ
Reliene R 863 Horn M
Reliene R 863 Daims H
Reliene R 863 Berry D
Reliene R 863 Loy A
Reliene R 863 Wagner M
Reliene R 863 Eichorst SA
Reliene R 863 Mussmann M
Reliene R 863 Wasmund K
Reliene R 863 Herbold CW
Reliene R 863 Sedlacek CJ
Westbrook AM 864 Horn M
Westbrook AM 864 Daims H
Westbrook AM 864 Berry D
Westbrook AM 864 Loy A
Westbrook AM 864 Wagner M
Westbrook AM 864 Eichorst SA
Westbrook AM 864 Mussmann M
Westbrook AM 864 Wasmund K
Westbrook AM 864 Herbold CW
Westbrook AM 864 Sedlacek CJ
Wei B 865 Horn M
Wei B 865 Daims H
Wei B 865 Berry D
Wei B 865 Loy A
Wei B 865 Wagner M
Wei B 865 Eichorst SA
Wei B 865 Mussmann M
Wei B 865 Wasmund K
Wei B 865 Herbold CW
Wei B 865 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Chang C 866 Horn M
Chang C 866 Daims H
Chang C 866 Berry D
Chang C 866 Loy A
Chang C 866 Wagner M
Chang C 866 Eichorst SA
Chang C 866 Mussmann M
Chang C 866 Wasmund K
Chang C 866 Herbold CW
Chang C 866 Sedlacek CJ
Braun J 867 Horn M
Braun J 867 Daims H
Braun J 867 Berry D
Braun J 867 Loy A
Braun J 867 Wagner M
Braun J 867 Eichorst SA
Braun J 867 Mussmann M
Braun J 867 Wasmund K
Braun J 867 Herbold CW
Braun J 867 Sedlacek CJ
Borneman J 868 Horn M
Borneman J 868 Daims H
Borneman J 868 Berry D
Borneman J 868 Loy A
Borneman J 868 Wagner M
Borneman J 868 Eichorst SA
Borneman J 868 Mussmann M
Borneman J 868 Wasmund K
Borneman J 868 Herbold CW
Borneman J 868 Sedlacek CJ
Schiestl RH 869 Horn M
Schiestl RH 869 Daims H
Schiestl RH 869 Berry D
Schiestl RH 869 Loy A
Schiestl RH 869 Wagner M
Schiestl RH 869 Eichorst SA
Schiestl RH 869 Mussmann M
Schiestl RH 869 Wasmund K
Schiestl RH 869 Herbold CW
Schiestl RH 869 Sedlacek CJ
Gutierrez T 642 Horn M
Gutierrez T 642 Daims H
Gutierrez T 642 Berry D
Gutierrez T 642 Loy A
Gutierrez T 642 Wagner M
Gutierrez T 642 Eichorst SA
Gutierrez T 642 Mussmann M
Gutierrez T 642 Wasmund K
Gutierrez T 642 Herbold CW
Gutierrez T 642 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Yang T 783 Horn M
Yang T 783 Daims H
Yang T 783 Berry D
Yang T 783 Loy A
Yang T 783 Wagner M
Yang T 783 Eichorst SA
Yang T 783 Mussmann M
Yang T 783 Wasmund K
Yang T 783 Herbold CW
Yang T 783 Sedlacek CJ
Mishamandani S 644 Horn M
Mishamandani S 644 Daims H
Mishamandani S 644 Berry D
Mishamandani S 644 Loy A
Mishamandani S 644 Wagner M
Mishamandani S 644 Eichorst SA
Mishamandani S 644 Mussmann M
Mishamandani S 644 Wasmund K
Mishamandani S 644 Herbold CW
Mishamandani S 644 Sedlacek CJ
McKay L 784 Horn M
McKay L 784 Daims H
McKay L 784 Berry D
McKay L 784 Loy A
McKay L 784 Wagner M
McKay L 784 Eichorst SA
McKay L 784 Mussmann M
McKay L 784 Wasmund K
McKay L 784 Herbold CW
McKay L 784 Sedlacek CJ
Teske A 785 Horn M
Teske A 785 Daims H
Teske A 785 Berry D
Teske A 785 Loy A
Teske A 785 Wagner M
Teske A 785 Eichorst SA
Teske A 785 Mussmann M
Teske A 785 Wasmund K
Teske A 785 Herbold CW
Teske A 785 Sedlacek CJ
Aitken MD 647 Horn M
Aitken MD 647 Daims H
Aitken MD 647 Berry D
Aitken MD 647 Loy A
Aitken MD 647 Wagner M
Aitken MD 647 Eichorst SA
Aitken MD 647 Mussmann M
Aitken MD 647 Wasmund K
Aitken MD 647 Herbold CW
Aitken MD 647 Sedlacek CJ
Stecher B 801 Horn M
Stecher B 801 Daims H
Stecher B 801 Berry D
Stecher B 801 Loy A
Stecher B 801 Wagner M
Stecher B 801 Eichorst SA
Stecher B 801 Mussmann M
Stecher B 801 Wasmund K
Stecher B 801 Herbold CW
Stecher B 801 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Stecher B 801 Horn M
Stecher B 801 Daims H
Stecher B 801 Berry D
Stecher B 801 Loy A
Stecher B 801 Wagner M
Stecher B 801 Eichorst SA
Stecher B 801 Mussmann M
Stecher B 801 Wasmund K
Stecher B 801 Herbold CW
Stecher B 801 Sedlacek CJ
Schintlmeister A 282 Horn M
Schintlmeister A 282 Daims H
Schintlmeister A 282 Berry D
Schintlmeister A 282 Loy A
Schintlmeister A 282 Wagner M
Schintlmeister A 282 Eichorst SA
Schintlmeister A 282 Mussmann M
Schintlmeister A 282 Wasmund K
Schintlmeister A 282 Herbold CW
Schintlmeister A 282 Sedlacek CJ
Reichert J 802 Horn M
Reichert J 802 Daims H
Reichert J 802 Berry D
Reichert J 802 Loy A
Reichert J 802 Wagner M
Reichert J 802 Eichorst SA
Reichert J 802 Mussmann M
Reichert J 802 Wasmund K
Reichert J 802 Herbold CW
Reichert J 802 Sedlacek CJ
Brugiroux S 803 Horn M
Brugiroux S 803 Daims H
Brugiroux S 803 Berry D
Brugiroux S 803 Loy A
Brugiroux S 803 Wagner M
Brugiroux S 803 Eichorst SA
Brugiroux S 803 Mussmann M
Brugiroux S 803 Wasmund K
Brugiroux S 803 Herbold CW
Brugiroux S 803 Sedlacek CJ
Wildd B 804 Horn M
Wildd B 804 Daims H
Wildd B 804 Berry D
Wildd B 804 Loy A
Wildd B 804 Wagner M
Wildd B 804 Eichorst SA
Wildd B 804 Mussmann M
Wildd B 804 Wasmund K
Wildd B 804 Herbold CW
Wildd B 804 Sedlacek CJ
Wanek W 787 Horn M
Wanek W 787 Daims H
Wanek W 787 Berry D
Wanek W 787 Loy A
Wanek W 787 Wagner M
Wanek W 787 Eichorst SA
Wanek W 787 Mussmann M
Wanek W 787 Wasmund K
Wanek W 787 Herbold CW
Wanek W 787 Sedlacek CJ
Richter A 545 Horn M
Richter A 545 Daims H
Richter A 545 Berry D
Richter A 545 Loy A
Richter A 545 Wagner M
Richter A 545 Eichorst SA
Richter A 545 Mussmann M
Richter A 545 Wasmund K
Richter A 545 Herbold CW
Richter A 545 Sedlacek CJ
Rauch I 286 Horn M
Rauch I 286 Daims H
Rauch I 286 Berry D
Rauch I 286 Loy A
Rauch I 286 Wagner M
Rauch I 286 Eichorst SA
Rauch I 286 Mussmann M
Rauch I 286 Wasmund K
Rauch I 286 Herbold CW
Rauch I 286 Sedlacek CJ
Decker T 287 Horn M
Decker T 287 Daims H
Decker T 287 Berry D
Decker T 287 Loy A
Decker T 287 Wagner M
Decker T 287 Eichorst SA
Decker T 287 Mussmann M
Decker T 287 Wasmund K
Decker T 287 Herbold CW
Decker T 287 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Reinisch W 805 Horn M
Reinisch W 805 Daims H
Reinisch W 805 Berry D
Reinisch W 805 Loy A
Reinisch W 805 Wagner M
Reinisch W 805 Eichorst SA
Reinisch W 805 Mussmann M
Reinisch W 805 Wasmund K
Reinisch W 805 Herbold CW
Reinisch W 805 Sedlacek CJ
Gutierrez T 642 Horn M
Gutierrez T 642 Daims H
Gutierrez T 642 Berry D
Gutierrez T 642 Loy A
Gutierrez T 642 Wagner M
Gutierrez T 642 Eichorst SA
Gutierrez T 642 Mussmann M
Gutierrez T 642 Wasmund K
Gutierrez T 642 Herbold CW
Gutierrez T 642 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Yang T 783 Horn M
Yang T 783 Daims H
Yang T 783 Berry D
Yang T 783 Loy A
Yang T 783 Wagner M
Yang T 783 Eichorst SA
Yang T 783 Mussmann M
Yang T 783 Wasmund K
Yang T 783 Herbold CW
Yang T 783 Sedlacek CJ
Mishamandani S 644 Horn M
Mishamandani S 644 Daims H
Mishamandani S 644 Berry D
Mishamandani S 644 Loy A
Mishamandani S 644 Wagner M
Mishamandani S 644 Eichorst SA
Mishamandani S 644 Mussmann M
Mishamandani S 644 Wasmund K
Mishamandani S 644 Herbold CW
Mishamandani S 644 Sedlacek CJ
McKay L 784 Horn M
McKay L 784 Daims H
McKay L 784 Berry D
McKay L 784 Loy A
McKay L 784 Wagner M
McKay L 784 Eichorst SA
McKay L 784 Mussmann M
McKay L 784 Wasmund K
McKay L 784 Herbold CW
McKay L 784 Sedlacek CJ
Teske A 785 Horn M
Teske A 785 Daims H
Teske A 785 Berry D
Teske A 785 Loy A
Teske A 785 Wagner M
Teske A 785 Eichorst SA
Teske A 785 Mussmann M
Teske A 785 Wasmund K
Teske A 785 Herbold CW
Teske A 785 Sedlacek CJ
Aitken MD 647 Horn M
Aitken MD 647 Daims H
Aitken MD 647 Berry D
Aitken MD 647 Loy A
Aitken MD 647 Wagner M
Aitken MD 647 Eichorst SA
Aitken MD 647 Mussmann M
Aitken MD 647 Wasmund K
Aitken MD 647 Herbold CW
Aitken MD 647 Sedlacek CJ
Holder D 776 Horn M
Holder D 776 Daims H
Holder D 776 Berry D
Holder D 776 Loy A
Holder D 776 Wagner M
Holder D 776 Eichorst SA
Holder D 776 Mussmann M
Holder D 776 Wasmund K
Holder D 776 Herbold CW
Holder D 776 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Dai D 777 Horn M
Dai D 777 Daims H
Dai D 777 Berry D
Dai D 777 Loy A
Dai D 777 Wagner M
Dai D 777 Eichorst SA
Dai D 777 Mussmann M
Dai D 777 Wasmund K
Dai D 777 Herbold CW
Dai D 777 Sedlacek CJ
Raskin L 683 Horn M
Raskin L 683 Daims H
Raskin L 683 Berry D
Raskin L 683 Loy A
Raskin L 683 Wagner M
Raskin L 683 Eichorst SA
Raskin L 683 Mussmann M
Raskin L 683 Wasmund K
Raskin L 683 Herbold CW
Raskin L 683 Sedlacek CJ
Xi C 778 Horn M
Xi C 778 Daims H
Xi C 778 Berry D
Xi C 778 Loy A
Xi C 778 Wagner M
Xi C 778 Eichorst SA
Xi C 778 Mussmann M
Xi C 778 Wasmund K
Xi C 778 Herbold CW
Xi C 778 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Schwab C 513 Horn M
Schwab C 513 Daims H
Schwab C 513 Berry D
Schwab C 513 Loy A
Schwab C 513 Wagner M
Schwab C 513 Eichorst SA
Schwab C 513 Mussmann M
Schwab C 513 Wasmund K
Schwab C 513 Herbold CW
Schwab C 513 Sedlacek CJ
Milinovich G 1037 Horn M
Milinovich G 1037 Daims H
Milinovich G 1037 Berry D
Milinovich G 1037 Loy A
Milinovich G 1037 Wagner M
Milinovich G 1037 Eichorst SA
Milinovich G 1037 Mussmann M
Milinovich G 1037 Wasmund K
Milinovich G 1037 Herbold CW
Milinovich G 1037 Sedlacek CJ
Reichert J 802 Horn M
Reichert J 802 Daims H
Reichert J 802 Berry D
Reichert J 802 Loy A
Reichert J 802 Wagner M
Reichert J 802 Eichorst SA
Reichert J 802 Mussmann M
Reichert J 802 Wasmund K
Reichert J 802 Herbold CW
Reichert J 802 Sedlacek CJ
Ben Mahfoudh K 1038 Horn M
Ben Mahfoudh K 1038 Daims H
Ben Mahfoudh K 1038 Berry D
Ben Mahfoudh K 1038 Loy A
Ben Mahfoudh K 1038 Wagner M
Ben Mahfoudh K 1038 Eichorst SA
Ben Mahfoudh K 1038 Mussmann M
Ben Mahfoudh K 1038 Wasmund K
Ben Mahfoudh K 1038 Herbold CW
Ben Mahfoudh K 1038 Sedlacek CJ
Decker T 287 Horn M
Decker T 287 Daims H
Decker T 287 Berry D
Decker T 287 Loy A
Decker T 287 Wagner M
Decker T 287 Eichorst SA
Decker T 287 Mussmann M
Decker T 287 Wasmund K
Decker T 287 Herbold CW
Decker T 287 Sedlacek CJ
Engel M 1039 Horn M
Engel M 1039 Daims H
Engel M 1039 Berry D
Engel M 1039 Loy A
Engel M 1039 Wagner M
Engel M 1039 Eichorst SA
Engel M 1039 Mussmann M
Engel M 1039 Wasmund K
Engel M 1039 Herbold CW
Engel M 1039 Sedlacek CJ
Hai B 1040 Horn M
Hai B 1040 Daims H
Hai B 1040 Berry D
Hai B 1040 Loy A
Hai B 1040 Wagner M
Hai B 1040 Eichorst SA
Hai B 1040 Mussmann M
Hai B 1040 Wasmund K
Hai B 1040 Herbold CW
Hai B 1040 Sedlacek CJ
Hainzl E 510 Horn M
Hainzl E 510 Daims H
Hainzl E 510 Berry D
Hainzl E 510 Loy A
Hainzl E 510 Wagner M
Hainzl E 510 Eichorst SA
Hainzl E 510 Mussmann M
Hainzl E 510 Wasmund K
Hainzl E 510 Herbold CW
Hainzl E 510 Sedlacek CJ
Heider S 512 Horn M
Heider S 512 Daims H
Heider S 512 Berry D
Heider S 512 Loy A
Heider S 512 Wagner M
Heider S 512 Eichorst SA
Heider S 512 Mussmann M
Heider S 512 Wasmund K
Heider S 512 Herbold CW
Heider S 512 Sedlacek CJ
Kenner L 518 Horn M
Kenner L 518 Daims H
Kenner L 518 Berry D
Kenner L 518 Loy A
Kenner L 518 Wagner M
Kenner L 518 Eichorst SA
Kenner L 518 Mussmann M
Kenner L 518 Wasmund K
Kenner L 518 Herbold CW
Kenner L 518 Sedlacek CJ
Müller M 516 Horn M
Müller M 516 Daims H
Müller M 516 Berry D
Müller M 516 Loy A
Müller M 516 Wagner M
Müller M 516 Eichorst SA
Müller M 516 Mussmann M
Müller M 516 Wasmund K
Müller M 516 Herbold CW
Müller M 516 Sedlacek CJ
Rauch I 286 Horn M
Rauch I 286 Daims H
Rauch I 286 Berry D
Rauch I 286 Loy A
Rauch I 286 Wagner M
Rauch I 286 Eichorst SA
Rauch I 286 Mussmann M
Rauch I 286 Wasmund K
Rauch I 286 Herbold CW
Rauch I 286 Sedlacek CJ
Strobl B 517 Horn M
Strobl B 517 Daims H
Strobl B 517 Berry D
Strobl B 517 Loy A
Strobl B 517 Wagner M
Strobl B 517 Eichorst SA
Strobl B 517 Mussmann M
Strobl B 517 Wasmund K
Strobl B 517 Herbold CW
Strobl B 517 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Schleper C 405 Horn M
Schleper C 405 Daims H
Schleper C 405 Berry D
Schleper C 405 Loy A
Schleper C 405 Wagner M
Schleper C 405 Eichorst SA
Schleper C 405 Mussmann M
Schleper C 405 Wasmund K
Schleper C 405 Herbold CW
Schleper C 405 Sedlacek CJ
Urich T 515 Horn M
Urich T 515 Daims H
Urich T 515 Berry D
Urich T 515 Loy A
Urich T 515 Wagner M
Urich T 515 Eichorst SA
Urich T 515 Mussmann M
Urich T 515 Wasmund K
Urich T 515 Herbold CW
Urich T 515 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Ben Mahfoudh K 1038 Horn M
Ben Mahfoudh K 1038 Daims H
Ben Mahfoudh K 1038 Berry D
Ben Mahfoudh K 1038 Loy A
Ben Mahfoudh K 1038 Wagner M
Ben Mahfoudh K 1038 Eichorst SA
Ben Mahfoudh K 1038 Mussmann M
Ben Mahfoudh K 1038 Wasmund K
Ben Mahfoudh K 1038 Herbold CW
Ben Mahfoudh K 1038 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Steger D 852 Horn M
Steger D 852 Daims H
Steger D 852 Berry D
Steger D 852 Loy A
Steger D 852 Wagner M
Steger D 852 Eichorst SA
Steger D 852 Mussmann M
Steger D 852 Wasmund K
Steger D 852 Herbold CW
Steger D 852 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Haider S 958 Horn M
Haider S 958 Daims H
Haider S 958 Berry D
Haider S 958 Loy A
Haider S 958 Wagner M
Haider S 958 Eichorst SA
Haider S 958 Mussmann M
Haider S 958 Wasmund K
Haider S 958 Herbold CW
Haider S 958 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Wagner M 273 Horn M
Wagner M 273 Daims H
Wagner M 273 Berry D
Wagner M 273 Loy A
Wagner M 273 Wagner M
Wagner M 273 Eichorst SA
Wagner M 273 Mussmann M
Wagner M 273 Wasmund K
Wagner M 273 Herbold CW
Wagner M 273 Sedlacek CJ
Stocker R 1129 Horn M
Stocker R 1129 Daims H
Stocker R 1129 Berry D
Stocker R 1129 Loy A
Stocker R 1129 Wagner M
Stocker R 1129 Eichorst SA
Stocker R 1129 Mussmann M
Stocker R 1129 Wasmund K
Stocker R 1129 Herbold CW
Stocker R 1129 Sedlacek CJ
Loy A 306 Horn M
Loy A 306 Daims H
Loy A 306 Berry D
Loy A 306 Loy A
Loy A 306 Wagner M
Loy A 306 Eichorst SA
Loy A 306 Mussmann M
Loy A 306 Wasmund K
Loy A 306 Herbold CW
Loy A 306 Sedlacek CJ
Berry D 275 Horn M
Berry D 275 Daims H
Berry D 275 Berry D
Berry D 275 Loy A
Berry D 275 Wagner M
Berry D 275 Eichorst SA
Berry D 275 Mussmann M
Berry D 275 Wasmund K
Berry D 275 Herbold CW
Berry D 275 Sedlacek CJ
Horn M 264 Horn M
Horn M 264 Daims H
Horn M 264 Berry D
Horn M 264 Loy A
Horn M 264 Wagner M
Horn M 264 Eichorst SA
Horn M 264 Mussmann M
Horn M 264 Wasmund K
Horn M 264 Herbold CW
Horn M 264 Sedlacek CJ
Xi C 778 Horn M
Xi C 778 Daims H
Xi C 778 Berry D
Xi C 778 Loy A
Xi C 778 Wagner M
Xi C 778 Eichorst SA
Xi C 778 Mussmann M
Xi C 778 Wasmund K
Xi C 778 Herbold CW
Xi C 778 Sedlacek CJ
Raskin L 683 Horn M
Raskin L 683 Daims H
Raskin L 683 Berry D
Raskin L 683 Loy A
Raskin L 683 Wagner M
Raskin L 683 Eichorst SA
Raskin L 683 Mussmann M
Raskin L 683 Wasmund K
Raskin L 683 Herbold CW
Raskin L 683 Sedlacek CJ
Publications | Microbial Ecology, University of Vienna

Publications

Publications in peer reviewed journals

45 Publications found
  • Fluorinated Gold Nanoparticles for Nanostructure Imaging Mass Spectrometry.

    Palermo A, Forsberg EM, Warth B, Aisporna AE, Billings E, Kuang E, Benton HP, Berry D, Siuzdak G
    2018 - ACS Nano, in press

    Abstract: 

    Nanostructure imaging mass spectrometry (NIMS) with fluorinated gold nanoparticles (f-AuNPs) is a nanoparticle assisted laser desorption/ionization approach that requires low laser energy and has demonstrated high sensitivity. Here we describe NIMS with f-AuNPs for the comprehensive analysis of metabolites in biological tissues. F-AuNPs assist in desorption/ionization by laser-induced release of the fluorocarbon chains with minimal background noise. Since the energy barrier required to release the fluorocarbons from the AuNPs is minimal, the energy of the laser is maintained in the low μJ/pulse range, thus limiting metabolite in-source fragmentation. Electron microscopy analysis of tissue samples after f-AuNP NIMS shows a distinct "raising" of the surface as compared to matrix assisted laser desorption ionization ablation, indicative of a gentle desorption mechanism aiding in the generation of intact molecular ions. Moreover, the use of perfluorohexane to distribute the f-AuNPs on the tissue creates a hydrophobic environment minimizing metabolite solubilization and spatial dislocation. The transfer of the energy from the incident laser to the analytes through the release of the fluorocarbon chains similarly enhances the desorption/ionization of metabolites of different chemical nature, resulting in heterogeneous metabolome coverage. We performed the approach in a comparative study of the colon of mice exposed to three different diets. F-AuNP NIMS allows the direct detection of carbohydrates, lipids, bile acids, sulfur metabolites, amino acids, nucleotide precursors as well as other small molecules of varied biological origins. Ultimately, the diversified molecular coverage obtained provides a broad picture of a tissue's metabolic organization.

  • Long-distance electron transport in individual, living cable bacteria.

    Bjerg JT, Boschker HTS, Larsen S, Berry D, Schmid M, Millo D, Tataru P, Meysman FJR, Wagner M, Nielsen LP, Schramm A
    2018 - Proc. Natl. Acad. Sci. U.S.A., 22: 5786-5791

    Abstract: 

    Electron transport within living cells is essential for energy conservation in all respiring and photosynthetic organisms. While a few bacteria transport electrons over micrometer distances to their surroundings, filaments of cable bacteria are hypothesized to conduct electric currents over centimeter distances. We used resonance Raman microscopy to analyze cytochrome redox states in living cable bacteria. Cable-bacteria filaments were placed in microscope chambers with sulfide as electron source and oxygen as electron sink at opposite ends. Along individual filaments a gradient in cytochrome redox potential was detected, which immediately broke down upon removal of oxygen or laser cutting of the filaments. Without access to oxygen, a rapid shift toward more reduced cytochromes was observed, as electrons were no longer drained from the filament but accumulated in the cellular cytochromes. These results provide direct evidence for long-distance electron transport in living multicellular bacteria.

  • Vitamin and Amino Acid Auxotrophy in Anaerobic Consortia Operating under Methanogenic Conditions.

    Hubalek V, Buck M, Tan B, Foght J, Wendeberg A, Berry D, Bertilsson S, Eiler A
    2017 - mSystems, 5: e00038-17

    Abstract: 

    Syntrophy among Archaea and Bacteria facilitates the anaerobic degradation of organic compounds to CH4 and CO2. Particularly during aliphatic and aromatic hydrocarbon mineralization, as in the case of crude oil reservoirs and petroleum-contaminated sediments, metabolic interactions between obligate mutualistic microbial partners are of central importance. Using micromanipulation combined with shotgun metagenomic approaches, we describe the genomes of complex consortia within short-chain alkane-degrading cultures operating under methanogenic conditions. Metabolic reconstruction revealed that only a small fraction of genes in the metagenome-assembled genomes encode the capacity for fermentation of alkanes facilitated by energy conservation linked to H2 metabolism. Instead, the presence of inferred lifestyles based on scavenging anabolic products and intermediate fermentation products derived from detrital biomass was a common feature. Additionally, inferred auxotrophy for vitamins and amino acids suggests that the hydrocarbon-degrading microbial assemblages are structured and maintained by multiple interactions beyond the canonical H2-producing and syntrophic alkane degrader-methanogen partnership. Compared to previous work, our report points to a higher order of complexity in microbial consortia engaged in anaerobic hydrocarbon transformation. IMPORTANCE Microbial interactions between Archaea and Bacteria mediate many important chemical transformations in the biosphere from degrading abundant polymers to synthesis of toxic compounds. Two of the most pressing issues in microbial interactions are how consortia are established and how we can modulate these microbial communities to express desirable functions. Here, we propose that public goods (i.e., metabolites of high energy demand in biosynthesis) facilitate energy conservation for life under energy-limited conditions and determine the assembly and function of the consortia. Our report suggests that an understanding of public good dynamics could result in new ways to improve microbial pollutant degradation in anaerobic systems.

  • Bottled aqua incognita: Microbiota assembly and dissolved organic matter diversity in natural mineral waters

    Lesaulnier CC, Herbold CW, Pelikan C, Gérard C, Le Coz X, Gagnot S, Berry D, Niggemann J, Dittmar T, Singer GA, Loy A
    2017 - Microbiome, 5: 126

    Abstract: 

    Background: Non-carbonated natural mineral waters contain microorganisms that regularly grow after bottling despite low concentrations of dissolved organic matter (DOM). Yet, the compositions of bottled water microbiota and organic substrates that fuel microbial activity, and how both change after bottling, are still largely unknown.

    Results: We performed a multifaceted analysis of microbiota and DOM diversity in twelve natural mineral waters from six European countries. 16S rRNA gene-based analyses showed that less than ten species-level operational taxonomic units (OTUs) dominated the bacterial communities in the water phase and associated with the bottle wall after a short phase of post-bottling growth. Members of the betaproteobacterial genera Curvibacter, Aquabacterium, and Polaromonas (Comamonadaceae) grew in most waters and represent ubiquitous, mesophilic, heterotrophic aerobes in bottled waters. Ultrahigh-resolution mass spectrometry of DOM in bottled waters and their corresponding source waters identified thousands of molecular formulae characteristic of mostly refractory, soil-derived DOM.

    Conclusions. The bottle environment, including source water physicochemistry, selected for growth of a similar low-diversity microbiota across various bottled waters. Relative abundance changes of hundreds of multi-carbon molecules were related to growth of less than ten abundant OTUs. We thus speculate that individual bacteria cope with oligotrophic conditions by simultaneously consuming diverse DOM molecules.

  • Allspice and Clove As Source of Triterpene Acids Activating the G Protein-Coupled Bile Acid Receptor TGR5.

    Ladurner A, Zehl M, Grienke U, Hofstadler C, Faur N, Pereira FC, Berry D, Dirsch VM, Rollinger JM
    2017 - Front Pharmacol, 8: 468

    Abstract: 

    Worldwide, metabolic diseases such as obesity and type 2 diabetes have reached epidemic proportions. A major regulator of metabolic processes that gained interest in recent years is the bile acid receptor TGR5 (Takeda G protein-coupled receptor 5). This G protein-coupled membrane receptor can be found predominantly in the intestine, where it is mainly responsible for the secretion of the incretins glucagon-like peptide 1 (GLP-1) and peptide YY (PYY). The aim of this study was (i) to identify plant extracts with TGR5-activating potential, (ii) to narrow down their activity to the responsible constituents, and (iii) to assess whether the intestinal microbiota produces transformed metabolites with a different activity profile. Chenodeoxycholic acid (CDCA) served as positive control for both, the applied cell-based luciferase reporter gene assay for TGR5 activity and the biotransformation assay using mouse fecal slurry. The suitability of the workflow was demonstrated by the biotransformation of CDCA to lithocholic acid resulting in a distinct increase in TGR5 activity. Based on a traditional Tibetan formula, 19 plant extracts were selected and investigated for TGR5 activation. Extracts from the commonly used spices Syzygium aromaticum (SaroE, clove), Pimenta dioica (PdioE, allspice), and Kaempferia galanga (KgalE, aromatic ginger) significantly increased TGR5 activity. After biotransformation, only KgalE showed significant differences in its metabolite profile, which, however, did not alter its TGR5 activity compared to non-transformed KgalE. UHPLC-HRMS (high-resolution mass spectrometry) analysis revealed triterpene acids (TTAs) as the main constituents of the extracts SaroE and PdioE. Identification and quantification of TTAs in these two extracts as well as comparison of their TGR5 activity with reconstituted TTA mixtures allowed the attribution of the TGR5 activity to TTAs. EC50s were determined for the main TTAs, i.e., oleanolic acid (2.2 ± 1.6 μM), ursolic acid (1.1 ± 0.2 μM), as well as for the hitherto unknown TGR5 activators corosolic acid (0.5 ± 1.0 μM) and maslinic acid (3.7 ± 0.7 μM). In conclusion, extracts of clove, allspice, and aromatic ginger activate TGR5, which might play a pivotal role in their therapeutic use for the treatment of metabolic diseases. Moreover, the TGR5 activation of SaroE and PdioE could be pinpointed solely to TTAs.

  • Evaluating the Detection of Hydrocarbon-Degrading Bacteria in 16S rRNA Gene Sequencing Surveys.

    Berry D, Gutierrez T
    2017 - Front Microbiol, 8: 2460

    Abstract: 

    Hydrocarbonoclastic bacteria (HCB) play a key role in the biodegradation of oil hydrocarbons in marine and other environments. A small number of taxa have been identified as obligate HCB, notably the Gammaproteobacterial genera Alcanivorax, Cycloclasticus, Marinobacter, Neptumonas, Oleiphilus, Oleispira, and Thalassolituus, as well as the Alphaproteobacterial genus Thalassospira. Detection of HCB in amplicon-based sequencing surveys relies on high coverage by PCR primers and accurate taxonomic classification. In this study, we performed a phylogenetic analysis to identify 16S rRNA gene sequence regions that represent the breadth of sequence diversity within these taxa. Using validated sequences, we evaluated 449 universal 16S rRNA gene-targeted bacterial PCR primer pairs for their coverage of these taxa. The results of this analysis provide a practical framework for selection of suitable primer sets for optimal detection of HCB in sequencing surveys.

  • Vibrational Spectroscopy for Imaging Single Microbial Cells in Complex Biological Samples.

    Harrison JP, Berry D
    2017 - Front Microbiol, 8: 675

    Abstract: 

    Vibrational spectroscopy is increasingly used for the rapid and non-destructive imaging of environmental and medical samples. Both Raman and Fourier-transform infrared (FT-IR) imaging have been applied to obtain detailed information on the chemical composition of biological materials, ranging from single microbial cells to tissues. Due to its compatibility with methods such as stable isotope labeling for the monitoring of cellular activities, vibrational spectroscopy also holds considerable power as a tool in microbial ecology. Chemical imaging of undisturbed biological systems (such as live cells in their native habitats) presents unique challenges due to the physical and chemical complexity of the samples, potential for spectral interference, and frequent need for real-time measurements. This Mini Review provides a critical synthesis of recent applications of Raman and FT-IR spectroscopy for characterizing complex biological samples, with a focus on developments in single-cell imaging. We also discuss how new spectroscopic methods could be used to overcome current limitations of single-cell analyses. Given the inherent complementarity of Raman and FT-IR spectroscopic methods, we discuss how combining these approaches could enable us to obtain new insights into biological activities either in situ or under conditions that simulate selected properties of the natural environment.

  • HuR small-molecule inhibitor elicits differential effects in adenomatosis polyposis and colorectal carcinogenesis

    Lang M, Berry D, Passecker K, Mesteri I, Bhuju S, Ebner F, Sedlyarov V, Evstatiev R, Dammann K, Loy A, Kuzyk O, Kovarik P, Khare V, Beibel M, Roma G, Meisner-Kober N, Gasche C
    2017 - Cancer Res., 77: 2424-2438

    Abstract: 

    HuR is an RNA-binding protein implicated in immune homeostasis and various cancers, including colorectal cancer. HuR binding to AU-rich elements within the 3' untranslated region of mRNAs encoding oncogenes, growth factors, and various cytokines leads message stability and translation. In this study, we evaluated HuR as a small-molecule target for preventing colorectal cancer in high-risk groups such as those with familial adenomatosis polyposis (FAP) or inflammatory bowel disease (IBD). In human specimens, levels of cytoplasmic HuR were increased in colonic epithelial cells from patients with IBD, IBD-cancer, FAP-adenoma, and colorectal cancer, but not in patients with IBD-dysplasia. Intraperitoneal injection of the HuR small-molecule inhibitor MS-444 in AOM/DSS mice, a model of IBD and inflammatory colon cancer, augmented DSS-induced weight loss and increased tumor multiplicity, size, and invasiveness. MS-444 treatment also abrogated tumor cell apoptosis and depleted tumor-associated eosinophils, accompanied by a decrease in IL18 and eotaxin-1. In contrast, HuR inhibition in APCMin mice, a model of FAP and colon cancer, diminished the number of small intestinal tumors generated. In this setting, fecal microbiota, evaluated by 16S rRNA gene amplicon sequencing, shifted to a state of reduced bacterial diversity, with an increased representation of Prevotella, Akkermansia, and Lachnospiraceae Taken together, our results indicate that HuR activation is an early event in FAP-adenoma but is not present in IBD-dysplasia. Furthermore, our results offer a preclinical proof of concept for HuR inhibition as an effective means of FAP chemoprevention, with caution advised in the setting of IBD.

  • Members of the Oral Microbiota Are Associated with IL-8 Release by Gingival Epithelial Cells in Healthy Individuals.

    Schueller K, Riva A, Pfeiffer S, Berry D, Somoza V
    2017 - Front Microbiol, 8: 416

    Abstract: 

    The triggers for the onset of oral diseases are still poorly understood. The aim of this study was to characterize the oral bacterial community in healthy humans and its association with nutrition, oral hygiene habits, and the release of the inflammatory marker IL-8 from gingival epithelial cells (GECs) with and without stimulation by bacterial endotoxins to identify possible indicator operational taxonomic units (OTUs) associated with inflammatory marker status. GECs from 21 healthy participants (13 females, 8 males) were incubated with or without addition of bacterial lipopolysaccharides (LPSs), and the oral microbiota was profiled using 16S rRNA gene-targeted sequencing. The basal IL-8 release after 6 h was between 9.9 and 98.2 pg/ml, and bacterial communities were characteristic for healthy oral microbiota. The composition of the oral microbiota was associated with basal IL-8 levels, the intake of meat, tea, white wine, sweets and the use of chewing gum, as well as flossing habits, allergies, gender and body mass index. Additionally, eight OTUs were associated with high basal levels of IL-8 and GEC response to LPS, with high basal levels of IL-8, and 1 with low basal levels of IL8. The identification of indicator bacteria in healthy subjects with high levels of IL-8 release is of importance as they may be promising early warning indicators for the possible onset of oral diseases.

  • Lifestyle and horizontal gene transfer-mediated evolution of Mucispirillum schaedleri, a core member of the murine gut microbiota

    Loy A, Pfann C, Steinberger M, Hanson B, Herp S, Brugiroux S, Gomes Neto JC, Boekschoten MV, Schwab C, Urich T, Ramer-Tait AE, Rattei T, Stecher B, Berry D
    2017 - mSystems, 2: e00171-16

    Abstract: 

    Mucispirillum schaedleri is an abundant inhabitant of the intestinal mucus layer of rodents and other animals and has been suggested to be a pathobiont, a commensal that plays a role in disease. In order to gain insights into its lifestyle, we analyzed the genome and transcriptome of M. schaedleri ASF 457 and performed physiological experiments to test traits predicted by its genome. Although described as a mucus inhabitant, M. schaedleri has limited capacity for degrading host-derived mucosal glycans and other complex polysaccharides. Additionally, M. schaedleri reduces nitrate and expresses systems for scavenging oxygen and reactive oxygen species in vivo, which may account for its localization close to the mucosal tissue and expansion during inflammation. Also of note, M. schaedleri harbors a type VI secretion system and putative effector proteins and can modify gene expression in mucosal tissue, suggesting intimate interactions with its host and a possible role in inflammation. The M. schaedleri genome has been shaped by extensive horizontal gene transfer, primarily from intestinal Epsilon- and Deltaproteobacteria, indicating that horizontal gene transfer has played a key role in defining its niche in the gut ecosystem.

  • Microbial nutrient niches in the gut.

    Pereira FC, Berry D
    2017 - Environ. Microbiol., 4: 1366-1378

    Abstract: 

    The composition and function of the mammalian gut microbiota has been the subject of much research in recent years, but the principles underlying the assembly and structure of this complex community remain incompletely understood. Processes that shape the gut microbiota are thought to be mostly niche-driven, with environmental factors such as the composition of available nutrients largely determining whether or not an organism can establish. The concept that the nutrient landscape dictates which organisms can successfully colonize and persist in the gut was first proposed in Rolf Freter's nutrient niche theory. In a situation where nutrients are perfectly mixed and there is balanced microbial growth, Freter postulated that an organism can only survive if it is able to utilize one or a few limiting nutrients more efficiently than its competitors. Recent experimental work indicates, however, that nutrients in the gut vary in space and time. We propose that in such a scenario, Freter's nutrient niche theory must be expanded to account for the co-existence of microorganisms utilizing the same nutrients but in distinct sites or at different times, and that metabolic flexibility and mixed-substrate utilization are common strategies for survival in the face of ever-present nutrient fluctuations.

  • A 12-week intervention with nonivamide, a TRPV1 agonist, prevents a dietary-induced body fat gain and increases peripheral serotonin in moderately overweight subjects.

    Hochkogler CM, Lieder B, Rust P, Berry D, Meier SM, Pignitter M, Riva A, Leitinger A, Bruk A, Wagner S, Hans J, Widder S, Ley JP, Krammer GE, Somoza V
    2017 - Mol Nutr Food Res, 5: 1600731

    Abstract: 

    A bolus administration of 0.15 mg nonivamide has previously been demonstrated to reduce energy intake in moderately overweight men. This 12-week intervention investigated whether a daily consumption of nonivamide in a protein-based product formulation promotes a reduction in body weight in healthy overweight subjects and affects outcome measures associated with mechanisms regulating food intake, e.g. plasma concentrations of (an)orexigenic hormones, energy substrates as well as changes in fecal microbiota.
    Nineteen overweight subjects were randomly assigned to either a control (C) or a nonivamide (NV) group. Changes in the body composition and plasma concentrations of satiating hormones were determined at fasting and 15, 30, 60, 90, and 120 min after a glucose load. Participants were instructed to consume 0.15 mg nonivamide per day in 450 mL of a milk shake additionally to their habitual diet. After treatment, a group difference in body fat mass change (-0.61 ± 0.36% in NV and +1.36 ± 0.38% in C) and an increase in postprandial plasma serotonin were demonstrated. Plasma metabolome and fecal microbiome read outs were not affected.
    A daily intake of 0.15 mg nonivamide helps to support to maintain a healthy body composition.

  • Pediatric obesity is associated with an altered gut microbiota and discordant shifts in Firmicutes populations

    Riva A, Borgo F, Lassandro C, Verduci E, Morace G, Borghi E, Berry D
    2017 - Environ. Microbiol., 1: 95-105

    Abstract: 

    An altered gut microbiota has been linked to obesity in adulthood, although little is known about childhood obesity. The aim of this study was to characterize the composition of the gut microbiota in obese (n = 42) and normal-weight (n = 36) children aged 6 to 16. Using 16S rRNA gene-targeted sequencing, we evaluated taxa with differential abundance according to age- and sex-normalized body mass index (BMI z-score). Obesity was associated with an altered gut microbiota characterized by elevated levels of Firmicutes and depleted levels of Bacteroidetes. Correlation network analysis revealed that the gut microbiota of obese children also had increased correlation density and clustering of operational taxonomic units (OTUs). Members of the Bacteroidetes were generally better predictors of BMI z-score and obesity than Firmicutes, which was likely due to discordant responses of Firmicutes OTUs. In accordance with these observations, the main metabolites produced by gut bacteria, short chain fatty acids (SCFAs), were higher in obese children, suggesting elevated substrate utilisation. Multiple taxa were correlated with SCFA levels, reinforcing the tight link between the microbiota, SCFAs and obesity. Our results suggest that gut microbiota dysbiosis and elevated fermentation activity may be involved in the etiology of childhood obesity.

  • Genome-guided design of a novel defined mouse microbiota that confers colonization resistance against Salmonella enterica serovar Typhimurium

    Brugiroux S, Beutler M, Pfann C, Garzetti D, Ruscheweyh H-J, Ring D, Diehl M, Herp S, Lötscher Y, Hussain S, Bunk B, Pukall R, Huson DH, Münch PC, McHardy AC, McCoy KD, Macpherson AJ, Loy A, Clavel T, Berry D, Stecher B
    2016 - Nature Microbiol, 2: 16215

    Abstract: 

    Protection against enteric infections, also termed colonization resistance, results from mutualistic interactions of the host and its indigenous microbes. The gut microbiota of humans and mice is highly diverse and it is therefore challenging to assign specific properties to its individual members. Here, we have used a collection of murine bacterial strains and a modular design approach to create a minimal bacterial community that, once established in germ-free mice, provided colonization resistance against the human enteric pathogen Salmonella enterica serovar Typhimurium (S. Tm). Initially, a community of 12 strains, termed Oligo-Mouse Microbiota (Oligo-MM12), representing members of the major bacterial phyla in the murine gut, was selected. This community was stable over consecutive mouse generations and provided colonization resistance against S. Tm infection, albeit not to the degree of a conventional complex microbiota. Comparative (meta)genome analyses identified functions represented in a conventional microbiome but absent from the Oligo-MM12. By genome-informed design, we created an improved version of the Oligo-MM community harbouring three facultative anaerobic bacteria from the Mouse Intestinal Bacterial Collection (miBC) that provided conventional-like colonization resistance. In conclusion, we have established a highly versatile experimental system that showed efficacy in an enteric infection model. Thus, in combination with exhaustive bacterial strain collections and systems-based approaches, genomeguided design can be used to generate insights into microbe–microbe and microbe–host interactions for the investigation of ecological and disease-relevant mechanisms in the intestine.

  • Bacterial nutrient foraging in a mouse model of enteral nutrient deprivation: Insight into the gut origin of sepsis

    Ralls MW, Demehri FR, Feng Y, Raskind S, Ruan C, Schintlmeister A, Loy A, Hanson B, Berry D, Burant CF, Teitelbaum DH
    2016 - Am J Physiol Gastrointest Liver Physiol, 311: G734-G743

    Abstract: 

    Total parenteral nutrition (TPN) leads to a shift in small intestinal microbiota with a characteristic dominance of Proteobacteria. This study examined how metabolomic changes within the small bowel support an altered microbial community in enterally deprived mice.
    C57BL/6 mice were given TPN or enteral chow. Metabolomic analysis of jejunal contents was performed by liquid chromatography/mass spectrometry (LC/MS). In some experiments, leucine in TPN was partly substituted with (13)C-leucine. Additionally, jejunal contents from TPN dependent and enterally fed mice were gavaged into germ-free mice to reveal if the TPN phenotype was transferrable.
    Small bowel contents of TPN mice maintained an amino acid composition similar to that of the TPN solution. Mass spectrometry analysis of small bowel contents of TPN dependent mice showed increased concentration of (13)C compared to fed mice receiving saline enriched with (13)C-leucine. (13)C-leucine added to the serosal side of Ussing chambers showed rapid permeation across TPN-dependent jejunum, suggesting increased transmucosal passage. Single-cell analysis by fluorescence in situ hybridization (FISH) - NanoSIMS demonstrated uptake of (13)C-leucine by TPN-associated bacteria, with preferential uptake by Enterobacteriaceae. Gavage of small bowel effluent from TPN mice into germ-free, fed mice resulted in a trend toward the pro-inflammatory TPN-phenotype with loss of epithelial barrier function.
    TPN-dependence leads to increased permeation of TPN-derived nutrients into the small intestinal lumen, where they are predominately utilized by Enterobacteriaceae. The altered metabolomic composition of the intestinal lumen during TPN promotes dysbiosis.

  • Behavior of platinum(iv) complexes in models of tumor hypoxia: cytotoxicity, compound distribution and accumulation

    Schreiber-Brynzak E, Pichler V, Heffeter P, Hanson B, Theiner S, Lichtscheidl-Schultz I, Kornauth C, Bamonti L, Dhery V, Groza D, Berry D, Berger W, Galanski M, Jakupec MA, Keppler BK
    2016 - Metallomics, 4: 422-33

    Abstract: 

    Hypoxia in solid tumors remains a challenge for conventional cancer therapeutics. As a source for resistance, metastasis development and drug bioprocessing, it influences treatment results and disease outcome. Bioreductive platinum(iv) prodrugs might be advantageous over conventional metal-based therapeutics, as biotransformation in a reductive milieu, such as under hypoxia, is required for drug activation. This study deals with a two-step screening of experimental platinum(iv) prodrugs with different rates of reduction and lipophilicity with the aim of identifying the most appropriate compounds for further investigations. In the first step, the cytotoxicity of all compounds was compared in hypoxic multicellular spheroids and monolayer culture using a set of cancer cell lines with different sensitivities to platinum(ii) compounds. Secondly, two selected compounds were tested in hypoxic xenografts in SCID mouse models in comparison to satraplatin, and, additionally, (LA)-ICP-MS-based accumulation and distribution studies were performed for these compounds in hypoxic spheroids and xenografts. Our findings suggest that, while cellular uptake and cytotoxicity strongly correlate with lipophilicity, cytotoxicity under hypoxia compared to non-hypoxic conditions and antitumor activity of platinum(iv) prodrugs are dependent on their rate of reduction.

  • Activity and community structures of sulfate-reducing microorganisms in polar, temperate and tropical marine sediments

    Robador A, Müller AL, Sawicka JE, Berry D, Hubert CRJ, Loy A, Jørgensen BB, Brüchert V
    2016 - ISME J, 10: 796–809

    Abstract: 

    Temperature has a fundamental impact on the metabolic rates of microorganisms and strongly influences microbial ecology and biogeochemical cycling in the environment. In this study, we examined the catabolic temperature response of natural communities of sulfate-reducing microorganisms (SRM) in polar, temperate, and tropical marine sediments. In short-term sediment incubation experiments with 35S-sulfate, we demonstrated how the cardinal temperatures for sulfate reduction correlate with mean annual sediment temperatures, indicating specific thermal adaptations of the dominant SRM in each of the investigated ecosystems. The community structure of putative SRM in the sediments, as revealed by pyrosequencing of bacterial 16S rRNA gene amplicons and phylogenetic assignment to known SRM taxa, consistently correlated with in situ temperatures, but not with sediment organic carbon concentrations or C:N ratios of organic matter. Additionally, several species-level SRM phylotypes of the class Deltaproteobacteria tended to co-occur at sites with similar mean annual temperatures, regardless of geographic distance. The observed temperature adaptations of SRM imply that environmental temperature is a major controlling variable for physiological selection and ecological and evolutionary differentiation of microbial communities.

  • Response of the bacterial community associated with a cosmopolitan marine diatom to crude oil shows a preference for the biodegradation of aromatic hydrocarbons.

    Mishamandani S, Gutierrez T, Berry D, Aitken MD
    2016 - Environ. Microbiol., 6: 1817-33

    Abstract: 

    Emerging evidence shows that hydrocarbonoclastic bacteria (HCB) may be commonly found associated with phytoplankton in the ocean, but the ecology of these bacteria and how they respond to crude oil remains poorly understood. Here, we used a natural diatom-bacterial assemblage to investigate the diversity and response of HCB associated with a cosmopolitan marine diatom, Skeletonema costatum, to crude oil. Pyrosequencing analysis and qPCR revealed a dramatic transition in the diatom-associated bacterial community, defined initially by a short-lived bloom of Methylophaga (putative oil degraders) that was subsequently succeeded by distinct groups of HCB (Marinobacter, Polycyclovorans, Arenibacter, Parvibaculum, Roseobacter clade), including putative novel phyla, as well as other groups with previously unqualified oil-degrading potential. Interestingly, these oil-enriched organisms contributed to the apparent and exclusive biodegradation of substituted and non-substituted polycyclic aromatic hydrocarbons (PAHs), thereby suggesting that the HCB community associated with the diatom is tuned to specializing in the degradation of PAHs. Furthermore, the formation of marine oil snow (MOS) in oil-amended incubations was consistent with its formation during the Deepwater Horizon oil spill. This work highlights the phycosphere of phytoplankton as an underexplored biotope in the ocean where HCB may contribute importantly to the biodegradation of hydrocarbon contaminants in marine surface waters.

  • Intestinal microbiota signatures associated with inflammation history in mice experiencing recurring colitis

    Berry D, Kuzyk O, Rauch I, Heider S, Schwab C, Hainzl E, Decker T, Müller M, Strobl B, Schleper C, Urich T, Wagner M, Kenner L, Loy A
    2015 - Front Microbiol, 6: 1408

    Abstract: 

    Acute colitis causes alterations in the intestinal microbiota, but the microbiota is thought to recover after such events. Extreme microbiota alterations are characteristic of human chronic inflammatory bowel diseases, although alterations reported in different studies are divergent and sometimes even contradictory. To better understand the impact of periodic disturbances on the intestinal microbiota and its compositional difference between acute and relapsing colitis, we investigated the beginnings of recurrent inflammation using the dextran sodium sulfate (DSS) mouse model of chemically induced colitis. Using bacterial 16S rRNA gene-targeted pyrosequencing as well as quantitative fluorescence in situ hybridization, we profiled the intestinal and stool microbiota of mice over the course of three rounds of DSS-induced colitis and recovery. We found that characteristic inflammation-associated microbiota could be detected in recovery-phase mice. Successive inflammation episodes further drove the microbiota into an increasingly altered composition post-inflammation, and signatures of colitis history were detectable in the microbiota more sensitively than by pathology analysis. Bacterial indicators of murine colitis history were identified in intestinal and stool samples, with a high degree of consistency between both sample types. Stool may therefore be a promising non-invasive source of bacterial biomarkers that are highly sensitive to inflammation state and history.

  • Intestinal epithelial cell tyrosine kinase 2 transduces interleukin-22 signals to protect from acute colitis

    Hainzl E, Stockinger S, Rauch I, Heider S, Berry D, Lassnig C, Schwab C, Rosebrock F, Milinovich G, Schlederer M, Wagner M, Schleper C, Loy A, Urich T, Kenner L, Han X, Decker T, Strobl B, Müller M
    2015 - J Immunol., 195: 5011-5024

    Abstract: 

    In the intestinal tract, IL-22 activates signal transducer and activator of transcription 3 (STAT3) to promote intestinal epithelial cell (IEC) homeostasis and tissue healing. The mechanism has remained obscure but we demonstrate that IL-22 acts via tyrosine kinase 2 (Tyk2), a member of the Janus kinase (Jak) family. Using a mouse model for colitis, we show that Tyk2 deficiency is associated with an altered composition of the gut microbiota and exacerbates inflammatory bowel disease (IBD). Colitic Tyk2-/- mice have less phosphorylated STAT3 (pY-STAT3) in colon tissue and their IECs proliferate less efficiently. Tyk2-deficient primary IECs show reduced pY-STAT3 in response to IL-22 stimulation and expression of IL-22-STAT3 target genes is reduced in IECs from healthy and colitic Tyk2-/- mice. Experiments with conditional Tyk2-/- mice reveal that IEC-specific depletion of Tyk2 aggravates colitis. Disease symptoms can be alleviated by administering high doses of recombinant IL-22-Fc, indicating that Tyk2 deficiency can be rescued via the IL-22 receptor complex. The pivotal function of Tyk2 in IL-22-dependent colitis was confirmed in Citrobacter rodentium-induced disease. Thus, Tyk2 protects against acute colitis in part by amplifying inflammation-induced epithelial IL-22 signaling to STAT3. 

  • A flexible and economical barcoding approach for highly multiplexed amplicon sequencing of diverse target genes.

    Herbold CW, Pelikan C, Kuzyk O, Hausmann B, Angel R, Berry D, Loy A
    2015 - Front Microbiol, 6: 731

    Abstract: 

    High throughput sequencing of phylogenetic and functional gene amplicons provides tremendous insight into the structure and functional potential of complex microbial communities. Here, we introduce a highly adaptable and economical PCR approach to barcoding and pooling libraries of numerous target genes. In this approach, we replace gene- and sequencing platform-specific fusion primers with general, interchangeable barcoding primers, enabling nearly limitless customized barcode-primer combinations. Compared to barcoding with long fusion primers, our multiple-target gene approach is more economical because it overall requires lower number of primers and is based on short primers with generally lower synthesis and purification costs. To highlight our approach, we pooled over 900 different small-subunit rRNA and functional gene amplicon libraries obtained from various environmental or host-associated microbial community samples into a single, paired-end Illumina MiSeq run. Although the amplicon regions ranged in size from approximately 290 to 720 bp, we found no significant systematic sequencing bias related to amplicon length or gene target. Our results indicate that this flexible multiplexing approach produces large, diverse, and high quality sets of amplicon sequence data for modern studies in microbial ecology.

  • Cyanate as an energy source for nitrifiers.

    Palatinszky M, Herbold C, Jehmlich N, Pogoda M, Han P, von Bergen M, Lagkouvardos I, Karst SM, Galushko A, Koch H, Berry D, Daims H, Wagner M
    2015 - Nature, 524: 105-108

    Abstract: 

    Ammonia- and nitrite-oxidizing microorganisms are collectively responsible for the aerobic oxidation of ammonia via nitrite to nitrate and have essential roles in the global biogeochemical nitrogen cycle. The physiology of nitrifiers has been intensively studied, and urea and ammonia are the only recognized energy sources that promote the aerobic growth of ammonia-oxidizing bacteria and archaea. Here we report the aerobic growth of a pure culture of the ammonia-oxidizing thaumarchaeote Nitrososphaera gargensis using cyanate as the sole source of energy and reductant; to our knowledge, the first organism known to do so. Cyanate, a potentially important source of reduced nitrogen in aquatic and terrestrial ecosystems, is converted to ammonium and carbon dioxide in Nitrososphaera gargensis by a cyanase enzyme that is induced upon addition of this compound. Within the cyanase gene family, this cyanase is a member of a distinct clade also containing cyanases of nitrite-oxidizing bacteria of the genus Nitrospira. We demonstrate by co-culture experiments that these nitrite oxidizers supply cyanase-lacking ammonia oxidizers with ammonium from cyanate, which is fully nitrified by this microbial consortium through reciprocal feeding. By screening a comprehensive set of more than 3,000 publically available metagenomes from environmental samples, we reveal that cyanase-encoding genes clustering with the cyanases of these nitrifiers are widespread in the environment. Our results demonstrate an unexpected metabolic versatility of nitrifying microorganisms, and suggest a previously unrecognized importance of cyanate in cycling of nitrogen compounds in the environment.

  • Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells

    Berry D, Mader E, Lee TK, Woebken D, Wang Y, Zhu D, Palatinszky M, Schintlmeister A, Schmid MC, Hanson BT, Shterzer N, Mizrahi I, Rauch I, Decker T, Bocklitz T, Popp J, Gibson CM, Fowler PW, Huang WE, Wagner M
    2015 - Proc Natl Acad Sci USA, 112: E194-203

    Abstract: 

    Microbial communities are essential to the function of virtually all ecosystems and eukaryotes, including humans. However, it is still a major challenge to identify microbial cells active under natural conditions in complex systems. In this study, we developed a new method to identify and sort active microbes on the single-cell level in complex samples using stable isotope probing with heavy water (D2O) combined with Raman microspectroscopy. Incorporation of D2O-derived D into the biomass of autotrophic and heterotrophic bacteria and archaea could be unambiguously detected via C-D signature peaks in single-cell Raman spectra, and the obtained labeling pattern was confirmed by nanoscale-resolution secondary ion MS. In fast-growing Escherichia coli cells, label detection was already possible after 20 min. For functional analyses of microbial communities, the detection of D incorporation from D2O in individual microbial cells via Raman microspectroscopy can be directly combined with FISH for the identification of active microbes. Applying this approach to mouse cecal microbiota revealed that the host-compound foragers Akkermansia muciniphila and Bacteroides acidifaciens exhibited distinctive response patterns to amendments of mucin and sugars. By Raman-based cell sortingof active (deuterated) cells with optical tweezers and subsequent multiple displacement amplification and DNA sequencing, novel cecal microbes stimulated by mucin and/or glucosamine were identified, demonstrating the potential of the nondestructive D2O-Raman approach for targeted sortingof microbial cells with defined functional properties for single-cell genomics.

  • Type I interferons have opposing effects during the emergence and recovery phases of colitis.

    Rauch I, Hainzl E, Rosebrock F, Heider S, Schwab C, Berry D, Stoiber D, Wagner M, Schleper C, Loy A, Urich T, Müller M, Strobl B, Kenner L, Decker T
    2014 - Eur J Immunol., 44: 2749-60

    Abstract: 

    The contribution of the innate immune system to inflammatory bowel disease (IBD) is under intensive investigation. Research in animal models has demonstrated that type I interferons (IFN-Is) protect from IBD. In contrast, studies of patients with IBD have produced conflicting results concerning the therapeutic potential of IFN-Is. Here, we present data suggesting that IFN-Is play dual roles as regulators of intestinal inflammation in dextran sodium sulfate (DSS)-treated C57BL/6 mice. Though IFN-Is reduced acute intestinal damage and the abundance ofcolitis-associated intestinal bacteria caused by treatment with a high dose of DSS, they also inhibited the resolution of inflammation after DSS treatment. IFN-Is played an anti-inflammatory role by suppressing the release of IL-1β from the colon MHC class II(+) cells. Consistently, IL-1 receptor blockade reduced the severity of inflammation in IFN-I receptor-deficient mice and myeloid cell-restricted ablation of the IFN-I receptor was detrimental. The proinflammatory role of IFN-Is during recovery from DSS treatment was caused by IFN-I-dependent cell apoptosis as well as an increase in chemokine production and infiltrating inflammatory monocytes and neutrophils. Thus, IFN-Is play opposing roles in specificphases of intestinal injury and inflammation, which may be important for guiding treatment strategies in patients.

  • Deciphering microbial interactions and detecting keystone species with co-occurrence networks

    Berry D, Widder S
    2014 - Front Microbiol, 5: 219

    Abstract: 

    Co-occurrence networks produced from microbial survey sequencing data are frequently used to identify interactions between community members. While this approach has potential to reveal ecological processes, it has been insufficiently validated due to the technical limitations inherent in studying complex microbial ecosystems. Here, we simulate multi-species microbial communities with known interaction patterns using generalized Lotka-Volterra dynamics. We then construct co-occurrence networks and evaluate how well networks reveal the underlying interactions and how experimental and ecological parameters can affect network inference and interpretation. We find that co-occurrence networks can recapitulate interaction networks under certain conditions, but that they lose interpretability when the effects of habitat filtering become significant. We demonstrate that networks suffer from local hot spots of spurious correlation in the neighborhood of hub species that engage in many interactions. We also identify topological features associated with keystone species in co-occurrence networks. This study provides a substantiated framework to guide environmental microbiologists in the construction and interpretation of co-occurrence networks from microbial survey datasets.

  • High-fat diet alters gut microbiota physiology in mice

    Daniel H, Moghaddas Gholami A, Berry D, Desmarchelier C, Hahne H, Loh G, Mondot S, Lepage P, Rothballer M, Walker A, Böhm C, Wenning M, Wagner M, Blaut M, Schmitt-Kopplin P, Kuster B, Haller D, Clavel T
    2014 - ISME J., 8: 295-308

    Abstract: 

    The intestinal microbiota is known to regulate host energy homeostasis and can be influenced by high-calorie diets. However, changes affecting the ecosystem at the functional level are still not well characterized. We measured shifts in cecal bacterial communities in mice fed a carbohydrate orhigh-fat (HF) diet for 12 weeks at the level of the following: (i) diversity and taxa distribution by high-throughput 16S ribosomal RNA gene sequencing; (ii) bulk and single-cell chemical composition by Fourier-transform infrared- (FT-IR) and Raman micro-spectroscopy and (iii) metaproteome and metabolome via high-resolution mass spectrometry. High-fat diet caused shifts in the diversity of dominant gut bacteria and altered the proportion of Ruminococcaceae (decrease) and Rikenellaceae (increase). FT-IR spectroscopy revealed that the impact of the diet on cecal chemical fingerprints is greater than the impact of microbiota composition. Diet-driven changes in biochemical fingerprints of members of the Bacteroidales and Lachnospiraceae were also observed at the level of single cells, indicating that there were distinct differences in cellular composition of dominant phylotypes under different diets. Metaproteome and metabolome analyses based on the occurrence of 1760 bacterial proteins and 86 annotated metabolites revealed distinct HF diet-specific profiles. Alteration of hormonal and anti-microbial networks, bile acid and bilirubin metabolism and shifts towards amino acid and simple sugars metabolism were observed. We conclude that a HF diet markedly affects the gut bacterial ecosystem at the functional level.

  • Removal of pharmaceuticals and personal care products during water recycling: microbial community structure and effects of substrate concentration.

    Oneisis-Barry K, Berry D, Proscher J, Sivakumar IKA, Bouwer E
    2014 - Appl Environ Microbiol., 80: 2440-50

    Abstract: 

    Many pharmaceuticals and personal care products (PPCPs) have been shown to be biotransformed in water treatment systems. However, little research exists on the effect of initial PPCP concentration on PPCP biotransformation or on the microbial communities treating impacted water. In this study, biological PPCP removal at various concentrations was assessed using laboratory columns inoculated with wastewater treatment plant effluent. Pyrosequencing was used to examine microbial communities in the columns and in soil from a soil aquifer treatment (SAT; a method ofwater treatment prior to reuse) site. Laboratory columns were supplied with different concentrations (0.25, 10, 100, or 1,000 μg liter(-1)) of each of 15 PPCPs. Five PPCPs (4-isopropyl-3-methylphenol [biosol], p-chloro-m-xylenol, gemfibrozil, ketoprofen, and phenytoin) were not removed at any tested concentrations. Two PPCPs (naproxen and triclosan) exhibited removals independent of PPCP concentration. PPCP removal efficiencies were dependent on initial concentrations for biphenylol, p-chloro-m-cresol, chlorophene, diclofenac, 5-fluorouracil, ibuprofen, and valproic acid, showing that PPCP concentration can affect biotransformation. Biofilms from sand samples collected from the 0.25- and 10-μg liter(-1) PPCP columns were pyrosequenced along with SAT soil samples collected on three consecutive days of a wetting and drying cycle to enable comparison of these two communities exposed to PPCPs. SAT communities were similar to column communities in taxonomy and phylotype composition, and both were found to contain close relatives of known PPCP degraders. The efficiency of biological removal of PPCPs was found to be dependent on the concentration at which the contamination occurs for some, but not all, PPCPs.

  • Longitudinal study of murine microbiota activity and interactions with the host during acute inflammation and recovery

    Schwab C, Berry D, Rauch I, Rennisch I, Ramesmayer J, Hainzl E, Heider S, Decker T, Kenner L, Müller M, Strobl B, Wagner M, Schleper C, Loy A, Urich T
    2014 - ISME J., 8(5):1101-14

    Abstract: 

    Although alterations in gut microbiota composition during acute colitis have been repeatedly observed, associated functional changes and therecovery from dysbiosis received little attention. In this study, we investigated structure and function of the gut microbiota during acute inflammationand recovery in a dextran sodium sulfate (DSS)-colitis mouse model using metatranscriptomics, bacterial 16S rRNA gene amplicon sequencing and monitoring of selected host markers. Parallel to an increase of host markers of inflammation during acute colitis, we observed relative abundance shifts and alterations in phylotype composition of the dominant bacterial orders Clostridiales and Bacteroidales, and an increase of the low abundant Enterobacteriales, Deferribacterales, Verrucomicrobiales and Erysipelotrichales. During recovery, the microbiota began to resume, but did not reach its original composition until the end of the experiment. Microbial gene expression was more resilient to disturbance, with pre-perturbation-type transcript profiles appearing quickly after acute colitis. The decrease of Clostridiales during inflammation correlated with a reduction of transcripts related to butyrate formation, suggesting a disturbance in host-microbe signalling and mucosal nutrient provision. The impact of acute inflammationon the Clostridiales was also characterized by a significant downregulation of their flagellin-encoding genes. In contrast, the abundance of members of the Bacteroidales increased along with an increase in transcripts related to mucin degradation. We propose that acute inflammation triggered a selective reaction of the immune system against flagella of commensals and temporarily altered murine microbiota composition and functions relevant for the host. Despite changes in specific interactions, the host-microbiota homeostasis revealed a remarkable ability for recovery.

  • Intestinal microbiota reduces genotoxic endpoints induced by high-energy protons.

    Maier I, Berry D, Schiesl R
    2014 - Radiat Res, 181: 45-53

    Abstract: 

    Ionizing space radiation causes oxidative DNA damage and triggers oxidative stress responses, and compromised DNA repair mechanisms can lead to increased risk of carcinogenesis. Young adult mice with developed innate and adaptive immune systems that harbored either a conventionalintestinal microbiota (CM) or an intestinal microbiota with a restricted microbial composition (RM) were irradiated with a total dose of 1 Gy delivered by high-energy protons (2.5 GeV/n, LET = 0.2-2 keV/μm) or silicon or iron ions (850 MeV/n, LET ≈ 50 keV/μm and 1 GeV/n, LET = 150 keV/μm, respectively). Six hours after whole-body irradiation, acute chromosomal DNA lesions were observed for RM mice but not CM mice. High-throughput rRNA gene sequencing of intestinal mucosal bacteria showed that Barnesiella intestinihominis and unclassified Bacterodiales were significantly more abundant in male RM mice than CM mice, and phylotype densities changed in irradiated mice. In addition, Helicobacter hepaticus and Bacteroides stercoris were higher in CM than RM mice. Elevated levels of persistently phosphorylated γ-H2AX were observed in RM mice exposed to high-energy protons compared to nonirradiated RM mice, and they also were associated with a decrease of the antioxidant glutathione in peripheral blood measured at four weeks after irradiation. After radiation exposure, CM mice showed lower levels of γ-H2AX phosphorylation than RM mice and an increase in specific RM-associated phylotypes, indicating a down-regulating force on DNA repair by differentially abundant phylotypes in RM versus a radiation-sensitive complex CM.

  • Endospores of thermophilic bacteria as tracers of microbial dispersal by ocean currents

    Müller A, de Rezende JR, Hubert C, Kjeldsen KU, Lagkouvardos I, Berry D, Jørgensen BB, Loy A
    2014 - ISME J., 8: 1153-65

    Abstract: 

    Microbial biogeography is influenced by the combined effects of passive dispersal and environmental selection, but the contribution of either factor can be difficult to discern. As thermophilic bacteria cannot grow in the cold seabed, their inactive spores are not subject to environmental selection. We therefore conducted a global experimental survey using thermophilic endospores that are passively deposited by sedimentation to the cold seafloor as tracers to study the effect of dispersal by ocean currents on the biogeography of marine microorganisms. Our analysis of 81 different marine sediments from around the world identified 146 species-level 16S rRNA phylotypes of endospore-forming, thermophilic Firmicutes. Phylotypes showed various patterns of spatial distribution in the world oceans and were dispersal-limited to different degrees. Co-occurrence of several phylotypes in locations separated by great distances (west of Svalbard, the Baltic Sea and the Gulf of California) demonstrated a widespread but not ubiquitous distribution. In contrast, Arctic regions with water masses that are relatively isolated from global ocean circulation (Baffin Bay and east of Svalbard) were characterized by low phylotype richness and different compositions of phylotypes. The observed distribution pattern ofthermophilic endospores in marine sediments suggests that the impact of passive dispersal on marine microbial biogeography is controlled by the connectivity of local water masses to ocean circulation.

  • Polycyclic aromatic hydrocarbon degradation of a phytoplankton-associated Arenibacter spp. and description of Arenibacter algicola sp. nov., an aromatic hydrocarbon-degrading bacterium

    Gutierrez T, Rhodes G, Mishamandani S, Berry D, Whitman WB, Nichols PD, Semple KT, Aitken MD
    2014 - Appl Environ Microbiol., 80: 618-28

    Abstract: 

    Pyrosequencing of the bacterial community associated with a cosmopolitan marine diatom during enrichment with crude oil revealed severalArenibacter phylotypes, of which one (OTU-202) had become significantly enriched by the oil. Since members of the genus Arenibacter have not been previously shown to degrade hydrocarbons, we attempted to isolate a representative strain of this genus in order to directly investigate itshydrocarbon-degrading potential. Based on 16S rRNA sequencing, one isolate (designated strain TG409(T)) exhibited >99% sequence identity to three type strains of this genus. On the basis of phenotypic and genotypic characteristics, strain TG409(T) represents a novel species in the genusArenibacter, for which the name Arenibacter algicola sp. nov. is proposed. We reveal for the first time that polycyclic aromatic hydrocarbon (PAH)degradation is a shared phenotype among members of this genus, indicating that it could be used as a taxonomic marker for this genus. Kinetic data for PAH mineralization rates showed that naphthalene was preferred to phenanthrene, and its mineralization was significantly enhanced in the presence of glass wool (a surrogate for diatom cell surfaces). During enrichment on hydrocarbons, strain TG409(T) emulsified n-tetradecane and crude oil, and cells were found to be preferentially attached to oil droplets, indicating an ability by the strain to express cell surface amphiphilic substances (biosurfactants or bioemulsifiers) as a possible strategy to increase the bioavailability of hydrocarbons. This work adds to our growing knowledge on the diversity of bacterial genera in the ocean contributing to the degradation of oil contaminants and of hydrocarbon-degrading bacteria found living in association with marine eukaryotic phytoplankton.

  • NxrB encoding the beta subunit of nitrite oxidoreductase as functional and phylogenetic marker for nitrite-oxidizing Nitrospira

    Pester M, Maixner F, Berry D, Rattei T, Koch H, Lücker S, Nowka B, Richter A, Spieck E, Lebedeva E, Loy A, Wagner M, Daims H
    2014 - Environ Microbiol, 16: 3055-3071

    Abstract: 

    Nitrospira are the most widespread and diverse known nitrite-oxidizing bacteria and key nitrifiers in natural and engineered ecosystems. Nevertheless, their ecophysiology and environmental distribution are understudied due to the recalcitrance of Nitrospira to cultivation and the lack of a molecular functional marker, which would allow the detection of Nitrospira in the environment. Here we introduce nxrB, the gene encoding subunit beta of nitrite oxidoreductase, as a functional and phylogenetic marker for Nitrospira. Phylogenetic trees based on nxrB of Nitrospira were largely congruent to 16S rRNA-based phylogenies. By using new nxrB-selective PCR primers, we obtained almost full-length nxrB sequences from Nitrospira cultures, two activated sludge samples, and several geographically and climatically distinct soils. Amplicon pyrosequencing of nxrB fragments from 16 soils revealed a previously unrecognized diversity of terrestrial Nitrospira with 1,801 detected species-level OTUs (using an inferred species threshold of 95% nxrB identity). Richness estimates ranged from 10 to 946 co-existing Nitrospira species per soil. Comparison to an archaeal amoA dataset obtained from the same soils [Environ. Microbiol. 14: 525-539 (2012)] uncovered that ammonia-oxidizing archaea and Nitrospira communities were highly correlated across the soil samples, possibly indicating shared habitat preferences or specific biological interactions among members of these nitrifier groups.

  • Polycyclic Aromatic Hydrocarbon Degradation of Phytoplankton-Associated Arenibacter spp. and Description of Arenibacter algicola sp. nov., an Aromatic Hydrocarbon-Degrading Bacterium

    Gutierrez T, Rhodes G, Mishamandani S, Berry D, Whitman WB, Nichols PD, Semple KT, Aitken MD
    2013 - Appl. Environ. Microbiol., 80:2 618-628
  • Temporal bacterial community dynamics vary among ulcerative colitis patients after fecal microbiota transplantation

    Angelberger S, Reinisch W, Makristathis A, Lichtenberger C, Dejaco C, Papay P, Novacek G, Trauner M, Loy A, Berry D
    2013 - Am. J. Gastroenterol., 108: 1620-1630

    Abstract: 

    OBJECTIVES: Fecal microbiota transplantation (FMT) from healthy donors, which is an effective alternative for treatment of Clostridium difficile-associated disease, is being considered for several disorders such as inflammatory bowel disease, irritable bowel syndrome, and metabolic syndrome. Disease remission upon FMT is thought to be facilitated by an efficient colonization of healthy donor microbiota, but knowledge of the composition and temporal stability of patient microbiota after FMT is lacking.METHODS:Five patients with moderately to severely active ulcerative colitis (Mayo score ≥6) and refractory to standard therapy received FMT via nasojejunal tube and enema. In addition to clinical activity and adverse events, the patients' fecal bacterial communities were monitored at multiple time points for up to 12 weeks using 16S rRNA gene-targeted pyrosequencing. RESULTS:FMT elicited fever and a temporary increase of C-reactive protein. Abundant bacteria from donors established in recipients, but the efficiency and stability of donor microbiota colonization varied greatly. A positive clinical response was observed after 12 weeks in one patient whose microbiota had been effectively augmented by FMT. This augmentation was marked by successive colonization of donor-derived phylotypes including the anti-inflammatory and/or short-chain fatty acid-producing Faecalibacterium prausnitzii, Rosebura faecis, and Bacteroides ovatus. Disease severity (as measured by the Mayo score) was associated with an overrepresentation of Enterobacteriaceae and an underrepresentation of Lachnospiraceae. CONCLUSIONS:This study highlights the value of characterizing temporally resolved microbiota dynamics for a better understanding of FMT efficacy and provides potentially useful diagnostic indicators for monitoring FMT success in the treatment of ulcerative colitis.

  • Intestinal bacteria modify lymphoma incidence and latency by affecting systemic inflammatory state, oxidative stress, and leucocyte genotoxicity

    Yamamoto ML, Maier I, Dang AT, Berry D, Liu J, Ruegger PM, Yang J, Soto PA, Presley LL, Reliene R, Westbrook AM, Wei B, Loy A, Chang C, Braun J, Borneman J, Schiestl RH
    2013 - Cancer Res., 73: 4222-4232

    Abstract: 

    Ataxia-telangiectasia is a genetic disorder associated with high incidence of B-cell lymphoma. Using an ataxia-telangiectasia mouse model, we compared lymphoma incidence in several isogenic mouse colonies harboring different bacterial communities, finding that intestinal microbiota are a major contributor to disease penetrance and latency, lifespan, molecular oxidative stress, and systemic leukocyte genotoxicity. High-throughput sequence analysis of rRNA genes identified mucosa-associated bacterial phylotypes that were colony-specific. Lactobacillus johnsonii, which was deficient in the more cancer-prone mouse colony, was causally tested for its capacity to confer reduced genotoxicity when restored by short-term oral transfer. This intervention decreased systemic genotoxicity, a response associated with reduced basal leukocytes and the cytokine-mediated inflammatory state, and mechanistically linked to the host cell biology of systemic genotoxicity. Our results suggest that intestinal microbiota are a potentially modifiable trait for translational intervention in individuals at risk for B-cell lymphoma, or for other diseases that are driven by genotoxicity or the molecular response to oxidative stress.

  • Role of bacterial exopolymers (EPS) in the fate of the oil released during the Deepwater Horizon oil spill

    Gutierrez T, Berry D, Yang T, Mishamandani S, McKay L, Teske A, Aitken MD
    2013 - PLoS One, 8(6):e67717

    Abstract: 

    Halomonas species are recognized for producing exopolysaccharides (EPS) exhibiting amphiphilic properties that allow these macromolecules to interface with hydrophobic substrates, such as hydrocarbons. There remains a paucity of knowledge, however, on the potential of Halomonas EPS to influence the biodegradation of hydrocarbons. In this study, the well-characterized amphiphilic EPS produced by Halomonas species strain TG39 was shown to effectively increase the solubilization of aromatic hydrocarbons and enhance their biodegradation by an indigenous microbial community from oil-contaminated surface waters collected during the active phase of the Deepwater Horizon oil spill. Three Halomonas strains were isolated from the Deepwater Horizon site, all of which produced EPS with excellent emulsifying qualities and shared high (97-100%) 16S rRNA sequence identity with strain TG39 and other EPS-producing Halomonas strains. Analysis of pyrosequence data from surface water samples collected during the spill revealed several distinct Halomonas phylotypes, of which some shared a high sequence identity (≥97%) to the Halomonas isolates. Other bacterial groups comprising members with well-characterized EPS-producing qualities, such as Alteromonas, Colwellia and Pseudoalteromonas, were also found enriched in surface waters, suggesting that the total pool of EPS in the Gulf during the spill may have been supplemented by these organisms. Roller bottle incubations with one of the Halomonas isolates from Deepwater Horizon demonstrated its ability to effectively produce oil aggregates and emulsify the oil. The enrichment of EPS-producing bacteria during the spill coupled with their capacity to produce amphiphilic EPS is likely to have contributed to the ultimate removal of the oil and in the formation of oil aggregates, which were a dominant feature observed in contaminated surface waters.

  • Colonization resistance and microbial ecophysiology: Using gnotobiotic mouse models and single-cell technology to explore the intestinal jungle

    Stecher B, Berry D, Loy A
    2013 - FEMS Microbiol. Rev., 37: 793-829

    Abstract: 

    The highly diverse intestinal microbiota forms a structured community engaged in constant communication with itself and its host and is characterized by extensive ecological interactions. A key benefit that the microbiota affords its host is its ability to protect against infections in a process termed colonization resistance (CR), which remains insufficiently understood. In this review, we connect basic concepts of CR with new insights from recent years and highlight key technological advances in the field of microbial ecology. We present a selection of statistical and bioinformatics tools used to generate hypotheses about synergistic and antagonistic interactions in microbial ecosystems from metagenomic datasets. We emphasize the importance of experimentally testing these hypotheses and discuss the value of gnotobiotic mouse models for investigating specific aspects related to microbiota-host-pathogen interactions in a well-defined experimental system. We further introduce new developments in the area of single-cell analysis using fluorescence in situ hybridization in combination with metabolic stable isotope labeling technologies for studying the in vivo activities of complex community members. These approaches promise to yield novel insights into the mechanisms of CR and intestinal ecophysiology in general, and give researchers the means to experimentally test hypotheses in vivo at varying levels of biological and ecological complexity.

  • Host-compound foraging by intestinal microbiota revealed by single-cell stable isotope probing

    Berry D, Stecher B, Schintlmeister A, Reichert J, Brugiroux S, Wildd B, Wanek W, Richter A, Rauch I, Decker T, Loy A, Wagner M
    2013 - Proc. Natl. Acad. Sci. USA, 110: 4720-4725

    Abstract: 

    The animal and human intestinal mucosa secretes an assortment of compounds to establish a physical barrier between the host tissue and intestinal contents, a separation that is vital for health. Some pathogenic microorganisms as well as members of the commensal intestinal microbiota have been shown to be able to break down these secreted compounds. Our understanding of host-compound degradation by the commensal microbiota has been limited to knowledge about simplified model systems because of the difficulty in studying the complex intestinal ecosystem in vivo. In this study, we introduce an approach that overcomes previous technical limitations and allows us to observe which microbial cells in the intestine use host-derived compounds. We added stable isotope-labeled threonine i.v. to mice and combined fluorescence in situ hybridization with high-resolution secondary ion mass spectrometry imaging to characterize utilization of host proteins by individual bacterial cells. We show that two bacterial species, Bacteroides acidifaciens and Akkermansia muciniphila, are important host-protein foragers in vivo. Using gnotobiotic mice we show that microbiota composition determines the magnitude and pattern of foraging by these organisms, demonstrating that a complex microbiota is necessary in order for this niche to be fully exploited. These results underscore the importance of in vivo studies of intestinal microbiota, and the approach presented in this study will be a powerful tool to address many other key questions in animal and human microbiome research.

  • Intestinal microbiota: a source of novel biomarkers in inflammatory bowel disease?

    Berry D, Reinisch W
    2013 - Best Pract. Res. Clin. Gastroenterol., 27: 47-58

    Abstract: 

    The human intestine harbors a complex microbial ecosystem that performs manifold functions important to the nutrition and health of its host. Extensive study has revealed that the composition of the intestinal microbiota is altered in individuals with inflammatory bowel disease (IBD). The IBD associated intestinal microbiota generally has reduced species richness and diversity, lower temporal stability, and disruption of the secreted mucus layer structure. Multiple studies have identified certain bacterial taxa that are enriched or depleted in IBD including Enterobacteriaceae, Ruminococcus gnavus, and Desulfovibrio (enriched) and Faecalibacterium prausnitzii, Lachnospiraceae, and Akkermansia (depleted). Additionally, the relative abundance of some taxa appears to correlate with established markers of disease activity such as Enterobacteriaceae (enriched) and Lachnospiraceae (depleted). Signature shifts in fecal microbial community composition may therefore prove to be valuable as diagnostic biomarkers, particularly for longitudinal monitoring of disease activity and response to treatments.

  • Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and stable isotope probing

    Gutierrez T, Berry D, Yang T, Mishamandani S, McKay L, Teske A, Aitken MD
    2013 - ISME J., 7: 2091-2104

    Abstract: 

    The massive influx of crude oil into the Gulf of Mexico during the Deepwater Horizon disaster triggered dramatic microbial community shifts in surface oil slick and deep plume waters. Previous work had shown several taxa, notably DWH Oceanospirillales, Cycloclasticus and Colwellia, were found enriched in these waters based on their dominance in conventional clone and pyrosequencing libraries and were thought to have played a significant role in the degradation of the oil. However, this type of community analysis data failed to provide direct evidence on the functional properties, such as hydrocarbon degradation of organisms. Using DNA-based stable-isotope probing (DNA-SIP) with uniformly 13C-labelled hydrocarbons, we identified several aliphatic- (Alcanivorax, Marinobacter) and polycyclic aromatic hydrocarbon (PAH)- (Alteromonas, Cycloclasticus, Colwellia) degrading bacteria. We also isolated several strains (Alcanivorax, Alteromonas, Cycloclasticus, Halomonas, Marinobacter and Pseudoalteromonas) with demonstrable hydrocarbon-degrading qualities from surface slick and plume water samples collected during the active phase of the spill. Some of these organisms accounted for the majority of sequence reads representing their respective taxa in a pyrosequencing dataset constructed from the same and additional water column samples. Hitherto, Alcanivorax was not identified in any of the previous water column studies analysing the microbial response to the spill and we discuss its failure to respond to the oil. Collectively, our data provides unequivocal evidence on the hydrocarbon-degrading qualities for some of the dominant taxa enriched in surface and plume waters during the Deepwater Horizon oil spill, and a more complete understanding of their role in the fate of the oil.

  • A dynamic and complex monochloramine stress response in Escherichia coli revealed by transcriptome analysis

    Holder D, Berry D, Dai D, Raskin L, Xi C
    2013 - Water Res., 47: 4978-4985

    Abstract: 

    Despite the widespread use of monochloramine in drinking water treatment, there is surprisingly little information about its mode of action. In this study, DNA microarrays were used to investigate the global gene expression of Escherichia coli cells exposed to sub-lethal concentrations of monochloramine, with a focus on temporal dynamics. Genes induced by monochloramine were associated with several stress response functions, including oxidative stress, DNA repair, multidrug efflux, biofilm formation, antibiotic resistance, and cell wall repair. The diversity of functional associations supports a model of monochloramine action involving multiple cellular targets including cell membranes, nucleic acids, and proteins. These data suggest that E. coli responds to monochloramine exposure by activating diverse defense responses rather than a single antioxidant system and the exposure may also induce biofilm formation. The induction of multidrug efflux pumps and specific antibiotic resistance genes further suggests that exposure to monochloramine may contribute to reduced susceptibility to some antibiotics.

  • Phylotype-level 16S rRNA analysis reveals new bacterial indicators of health state in acute murine colitis

    Berry D, Schwab C, Milinovich G, Reichert J, Ben Mahfoudh K, Decker T, Engel M, Hai B, Hainzl E, Heider S, Kenner L, Müller M, Rauch I, Strobl B, Wagner M, Schleper C, Urich T, Loy A
    2012 - ISME J., 6: 2091-106

    Abstract: 

    Human inflammatory bowel disease and experimental colitis models in mice are associated with shifts in intestinal microbiota composition, but it is unclear at what taxonomic/phylogenetic level such microbiota dynamics can be indicative for health or disease. Here, we report that dextran sodium sulfate (DSS)-induced colitis is accompanied by major shifts in the composition and function of the intestinal microbiota of STAT1(-/-) and wild-type mice, as determined by 454 pyrosequencing of bacterial 16S rRNA (gene) amplicons, metatranscriptomics and quantitative fluorescence in situ hybridization of selected phylotypes. The bacterial families Ruminococcaceae, Bacteroidaceae, Enterobacteriaceae, Deferribacteraceae and Verrucomicrobiaceae increased in relative abundance in DSS-treated mice. Comparative 16S rRNA sequence analysis at maximum possible phylogenetic resolution identified several indicator phylotypes for DSS treatment, including the putative mucin degraders Akkermansia and Mucispirillum. The analysis additionally revealed strongly contrasting abundance changes among phylotypes of the same family, particularly within the Lachnospiraceae. These extensive phylotype-level dynamics were hidden when reads were grouped at higher taxonomic levels. Metatranscriptomic analysis provided insights into functional shifts in the murine intestinal microbiota, with increased transcription of genes associated with regulation and cell signaling, carbohydrate metabolism and respiration and decreased transcription of flagellin genes during inflammation. These findings (i) establish the first in-depth inventory of the mouse gut microbiota and its metatranscriptome in the DSS colitis model, (ii) reveal that family-level microbial community analyses are insufficient to reveal important colitis-associated microbiota shifts and (iii) support a scenario of shifting intra-family structure and function in the phylotype-rich and phylogenetically diverse Lachnospiraceae in DSS-treated mice.

  • Barcoded primers used in multiplex amplicon pyrosequencing bias amplification

    Berry D, Ben Mahfoudh K, Wagner M, Loy A
    2011 - Appl. Environ. Microbiol., 77: 7846-7849

    Abstract: 

    "Barcode-tagged" PCR primers used for multiplex amplicon sequencing generate a thus-far-overlooked amplification bias that produces variable terminal restriction fragment length polymorphism (T-RFLP) and pyrosequencing data from the same environmental DNA template. We propose a simple two-step PCR approach that increases reproducibility and consistently recovers higher genetic diversity in pyrosequencing libraries.

  • Systematic spatial bias in DNA microarray hybridization is caused by probe spot position-dependent variability in lateral diffusion

    Steger D, Berry D, Haider S, Horn M, Wagner M, Stocker R, Loy A
    2011 - PLoS One, 6: e23727

    Abstract: 

    The hybridization of nucleic acid targets with surface-immobilized probes is a widely used assay for the parallel detection of multiple targets in medical and biological research. Despite its widespread application, DNA microarray technology still suffers from several biases and lack of reproducibility, stemming in part from an incomplete understanding of the processes governing surface hybridization. In particular, non-random spatial variations within individual microarray hybridizations are often observed, but the mechanisms underpinning this positional bias remain incompletely explained.

    METHODOLOGY/PRINCIPAL FINDINGS:

    This study identifies and rationalizes a systematic spatial bias in the intensity of surface hybridization, characterized by markedly increased signal intensity of spots located at the boundaries of the spotted areas of the microarray slide. Combining observations from a simplified single-probe block array format with predictions from a mathematical model, the mechanism responsible for this biasis found to be a position-dependent variation in lateral diffusion of target molecules. Numerical simulations reveal a strong influence of microarraywell geometry on the spatial bias.

    CONCLUSIONS:

    Reciprocal adjustment of the size of the microarray hybridization chamber to the area of surface-bound probes is a simple and effective measure to minimize or eliminate the diffusion-based bias, resulting in increased uniformity and accuracy of quantitative DNA microarrayhybridization.

  • Mycobacterium avium infections of Acanthamoeba strains: host strain variability, grazing-acquired infections, and altered dynamics of inactivation with monochloramine

    Berry D, Horn M, Xi C, Raskin L
    2010 - Appl. Environ. Microbiol., 76: 6685-6688

    Abstract: 

    Stable Mycobacterium avium infections of several Acanthamoeba strains were characterized by increased infection resistance of recent environmental isolates and reduced infectivity in the presence of other bacteria. Exposure of M. avium in co-culture with Acanthamoeba castellanii to monochloramine yielded inactivation kinetics strikingly similar to those observed for A. castellanii alone.

Book chapters and other publications

5 Publications found
  • Hidden potential: Diet-driven changes in redox level shape the rumen microbiome

    2017 - Environmental Microbiology, 1: 19-20
  • The unexpected versatility of the cellulosome

    2017 - Environmental Microbiology, 1: 13-14
  • Principles of Systems Biology, No. 13 - A pathogen-resistant designer microbiota

    Stecher B, Clavel T, Loy A, Berry D
    2017 - Cell Systems, 4: 3-6
  • Making It Stick: A Compelling Case for Precision Microbiome Reconstitution

    2016 - Cell Host & Microbe, 20: 415-417

    Abstract: 

    Modification of the intestinal microbiome is an emerging target to improve health and prevent or treat a number of diseases. In this issue of Cell Host & Microbe, Maldonado-Gomez et al. (2016) uncover the basic principles that govern the successful establishment and persistence of an exogenously introduced gut bacterium.

  • The emerging view of Firmicutes as key fibre degraders in the human gut

    2016 - Environ. Microbiol., 7: 2081-3